Abstract：
:The aim of the present study was to compare the effects of two freezing methods, vapor phase and very rapid freezing, with and without cryoprotectant on semen parameters in men with normal semen criteria. Cryopreservation was done on semen samples from 31 men by two methods of vapor phase freezing and very rapid freezing, with and without Test Yolk buffered glycerol (TYBG) as cryoprotectant. The motility, viability, acrosome and DNA integrity were evaluated on fresh and post-thaw samples. Post-thaw sperm progressive motility was significantly higher in the presence of TYBG in the vapor phase cryopreservation (%6.30 ± 3.74) compared with the very rapid freezing method (%2.2 ± 1.97 and %4.00 ± 2.42 in the presence and absence of TYBG, respectively). There was no significant difference in viability, acrosome status and DNA integrity between two methods in presence or absence of TYBG. The very rapid freezing method in the absence of TYBG showed better sperm motility but viability, acrosome and DNA integrity were similar to the presence of TYBG. The results show that cryopreservation of human spermatozoa together with seminal plasma by using vapor phase method is better than very rapid freezing method to preserve sperm progressive motility; however very rapid freezing method is quick and simple and do not require special cryoprotectant. It can be used for cryopreservation of small number of spermatozoa in IVF centers.
journal_name
Cell Tissue Bankjournal_title
Cell and tissue bankingauthors
Darvishnia H,Lakpour N,Lahijani MS,Heidari-Vala H,Akhondi MA,Zeraati H,Sadeghi MRdoi
10.1007/s10561-012-9351-zsubject
Has Abstractpub_date
2013-12-01 00:00:00pages
679-85issue
4eissn
1389-9333issn
1573-6814journal_volume
14pub_type
杂志文章abstract：:Mesenchymal stem cells (MSCs) are used for tissue regeneration in several pathological conditions, including autoimmune diseases. However, the optimal sources and culture requirements for these cells are still under investigation. Here, we compared mRNA expression in dermal MSCs (DMSCs) at passage (P) 3 and P5 to prov...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-017-9672-z
更新日期：2018-09-01 00:00:00
abstract：:Human adipose-derived stem cells (hADSCs) have the ability to influence immune response, and hence are key cell sources for tissue repair and regeneration. In this study we explored the effect of continuous passage on the immunomodulatory properties of hADSCs to provide some advises for large-scale production of hADSC...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9451-z
更新日期：2015-03-01 00:00:00
abstract：:In order to guarantee the required level of quality for our Bone &Tissue Banking, we evaluated a new CE marked container (CELLFLEX MacoPharma), for packing, transport, processing and storage of bones for therapeutic use. The use of CE marked containers is mandatory for organ and tissue containers (Medical Device Direc...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-004-1090-3
更新日期：2004-01-01 00:00:00
abstract：:Osteochondral defects can degenerate into osteoarthritis and currently there are no good treatment alternatives available to most Orthopaedic surgeons. Osteochondral allografting can restore damaged joint surfaces but its clinical use is limited by poor access to high quality tissue. Vitrification of osteochondral tis...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-006-9023-y
更新日期：2007-01-01 00:00:00
abstract：:To vitrify human amniotic membrane specimens so that the maximum of epithelial cells survives in order to develop a procedure for the eventual vitrification of posterior corneal lamellae without using cryoprotective agents. To assess different methods of tissue sample preparation preceding vitrification. In group 1, t...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-013-9388-7
更新日期：2014-03-01 00:00:00
abstract：:Perivascular cells are known to be ancestors of mesenchymal stem cells (MSCs) and can be obtained from heart, skin, bone marrow, eye, placenta and umbilical cord (UC). However detailed characterization of perivascular cells around the human UC vein and comparative analysis of them with MSCs haven't been done yet. In t...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-015-9542-5
更新日期：2016-06-01 00:00:00
abstract：:The use of glycosaminoglycans (GAGs) as the second macromolecular component with collagen for the membrane preparation is based on the idea to create a biodegradable scaffold for new tissue. A hybrid collagen/hyaluronan membrane with specific bubble macrostructure was designed to serve as a synthetic dermal substitute...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/a:1010181925236
更新日期：2000-01-01 00:00:00
abstract：:Human sperm banking is an important procedure in the assisted reproductive technique centers. It entails sperm damage. The aim of this study was to investigate beneficial effect of Ceratonia siliqua (C. siliqua) supplement in freezing/thawing media on post thaw sperm parameters and sperm chromatin quality in normozoos...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-019-09779-2
更新日期：2019-09-01 00:00:00
abstract：:During August and September of 2013, temperature data loggers were shipped to and from an AATB accredited and FDA registered allograft tissue processing facility in Belgrade, MT (Bacterin International, Inc.) to five warm climate cities (Dallas, TX, El Paso, TX, New Orleans, LA, Phoenix, AZ, and Tampa, FL). Shipping d...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-016-9578-1
更新日期：2016-12-01 00:00:00
abstract：:Decellularised tissue allografts have been used in reconstructive surgical applications and transplantation for many years. Some of the current methods of sterilisation have a detrimental effect on the tissue graft structure and function. The anti-microbial activity of cupric ions and hydrogen peroxide (H2O2) are well...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-017-9660-3
更新日期：2017-12-01 00:00:00
abstract：:This study was conducted to evaluate the effect of combined cysteine and glutathione in soy lecithin-based semen extender on post-thawed ram sperm quality. A total of 28 ejaculates were collected twice a week (from four rams) during breeding season. In each replicate, semen samples (n = 4, one ejaculate for each ram) ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9488-z
更新日期：2015-09-01 00:00:00
abstract：:Terminal sterilization of musculoskeletal allografts by gamma radiation minimizes the risk of disease transmission but impairs allograft mechanical properties. Commonly employed crosslinking agents can sterilize tissues without affecting mechanical properties adversely; however, these agents are toxic. Genipin is repo...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-012-9335-z
更新日期：2013-09-01 00:00:00
abstract：:Freeze-dried dura mater allograft (FDDMA) was evaluated as a cost-effective collagenous barrier using an experimental animal model to determine its biocompatibility and 'cell-occlusivity' potential. The FDDMA was processed at the Civil Hospital Ahmedabad, in accordance with the guidelines provided by the South-Eastern...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/B:CATB.0000007039.98845.bd
更新日期：2003-01-01 00:00:00
abstract：:Stem cell therapy is in constant need of new cell sources to conceive regenerative medicine approaches for diseases that are still without therapy. Scientists drew the attention toward amniotic membrane and amniotic fluid stem cells, since these sources possess many advantages: first of all as cells can be extracted f...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-014-9428-y
更新日期：2014-06-01 00:00:00
abstract：:Corneal disease is the second cause of blindness in developing countries, where the number of corneal grafts needed by far exceeds the number available. In industrialized countries, although corneas are generally available for keratoplasty, onto inflamed and vascularized host beds they are often rejected despite immun...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-018-9742-x
更新日期：2019-03-01 00:00:00
abstract：:Although allografts for anterior cruciate ligament (ACL) replacement have shown advantages compared to autografts, their use is limited due to the risk of disease transmission and the limitations of available sterilization methods. Gamma sterilization has shown detrimental effects on graft properties at the high doses...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9289-6
更新日期：2012-08-01 00:00:00
abstract：:Current regulatory requirements demand an in-depth understanding and validation of protocols used in tissue banking. The aim of this work was to characterize the quality of split thickness skin allografts cryopreserved or manufactured using highly concentrated solutions of glycerol (50, 85 or 98%), where tissue water ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-018-9694-1
更新日期：2018-06-01 00:00:00
abstract：:The contamination of the transport solution used in cardiovascular allografts can occur from different sources. Risk factors associated with positive microbiological test of transport solution have not been reported previously. This study aimed to determine the risk factor for contamination of transport solution used ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-019-09754-x
更新日期：2019-03-01 00:00:00
abstract：:Human sperm cryopreservation is a common technique which is used in assisted reproductive technologies. Despite the existence of evidence supporting the production of ROS and DNA fragmentation during sperm cryopreservation, there is little and equivocal information about the cryopreservation effects on methylation of ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09828-1
更新日期：2020-09-01 00:00:00
abstract：:There are controversial reports about cardiac differentiation potential of mesenchymal stem cells (MSCs), and there is still no well-defined protocol for the induction of cardiac differentiation. The effects of retinoic acid (RA) and dimethyl sulfoxide (DMSO) on the proliferation and differentiation of human fetal liv...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-015-9514-9
更新日期：2016-03-01 00:00:00
abstract：:During bone allograft processing, despite stringent donor screening and use of aseptic techniques, microbial invasion may occur due to the porous nature of the graft and cause potentially fatal infections. The aim of the present study was to prepare bone allograft with and without gentamicin and to compare bioburden a...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-019-09763-w
更新日期：2019-06-01 00:00:00
abstract：:The purpose of the present research was: 1. To investigate the opportunity of application of allogen osteoplastic materials such as Lioplast, received in Samara Tissue Bank of Samara State Medical University. 2. To work out a new technique of producing lyophilized allogen osteoplastic materials, such as Lioplast, with...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-004-7254-3
更新日期：2005-01-01 00:00:00
abstract：:Outcomes for patients with metastatic and recurrent osteosarcoma remain poor and a better understanding of the biology of this malignancy is critical to the development of prognostic biomarkers and novel therapies. The purpose of this study was to establish a biobank of osteosarcoma which has the potential of monitori...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09831-6
更新日期：2020-09-01 00:00:00
abstract：:Human epidermal melanocytes can be induced to form melanocyte spheroids and revert to immature characteristics by long-term trypsinization (LTT). To further explore the biological characteristics of melanocytes after LTT and to study the underlying mechanism. Melanocytes were subjected to long-term (2 h) trypsinizatio...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09866-9
更新日期：2020-09-25 00:00:00
abstract：:The present experiment was conducted to evaluate the effect of kinetin on ram semen quality during cold storage. Ejaculates were collected using an artificial vagina from five Qezel rams. Ejaculates which met the criteria (volume of 0.75-2 ml; minimum spermatozoa concentration of 2.5 × 109 spermatozoa/ml and forward p...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-016-9604-3
更新日期：2018-03-01 00:00:00
abstract：:Human skin allografts are one of the best temporary biological coverings for severely burned patients. Cryopreserved (CPA) and glycerol-preserved (GPA) allografts are the most widely used types. This study compared the allograft efficiency of both preservation methods under the same conditions. To simulate actual clin...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-015-9521-x
更新日期：2016-03-01 00:00:00
abstract：:Human skin allografts can be preserved by different methods. In our clinical practice, human skin allografts are harvested on multi-organ and tissue donors, transferred at +4°C in Ringer Lactate, cryopreserved with 15% Glycerol and held in the vapor phase of a liquid nitrogen freezer until delivery to the burn center....
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9239-3
更新日期：2012-03-01 00:00:00
abstract：:Islet transplantation has made major progress to treat patients with type 1 diabetes. Islet mass and quality are critically important to ensure successful transplantation. Currently, islet status is evaluated using insulin secretion, oxygen consumption rate, or adenosine triphosphate (ATP) measurement. These parameter...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-017-9659-9
更新日期：2018-03-01 00:00:00
abstract：:To analyze the data of the adverse events collected in a single major eye bank (EFS Bourgogne Franche Comté, Besançon, France) for the year 2013 and to report the French data of biovigilance provided by the French National Agency for Medicines and Health Products Safety (ANSM) between 2010 and 2013. we have set up a s...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-016-9593-2
更新日期：2017-03-01 00:00:00
abstract：:We evaluated the value of the 'alternative slices mirror image method' used in prostate tissue banking in terms of predicting the sampling of cancerous tissue while preserving the pathological prognostic information. The concordance of diagnosis between banked sections and their mirror image paraffin- sections was stu...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9284-y
更新日期：2012-12-01 00:00:00
