Does prolonged post-mortem cold ischemic harvesting time influence cryopreserved pulmonary homograft tissue integrity?

Abstract:

:This study investigated cryopreserved pulmonary homograft (CPA) structural integrity after prolonged cold ischemic harvesting times in a juvenile sheep model. Three groups with different post-mortem cold ischemic harvesting times were studied, i.e. Group 1 (24 h, n = 10); group 2 (48 h, n = 10); group 3 (72 h, n = 10). In each group, 5 CPAs were studied in vitro after cryopreservation and thawing. The other 5 CPAs were implanted in juvenile sheep for a minimum of 180 days. Serology samples were obtained and echocardiography was performed before euthanasia. Hematoxylin and eosin (H&E), scanning electron microscopy (SEM), von Kossa, Picrosirius red, α-actin, immunohistochemistry [von Willebrand factor (vWF), CD4, CD31 and CD34] and calcium content analyses were performed on explanted CPAs. The in vitro and in vivo studies failed to demonstrate any change in tensile strength, Young's Modulus and thermal denaturation (Td) results between the groups. SEM demonstrated a reduction in endothelial cells (50 % at 24 h, 60.9 % at 48 h and 40.9 % at 72 h), but H&E could not demonstrate autolysis in any CPA in vitro. All cultures were negative. In the explanted groups, IgE, IgM and IgG results were inconclusive. Echocardiography demonstrated normal valve function in all groups. H&E and Picrosirius red staining confirmed tissue integrity. vWF, CD31 and CD34 staining confirmed a monolayer of endothelial cells in all explanted valves. Calcium content of explanted CPA leaflets was similar. This experimental study supports the concept of prolonging the cold ischemic harvesting time of cryopreserved homografts to reduce homograft shortage.

journal_name

Cell Tissue Bank

journal_title

Cell and tissue banking

authors

Smit FE,Bester D,van den Heever JJ,Schlegel F,Botes L,Dohmen PM

doi

10.1007/s10561-015-9500-2

subject

Has Abstract

pub_date

2015-12-01 00:00:00

pages

531-44

issue

4

eissn

1389-9333

issn

1573-6814

pii

10.1007/s10561-015-9500-2

journal_volume

16

pub_type

杂志文章
  • A new standardized immunofluorescence method for potency quantification (SMPQ) of human conjunctival cell cultures.

    abstract::The aim of this study is to set up a standardized and reproducible method to determine the potency (= stem cell content) of human conjunctival cell cultures by means of immunofluorescence-based analyses. This will help the development of new Advanced Therapy Medicinal Products (ATMPs) to use in future cell therapy cli...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-020-09874-9

    authors: Bertolin M,Breda C,Ferrari S,Lamon M,Ponzin D,Ferrari B,Barbaro V

    更新日期:2020-10-13 00:00:00

  • Corneal transplantation: study of the data of a regional eye bank for the year 2013 and analysis of the evolution of the adverse events reported in France since 2010.

    abstract::To analyze the data of the adverse events collected in a single major eye bank (EFS Bourgogne Franche Comté, Besançon, France) for the year 2013 and to report the French data of biovigilance provided by the French National Agency for Medicines and Health Products Safety (ANSM) between 2010 and 2013. we have set up a s...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-016-9593-2

    authors: Gauthier AS,Castelbou M,Garnier MB,Pizzuto J,Roux S,Gain P,Pouthier F,Delbosc B

    更新日期:2017-03-01 00:00:00

  • Comparison of impact of two decontamination solutions on the viability of the cells in human amnion.

    abstract::Human amniotic membrane (HAM) is used as an allograft in regenerative medicine or as a source of pluripotent cells for stem cell research. Various decontamination protocols and solutions are used to sterilize HAM before its application, but little is known about the toxicity of disinfectants on HAM cells. In this stud...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-017-9636-3

    authors: Smeringaiova I,Trosan P,Mrstinova MB,Matecha J,Burkert J,Bednar J,Jirsova K

    更新日期:2017-09-01 00:00:00

  • Enhancing gingival biotype through chorion membrane with innovative step in periodontal pocket therapy.

    abstract::A thick gingival biotype is a requisite for good periodontal health. It has important role in resisting trauma and subsequent gingival recession. The gingival thickness is a significant predictor of clinical outcome of periodontal surgeries. Various surgical procedures are used to increase the gingival thickness. The ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s10561-015-9524-7

    authors: Kothiwale S,Rathore A,Panjwani V

    更新日期:2016-03-01 00:00:00

  • Negative effect of combined cysteine and glutathione in soy lecithin-based extender on post-thawed ram spermatozoa.

    abstract::This study was conducted to evaluate the effect of combined cysteine and glutathione in soy lecithin-based semen extender on post-thawed ram sperm quality. A total of 28 ejaculates were collected twice a week (from four rams) during breeding season. In each replicate, semen samples (n = 4, one ejaculate for each ram) ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-014-9488-z

    authors: Zhandi M,Sharafi M

    更新日期:2015-09-01 00:00:00

  • Defined serum- and xeno-free cryopreservation of mesenchymal stem cells.

    abstract::Mesenchymal stem cells (MSCs) have vast potential in cell therapy, and are experimentally used in the clinic. Therefore, it is critical to find a serum- and xeno-free cryopreservation method. The aim of this study was to compare two serum- and xeno-free cryoprotectants for MSCs. Adipose tissue MSCs (Ad-MSCs) and bone ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-014-9463-8

    authors: Al-Saqi SH,Saliem M,Quezada HC,Ekblad Å,Jonasson AF,Hovatta O,Götherström C

    更新日期:2015-06-01 00:00:00

  • Reliability of cartilage digestion and FDA-EB fluorescence staining for the detection of chondrocyte viability in osteochondral grafts.

    abstract::The purpose of this study is to evaluate the reliability of cartilage digestion and fluorescein diacetate-ethidium bromide (FDA-EB) fluorescence staining for the detection of chondrocyte viability in osteochondral grafts. Sixteen fresh osteochondral grafts were harvested from pig knee condyles, and the articular carti...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-018-9685-2

    authors: Qi J,Cao F,Han Y,Xie D,Song H,Chen B,Zhou L

    更新日期:2018-09-01 00:00:00

  • Banking of fresh-frozen prostate tissue using the alternate mirror image protocol: methods, validation, and impact on the pathological prognostic parameters in radical prostatectomy.

    abstract::We evaluated the value of the 'alternative slices mirror image method' used in prostate tissue banking in terms of predicting the sampling of cancerous tissue while preserving the pathological prognostic information. The concordance of diagnosis between banked sections and their mirror image paraffin- sections was stu...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-011-9284-y

    authors: Brimo F,Sircar K,Chevalier S,Saad F,Lacombe L,Têtu B,Scarlata E,Aprikian A

    更新日期:2012-12-01 00:00:00

  • Effect of thermodisinfection on mechanic parameters of cancellous bone.

    abstract::Revision surgery of joint replacements is increasing and raises the demand for allograft bone since restoration of bone stock is crucial for longevity of implants. Proceedings of bone grafts influence the biological and mechanic properties differently. This study examines the effect of thermodisinfection on mechanic p...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-016-9567-4

    authors: Fölsch C,Kellotat A,Rickert M,Ishaque B,Ahmed G,Pruss A,Jahnke A

    更新日期:2016-09-01 00:00:00

  • Risk factors associated with contamination of allograft valves in a tissue bank.

    abstract::The contamination of the transport solution used in cardiovascular allografts can occur from different sources. Risk factors associated with positive microbiological test of transport solution have not been reported previously. This study aimed to determine the risk factor for contamination of transport solution used ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-019-09754-x

    authors: Sawa B,Ribeiro VST,Kraft L,Wollmann LC,Pegoraro D,Suss PH,Tuon FF

    更新日期:2019-03-01 00:00:00

  • Setting up a Tissue Bank in India: The Tata Memorial Hospital Experience.

    abstract::In India, the procurement of tissues for transplantation is governed by the Transplantation of Human Organs Act, 1994. However, although this law exists, it is primarily applied to organ transplantation and rules and regulations that are specific to tissue banking have yet to be developed.The Tata Memorial Hospital (T...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1023/B:CATB.0000007026.00604.97

    authors: Gajiwala AL

    更新日期:2003-01-01 00:00:00

  • Devastating endophthalmitis caused by Serratia marcescens in two recipients after transplantation of corneal grafts from the same donor.

    abstract::We report two cases of severe endophthalmitis, which were caused by Serratia marcescens, and developed in the immediate postoperative period in two recipients of corneal grafts from the same donor. The cause of the donor's death was massive CVA. He had been on mechanical ventilation for 12 days before he died, and had...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1023/A:1021839711471

    authors: Levartovsky S,Lazarovich A

    更新日期:2002-01-01 00:00:00

  • Confirmation of cadaveric blood sample identity by DNA profiling using Short Tandem Repeat (STR) analysis.

    abstract::Blood samples collected from deceased tissue donors for mandatory transfusion microbiology testing may be taken either at the time of tissue donation, or residual samples may be retrieved from hospital laboratories where they were originally used for ante-mortem tests. In the latter case, sample labelling may not conf...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-008-9071-6

    authors: Warwick RM,Rushambuza FG,Brown J,Patel R,Tabb S,Poniatowski S,Ranson AJ,Brown CJ

    更新日期:2008-12-01 00:00:00

  • Analysis of impact on tissue activity during COVID-19 outbreak: a survey of 8 banks in Spain.

    abstract::On March 19 World Health Organization declare the pandemic situation by outbreak coronavirus disease 2019 in the world. The pressure on the health care system has been very high in several countries. Spanish National Transplant Organization (ONT) have made many efforts in maintaining transplantation activity. Although...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章,多中心研究

    doi:10.1007/s10561-020-09853-0

    authors: Villalba R,Santos S,Martinez MJ,Díaz M,Pevida M,Cemborain A,Casares C,Mirabet V

    更新日期:2020-12-01 00:00:00

  • Maternal predictors and quality of umbilical cord blood units.

    abstract::The aim of the study was to determine the relationship between the maternal age at delivery and selected properties of the cord blood stem cells. The study included 50 pregnant women aged between 18 and 38 years in which spontaneous labors or elective cesarean sections were performed. Umbilical cord blood was collecte...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-017-9657-y

    authors: Bielec-Berek B,Jastrzębska-Stojko Ż,Drosdzol-Cop A,Jendyk C,Boruczkowski D,Ołdak T,Nowak-Brzezińska A,Stojko R

    更新日期:2018-03-01 00:00:00

  • Comparison between isolation protocols highlights intrinsic variability of human umbilical cord mesenchymal cells.

    abstract::Mesenchymal stem cells (MSCs) though multipotent exhibit limited lifespan in vitro, with progressive reduction in capacity for self-renewal leading to irreversible arrest of cell division, which limits their use for therapeutic purposes. Human umbilical cord wall MSCs are easy to process and proliferate rapidly in cul...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-015-9525-6

    authors: Paladino FV,Peixoto-Cruz JS,Santacruz-Perez C,Goldberg AC

    更新日期:2016-03-01 00:00:00

  • A future vision of the International Atomic Energy Agency (IAEA) program on radiation and tissue banking for Asia and the Pacific and Latin American regions.

    abstract::In order to solve some of the problems that are affecting tissue banking activities in the world, a new program/project proposal could be prepared by the IAEA and interested Member States in order to implement it in 2009. The main objective of the new program/project proposal could be the following: To consolidate tis...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-008-9092-1

    authors: Morales Pedraza J

    更新日期:2009-05-01 00:00:00

  • A banking strategy toward customized therapy in breast cancer.

    abstract::In breast cancer, various clinical parameters are assessed to define clinical stage and thus obtain a more accurate prognosis. However, banks of tumor tissues are an important source of material for studies of risk of recurrence and of features governing clinical outcome in breast cancer. Although the heterogeneous ch...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-009-9124-5

    authors: Nardone A,Cavaliere C,Corvigno S,Limite G,De Placido S,Veneziani BM

    更新日期:2009-11-01 00:00:00

  • Comparative evaluation of bioburden and sterility of indigenously prepared bone allograft with and without gentamicin.

    abstract::During bone allograft processing, despite stringent donor screening and use of aseptic techniques, microbial invasion may occur due to the porous nature of the graft and cause potentially fatal infections. The aim of the present study was to prepare bone allograft with and without gentamicin and to compare bioburden a...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-019-09763-w

    authors: Makker K,Lamba AK,Faraz F,Tandon S,Sheikh Ab Hamid S,Aggarwal K,Chowdhri K

    更新日期:2019-06-01 00:00:00

  • Porcine bone grafts defatted by lipase: efficacy of defatting and assessment of cytocompatibility.

    abstract::Defatting is an important procedure for the preparation of bone grafts because lipids in bone grafts strongly influence the osteointegration. Lipases have been widely used in different fields. However, study on the application to defatting process for bone grafts preparation has never been found so far. In this study,...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-013-9391-z

    authors: Zhang N,Zhou M,Zhang Y,Wang X,Ma S,Dong L,Yang T,Ma L,Li B

    更新日期:2014-09-01 00:00:00

  • ISBT 128: a global information standard.

    abstract::ISBT 128 is the global standard for the identification, labeling and information transfer of human blood, cell, tissue and organ products across international borders and disparate health care systems. The system has been designed and perfected over almost two decades to ensure accuracy, safety and efficiency for the ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-010-9196-2

    authors: Distler P

    更新日期:2010-11-01 00:00:00

  • When do tissues and cells become products? Regulatory oversight of emerging biological therapies.

    abstract::Although therapeutics derived from biological sources have been subjected to regulatory oversight for some time, the products used in transplantation procedures have historically been exempt from this oversight. These products have been viewed as being part of medical practice rather than as the result of mainstream p...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章,评审

    doi:10.1007/s10561-006-9012-1

    authors: Farrugia A

    更新日期:2006-01-01 00:00:00

  • Biocompatibility Studies of a New Biosynthetic Dermal Substitute Based on Collagen/Hyaluronan Conjugate.

    abstract::The use of glycosaminoglycans (GAGs) as the second macromolecular component with collagen for the membrane preparation is based on the idea to create a biodegradable scaffold for new tissue. A hybrid collagen/hyaluronan membrane with specific bubble macrostructure was designed to serve as a synthetic dermal substitute...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1023/a:1010181925236

    authors: Koller J,Bakos D,Sadlonová I I

    更新日期:2000-01-01 00:00:00

  • Tissue allograft coding and traceability in USM Tissue Bank, Malaysia.

    abstract::In Malaysia, tissue banking activities began in Universiti Sains Malaysia (USM) Tissue Bank in early 1990s. Since then a few other bone banks have been set up in other government hospitals and institutions. However, these banks are not governed by the national authority. In addition there is no requirement set by the ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-010-9188-2

    authors: Sheikh Ab Hamid S,Abd Rahman MN

    更新日期:2010-11-01 00:00:00

  • Risk factors for rejection for morphological reasons of heart valves for transplantation.

    abstract::The study aim was to identify risk factors for morphological rejection of aortic and pulmonary valves for transplantation that could be used to optimize donor selection. The files of all Dutch heart valve donors, donating in a 2.5 years period, whose hearts were processed at Heart Valve Bank Rotterdam, were reviewed f...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-011-9267-z

    authors: van Wijk MJ,van den Bogaerdt A,Bokhorst AG

    更新日期:2013-03-01 00:00:00

  • Isolation, characterisation and comparative analysis of human umbilical cord vein perivascular cells and cord blood mesenchymal stem cells.

    abstract::Perivascular cells are known to be ancestors of mesenchymal stem cells (MSCs) and can be obtained from heart, skin, bone marrow, eye, placenta and umbilical cord (UC). However detailed characterization of perivascular cells around the human UC vein and comparative analysis of them with MSCs haven't been done yet. In t...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-015-9542-5

    authors: Gökçinar-Yagci B,Özyüncü Ö,Çelebi-Saltik B

    更新日期:2016-06-01 00:00:00

  • Experience gained during the long term cultivation of keratinocytes for treatment of burns patients.

    abstract::Both allogenic and autologous cultured skin cells have been used clinically on burn patients. In vitro cultivation of human keratinocytes has been routinely provided by the Central Tissue Bank in Bratislava since 1996, with an average annual production of around 7,000 cm(2). Keratinocytes have been cultivated using a ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-011-9275-z

    authors: Dragúňová J,Kabát P,Koller J,Jarabinská V

    更新日期:2012-08-01 00:00:00

  • Mesenchymal stem cells for chronic wounds therapy.

    abstract::Wound healing is a complex process that involves interaction of soluble mediators, extracellular matrix and infiltrating blood cells. Chronic and non-healing skin defects contribute significantly to morbidity and mortality of many patients. Recently, despite the current medical progress, the chronic and non-healing wo...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章,评审

    doi:10.1007/s10561-014-9440-2

    authors: Zahorec P,Koller J,Danisovic L,Bohac M

    更新日期:2015-03-01 00:00:00

  • Potential of 5-azacytidine induction decidual stromal cells from maternal human term placenta towards cardiomyocyte-like cells in serum-free medium.

    abstract::Decidual stromal cells (DSCs) from maternal term placenta represent a potential source of cells for the treatment of cardiovascular and graft-versus-host diseases. However, it is not clear whether DSCs could be induced towards cardiomyocyte-like differentiation. We chose the placentas which should bred male new-baby. ...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-015-9493-x

    authors: Chen G,Yue A,Ruan Z,Yin Y,Wang R,Ren Y,Zhu L

    更新日期:2015-09-01 00:00:00

  • Activation of caspases in human spermatozoa during cryopreservation--an immunoblot study.

    abstract::Cellular stress to ejaculated spermatozoa such as cryopreservation is known to induce caspase-derived, apoptotic signaling. Therefore, the proenzymes and active forms of caspases 1, 3, 8 and 9 were examined by western blot technique in unfrozen and frozen human spermatozoa of infertility patients and of healthy donors...

    journal_title:Cell and tissue banking

    pub_type: 杂志文章

    doi:10.1007/s10561-005-0276-7

    authors: Wündrich K,Paasch U,Leicht M,Glander HJ

    更新日期:2006-01-01 00:00:00