Abstract:
:Islet transplantation has made major progress to treat patients with type 1 diabetes. Islet mass and quality are critically important to ensure successful transplantation. Currently, islet status is evaluated using insulin secretion, oxygen consumption rate, or adenosine triphosphate (ATP) measurement. These parameters are evaluated independently and do not effectively predict islet status post-transplant. Therefore, assessing human pancreatic islets by encompassing ATP, DNA, insulin, and protein content from a single tissue sample would serve as a better predictor for islet status. In this study, a single step procedure for extracting ATP, DNA, insulin, and protein content from human pancreatic islets was described and the biomolecule contents were quantified. Additionally, different mathematical calculations integrating total ATP, DNA, insulin, and protein content were randomly tested under various conditions to predict islet status. The results demonstrated that the ATP assay was efficient and the biomolecules were effectively quantified. Furthermore, the mathematical formula we developed could be optimized to predict islet status. In conclusion, our results indicate a proof-of-concept that a simple logarithmic formula can predict overall islet status for various conditions when total islet ATP, DNA, insulin, and protein content are simultaneously assessed from a single tissue sample.
journal_name
Cell Tissue Bankjournal_title
Cell and tissue bankingauthors
Qi M,Bilbao S,Forouhar E,Kandeel F,Al-Abdullah IHdoi
10.1007/s10561-017-9659-9subject
Has Abstractpub_date
2018-03-01 00:00:00pages
77-85issue
1eissn
1389-9333issn
1573-6814pii
10.1007/s10561-017-9659-9journal_volume
19pub_type
杂志文章abstract::During August and September of 2013, temperature data loggers were shipped to and from an AATB accredited and FDA registered allograft tissue processing facility in Belgrade, MT (Bacterin International, Inc.) to five warm climate cities (Dallas, TX, El Paso, TX, New Orleans, LA, Phoenix, AZ, and Tampa, FL). Shipping d...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-016-9578-1
更新日期:2016-12-01 00:00:00
abstract::A thick gingival biotype is a requisite for good periodontal health. It has important role in resisting trauma and subsequent gingival recession. The gingival thickness is a significant predictor of clinical outcome of periodontal surgeries. Various surgical procedures are used to increase the gingival thickness. The ...
journal_title:Cell and tissue banking
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10561-015-9524-7
更新日期:2016-03-01 00:00:00
abstract::Recently Bulgarian Bone Marrow Donors Registry (BBMDR) has been established and since August 2005 it has been a member of Bone Marrow Donors Worldwide. Currently the number of healthy donors included in the BBMDR is relatively low. All donors included in the BBMDR are typed for HLA-A, -B, -DRB loci. Phylogenetic analy...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-007-9046-z
更新日期:2008-12-01 00:00:00
abstract::This is a report of a workshop held on the establishment of human research tissue banking which was held in Levi, Finland 21-24 March 2002. There were 21 participants from 7 European countries. This meeting was attended by representatives from academia, research tissue banks and from the Biotech and Pharmaceutical Ind...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/A:1022811232250
更新日期:2002-01-01 00:00:00
abstract::Nowadays, there are four types of meniscal allografts known: fresh, cryopreserved, deep-frozen and lyophilized ones but only two of them are widely used in clinical practice. Use of different types of meniscal allografts still remains controversial due to preparation method, their biomechanical properties as well as c...
journal_title:Cell and tissue banking
pub_type: 杂志文章,评审
doi:10.1007/s10561-013-9396-7
更新日期:2014-09-01 00:00:00
abstract::The aim of the present study was to compare the effects of two freezing methods, vapor phase and very rapid freezing, with and without cryoprotectant on semen parameters in men with normal semen criteria. Cryopreservation was done on semen samples from 31 men by two methods of vapor phase freezing and very rapid freez...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-012-9351-z
更新日期:2013-12-01 00:00:00
abstract::Cryopreserved (CryoPA) and Glycerol-preserved (GPA) skin allografts are commonly used in the treatment of severe burn injuries. However, comparable data on their differences in clinical outcome is scarce. This retrospective review aims to study the effect of allograft viability on clinical outcomes. The records of 48 ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-011-9254-4
更新日期:2012-06-01 00:00:00
abstract::Since there is no upper age limit for general organ donation, unlike heart valve donation, and since a quarter of all organ donors are 65 years and older, we examined whether the heart valves from these donors are suitable as allografts. In the period 1999-2004 the aortic valve and pulmonary valve of 100 organ donors ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-007-9052-1
更新日期:2008-03-01 00:00:00
abstract::Blood samples collected from deceased tissue donors for mandatory transfusion microbiology testing may be taken either at the time of tissue donation, or residual samples may be retrieved from hospital laboratories where they were originally used for ante-mortem tests. In the latter case, sample labelling may not conf...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-008-9071-6
更新日期:2008-12-01 00:00:00
abstract::To analyze the data of the adverse events collected in a single major eye bank (EFS Bourgogne Franche Comté, Besançon, France) for the year 2013 and to report the French data of biovigilance provided by the French National Agency for Medicines and Health Products Safety (ANSM) between 2010 and 2013. we have set up a s...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-016-9593-2
更新日期:2017-03-01 00:00:00
abstract::Whilst some of the assays used for serological screening of post-mortem blood samples from deceased tissue donors in some countries have been specifically validated by the manufacturer for this purpose, a significant number of those currently in use globally have not. Although specificity has previously been considere...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-010-9175-7
更新日期:2011-05-01 00:00:00
abstract::The goal of periodontal therapy is to prevent periodontal disease progression and to regenerate lost periodontal tissues. Various bone grafts are used alone or in combination with other treatment modalities for periodontal therapy. In literature no reports are presented with block allografts in the treatment of intrab...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-018-09744-5
更新日期:2019-02-11 00:00:00
abstract::This study investigated cryopreserved pulmonary homograft (CPA) structural integrity after prolonged cold ischemic harvesting times in a juvenile sheep model. Three groups with different post-mortem cold ischemic harvesting times were studied, i.e. Group 1 (24 h, n = 10); group 2 (48 h, n = 10); group 3 (72 h, n = 10)...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-015-9500-2
更新日期:2015-12-01 00:00:00
abstract::The purpose of the present research was: 1. To investigate the opportunity of application of allogen osteoplastic materials such as Lioplast, received in Samara Tissue Bank of Samara State Medical University. 2. To work out a new technique of producing lyophilized allogen osteoplastic materials, such as Lioplast, with...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-004-7254-3
更新日期:2005-01-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) are used for tissue regeneration in several pathological conditions, including autoimmune diseases. However, the optimal sources and culture requirements for these cells are still under investigation. Here, we compared mRNA expression in dermal MSCs (DMSCs) at passage (P) 3 and P5 to prov...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-017-9672-z
更新日期:2018-09-01 00:00:00
abstract::In order to guarantee the required level of quality for our Bone &Tissue Banking, we evaluated a new CE marked container (CELLFLEX MacoPharma), for packing, transport, processing and storage of bones for therapeutic use. The use of CE marked containers is mandatory for organ and tissue containers (Medical Device Direc...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-004-1090-3
更新日期:2004-01-01 00:00:00
abstract::Human amniotic membrane (hAM) represents a tissue that is well established as biomaterial in the clinics with potential for new applications in regenerative medicine. For tissue engineering (TE) strategies, cells are usually combined with inductive factors and a carrier substrate. We have previously recognized that hA...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-014-9454-9
更新日期:2014-06-01 00:00:00
abstract::Although allografts for anterior cruciate ligament (ACL) replacement have shown advantages compared to autografts, their use is limited due to the risk of disease transmission and the limitations of available sterilization methods. Gamma sterilization has shown detrimental effects on graft properties at the high doses...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-011-9289-6
更新日期:2012-08-01 00:00:00
abstract::In Malaysia, tissue banking activities began in Universiti Sains Malaysia (USM) Tissue Bank in early 1990s. Since then a few other bone banks have been set up in other government hospitals and institutions. However, these banks are not governed by the national authority. In addition there is no requirement set by the ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-010-9188-2
更新日期:2010-11-01 00:00:00
abstract::The increased use of allograft tissue for musculoskeletal repair has brought more focus to the safety of allogenic tissue and the efficacy of various sterilization techniques. Gamma irradiation is an effective method for providing terminal sterilization to biological tissue, but it is also reported to have deleterious...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-008-9069-0
更新日期:2008-12-01 00:00:00
abstract::This study was conducted to evaluate the effect of combined cysteine and glutathione in soy lecithin-based semen extender on post-thawed ram sperm quality. A total of 28 ejaculates were collected twice a week (from four rams) during breeding season. In each replicate, semen samples (n = 4, one ejaculate for each ram) ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-014-9488-z
更新日期:2015-09-01 00:00:00
abstract::To vitrify human amniotic membrane specimens so that the maximum of epithelial cells survives in order to develop a procedure for the eventual vitrification of posterior corneal lamellae without using cryoprotective agents. To assess different methods of tissue sample preparation preceding vitrification. In group 1, t...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-013-9388-7
更新日期:2014-03-01 00:00:00
abstract::This study aimed to optimise methods of cryopreserving human articular cartilage (AC) tissue for the isolation of late chondrocytes. Human AC specimens from osteoarthritis patients who had undergone total knee replacement were used to optimise the chondrocyte isolation process and the choice of cryoprotective agent (C...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-012-9328-y
更新日期:2013-06-01 00:00:00
abstract::The regulatory framework of tissue banking introduces a number of requirements for monitoring cleanrooms for processing tissue or cell grafts. Although a number of requirements were clearly defined, some requirements are open for interpretation. This study aims to contribute to the interpretation of GMP or GTP guideli...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-012-9355-8
更新日期:2013-12-01 00:00:00
abstract::Terminal sterilization of musculoskeletal allografts by gamma radiation minimizes the risk of disease transmission but impairs allograft mechanical properties. Commonly employed crosslinking agents can sterilize tissues without affecting mechanical properties adversely; however, these agents are toxic. Genipin is repo...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-012-9335-z
更新日期:2013-09-01 00:00:00
abstract::Rheumatoid arthritis (RA) is the most common degenerative arthritic cartilage and represents a disease where the prospect of stem cell therapy offers considerable hope. Currently, bone marrow (BM) represents the major source of mesenchymal stem cells (MSCs) for cell therapy. In the pathology of RA, the pro-inflammator...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-012-9354-9
更新日期:2013-12-01 00:00:00
abstract::The amniotic membranes were collected from the placentae of selected and screened donors. Processing was done by washing the fresh amniotic membrane successively in sterile saline, 0.05% sodium hypochlorite solution and sterile distilled water until it was completely cleared of blood particles. The membranes were ster...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/B:CATB.0000034077.05000.29
更新日期:2004-01-01 00:00:00
abstract::In India, the procurement of tissues for transplantation is governed by the Transplantation of Human Organs Act, 1994. However, although this law exists, it is primarily applied to organ transplantation and rules and regulations that are specific to tissue banking have yet to be developed.The Tata Memorial Hospital (T...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/B:CATB.0000007026.00604.97
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:The role of bone and tissue banking is well known to meet the ever-growing need of bone and soft tissue allografts. Strict guidelines have been established to ensure high standard and minimize complications related to bone transplantation. METHODS:The Bone Bank in King Faisal Specialist Hospital and Researc...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-020-09868-7
更新日期:2020-10-04 00:00:00
abstract::The amnionic membrane is a rich source of multipotent mesenchymal stromal cells (hAMSC), which are readily available and show a potential use in regenerative medicine and tissue engineering. Before these cells can be applied clinically, careful characterization is necessary, especially as primary cells are known to ch...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-013-9415-8
更新日期:2014-06-01 00:00:00