Abstract:
:The aim of this study is to set up a standardized and reproducible method to determine the potency (= stem cell content) of human conjunctival cell cultures by means of immunofluorescence-based analyses. This will help the development of new Advanced Therapy Medicinal Products (ATMPs) to use in future cell therapy clinical studies when fewer cells are available to perform the quality controls. To achieve this purpose, a reference standard was investigated and the expression levels of ΔNp63α (considered as a marker of conjunctival stem cells) was correlated to cell size. The limbal hTERT cells were used as reference standard to define the expression value of ΔNp63α. The mean intensity value of limbal hTERT cells ranging between 15 and 20 µm in diameter was used to distinguish between ΔNp63α bright and not bright cells. As ΔNp63α bright expression was mainly seen in the smaller cell size group (10-15 µm), we defined as conjunctival stem cells (= potency) those cells which were bright and with sizes between 10 and 15 µm. Assays on cells from clonal analyses were used to validate the method, as they do allow to observe a decrease in potency (Holoclones > Meroclones > Paraclones). The stem cell content of conjunctival grafts was found to be 11.3% ± 5.0 compared to 21.9% ± 0.6, 9.0% ± 8.1 and 0% from Holoclones, Meroclones and Paraclones, respectively. This new method, here named as Standardized Method for Potency Quantification, will allow to detect the potency in conjunctival cell cultures, thus obtaining a quality control assay responding to the GMP standards required for ATMP release.
journal_name
Cell Tissue Bankjournal_title
Cell and tissue bankingauthors
Bertolin M,Breda C,Ferrari S,Lamon M,Ponzin D,Ferrari B,Barbaro Vdoi
10.1007/s10561-020-09874-9subject
Has Abstractpub_date
2020-10-13 00:00:00eissn
1389-9333issn
1573-6814pii
10.1007/s10561-020-09874-9pub_type
杂志文章abstract::Wound healing is a complex process that involves interaction of soluble mediators, extracellular matrix and infiltrating blood cells. Chronic and non-healing skin defects contribute significantly to morbidity and mortality of many patients. Recently, despite the current medical progress, the chronic and non-healing wo...
journal_title:Cell and tissue banking
pub_type: 杂志文章,评审
doi:10.1007/s10561-014-9440-2
更新日期:2015-03-01 00:00:00
abstract::Allogeneic mandibular bone processed by the deep frozen method was used as a biologic crib for mandibular reconstruction. The allogeneic mandible is biocompatible, bioresorbable, of low antigenicity and provides the morphology for symphysis contour, angle of mandible and dental arch form. The particulate cancellous bo...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/A:1023602717328
更新日期:2002-01-01 00:00:00
abstract::Whilst some of the assays used for serological screening of post-mortem blood samples from deceased tissue donors in some countries have been specifically validated by the manufacturer for this purpose, a significant number of those currently in use globally have not. Although specificity has previously been considere...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-010-9175-7
更新日期:2011-05-01 00:00:00
abstract::During bone allograft processing, despite stringent donor screening and use of aseptic techniques, microbial invasion may occur due to the porous nature of the graft and cause potentially fatal infections. The aim of the present study was to prepare bone allograft with and without gentamicin and to compare bioburden a...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-019-09763-w
更新日期:2019-06-01 00:00:00
abstract:BACKGROUND:Despite improved surgical techniques and the use of new medication, healing of corneal and conjunctival defects cannot always be achieved. In this connection the clinical use of human amnion, produced by different techniques, has represented a successful alternative for the past ten years. The purpose of the...
journal_title:Cell and tissue banking
pub_type: 临床试验,杂志文章
doi:10.1023/b:catb.0000022222.41304.de
更新日期:2004-01-01 00:00:00
abstract::Since there is no upper age limit for general organ donation, unlike heart valve donation, and since a quarter of all organ donors are 65 years and older, we examined whether the heart valves from these donors are suitable as allografts. In the period 1999-2004 the aortic valve and pulmonary valve of 100 organ donors ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-007-9052-1
更新日期:2008-03-01 00:00:00
abstract::Delivering the promise of personalised medicine is the challenge that the current generation of scientists face. The variations in human physiology and disease are considerable, and designing appropriate strategies to deliver what has been promised will require access to tissue from a large number of volunteers. The N...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-010-9212-6
更新日期:2011-02-01 00:00:00
abstract::The study aim was to identify risk factors for morphological rejection of aortic and pulmonary valves for transplantation that could be used to optimize donor selection. The files of all Dutch heart valve donors, donating in a 2.5 years period, whose hearts were processed at Heart Valve Bank Rotterdam, were reviewed f...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-011-9267-z
更新日期:2013-03-01 00:00:00
abstract::Rheumatoid arthritis (RA) is the most common degenerative arthritic cartilage and represents a disease where the prospect of stem cell therapy offers considerable hope. Currently, bone marrow (BM) represents the major source of mesenchymal stem cells (MSCs) for cell therapy. In the pathology of RA, the pro-inflammator...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-012-9354-9
更新日期:2013-12-01 00:00:00
abstract::Autologous chondrocyte implantation has been shown to be a promising method for treatment of deep articular cartilage defects. The hyaline cartilage formed by implanted autologous chondrocytes has biomechanical properties similar to those of natural articular cartilage. Between June 2006 and September 2008 we performe...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-009-9163-y
更新日期:2010-05-01 00:00:00
abstract::Terminal sterilization of musculoskeletal allografts by gamma radiation minimizes the risk of disease transmission but impairs allograft mechanical properties. Commonly employed crosslinking agents can sterilize tissues without affecting mechanical properties adversely; however, these agents are toxic. Genipin is repo...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-012-9335-z
更新日期:2013-09-01 00:00:00
abstract::A tympano-ossicular tissue bank complying with European Union regulations on human allografts is feasible and critical to assure that the patient receives tissue which is safe, individually checked and prepared in a suitable environment. The transcranial procurement technique has become the standard approach to procur...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-012-9344-y
更新日期:2013-09-01 00:00:00
abstract::Human adipose-derived stem cells (hADSCs) have the ability to influence immune response, and hence are key cell sources for tissue repair and regeneration. In this study we explored the effect of continuous passage on the immunomodulatory properties of hADSCs to provide some advises for large-scale production of hADSC...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-014-9451-z
更新日期:2015-03-01 00:00:00
abstract::Cerebral ischemic injury as the main manifestation of stroke can occur in stroke patients (70-80%). Nowadays, the main therapeutic strategy used for ischemic brain injury treatment aims to achieve reperfusion, neuroprotection, and neurorecovery. Also, angiogenesis as a therapeutic approach maybe represents a promising...
journal_title:Cell and tissue banking
pub_type: 杂志文章,评审
doi:10.1007/s10561-020-09885-6
更新日期:2020-11-24 00:00:00
abstract::Human amniotic membrane (HAM) is used as an allograft in regenerative medicine or as a source of pluripotent cells for stem cell research. Various decontamination protocols and solutions are used to sterilize HAM before its application, but little is known about the toxicity of disinfectants on HAM cells. In this stud...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-017-9636-3
更新日期:2017-09-01 00:00:00
abstract::Tissue banking is started in Thailand in 1979 at the Department of Orthopaedic Surgery, Siriraj Hospital, Bangkok. At that time tissues produced were freeze-dried bone allografts which were sterilized by ethylene oxide. In 1984, the freeze-dried tissue allograft project received an award from the National Research Cou...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/A:1026542730541
更新日期:2000-01-01 00:00:00
abstract::Defatting is an important procedure for the preparation of bone grafts because lipids in bone grafts strongly influence the osteointegration. Lipases have been widely used in different fields. However, study on the application to defatting process for bone grafts preparation has never been found so far. In this study,...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-013-9391-z
更新日期:2014-09-01 00:00:00
abstract::Corneal disease is the second cause of blindness in developing countries, where the number of corneal grafts needed by far exceeds the number available. In industrialized countries, although corneas are generally available for keratoplasty, onto inflamed and vascularized host beds they are often rejected despite immun...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-018-9742-x
更新日期:2019-03-01 00:00:00
abstract::Cryopreserved (CryoPA) and Glycerol-preserved (GPA) skin allografts are commonly used in the treatment of severe burn injuries. However, comparable data on their differences in clinical outcome is scarce. This retrospective review aims to study the effect of allograft viability on clinical outcomes. The records of 48 ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-011-9254-4
更新日期:2012-06-01 00:00:00
abstract::Although therapeutics derived from biological sources have been subjected to regulatory oversight for some time, the products used in transplantation procedures have historically been exempt from this oversight. These products have been viewed as being part of medical practice rather than as the result of mainstream p...
journal_title:Cell and tissue banking
pub_type: 杂志文章,评审
doi:10.1007/s10561-006-9012-1
更新日期:2006-01-01 00:00:00
abstract::Perivascular cells are known to be ancestors of mesenchymal stem cells (MSCs) and can be obtained from heart, skin, bone marrow, eye, placenta and umbilical cord (UC). However detailed characterization of perivascular cells around the human UC vein and comparative analysis of them with MSCs haven't been done yet. In t...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-015-9542-5
更新日期:2016-06-01 00:00:00
abstract::To vitrify human amniotic membrane specimens so that the maximum of epithelial cells survives in order to develop a procedure for the eventual vitrification of posterior corneal lamellae without using cryoprotective agents. To assess different methods of tissue sample preparation preceding vitrification. In group 1, t...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-013-9388-7
更新日期:2014-03-01 00:00:00
abstract::Current regulatory requirements demand an in-depth understanding and validation of protocols used in tissue banking. The aim of this work was to characterize the quality of split thickness skin allografts cryopreserved or manufactured using highly concentrated solutions of glycerol (50, 85 or 98%), where tissue water ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-018-9694-1
更新日期:2018-06-01 00:00:00
abstract::Islet transplantation has made major progress to treat patients with type 1 diabetes. Islet mass and quality are critically important to ensure successful transplantation. Currently, islet status is evaluated using insulin secretion, oxygen consumption rate, or adenosine triphosphate (ATP) measurement. These parameter...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-017-9659-9
更新日期:2018-03-01 00:00:00
abstract::Our Department of Plastic and Reconstructive Surgery has routinely been using amnion preserved in glycerol for the treatment of debrided II degrees burns. This treatment is almost pain free and requires fewer changes of dressings and fewer anaesthetics. It also prevents overgrowing granulation tissue and lessens scarr...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1023/A:1014316232009
更新日期:2001-01-01 00:00:00
abstract::This study was conducted to evaluate the effect of combined cysteine and glutathione in soy lecithin-based semen extender on post-thawed ram sperm quality. A total of 28 ejaculates were collected twice a week (from four rams) during breeding season. In each replicate, semen samples (n = 4, one ejaculate for each ram) ...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-014-9488-z
更新日期:2015-09-01 00:00:00
abstract::We evaluated the value of the 'alternative slices mirror image method' used in prostate tissue banking in terms of predicting the sampling of cancerous tissue while preserving the pathological prognostic information. The concordance of diagnosis between banked sections and their mirror image paraffin- sections was stu...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-011-9284-y
更新日期:2012-12-01 00:00:00
abstract::Blood samples collected from deceased tissue donors for mandatory transfusion microbiology testing may be taken either at the time of tissue donation, or residual samples may be retrieved from hospital laboratories where they were originally used for ante-mortem tests. In the latter case, sample labelling may not conf...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-008-9071-6
更新日期:2008-12-01 00:00:00
abstract::There are controversial reports about cardiac differentiation potential of mesenchymal stem cells (MSCs), and there is still no well-defined protocol for the induction of cardiac differentiation. The effects of retinoic acid (RA) and dimethyl sulfoxide (DMSO) on the proliferation and differentiation of human fetal liv...
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-015-9514-9
更新日期:2016-03-01 00:00:00
abstract::Human skin allografts can be preserved by different methods. In our clinical practice, human skin allografts are harvested on multi-organ and tissue donors, transferred at +4°C in Ringer Lactate, cryopreserved with 15% Glycerol and held in the vapor phase of a liquid nitrogen freezer until delivery to the burn center....
journal_title:Cell and tissue banking
pub_type: 杂志文章
doi:10.1007/s10561-011-9239-3
更新日期:2012-03-01 00:00:00