Abstract:
:Whilst some of the assays used for serological screening of post-mortem blood samples from deceased tissue donors in some countries have been specifically validated by the manufacturer for this purpose, a significant number of those currently in use globally have not. Although specificity has previously been considered a problem in the screening of such samples, we believe that ensuring sensitivity is more important. The aim of this study was to validate a broader range of assays for the screening of post-mortem blood samples from deceased tissue donors. Six microplate immunoassays currently in use within National Health Service Blood and Transplant (NHSBT) for the screening of blood, tissue and stem cell donations were included. Representative samples from confirmed positive donors were titrated in screen negative post-mortem samples in parallel with normal pooled negative serum to determine if there was any inhibition with the post-mortem samples. There were no significant differences seen (P < 0.005) between the dilution curves obtained for the positive samples diluted in post-mortem samples and normal pooled sera. Although small numbers of samples were studied, it can be surmised that the post-mortem blood samples from deceased tissue donors, collected according to United Kingdom guidelines, are a suitable substrate for the assays evaluated. No diminution of reactivity was seen when dilution with sera from deceased donors was compared to dilution using pooled serum from live donors. In the absence of genuine low titre positive post-mortem samples, the use of samples spiked with various levels of target material provides a means of qualifying serological screening assays used by NHSBT for the screening of post-mortem blood samples from deceased tissue donors.
journal_name
Cell Tissue Bankjournal_title
Cell and tissue bankingauthors
Kitchen AD,Newham JAdoi
10.1007/s10561-010-9175-7subject
Has Abstractpub_date
2011-05-01 00:00:00pages
117-24issue
2eissn
1389-9333issn
1573-6814journal_volume
12pub_type
杂志文章abstract::A thick gingival biotype is a requisite for good periodontal health. It has important role in resisting trauma and subsequent gingival recession. The gingival thickness is a significant predictor of clinical outcome of periodontal surgeries. Various surgical procedures are used to increase the gingival thickness. The ...
journal_title:Cell and tissue banking
pub_type: 杂志文章,随机对照试验
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abstract::We report two cases of severe endophthalmitis, which were caused by Serratia marcescens, and developed in the immediate postoperative period in two recipients of corneal grafts from the same donor. The cause of the donor's death was massive CVA. He had been on mechanical ventilation for 12 days before he died, and had...
journal_title:Cell and tissue banking
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doi:10.1007/s10561-019-09754-x
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journal_title:Cell and tissue banking
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journal_title:Cell and tissue banking
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journal_title:Cell and tissue banking
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journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-013-9388-7
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journal_title:Cell and tissue banking
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journal_title:Cell and tissue banking
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journal_title:Cell and tissue banking
pub_type: 杂志文章
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journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/B:CATB.0000007039.98845.bd
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journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-015-9500-2
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doi:10.1007/s10561-020-09868-7
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abstract::Since there is no upper age limit for general organ donation, unlike heart valve donation, and since a quarter of all organ donors are 65 years and older, we examined whether the heart valves from these donors are suitable as allografts. In the period 1999-2004 the aortic valve and pulmonary valve of 100 organ donors ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-007-9052-1
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doi:10.1007/s10561-020-09866-9
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journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-012-9325-1
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doi:10.1007/s10561-004-1090-3
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journal_title:Cell and tissue banking
pub_type: 杂志文章
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journal_title:Cell and tissue banking
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journal_title:Cell and tissue banking
pub_type: 杂志文章,评审
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journal_title:Cell and tissue banking
pub_type: 杂志文章
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journal_title:Cell and tissue banking
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journal_title:Cell and tissue banking
pub_type: 临床试验,杂志文章
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