Abstract:
:The dysregulation of intestinal microbial communities is associated with inflammatory bowel diseases (IBD). Studies aimed at understanding the contribution of the microbiota to inflammatory diseases have primarily focused on bacteria, yet the intestine harbours a viral component dominated by prokaryotic viruses known as bacteriophages (phages). Phage numbers are elevated at the intestinal mucosal surface and phages increase in abundance during IBD, suggesting that phages play an unidentified role in IBD. We used a sequence-independent approach for the selection of viral contigs and then applied quantitative metagenomics to study intestinal phages in a mouse model of colitis. We discovered that during colitis the intestinal phage population is altered and transitions from an ordered state to a stochastic dysbiosis. We identified phages specific to pathobiotic hosts associated with intestinal disease, whose abundances are altered during colitis. Additionally, phage populations in healthy and diseased mice overlapped with phages from healthy humans and humans with IBD. Our findings indicate that intestinal phage communities are altered during inflammatory disease, establishing a platform for investigating phage involvement in IBD.
journal_name
Nat Microbioljournal_title
Nature microbiologyauthors
Duerkop BA,Kleiner M,Paez-Espino D,Zhu W,Bushnell B,Hassell B,Winter SE,Kyrpides NC,Hooper LVdoi
10.1038/s41564-018-0210-ysubject
Has Abstractpub_date
2018-09-01 00:00:00pages
1023-1031issue
9issn
2058-5276pii
10.1038/s41564-018-0210-yjournal_volume
3pub_type
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