Isolation of human dermis derived mesenchymal stem cells using explants culture method: expansion and phenotypical characterization.

Abstract:

:Recent studies have reported that stem cells can be isolated from a wide range of tissues including bone marrow, fatty tissue, adipose tissue and placenta. Moreover, several studies also suggest that skin dermis could serve as a source of stem cells, but are of unclear phenotype. Therefore, we isolated and investigated to determine the potential of stem cell within human skin dermis. We isolated cells from human dermis, termed here as human dermis-derived mesenchymal stem cells (hDMSCs) which is able to be isolated by using explants culture method. Our method has an advantage over the enzymatic method as it is easier, less expensive and less cell damage. hDMSCs were maintained in basal culture media and proliferation potential was measured. hDMSCs were highly proliferative and successfully expanded with no additional growth factor. In addition, hDMSCs revealed normal karyotype and expressed high levels of CD90, CD73 and CD105 while did not express the surface markers for CD34, CD45 and HLA-DR. Also, we confirmed that hDMSCs possess the capacity to differentiate into multiple lineage including adipocyte, osteocyte, chondrocyte and precursor of hepatocyte lineage. Considering these results, we suggest that hDMSCs might be a valuable source of stem cells and could potentially be a useful source of clinical application.

journal_name

Cell Tissue Bank

journal_title

Cell and tissue banking

authors

Park JR,Kim E,Yang J,Lee H,Hong SH,Woo HM,Park SM,Na S,Yang SR

doi

10.1007/s10561-014-9471-8

subject

Has Abstract

pub_date

2015-06-01 00:00:00

pages

209-18

issue

2

eissn

1389-9333

issn

1573-6814

journal_volume

16

pub_type

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