Abstract：
:The contamination of the transport solution used in cardiovascular allografts can occur from different sources. Risk factors associated with positive microbiological test of transport solution have not been reported previously. This study aimed to determine the risk factor for contamination of transport solution used in the heart valve allografts stored in a Brazilian tissue bank. This retrospective study was conducted on all donors of cardiovascular allografts stored in a tissue bank from December 2008 to December 2017. Microbiological cultures for aerobic and anaerobic bacteria, fungi/yeasts were carried out in TS. Clinical variables were included. From 1001 transport solution, 52% were contaminated. A total of 770 microorganisms were identified, and Staphylococcus spp. was identified in 248 isolates (32.2%). Skin bacteria from skin microbiota were the most commonly identified microorganisms (Staphylococcus spp., Cutibacterium spp., Corynebacterium spp., and Bacillus spp.), occurring in 49.6%. The presence of a diagnosis of healthcare-associated infection was not associated with skin contamination (odds ratio [OR] 0.62 [0.41-0.94]; p = 0.014). Conditions like fever, use of antibiotics, and leukocytosis were less likely associated with contamination of transport solution. A longer warm ischemic time was associated with higher frequency of contamination. In the multivariable analysis, warm ischemic time was independently associated with contamination, and antibiotic therapy was a factor that decreased the rate of contamination (p < 0.05). Contamination of transport solution is associated with modifiable risk factors, such as warm ischemic time. Measures to minimize contamination should be employed to avoid unnecessary tissue discharges.
journal_name
Cell Tissue Bankjournal_title
Cell and tissue bankingauthors
Sawa B,Ribeiro VST,Kraft L,Wollmann LC,Pegoraro D,Suss PH,Tuon FFdoi
10.1007/s10561-019-09754-xsubject
Has Abstractpub_date
2019-03-01 00:00:00pages
87-94issue
1eissn
1389-9333issn
1573-6814pii
10.1007/s10561-019-09754-xjournal_volume
20pub_type
杂志文章abstract：:Human sperm cryopreservation is a common technique which is used in assisted reproductive technologies. Despite the existence of evidence supporting the production of ROS and DNA fragmentation during sperm cryopreservation, there is little and equivocal information about the cryopreservation effects on methylation of ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09828-1
更新日期：2020-09-01 00:00:00
abstract：:This is a report of a workshop held on the establishment of human research tissue banking which was held in Levi, Finland 21-24 March 2002. There were 21 participants from 7 European countries. This meeting was attended by representatives from academia, research tissue banks and from the Biotech and Pharmaceutical Ind...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1022811232250
更新日期：2002-01-01 00:00:00
abstract：:Freeze-dried dura mater allograft (FDDMA) was evaluated as a cost-effective collagenous barrier using an experimental animal model to determine its biocompatibility and 'cell-occlusivity' potential. The FDDMA was processed at the Civil Hospital Ahmedabad, in accordance with the guidelines provided by the South-Eastern...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/B:CATB.0000007039.98845.bd
更新日期：2003-01-01 00:00:00
abstract：:The amniotic membranes were collected from the placentae of selected and screened donors. Processing was done by washing the fresh amniotic membrane successively in sterile saline, 0.05% sodium hypochlorite solution and sterile distilled water until it was completely cleared of blood particles. The membranes were ster...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/B:CATB.0000034077.05000.29
更新日期：2004-01-01 00:00:00
abstract：:Perivascular cells are known to be ancestors of mesenchymal stem cells (MSCs) and can be obtained from heart, skin, bone marrow, eye, placenta and umbilical cord (UC). However detailed characterization of perivascular cells around the human UC vein and comparative analysis of them with MSCs haven't been done yet. In t...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-015-9542-5
更新日期：2016-06-01 00:00:00
abstract：:Recovered human and animal tissues are used extensively in surgery for wound repair and reconstruction. In preparation for the validation of chemical disinfection and radiation sterilization processes, studies were performed on the development and validation of quantitative bioburden recovery methods for human bone an...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-010-9238-9
更新日期：2012-03-01 00:00:00
abstract：:This study was conducted to evaluate the effect of combined cysteine and glutathione in soy lecithin-based semen extender on post-thawed ram sperm quality. A total of 28 ejaculates were collected twice a week (from four rams) during breeding season. In each replicate, semen samples (n = 4, one ejaculate for each ram) ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9488-z
更新日期：2015-09-01 00:00:00
abstract：:Both allogenic and autologous cultured skin cells have been used clinically on burn patients. In vitro cultivation of human keratinocytes has been routinely provided by the Central Tissue Bank in Bratislava since 1996, with an average annual production of around 7,000 cm(2). Keratinocytes have been cultivated using a ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9275-z
更新日期：2012-08-01 00:00:00
abstract：:The amnionic membrane is a rich source of multipotent mesenchymal stromal cells (hAMSC), which are readily available and show a potential use in regenerative medicine and tissue engineering. Before these cells can be applied clinically, careful characterization is necessary, especially as primary cells are known to ch...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-013-9415-8
更新日期：2014-06-01 00:00:00
abstract：:Stem cell therapy is in constant need of new cell sources to conceive regenerative medicine approaches for diseases that are still without therapy. Scientists drew the attention toward amniotic membrane and amniotic fluid stem cells, since these sources possess many advantages: first of all as cells can be extracted f...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-014-9428-y
更新日期：2014-06-01 00:00:00
abstract：:In breast cancer, various clinical parameters are assessed to define clinical stage and thus obtain a more accurate prognosis. However, banks of tumor tissues are an important source of material for studies of risk of recurrence and of features governing clinical outcome in breast cancer. Although the heterogeneous ch...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-009-9124-5
更新日期：2009-11-01 00:00:00
abstract：:Autologous chondrocyte implantation has been shown to be a promising method for treatment of deep articular cartilage defects. The hyaline cartilage formed by implanted autologous chondrocytes has biomechanical properties similar to those of natural articular cartilage. Between June 2006 and September 2008 we performe...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-009-9163-y
更新日期：2010-05-01 00:00:00
abstract：:On March 19 World Health Organization declare the pandemic situation by outbreak coronavirus disease 2019 in the world. The pressure on the health care system has been very high in several countries. Spanish National Transplant Organization (ONT) have made many efforts in maintaining transplantation activity. Although...
journal_title：Cell and tissue banking
pub_type： 杂志文章,多中心研究
doi：10.1007/s10561-020-09853-0
更新日期：2020-12-01 00:00:00
abstract：:During bone allograft processing, despite stringent donor screening and use of aseptic techniques, microbial invasion may occur due to the porous nature of the graft and cause potentially fatal infections. The aim of the present study was to prepare bone allograft with and without gentamicin and to compare bioburden a...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-019-09763-w
更新日期：2019-06-01 00:00:00
abstract：:Cellular stress to ejaculated spermatozoa such as cryopreservation is known to induce caspase-derived, apoptotic signaling. Therefore, the proenzymes and active forms of caspases 1, 3, 8 and 9 were examined by western blot technique in unfrozen and frozen human spermatozoa of infertility patients and of healthy donors...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-005-0276-7
更新日期：2006-01-01 00:00:00
abstract：:Current regulatory requirements demand an in-depth understanding and validation of protocols used in tissue banking. The aim of this work was to characterize the quality of split thickness skin allografts cryopreserved or manufactured using highly concentrated solutions of glycerol (50, 85 or 98%), where tissue water ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-018-9694-1
更新日期：2018-06-01 00:00:00
abstract：:Blood samples collected from deceased tissue donors for mandatory transfusion microbiology testing may be taken either at the time of tissue donation, or residual samples may be retrieved from hospital laboratories where they were originally used for ante-mortem tests. In the latter case, sample labelling may not conf...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-008-9071-6
更新日期：2008-12-01 00:00:00
abstract：:Terminal sterilization of musculoskeletal allografts by gamma radiation minimizes the risk of disease transmission but impairs allograft mechanical properties. Commonly employed crosslinking agents can sterilize tissues without affecting mechanical properties adversely; however, these agents are toxic. Genipin is repo...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-012-9335-z
更新日期：2013-09-01 00:00:00
abstract：:The Israel National Skin Bank (INSB) was founded jointly by the Israel Defense Forces (IDF) Medical Corps and the Ministry of Health in 1986. The prime purpose of the Skin Bank is to treat burn victims incurred at war or during mass casualty incidences. The INSB Protocol is comprised of international skin bank protoco...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1010183719662
更新日期：2000-01-01 00:00:00
abstract：:The purpose of the present research was: 1. To investigate the opportunity of application of allogen osteoplastic materials such as Lioplast, received in Samara Tissue Bank of Samara State Medical University. 2. To work out a new technique of producing lyophilized allogen osteoplastic materials, such as Lioplast, with...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-004-7254-3
更新日期：2005-01-01 00:00:00
abstract：:Quality control and standardized preservation methods are essential in the field of transplantation. The International Organization for Standardization (ISO) has established a common set of manufacturing, trade and communications standards that are applicable worldwide and that provide the basis of a quality plan for ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1011576332362
更新日期：2001-01-01 00:00:00
abstract：:Wound healing is a complex process that involves interaction of soluble mediators, extracellular matrix and infiltrating blood cells. Chronic and non-healing skin defects contribute significantly to morbidity and mortality of many patients. Recently, despite the current medical progress, the chronic and non-healing wo...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-014-9440-2
更新日期：2015-03-01 00:00:00
abstract：:It has been predicted that one of the greatest increase in prevalence of diabetes will happen in the Middle East bear in the next decades. The aim of standard therapeutic strategies for diabetes is better control of complications. In contrast, some new strategies like cell and gene therapy have aimed to cure the disea...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9273-1
更新日期：2012-12-01 00:00:00
abstract：:During August and September of 2013, temperature data loggers were shipped to and from an AATB accredited and FDA registered allograft tissue processing facility in Belgrade, MT (Bacterin International, Inc.) to five warm climate cities (Dallas, TX, El Paso, TX, New Orleans, LA, Phoenix, AZ, and Tampa, FL). Shipping d...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-016-9578-1
更新日期：2016-12-01 00:00:00
abstract：:Human skin allografts are one of the best temporary biological coverings for severely burned patients. Cryopreserved (CPA) and glycerol-preserved (GPA) allografts are the most widely used types. This study compared the allograft efficiency of both preservation methods under the same conditions. To simulate actual clin...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-015-9521-x
更新日期：2016-03-01 00:00:00
abstract：:To vitrify human amniotic membrane specimens so that the maximum of epithelial cells survives in order to develop a procedure for the eventual vitrification of posterior corneal lamellae without using cryoprotective agents. To assess different methods of tissue sample preparation preceding vitrification. In group 1, t...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-013-9388-7
更新日期：2014-03-01 00:00:00
abstract：:Rheumatoid arthritis (RA) is the most common degenerative arthritic cartilage and represents a disease where the prospect of stem cell therapy offers considerable hope. Currently, bone marrow (BM) represents the major source of mesenchymal stem cells (MSCs) for cell therapy. In the pathology of RA, the pro-inflammator...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-012-9354-9
更新日期：2013-12-01 00:00:00
abstract：:Despite a plethora of studies on overall attitudes related to organ donation in a health care setting, little is known as to whether or not medical students at various levels of undergraduate training have distinctive attitudes towards donation. The purpose of this study was to analyze attitudes of first- and sixth-ye...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9491-4
更新日期：2015-09-01 00:00:00
abstract：:The present experiment was conducted to evaluate the effect of kinetin on ram semen quality during cold storage. Ejaculates were collected using an artificial vagina from five Qezel rams. Ejaculates which met the criteria (volume of 0.75-2 ml; minimum spermatozoa concentration of 2.5 × 109 spermatozoa/ml and forward p...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-016-9604-3
更新日期：2018-03-01 00:00:00
abstract：:In order to guarantee the required level of quality for our Bone &Tissue Banking, we evaluated a new CE marked container (CELLFLEX MacoPharma), for packing, transport, processing and storage of bones for therapeutic use. The use of CE marked containers is mandatory for organ and tissue containers (Medical Device Direc...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-004-1090-3
更新日期：2004-01-01 00:00:00
