Abstract：
:Human adipose-derived stem cells (hADSCs) have the ability to influence immune response, and hence are key cell sources for tissue repair and regeneration. In this study we explored the effect of continuous passage on the immunomodulatory properties of hADSCs to provide some advises for large-scale production of hADSCs for clinical applications. We found that after continuous passage, the specific surface markers expression levels as well as the adipogenic and osteogenic differentiation capacities of hADSCs had no obvious changes. However, the secretion levels of IL-10 and HGF reduced dramatically along with passage numbers. Furthermore, the INF-γ level was found higher in which medium peripheral blood mononuclear cells were co-cultured with hADSCs with higher passage numbers. Also, the in vivo experiments showed that the peritonitis model mice, which were injected with higher passage numbers of hADSCs, tended to have higher levels of inflammation. All these together indicated that continuous passage has only minor effect on the cell phenotypes but will impair the immunomodulatory properties of hADSCs. This suggests that hADSCs could be prepared by continuous passage, but only those cells of lower passage numbers would be ideal therapeutic tools.
journal_name
Cell Tissue Bankjournal_title
Cell and tissue bankingauthors
Wang X,Liu C,Li S,Xu Y,Chen P,Liu Y,Ding Q,Wahapu W,Hong B,Yang Mdoi
10.1007/s10561-014-9451-zsubject
Has Abstractpub_date
2015-03-01 00:00:00pages
143-50issue
1eissn
1389-9333issn
1573-6814journal_volume
16pub_type
杂志文章abstract：:Allogeneic mandibular bone processed by the deep frozen method was used as a biologic crib for mandibular reconstruction. The allogeneic mandible is biocompatible, bioresorbable, of low antigenicity and provides the morphology for symphysis contour, angle of mandible and dental arch form. The particulate cancellous bo...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1023602717328
更新日期：2002-01-01 00:00:00
abstract：:The regulatory framework of tissue banking introduces a number of requirements for monitoring cleanrooms for processing tissue or cell grafts. Although a number of requirements were clearly defined, some requirements are open for interpretation. This study aims to contribute to the interpretation of GMP or GTP guideli...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-012-9355-8
更新日期：2013-12-01 00:00:00
abstract：:Freeze-dried dura mater allograft (FDDMA) was evaluated as a cost-effective collagenous barrier using an experimental animal model to determine its biocompatibility and 'cell-occlusivity' potential. The FDDMA was processed at the Civil Hospital Ahmedabad, in accordance with the guidelines provided by the South-Eastern...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/B:CATB.0000007039.98845.bd
更新日期：2003-01-01 00:00:00
abstract：:The Central Tissue Bank in Warsaw was established in 1963 and since then ionising radiation has been routinely applied to sterilise tissue grafts. Connective tissue grafts such as bone, cartilage, tendons, sclera, pericardium, skin, acellular dermis and amnion irradiated with a dose of 35 kGy in a (60)Co source and/or...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-005-0338-x
更新日期：2005-01-01 00:00:00
abstract：:Injured nerves cannot regenerate on their own, and a lack of engraftable human nerves has been a major obstacle in cell-based therapies for regenerating damaged nerves. A monolayer culture approach to obtain adherent neural stem cells from human embryonic stem cells (hESC-NSCs) was established, and the greatest number...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09816-5
更新日期：2020-06-01 00:00:00
abstract：:This study investigated cryopreserved pulmonary homograft (CPA) structural integrity after prolonged cold ischemic harvesting times in a juvenile sheep model. Three groups with different post-mortem cold ischemic harvesting times were studied, i.e. Group 1 (24 h, n = 10); group 2 (48 h, n = 10); group 3 (72 h, n = 10)...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-015-9500-2
更新日期：2015-12-01 00:00:00
abstract：:Quality control and standardized preservation methods are essential in the field of transplantation. The International Organization for Standardization (ISO) has established a common set of manufacturing, trade and communications standards that are applicable worldwide and that provide the basis of a quality plan for ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1011576332362
更新日期：2001-01-01 00:00:00
abstract：:During August and September of 2013, temperature data loggers were shipped to and from an AATB accredited and FDA registered allograft tissue processing facility in Belgrade, MT (Bacterin International, Inc.) to five warm climate cities (Dallas, TX, El Paso, TX, New Orleans, LA, Phoenix, AZ, and Tampa, FL). Shipping d...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-016-9578-1
更新日期：2016-12-01 00:00:00
abstract：:This study aimed to optimise methods of cryopreserving human articular cartilage (AC) tissue for the isolation of late chondrocytes. Human AC specimens from osteoarthritis patients who had undergone total knee replacement were used to optimise the chondrocyte isolation process and the choice of cryoprotective agent (C...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-012-9328-y
更新日期：2013-06-01 00:00:00
abstract：:In Malaysia, tissue banking activities began in Universiti Sains Malaysia (USM) Tissue Bank in early 1990s. Since then a few other bone banks have been set up in other government hospitals and institutions. However, these banks are not governed by the national authority. In addition there is no requirement set by the ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-010-9188-2
更新日期：2010-11-01 00:00:00
abstract：:Wound healing is a complex process that involves interaction of soluble mediators, extracellular matrix and infiltrating blood cells. Chronic and non-healing skin defects contribute significantly to morbidity and mortality of many patients. Recently, despite the current medical progress, the chronic and non-healing wo...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-014-9440-2
更新日期：2015-03-01 00:00:00
abstract：:The increased use of allograft tissue for musculoskeletal repair has brought more focus to the safety of allogenic tissue and the efficacy of various sterilization techniques. Gamma irradiation is an effective method for providing terminal sterilization to biological tissue, but it is also reported to have deleterious...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-008-9069-0
更新日期：2008-12-01 00:00:00
abstract：:Stem cell therapy is in constant need of new cell sources to conceive regenerative medicine approaches for diseases that are still without therapy. Scientists drew the attention toward amniotic membrane and amniotic fluid stem cells, since these sources possess many advantages: first of all as cells can be extracted f...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-014-9428-y
更新日期：2014-06-01 00:00:00
abstract：:Cellular stress to ejaculated spermatozoa such as cryopreservation is known to induce caspase-derived, apoptotic signaling. Therefore, the proenzymes and active forms of caspases 1, 3, 8 and 9 were examined by western blot technique in unfrozen and frozen human spermatozoa of infertility patients and of healthy donors...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-005-0276-7
更新日期：2006-01-01 00:00:00
abstract：:Recent studies have reported that stem cells can be isolated from a wide range of tissues including bone marrow, fatty tissue, adipose tissue and placenta. Moreover, several studies also suggest that skin dermis could serve as a source of stem cells, but are of unclear phenotype. Therefore, we isolated and investigate...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9471-8
更新日期：2015-06-01 00:00:00
abstract：:Blood samples collected from deceased tissue donors for mandatory transfusion microbiology testing may be taken either at the time of tissue donation, or residual samples may be retrieved from hospital laboratories where they were originally used for ante-mortem tests. In the latter case, sample labelling may not conf...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-008-9071-6
更新日期：2008-12-01 00:00:00
abstract：:This study was conducted to evaluate the effect of combined cysteine and glutathione in soy lecithin-based semen extender on post-thawed ram sperm quality. A total of 28 ejaculates were collected twice a week (from four rams) during breeding season. In each replicate, semen samples (n = 4, one ejaculate for each ram) ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9488-z
更新日期：2015-09-01 00:00:00
abstract：:Tissue banking is started in Thailand in 1979 at the Department of Orthopaedic Surgery, Siriraj Hospital, Bangkok. At that time tissues produced were freeze-dried bone allografts which were sterilized by ethylene oxide. In 1984, the freeze-dried tissue allograft project received an award from the National Research Cou...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1026542730541
更新日期：2000-01-01 00:00:00
abstract：:Since there is no upper age limit for general organ donation, unlike heart valve donation, and since a quarter of all organ donors are 65 years and older, we examined whether the heart valves from these donors are suitable as allografts. In the period 1999-2004 the aortic valve and pulmonary valve of 100 organ donors ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-007-9052-1
更新日期：2008-03-01 00:00:00
abstract：:There have been various studies about the acellular nerve allograft (ANA) as the alternative of autologous nerve graft in the treatment of peripheral nerve defects. As well as the decellularization process methods of ANA, the various enhancement methods of regeneration of the grafted ANA were investigated. The chondro...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-019-09770-x
更新日期：2019-06-01 00:00:00
abstract：:The goal of periodontal therapy is to prevent periodontal disease progression and to regenerate lost periodontal tissues. Various bone grafts are used alone or in combination with other treatment modalities for periodontal therapy. In literature no reports are presented with block allografts in the treatment of intrab...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-018-09744-5
更新日期：2019-02-11 00:00:00
abstract：:Mesenchymal stem cells (MSCs) though multipotent exhibit limited lifespan in vitro, with progressive reduction in capacity for self-renewal leading to irreversible arrest of cell division, which limits their use for therapeutic purposes. Human umbilical cord wall MSCs are easy to process and proliferate rapidly in cul...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-015-9525-6
更新日期：2016-03-01 00:00:00
abstract：BACKGROUND:The role of bone and tissue banking is well known to meet the ever-growing need of bone and soft tissue allografts. Strict guidelines have been established to ensure high standard and minimize complications related to bone transplantation. METHODS:The Bone Bank in King Faisal Specialist Hospital and Researc...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09868-7
更新日期：2020-10-04 00:00:00
abstract：:Recovered human and animal tissues are used extensively in surgery for wound repair and reconstruction. In preparation for the validation of chemical disinfection and radiation sterilization processes, studies were performed on the development and validation of quantitative bioburden recovery methods for human bone an...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-010-9238-9
更新日期：2012-03-01 00:00:00
abstract：BACKGROUND:Despite improved surgical techniques and the use of new medication, healing of corneal and conjunctival defects cannot always be achieved. In this connection the clinical use of human amnion, produced by different techniques, has represented a successful alternative for the past ten years. The purpose of the...
journal_title：Cell and tissue banking
pub_type： 临床试验,杂志文章
doi：10.1023/b:catb.0000022222.41304.de
更新日期：2004-01-01 00:00:00
abstract：:The aim of this study is to set up a standardized and reproducible method to determine the potency (= stem cell content) of human conjunctival cell cultures by means of immunofluorescence-based analyses. This will help the development of new Advanced Therapy Medicinal Products (ATMPs) to use in future cell therapy cli...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09874-9
更新日期：2020-10-13 00:00:00
abstract：:Cryopreserved (CryoPA) and Glycerol-preserved (GPA) skin allografts are commonly used in the treatment of severe burn injuries. However, comparable data on their differences in clinical outcome is scarce. This retrospective review aims to study the effect of allograft viability on clinical outcomes. The records of 48 ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9254-4
更新日期：2012-06-01 00:00:00
abstract：:Cell manufacturing for clinical applications is a unique form of biologics manufacturing that relies on maintenance of stringent work practices designed to ensure product consistency and prevent contamination by microorganisms or by another patient's cells. More extensive, prolonged laboratory processes involve greate...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-011-9276-y
更新日期：2012-12-01 00:00:00
abstract：:Musculo-skeletal allografts sterilized and deep frozen are among the most common human tissue to be preserved and utilized in modern medicine. The effects of a long deep freezing period on cortical bone has already been evaluated and found to be insignificant. However, there are no reports about the influences of a pr...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-008-9106-z
更新日期：2009-02-01 00:00:00
abstract：:Human amniotic membrane (hAM) represents a tissue that is well established as biomaterial in the clinics with potential for new applications in regenerative medicine. For tissue engineering (TE) strategies, cells are usually combined with inductive factors and a carrier substrate. We have previously recognized that hA...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9454-9
更新日期：2014-06-01 00:00:00
