Abstract：
:Decellularised tissue allografts have been used in reconstructive surgical applications and transplantation for many years. Some of the current methods of sterilisation have a detrimental effect on the tissue graft structure and function. The anti-microbial activity of cupric ions and hydrogen peroxide (H2O2) are well known however their combined application is not currently utilised as a decontamination agent in the tissue banking world sector. The aim of this study was to determine the combined concentrations of copper chloride (CuCl2) and H2O2 that have the optimal bactericidal and sporicidal activity on decellularised (dCELL) human dermis. The first part of this study established the decimal reduction time (D-value) of CuCl2 (0.1 mg/L and 1 mg/L) together with H2O2 (0.01, 0.1, 0.5 and 1%) for Staphylococcus epidermidis, Escherichia coli and Bacillus subtilis spores. The second part of this study identified the most effective CuCl2 and H2O2 concentration that decontaminated dCELL human dermis inoculated with these pathogens. Of all the concentrations tested, 0.1 mg/L CuCl2 in combination with 1% H2O2 had the shortest D-value; S. epidermidis D = 3.15 min, E. coli D = 2.62 min and B. subtilis spores D = 18.05 min. However when adsorbed onto dCELL dermis, S. epidermidis and E. coli were more susceptible to 1 mg/L CuCl2 together with 0.5% H2O2. These studies show promise of CuCl2-H2O2 formulations as potential sterilants for decellularised dermal allografts.
journal_name
Cell Tissue Bankjournal_title
Cell and tissue bankingauthors
Leow-Dyke SF,Rooney P,Kearney JNdoi
10.1007/s10561-017-9660-3subject
Has Abstractpub_date
2017-12-01 00:00:00pages
561-572issue
4eissn
1389-9333issn
1573-6814pii
10.1007/s10561-017-9660-3journal_volume
18pub_type
杂志文章abstract：:Tissue engineering using human cells and tissue has one of the greatest scientific and economical potential in the coming years. There are public concerns during the ongoing discussion about future trends in life sciences and if ethic boundaries might be respected sufficiently in the course of striving for industrial ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/A:1026392429112
更新日期：2003-01-01 00:00:00
abstract：:The aim of the study was to determine the relationship between the maternal age at delivery and selected properties of the cord blood stem cells. The study included 50 pregnant women aged between 18 and 38 years in which spontaneous labors or elective cesarean sections were performed. Umbilical cord blood was collecte...
journal_title：Cell and tissue banking
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更新日期：2018-06-01 00:00:00
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journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-019-09763-w
更新日期：2019-06-01 00:00:00
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journal_title：Cell and tissue banking
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doi：10.1007/s10561-020-09856-x
更新日期：2020-12-01 00:00:00
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journal_title：Cell and tissue banking
pub_type： 杂志文章
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journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9488-z
更新日期：2015-09-01 00:00:00
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journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9463-8
更新日期：2015-06-01 00:00:00
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journal_title：Cell and tissue banking
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更新日期：2006-01-01 00:00:00
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journal_title：Cell and tissue banking
pub_type： 杂志文章
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更新日期：2008-12-01 00:00:00
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journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-018-09744-5
更新日期：2019-02-11 00:00:00
abstract：:This study aimed to optimise methods of cryopreserving human articular cartilage (AC) tissue for the isolation of late chondrocytes. Human AC specimens from osteoarthritis patients who had undergone total knee replacement were used to optimise the chondrocyte isolation process and the choice of cryoprotective agent (C...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-012-9328-y
更新日期：2013-06-01 00:00:00
abstract：:Current regulatory requirements demand an in-depth understanding and validation of protocols used in tissue banking. The aim of this work was to characterize the quality of split thickness skin allografts cryopreserved or manufactured using highly concentrated solutions of glycerol (50, 85 or 98%), where tissue water ...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-018-9694-1
更新日期：2018-06-01 00:00:00
abstract：:The aim of this study is to set up a standardized and reproducible method to determine the potency (= stem cell content) of human conjunctival cell cultures by means of immunofluorescence-based analyses. This will help the development of new Advanced Therapy Medicinal Products (ATMPs) to use in future cell therapy cli...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09874-9
更新日期：2020-10-13 00:00:00
abstract：:We evaluated the value of the 'alternative slices mirror image method' used in prostate tissue banking in terms of predicting the sampling of cancerous tissue while preserving the pathological prognostic information. The concordance of diagnosis between banked sections and their mirror image paraffin- sections was stu...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-011-9284-y
更新日期：2012-12-01 00:00:00
abstract：:Terminal sterilization of musculoskeletal allografts by gamma radiation minimizes the risk of disease transmission but impairs allograft mechanical properties. Commonly employed crosslinking agents can sterilize tissues without affecting mechanical properties adversely; however, these agents are toxic. Genipin is repo...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-012-9335-z
更新日期：2013-09-01 00:00:00
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journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09819-2
更新日期：2020-06-01 00:00:00
abstract：:Recent studies have reported that stem cells can be isolated from a wide range of tissues including bone marrow, fatty tissue, adipose tissue and placenta. Moreover, several studies also suggest that skin dermis could serve as a source of stem cells, but are of unclear phenotype. Therefore, we isolated and investigate...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-014-9471-8
更新日期：2015-06-01 00:00:00
abstract：:Homograft or allograft tissue has been available for use as replacement for diseased valves or reconstruction of major vessels for decades. However, with respect to replacement of diseased valvular tissue the search for the ideal valve still continues. In this review we will discuss the clinical indications, surgical ...
journal_title：Cell and tissue banking
pub_type： 杂志文章,评审
doi：10.1007/s10561-006-9009-9
更新日期：2006-01-01 00:00:00
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journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-008-9078-z
更新日期：2008-09-01 00:00:00
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journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-007-9052-1
更新日期：2008-03-01 00:00:00
abstract：:Islet transplantation has made major progress to treat patients with type 1 diabetes. Islet mass and quality are critically important to ensure successful transplantation. Currently, islet status is evaluated using insulin secretion, oxygen consumption rate, or adenosine triphosphate (ATP) measurement. These parameter...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-017-9659-9
更新日期：2018-03-01 00:00:00
abstract：:Perivascular cells are known to be ancestors of mesenchymal stem cells (MSCs) and can be obtained from heart, skin, bone marrow, eye, placenta and umbilical cord (UC). However detailed characterization of perivascular cells around the human UC vein and comparative analysis of them with MSCs haven't been done yet. In t...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-015-9542-5
更新日期：2016-06-01 00:00:00
abstract：:The purpose of this study is to evaluate the reliability of cartilage digestion and fluorescein diacetate-ethidium bromide (FDA-EB) fluorescence staining for the detection of chondrocyte viability in osteochondral grafts. Sixteen fresh osteochondral grafts were harvested from pig knee condyles, and the articular carti...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-018-9685-2
更新日期：2018-09-01 00:00:00
abstract：:There are controversial reports about cardiac differentiation potential of mesenchymal stem cells (MSCs), and there is still no well-defined protocol for the induction of cardiac differentiation. The effects of retinoic acid (RA) and dimethyl sulfoxide (DMSO) on the proliferation and differentiation of human fetal liv...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-015-9514-9
更新日期：2016-03-01 00:00:00
abstract：:Outcomes for patients with metastatic and recurrent osteosarcoma remain poor and a better understanding of the biology of this malignancy is critical to the development of prognostic biomarkers and novel therapies. The purpose of this study was to establish a biobank of osteosarcoma which has the potential of monitori...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-020-09831-6
更新日期：2020-09-01 00:00:00
abstract：:Defatting is an important procedure for the preparation of bone grafts because lipids in bone grafts strongly influence the osteointegration. Lipases have been widely used in different fields. However, study on the application to defatting process for bone grafts preparation has never been found so far. In this study,...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1007/s10561-013-9391-z
更新日期：2014-09-01 00:00:00
abstract：:The use of glycosaminoglycans (GAGs) as the second macromolecular component with collagen for the membrane preparation is based on the idea to create a biodegradable scaffold for new tissue. A hybrid collagen/hyaluronan membrane with specific bubble macrostructure was designed to serve as a synthetic dermal substitute...
journal_title：Cell and tissue banking
pub_type： 杂志文章
doi：10.1023/a:1010181925236
更新日期：2000-01-01 00:00:00
abstract：:Increasingly dental surgeons face the challenge of reconstruction of the height and/or thickness of the alveolar ridge as more and more patients wish to have permanent restoration of their dental defects based on intraosseous implants. Evaluation of human allogeneic bone tissue grafts in reconstruction of atrophied al...
journal_title：Cell and tissue banking
pub_type： 临床试验,杂志文章
doi：10.1007/s10561-015-9512-y
更新日期：2015-12-01 00:00:00
abstract：BACKGROUND:Despite improved surgical techniques and the use of new medication, healing of corneal and conjunctival defects cannot always be achieved. In this connection the clinical use of human amnion, produced by different techniques, has represented a successful alternative for the past ten years. The purpose of the...
journal_title：Cell and tissue banking
pub_type： 临床试验,杂志文章
doi：10.1023/b:catb.0000022222.41304.de
更新日期：2004-01-01 00:00:00
