Abstract:
:Abnormal cardiac fibrosis indicates cardiac dysfunction and poor prognosis in myocardial infarction (MI) patients. Many studies have demonstrated that the ubiquitin proteasome system (UPS) plays a significant role in the pathogenesis of fibrosis. Ubiquitin C-terminal hydrolase L1 (UCHL1), a member of the UPS, is related to fibrosis in several heart diseases. However, whether UCHL1 regulates cardiac fibrosis following MI has yet to be determined. In the present study, we found that UCHL1 was dramatically increased in infarct hearts and TGF-β1-stimulated cardiac fibroblasts (CFs). Inhibition of UCHL1 with LDN57444 (LDN) reversed the myocardial fibrosis in post-MI heart and improved cardiac function. Treatment of LDN or UCHL1 siRNA abolished the TGF-β1-induced fibrotic response of CFs. We further identified GRP78 as an interactor of UCHL1 through screening using immunoprecipitation-mass spectrometer. We determined that UCHL1 interacted with glucose-regulated protein of 78 kDa (GRP78) and prompted GRP78 degradation via ubiquitination. Furthermore, we found that GRP78 was upregulated after UCHL1 knockdown and that the GRP78 inhibitor HA15 diminished the antifibrotic function exerted by UCHL1 knockdown in CFs stimulated with TGF-β1. This suggests that UCHL1 regulates cardiac fibrosis post MI through interactions with GRP78. This work identifies that the UCHL1-GRP78 axis is involved in cardiac fibrosis after MI.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Lei Q,Yi T,Li H,Yan Z,Lv Z,Li G,Wang Ydoi
10.1038/s41598-020-67746-4subject
Has Abstractpub_date
2020-06-30 00:00:00pages
10604issue
1issn
2045-2322pii
10.1038/s41598-020-67746-4journal_volume
10pub_type
杂志文章abstract::Anatomically incomplete spinal cord injuries can be followed by functional recovery mediated, in part, by the formation of intraspinal detour circuits. Here, we show that adult mice recover tactile and proprioceptive function following a unilateral dorsal column lesion. We therefore investigated the basis of this reco...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67866-x
更新日期:2020-07-02 00:00:00
abstract::Hyperactivity of signal transducer and activity of transcription 3 (STAT3) plays a crucial role in melanoma invasion and metastasis. Gene therapy applying siRNA targeting STAT3 is a potential therapeutic strategy for melanoma. In this article, we first fabricated safe and novel dissolving microneedles (MNs) for topica...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19463-2
更新日期:2018-01-18 00:00:00
abstract::Prolonged healing and scar formation are two major challenges in the treatment of soft tissue trauma. Adipose mesenchymal stem cells (ASCs) play an important role in tissue regeneration, and recent studies have suggested that exosomes secreted by stem cells may contribute to paracrine signaling. In this study, we inve...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32993
更新日期:2016-09-12 00:00:00
abstract::Hypertension and diabetes mellitus (DM) have become major public health issues, and previous studies have shown that there is an association between hypertension and DM. However, there is a lack of detailed information about this association. This study aimed to explore how different blood pressure (BP) levels were as...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26323-6
更新日期:2018-05-21 00:00:00
abstract::Growing evidence indicates that nomogram combined with the biomarkers of systemic inflammation response could provide more accurate prediction than conventional staging systems in tumors. This study aimed to establish an effective prognostic nomogram for resectable thoracic esophageal squamous cell carcinoma (ESCC) ba...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18811
更新日期:2015-12-22 00:00:00
abstract::The nuclear disaster at the Fukushima Daiichi Nuclear Power Plant (FDNPP) in March 2011 caused partial meltdowns of three reactors. During the meltdowns, a type of condensed particle, a caesium-rich micro-particle (CsMP), formed inside the reactors via unknown processes. Here we report the chemical and physical proces...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42731
更新日期:2017-02-15 00:00:00
abstract::Accumulating evidence indicates the absence of paternally derived miRNAs, piwiRNAs, and proteins may be one important factor contributing to developmental failure in somatic cell cloned embryos. In the present study, we found microRNA-449b (miR-449b) was highly expressed in sperm. Target gene predictions and experimen...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13899-8
更新日期:2017-10-17 00:00:00
abstract::A bistable toggle switch is a paradigmatic model in the field of biology. The dynamics of the system induced by Gaussian noise has been intensively investigated, but Gaussian noise cannot incorporate large bursts typically occurring in real experiments. This paper aims to examine effects of variations from one protein...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep31505
更新日期:2016-08-19 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-60642-x
更新日期:2020-02-21 00:00:00
abstract::During our research, we explored a novel way to represent subwavelength imaging and derived a transmission equation to explicate the FP (Fabry-Pérot) resonance phenomena. Subsequently, using analysis and observation, we performed deep-subwavelength imaging. Both numerically and experimentally, imaging with super-resol...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67409-4
更新日期:2020-07-01 00:00:00
abstract::Rivers are an important aquatic conduit that connects terrestrial sources of dissolved inorganic carbon (DIC) and other elements with oceanic reservoirs. The Mekong River, one of the world's largest rivers, is firstly examined to explore inter-annual fluxes of dissolved and particulate constituents during 1923-2011 an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16005
更新日期:2015-11-02 00:00:00
abstract::Pre-shared non-local entanglement dramatically simplifies and improves the performance of quantum error correction via entanglement-assisted quantum error-correcting codes (EAQECCs). However, even considering the noise in quantum communication only, the non-local sharing of a perfectly entangled pair is technically im...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07203
更新日期:2014-11-26 00:00:00
abstract::How diverse adult stem and progenitor populations regenerate tissue following damage to the brain is poorly understood. In highly regenerative vertebrates, such as zebrafish, radial-glia (RG) and neuro-epithelial-like (NE) stem/progenitor cells contribute to neuronal repair after injury. However, not all RG act as neu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40734-z
更新日期:2019-03-14 00:00:00
abstract::3D printing has produced many beneficial applications for surgery. The technique´s applicability in replicating nasal cavity anatomy for clinical use has not been studied. Our aim was to determine whether 3D printing could realistically replicate the nasal cavities and the airflow passing through them from a clinical ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-57537-2
更新日期:2020-01-16 00:00:00
abstract::Ca2+ release-activated Ca2+ (CRAC) channels elevate cytoplasmic Ca2+ concentration, which is essential for T cell activation, differentiation and effector functions. T cell receptor stimulation induces depletion of the endoplasmic reticulum (ER) Ca2+ stores, which is sensed by stromal interaction molecule 1 (STIM1). S...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71489-7
更新日期:2020-09-02 00:00:00
abstract::Aquaculture has become imperative to cover the demands for dietary animal protein. Simultaneously, it has to overcome prejudices from excessive use of antibiotics and environmental impacts. Natural supplements are traditionally applied orally. In this study, we demonstrated another pathway: the gills. Humic substances...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80449-0
更新日期:2021-01-08 00:00:00
abstract::The serotonin transporter (SERT) is an integral membrane protein that exploits preexisting sodium-, chloride-, and potassium ion gradients to catalyze the thermodynamically unfavorable movement of synaptic serotonin into the presynaptic neuron. SERT has garnered significant clinical attention partly because it is the ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23789
更新日期:2016-04-01 00:00:00
abstract::Among the multiple metabolic signals involved in the establishment of the hepatic zonation, oxygen could play a key role. Indeed, depending on hepatocyte position in the hepatic lobule, gene expression and metabolism are differently affected by the oxygen gradient present across the lobule. The aim of this study is to...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49412-6
更新日期:2019-09-19 00:00:00
abstract::Global lake systems have undergone rapid degradation over the past century. Scientists and managers are struggling to manage the highly degraded lake systems to cope with escalating anthropogenic pressures. Improved knowledge of how lakes and social systems co-evolved up to the present is vital for understanding, mode...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35482-5
更新日期:2018-11-21 00:00:00
abstract::An efficient [4 + 1] annulation between α-bromooximes and sulfur ylides via in situ generation of nitrosoalkenes under mild basic reaction conditions has been developed, providing an expeditious and scalable approach to synthesize biologically interesting isoxazoline derivatives with good to excellent yields. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-81370-w
更新日期:2021-01-22 00:00:00
abstract::Water, methane, and ammonia are commonly considered to be the key components of the interiors of Uranus and Neptune. Modelling the planets' internal structure, evolution, and dynamo heavily relies on the properties of the complex mixtures with uncertain exact composition in their deep interiors. Therefore, characteris...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46561-6
更新日期:2019-07-12 00:00:00
abstract::Animals execute sensorimotor sequences to optimize performance of complex actions series. However, the sensory aspects of these sequences and their dynamic control are often poorly understood. We trained bats to fly to targets at different distances, and analysed their sensory behavior before and during flight to test...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00543-8
更新日期:2017-03-28 00:00:00
abstract::Tinnitus (phantom auditory perception associated with hearing loss) can seriously affect wellbeing. Its neural substrate is unknown however it has been linked with abnormal activity in auditory pathways. Though no cure currently exists, repetitive transcranial magnetic stimulation (rTMS) has been shown to reduce tinni...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38234
更新日期:2016-12-01 00:00:00
abstract::Lonicera japonica Thunb, rich in chlorogenic acid (CHA), is used for viral upper respiratory tract infection treatment caused by influenza virus, parainfluenza virus, and respiratory syncytial virus, ect in China. It was reported that CHA reduced serum hepatitis B virus level and death rate of influenza virus-infected...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45723
更新日期:2017-04-10 00:00:00
abstract::Nitrogen-doped graphene oxides (GO:Nx) were synthesized by a partial reduction of graphene oxide (GO) using urea [CO(NH2)2]. Their electronic/bonding structures were investigated using X-ray absorption near-edge structure (XANES), valence-band photoemission spectroscopy (VB-PES), X-ray emission spectroscopy (XES) and ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42235
更新日期:2017-02-10 00:00:00
abstract::Graphene quantum dots (GQDs) have received much attention due to their novel phenomena of charge transport and light absorption/emission. The optical transitions are known to be available up to ~6 eV in GQDs, especially useful for ultraviolet (UV) photodetectors (PDs). Thus, the demonstration of photodetection gain wi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05603
更新日期:2014-07-07 00:00:00
abstract::Enzyme replacement therapies have revolutionized patient treatment for multiple rare lysosomal storage diseases but show limited effectiveness for addressing pathologies in "hard-to-treat" organs and tissues including brain and bone. Here we investigate the plant lectin RTB as a novel carrier for human lysosomal enzym...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14144
更新日期:2015-09-18 00:00:00
abstract::The microbiota colonized on human body is renowned as "a forgotten organ" due to its big impacts on human health and disease. Recently, microbiome studies have identified a large number of microbes differentially regulated in a variety of conditions, such as disease and diet. However, methods for discovering biologica...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40200
更新日期:2017-01-10 00:00:00
abstract::Collateral perfusion is important for sustaining tissue viability in acute ischemic stroke. Conventional techniques for its visualization are invasive, require contrast agents and demonstrate collateral vessels, rather than measuring perfusion directly. In this study we utilize a non-invasive, non-contrast magnetic re...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-44417-7
更新日期:2019-06-03 00:00:00
abstract::Oxytocin (OXT) is an important neuromodulator of social behaviors via activation of both oxytocin receptors (OXTR) and vasopressin (AVP) 1a receptors (AVPR1a). Marmosets are neotropical primates with a modified OXT ligand (Pro8-OXT), and this ligand shows significant coevolution with traits including social monogamy a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52024-9
更新日期:2019-10-29 00:00:00