当前位置： SCI文献检索 > Nature Microbiology期刊下所有文献 > Comprehensive analysis of IncC plasmid conjugation identifies a crucial role for the transcriptional regulator AcaB.

Comprehensive analysis of IncC plasmid conjugation identifies a crucial role for the transcriptional regulator AcaB.

Abstract：

:The IncC family of broad-host-range plasmids enables the spread of antibiotic resistance genes among human enteric pathogens1-3. Although aspects of IncC plasmid conjugation have been well studied4-9, many roles of conjugation genes have been assigned based solely on sequence similarity. We applied hypersaturated transposon mutagenesis and transposon-directed insertion-site sequencing to determine the set of genes required for IncC conjugation. We identified 27 conjugation genes, comprising 19 that were previously identified (including two regulatory genes, acaDC) and eight not previously associated with conjugation. We show that one previously unknown gene, acaB, encodes a transcriptional regulator that has a crucial role in the regulation of IncC conjugation. AcaB binds upstream of the acaDC promoter to increase acaDC transcription; in turn, AcaDC activates the transcription of IncC conjugation genes. We solved the crystal structure of AcaB at 2.9-Å resolution and used this to guide functional analyses that reveal how AcaB binds to DNA. This improved understanding of IncC conjugation provides a basis for the development of new approaches to reduce the spread of these multi-drug-resistance plasmids.

journal_name

Nat Microbiol

journal_title

Nature microbiology

authors

Hancock SJ,Phan MD,Luo Z,Lo AW,Peters KM,Nhu NTK,Forde BM,Whitfield J,Yang J,Strugnell RA,Paterson DL,Walsh TR,Kobe B,Beatson SA,Schembri MA

doi

10.1038/s41564-020-0775-0

subject

Has Abstract

pub_date

2020-11-01 00:00:00

pages

1340-1348

issue

11

issn

2058-5276

pii

10.1038/s41564-020-0775-0

journal_volume

5

pub_type

杂志文章

相关文献

Nature Microbiology文献大全
  • Evolution of host adaptation in the Salmonella typhoid toxin.

    abstract：:The evolution of virulence traits is central for the emergence or re-emergence of microbial pathogens and for their adaptation to a specific host 1-5 . Typhoid toxin is an essential virulence factor of the human-adapted bacterial pathogen Salmonella Typhi 6,7 , the cause of typhoid fever in humans 8-12 . Typhoid toxin...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-017-0033-2

    authors： Gao X,Deng L,Stack G,Yu H,Chen X,Naito-Matsui Y,Varki A,Galán JE

    更新日期：2017-12-01 00:00:00

  • A Chlamydia effector combining deubiquitination and acetylation activities induces Golgi fragmentation.

    abstract：:Pathogenic bacteria are armed with potent effector proteins that subvert host signalling processes during infection1. The activities of bacterial effectors and their associated roles within the host cell are often poorly understood, particularly for Chlamydia trachomatis2, a World Health Organization designated neglec...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-018-0271-y

    authors： Pruneda JN,Bastidas RJ,Bertsoulaki E,Swatek KN,Santhanam B,Clague MJ,Valdivia RH,Urbé S,Komander D

    更新日期：2018-12-01 00:00:00

  • A natural toroidal microswimmer with a rotary eukaryotic flagellum.

    abstract：:We describe Idionectes vortex gen. nov., sp. nov., a unicellular microeukaryote that swims by continuous inversion of its surface, similar to a vortex ring. This previously unreported mode of motility approximates a hypothetical concept called the 'toroidal swimmer', in which a doughnut-shaped object rotates around it...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-019-0478-6

    authors： Hess S,Eme L,Roger AJ,Simpson AGB

    更新日期：2019-10-01 00:00:00

  • Immune priming and clearance of orally acquired RNA viruses in Drosophila.

    abstract：:Immune responses in insects are differentially triggered depending on the infection route used by the pathogen. In most studies involving Drosophila melanogaster and viruses, infection is done by injection, while oral infection, which is probably the most common route of viral entry in nature, remains unexplored. Here...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-018-0265-9

    authors： Mondotte JA,Gausson V,Frangeul L,Blanc H,Lambrechts L,Saleh MC

    更新日期：2018-12-01 00:00:00

  • Murine colitis reveals a disease-associated bacteriophage community.

    abstract：:The dysregulation of intestinal microbial communities is associated with inflammatory bowel diseases (IBD). Studies aimed at understanding the contribution of the microbiota to inflammatory diseases have primarily focused on bacteria, yet the intestine harbours a viral component dominated by prokaryotic viruses known ...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-018-0210-y

    authors： Duerkop BA,Kleiner M,Paez-Espino D,Zhu W,Bushnell B,Hassell B,Winter SE,Kyrpides NC,Hooper LV

    更新日期：2018-09-01 00:00:00

  • Structural dynamics of bacteriophage P22 infection initiation revealed by cryo-electron tomography.

    abstract：:For successful infection, bacteriophages must overcome multiple barriers to transport their genome and proteins across the bacterial cell envelope. We use cryo-electron tomography to study the infection initiation of phage P22 in Salmonella enterica serovar Typhimurium, revealing how a channel forms to allow genome tr...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-019-0403-z

    authors： Wang C,Tu J,Liu J,Molineux IJ

    更新日期：2019-06-01 00:00:00

  • Tagging the type VI secretion system.

    abstract：: ...

    journal_title：Nature microbiology

    pub_type： 评论,杂志文章

    doi：10.1038/s41564-018-0277-5

    authors： Francetic O

    更新日期：2018-11-01 00:00:00

  • The intestinal regionalization of acute norovirus infection is regulated by the microbiota via bile acid-mediated priming of type III interferon.

    abstract：:Evidence has accumulated to demonstrate that the intestinal microbiota enhances mammalian enteric virus infections1. For example, we and others previously reported that commensal bacteria stimulate acute and persistent murine norovirus infections2-4. However, in apparent contradiction of these results, the virulence o...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-019-0602-7

    authors： Grau KR,Zhu S,Peterson ST,Helm EW,Philip D,Phillips M,Hernandez A,Turula H,Frasse P,Graziano VR,Wilen CB,Wobus CE,Baldridge MT,Karst SM

    更新日期：2020-01-01 00:00:00

  • Author Correction: Evolution of host adaptation in the Salmonella typhoid toxin.

    abstract：:The original version of this Letter has been modified in the abstract and main text to better reflect the distribution of Neu5Ac sialoglycans in humans. Additionally, co-author Lingquan Deng's present address has been further clarified. ...

    journal_title：Nature microbiology

    pub_type： 已发布勘误

    doi：10.1038/s41564-017-0070-x

    authors： Gao X,Deng L,Stack G,Yu H,Chen X,Naito-Matsui Y,Varki A,Galán JE

    更新日期：2017-12-01 00:00:00

  • AXL promotes Zika virus infection in astrocytes by antagonizing type I interferon signalling.

    abstract：:Zika virus (ZIKV) is associated with neonatal microcephaly and Guillain-Barré syndrome1,2. While progress has been made in understanding the causal link between ZIKV infection and microcephaly3-9, the life cycle and pathogenesis of ZIKV are less well understood. In particular, there are conflicting reports on the role...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-017-0092-4

    authors： Chen J,Yang YF,Yang Y,Zou P,Chen J,He Y,Shui SL,Cui YR,Bai R,Liang YJ,Hu Y,Jiang B,Lu L,Zhang X,Liu J,Xu J

    更新日期：2018-03-01 00:00:00

  • Systems-based analysis of RIG-I-dependent signalling identifies KHSRP as an inhibitor of RIG-I receptor activation.

    abstract：:Retinoic acid-inducible gene I (RIG-I) receptor recognizes 5'-triphosphorylated RNA and triggers a signalling cascade that results in the induction of type-I interferon (IFN)-dependent responses. Its precise regulation represents a pivotal balance between antiviral defences and autoimmunity. To elucidate the cellular ...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/nmicrobiol.2017.22

    authors： Soonthornvacharin S,Rodriguez-Frandsen A,Zhou Y,Galvez F,Huffmaster NJ,Tripathi S,Balasubramaniam VR,Inoue A,de Castro E,Moulton H,Stein DA,Sánchez-Aparicio MT,De Jesus PD,Nguyen Q,König R,Krogan NJ,García-Sastre A,Yoh

    更新日期：2017-03-01 00:00:00

  • Phosphatidic acid governs natural egress in Toxoplasma gondii via a guanylate cyclase receptor platform.

    abstract：:Toxoplasma gondii establishes a lifelong chronic infection in humans and animals1. Host cell entry and egress are key steps in the lytic cycle of this obligate intracellular parasite, ensuring its survival and dissemination. Egress is temporally orchestrated, underpinned by the exocytosis of secretory organelles calle...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-018-0339-8

    authors： Bisio H,Lunghi M,Brochet M,Soldati-Favre D

    更新日期：2019-03-01 00:00:00

  • Phenotypic heterogeneity driven by nutrient limitation promotes growth in fluctuating environments.

    abstract：:Most microorganisms live in environments where nutrients are limited and fluctuate over time. Cells respond to nutrient fluctuations by sensing and adapting their physiological state. Recent studies suggest phenotypic heterogeneity(1) in isogenic populations as an alternative strategy in fluctuating environments, wher...

    journal_title：Nature microbiology

    pub_type： 信件

    doi：10.1038/nmicrobiol.2016.55

    authors： Schreiber F,Littmann S,Lavik G,Escrig S,Meibom A,Kuypers MM,Ackermann M

    更新日期：2016-05-09 00:00:00

  • Carcinogenesis and therapeutics: the microbiota perspective.

    abstract：:Cancer arises from the acquisition of multiple genetic and epigenetic changes in host cells over the span of many years, promoting oncogenic traits and carcinogenesis. Most cancers develop following random somatic alterations of key oncogenic genes, which are favoured by a number of risk factors, including lifestyle, ...

    journal_title：Nature microbiology

    pub_type： 杂志文章,评审

    doi：10.1038/nmicrobiol.2017.8

    authors： Tsilimigras MC,Fodor A,Jobin C

    更新日期：2017-02-22 00:00:00

  • A nonlinear time-series analysis approach to identify thresholds in associations between population antibiotic use and rates of resistance.

    abstract：:Balancing access to antibiotics with the control of antibiotic resistance is a global public health priority. At present, antibiotic stewardship is informed by a 'use it and lose it' principle, in which antibiotic use by the population is linearly related to resistance rates. However, theoretical and mathematical mode...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-019-0410-0

    authors： López-Lozano JM,Lawes T,Nebot C,Beyaert A,Bertrand X,Hocquet D,Aldeyab M,Scott M,Conlon-Bingham G,Farren D,Kardos G,Fésűs A,Rodríguez-Baño J,Retamar P,Gonzalo-Jiménez N,Gould IM,THRESHOLDS study group.

    更新日期：2019-07-01 00:00:00

  • The impact of antimicrobials on gonococcal evolution.

    abstract：:The sexually transmitted pathogen Neisseria gonorrhoeae is regarded as being on the way to becoming an untreatable superbug. Despite its clinical importance, little is known about its emergence and evolution, and how this corresponds with the introduction of antimicrobials. We present a genome-based phylogeographical ...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-019-0501-y

    authors： Sánchez-Busó L,Golparian D,Corander J,Grad YH,Ohnishi M,Flemming R,Parkhill J,Bentley SD,Unemo M,Harris SR

    更新日期：2019-11-01 00:00:00

  • Influenza virus mRNA trafficking through host nuclear speckles.

    abstract：:Influenza A virus is a human pathogen with a genome composed of eight viral RNA segments that replicate in the nucleus. Two viral mRNAs are alternatively spliced. The unspliced M1 mRNA is translated into the matrix M1 protein, while the ion channel M2 protein is generated after alternative splicing. These proteins are...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/nmicrobiol.2016.69

    authors： Mor A,White A,Zhang K,Thompson M,Esparza M,Muñoz-Moreno R,Koide K,Lynch KW,García-Sastre A,Fontoura BM

    更新日期：2016-05-27 00:00:00

  • The genome of Onchocerca volvulus, agent of river blindness.

    abstract：:Human onchocerciasis is a serious neglected tropical disease caused by the filarial nematode Onchocerca volvulus that can lead to blindness and chronic disability. Control of the disease relies largely on mass administration of a single drug, and the development of new drugs and vaccines depends on a better knowledge ...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/nmicrobiol.2016.216

    authors： Cotton JA,Bennuru S,Grote A,Harsha B,Tracey A,Beech R,Doyle SR,Dunn M,Hotopp JC,Holroyd N,Kikuchi T,Lambert O,Mhashilkar A,Mutowo P,Nursimulu N,Ribeiro JM,Rogers MB,Stanley E,Swapna LS,Tsai IJ,Unnasch TR,Voronin

    更新日期：2016-11-21 00:00:00

  • Pneumococcal quorum sensing drives an asymmetric owner-intruder competitive strategy during carriage via the competence regulon.

    abstract：:Competition among microorganisms is a key determinant of successful host colonization and persistence. For Streptococcus pneumoniae, lower than predicted rates of co-colonizing strains suggest a competitive advantage for resident bacteria over newcomers. In light of evolutionary theory, we hypothesized that S. pneumon...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-018-0314-4

    authors： Shen P,Lees JA,Bee GCW,Brown SP,Weiser JN

    更新日期：2019-01-01 00:00:00

  • Epstein-Barr virus ncRNA from a nasopharyngeal carcinoma induces an inflammatory response that promotes virus production.

    abstract：:The Epstein-Barr virus M81 strain, isolated from a nasopharyngeal carcinoma, induces potent spontaneous virus production in infected B cells. We found that the M81 non-coding Epstein-Barr-encoded RNA EBER2, which carries polymorphisms that are mainly restricted to viruses found in endemic nasopharyngeal carcinomas, ma...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-019-0546-y

    authors： Li Z,Tsai MH,Shumilov A,Baccianti F,Tsao SW,Poirey R,Delecluse HJ

    更新日期：2019-12-01 00:00:00

  • Insect symbiotic bacteria harbour viral pathogens for transovarial transmission.

    abstract：:Many insects, including mosquitoes, planthoppers, aphids and leafhoppers, are the hosts of bacterial symbionts and the vectors for transmitting viral pathogens1-3. In general, symbiotic bacteria can indirectly affect viral transmission by enhancing immunity and resistance to viruses in insects3-5. Whether symbiotic ba...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/nmicrobiol.2017.25

    authors： Jia D,Mao Q,Chen Y,Liu Y,Chen Q,Wu W,Zhang X,Chen H,Li Y,Wei T

    更新日期：2017-03-06 00:00:00

  • CRAGE enables rapid activation of biosynthetic gene clusters in undomesticated bacteria.

    abstract：:It is generally believed that exchange of secondary metabolite biosynthetic gene clusters (BGCs) among closely related bacteria is an important driver of BGC evolution and diversification. Applying this idea may help researchers efficiently connect many BGCs to their products and characterize the products' roles in va...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-019-0573-8

    authors： Wang G,Zhao Z,Ke J,Engel Y,Shi YM,Robinson D,Bingol K,Zhang Z,Bowen B,Louie K,Wang B,Evans R,Miyamoto Y,Cheng K,Kosina S,De Raad M,Silva L,Luhrs A,Lubbe A,Hoyt DW,Francavilla C,Otani H,Deutsch S,Washton NM,

    更新日期：2019-12-01 00:00:00

  • Clostridium difficile toxins induce VEGF-A and vascular permeability to promote disease pathogenesis.

    abstract：:Clostridium difficile infection (CDI) is mediated by two major exotoxins, toxin A (TcdA) and toxin B (TcdB), that damage the colonic epithelial barrier and induce inflammatory responses. The function of the colonic vascular barrier during CDI has been relatively understudied. Here we report increased colonic vascular ...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-018-0300-x

    authors： Huang J,Kelly CP,Bakirtzi K,Villafuerte Gálvez JA,Lyras D,Mileto SJ,Larcombe S,Xu H,Yang X,Shields KS,Zhu W,Zhang Y,Goldsmith JD,Patel IJ,Hansen J,Huang M,Yla-Herttuala S,Moss AC,Paredes-Sabja D,Pothoulakis C,Shah

    更新日期：2019-02-01 00:00:00

  • Co-occurring genomic capacity for anaerobic methane and dissimilatory sulfur metabolisms discovered in the Korarchaeota.

    abstract：:Phylogenetic and geological evidence supports the hypothesis that life on Earth originated in thermal environments and conserved energy through methanogenesis or sulfur reduction. Here we describe two populations of the deeply rooted archaeal phylum Korarchaeota, which were retrieved from the metagenome of a circumneu...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-019-0362-4

    authors： McKay LJ,Dlakić M,Fields MW,Delmont TO,Eren AM,Jay ZJ,Klingelsmith KB,Rusch DB,Inskeep WP

    更新日期：2019-04-01 00:00:00

  • TRIM23 mediates virus-induced autophagy via activation of TBK1.

    abstract：:Autophagy and interferon (IFN)-mediated innate immunity are critical antiviral defence mechanisms, and recent evidence indicated that tripartite motif (TRIM) proteins are important regulators of both processes. Although the role of TRIM proteins in modulating antiviral cytokine responses has been well established, muc...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-017-0017-2

    authors： Sparrer KMJ,Gableske S,Zurenski MA,Parker ZM,Full F,Baumgart GJ,Kato J,Pacheco-Rodriguez G,Liang C,Pornillos O,Moss J,Vaughan M,Gack MU

    更新日期：2017-11-01 00:00:00

  • Exploitation of the Leishmania exosomal pathway by Leishmania RNA virus 1.

    abstract：:Leishmania are ancient eukaryotes that have retained the exosome pathway through evolution. Leishmania RNA virus 1 (LRV1)-infected Leishmania species are associated with a particularly aggressive mucocutaneous disease triggered in response to the double-stranded RNA (dsRNA) virus. However, it is unclear how LRV1 is ex...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-018-0352-y

    authors： Atayde VD,da Silva Lira Filho A,Chaparro V,Zimmermann A,Martel C,Jaramillo M,Olivier M

    更新日期：2019-04-01 00:00:00

  • VacA generates a protective intracellular reservoir for Helicobacter pylori that is eliminated by activation of the lysosomal calcium channel TRPML1.

    abstract：:Helicobacter pylori infection is a proven carcinogen for gastric cancer. Its virulence factor vacuolating cytotoxin A (VacA) promotes more severe disease and gastric colonization. VacA, by an unknown mechanism, usurps lysosomal and autophagy pathways to generate a protected reservoir for H. pylori that confers bacteri...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-019-0441-6

    authors： Capurro MI,Greenfield LK,Prashar A,Xia S,Abdullah M,Wong H,Zhong XZ,Bertaux-Skeirik N,Chakrabarti J,Siddiqui I,O'Brien C,Dong X,Robinson L,Peek RM Jr,Philpott DJ,Zavros Y,Helmrath M,Jones NL

    更新日期：2019-08-01 00:00:00

  • Publisher Correction: Enterotypes in the landscape of gut microbial community composition.

    abstract：:In the version of this Perspective originally published, the first and last name of co-author Manimozhiyan Arumugam were switched. This has now been corrected in all versions of the Perspective. ...

    journal_title：Nature microbiology

    pub_type： 已发布勘误

    doi：10.1038/s41564-018-0114-x

    authors： Costea PI,Hildebrand F,Arumugam M,Bäckhed F,Blaser MJ,Bushman FD,de Vos WM,Ehrlich SD,Fraser CM,Hattori M,Huttenhower C,Jeffery IB,Knights D,Lewis JD,Ley RE,Ochman H,O'Toole PW,Quince C,Relman DA,Shanahan F,Sunaga

    更新日期：2018-03-01 00:00:00

  • Roles of bacteriophages, plasmids and CRISPR immunity in microbial community dynamics revealed using time-series integrated meta-omics.

    abstract：:Viruses and plasmids (invasive mobile genetic elements (iMGEs)) have important roles in shaping microbial communities, but their dynamic interactions with CRISPR-based immunity remain unresolved. We analysed generation-resolved iMGE-host dynamics spanning one and a half years in a microbial consortium from a biologica...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/s41564-020-00794-8

    authors： Martínez Arbas S,Narayanasamy S,Herold M,Lebrun LA,Hoopmann MR,Li S,Lam TJ,Kunath BJ,Hicks ND,Liu CM,Price LB,Laczny CC,Gillece JD,Schupp JM,Keim PS,Moritz RL,Faust K,Tang H,Ye Y,Skupin A,May P,Muller EEL,Wilm

    更新日期：2021-01-01 00:00:00

  • The anti-inflammatory drug mesalamine targets bacterial polyphosphate accumulation.

    abstract：:Mesalamine serves as the gold standard in treating ulcerative colitis. However, its precise mechanism(s) of action remains unclear. Here, we show that mesalamine treatment rapidly decreases polyphosphate levels in diverse bacteria, including members of the human gut microbiome. This decrease sensitizes bacteria toward...

    journal_title：Nature microbiology

    pub_type： 杂志文章

    doi：10.1038/nmicrobiol.2016.267

    authors： Dahl JU,Gray MJ,Bazopoulou D,Beaufay F,Lempart J,Koenigsknecht MJ,Wang Y,Baker JR,Hasler WL,Young VB,Sun D,Jakob U

    更新日期：2017-01-23 00:00:00