Abstract：
:There is an ongoing worldwide coronavirus disease 2019 (Covid-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). At present, confirmatory diagnosis is by reverse transcription polymerase chain reaction (RT-PCR), typically taking several hours and requiring a molecular laboratory to perform. There is an urgent need for rapid, simplified, and cost-effective detection methods. We have developed and analytically validated a protocol for direct rapid extraction-free PCR (DIRECT-PCR) detection of SARS-CoV-2 without the need for nucleic acid purification. As few as six RNA copies per reaction of viral nucleocapsid (N) gene from respiratory samples such as sputum and nasal exudate can be detected directly using our one-step inhibitor-resistant assay. The performance of this assay was validated on a commercially available portable PCR thermocycler. Viral lysis, reverse transcription, amplification, and detection are achieved in a single-tube homogeneous reaction within 36 min. This minimizes hands-on time, reduces turnaround-time for sample-to-result, and obviates the need for RNA purification reagents. It could enable wider use of Covid-19 testing for diagnosis, screening, and research in countries and regions where laboratory capabilities are limiting.
journal_name
Genes (Basel)journal_title
Genesauthors
Wee SK,Sivalingam SP,Yap EPHdoi
10.3390/genes11060664subject
Has Abstractpub_date
2020-06-18 00:00:00issue
6issn
2073-4425pii
genes11060664journal_volume
11pub_type
杂志文章相关文献
Genes文献大全abstract：:Juvenile open angle glaucoma (JOAG), which is an uncommon form of primary open angle glaucoma, is a clinically and genetically heterogeneous disorder. We report on a family with a recessively inherited form of JOAG. The proband has a superior and an inferior never fiber layer thinning in both the eyes and the nasal vi...
journal_title：Genes
pub_type：
doi：10.3390/genes9110527
更新日期：2018-10-30 00:00:00
abstract：:The wall-associated kinase (WAK) and wall-associated kinase like (WAKL) is a subfamily of receptor-like kinases associated with the cell wall, which have been suggested as sensors of the extracellular environment and triggers of intracellular signals. However, these proteins have not yet been comprehensively analyzed ...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11101186
更新日期：2020-10-12 00:00:00
abstract：:The telomerase RNA in yeasts is large, usually >1000 nt, and contains functional elements that have been extensively studied experimentally in several disparate species. Nevertheless, they are very difficult to detect by homology-based methods and so far have escaped annotation in the majority of the genomes of Saccha...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes9080372
更新日期：2018-07-26 00:00:00
abstract：:The spectrum of modern horse populations encompasses populations with a long history of development in isolation and relatively recently formed types. To increase our understanding of the evolutionary history and provide information on how to optimally conserve or improve these populations with varying development and...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes10060480
更新日期：2019-06-25 00:00:00
abstract：:DNA methylation, catalyzed by DNA methyltransferases (DNMTs), is an epigenetic mark that needs to be faithfully replicated during mitosis in order to maintain cell phenotype during successive cell divisions. This epigenetic mark is located on the 5'-carbon of the cytosine mainly within cytosine⁻phosphate⁻guanine (CpG)...
journal_title：Genes
pub_type： 杂志文章,评审
doi：10.3390/genes10010065
更新日期：2019-01-18 00:00:00
abstract：:Although bacterial genomes have been traditionally viewed as being very compact, with relatively low amounts of repetitive and non-coding DNA, this view has dramatically changed in recent years. The increase of available complete bacterial genomes has revealed that many species present abundant repetitive DNA (i.e., i...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes3040634
更新日期：2012-10-12 00:00:00
abstract：:Changes of telomere length with age were assessed in diploid and triploid rainbow trout (Oncorhynchus mykiss) females in the cross-sectional study using Q-FISH technique. Triploid trout as sterile do not invest an energy in gametogenesis and continue to grow, whereas fertile diploid individuals suffer from declines in...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11070786
更新日期：2020-07-13 00:00:00
abstract：:Vitellogenin receptors (VgRs) play critical roles in egg formation by transporting vitellogenin (Vg) into oocytes in insects. Although the function of VgR in insects is well studied, the transcriptional regulation of this gene is still unclear. Here, we cloned the promoter of the VgR gene from Bombyx mori (BmVgR), and...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11040394
更新日期：2020-04-06 00:00:00
abstract：:Quorum sensing (QS) is a cell-to-cell communication system that uses autoinducers as signaling molecules to enable inter-species and intra-species interactions in response to external stimuli according to the population density. QS allows bacteria such as Acinetobacter baumannii to react rapidly in response to environ...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes10040282
更新日期：2019-04-08 00:00:00
abstract：:Almost all eukaryotes have transposable elements (TEs) against which they have developed defense mechanisms. In the Drosophila germline, the main transposable element (TE) regulation pathway is mediated by specific Piwi-interacting small RNAs (piRNAs). Nonetheless, for unknown reasons, TEs sometimes escape cellular co...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11020215
更新日期：2020-02-19 00:00:00
abstract：:Serotonin receptor 2A (HTR2A) is an important signalling factor implicated in cognitive functions and known to be associated with schizophrenia. The biological significance of HTR2A in schizophrenia remains unclear as molecular analyses including genetic association, mRNA expression and methylation studies have report...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes8010014
更新日期：2017-01-04 00:00:00
abstract：:The yeast Exophiala dermatitidis exhibits high resistance to γ-radiation in comparison to many other fungi. Several aspects of this phenotype have been characterized, including its dependence on homologous recombination for the repair of radiation-induced DNA damage, and the transcriptomic response invoked by acute γ-...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11101128
更新日期：2020-09-25 00:00:00
abstract：:Due to its extraordinary longevity and wide distribution, the ocean quahog Arctica islandica has become an important species model in both aging and environmental change research. Notwithstanding that, most genetic studies on ocean quahogs have been focused on fishery related, phylogeographic and phylogenetic aspects ...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes9060299
更新日期：2018-06-13 00:00:00
abstract：:Modulated electromagnetic fields (wEMFs), as generated by modern communication technologies, have raised concerns about adverse health effects. The International Agency for Research on Cancer (IARC) classifies them as "possibly carcinogenic to humans" (Group 2B), yet, the underlying molecular mechanisms initiating and...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11040347
更新日期：2020-03-25 00:00:00
abstract：:For medical genetic counseling, estimating the chance of a child being born with chromosome abnormality is crucially important. Cytogenetic diagnostics of parents with a balanced karyotype are a special case. Such chromosome rearrangements cannot be detected with comprehensive chromosome screening. In the current pape...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11121511
更新日期：2020-12-17 00:00:00
abstract：:The Sichuan partridge (Arborophila rufipectus, Phasianidae, Galliformes) is distributed in south-west China, and classified as endangered grade. To examine the evolution and genomic features of Sichuan partridge, we de novo assembled the Sichuan partridge reference genome. The final draft assembly consisted of approxi...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes10090677
更新日期：2019-09-05 00:00:00
abstract：:The large phenotypic variation in the olfactory bulb may be related to heterogeneity in the progenitor cells. Accordingly, the progeny of subventricular zone (SVZ) progenitor cells that are destined for the olfactory bulb is of particular interest, specifically as there are many facets of these progenitors and their m...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11030305
更新日期：2020-03-13 00:00:00
abstract：:B chromosomes (Bs) are enigmatic additional elements in the genomes of thousands of species of plants, animals, and fungi. How do these non-essential, harmful, and parasitic chromosomes maintain their presence in their hosts, making demands on all the essential functions of their host genomes? The answer seems to be t...
journal_title：Genes
pub_type： 杂志文章,评审
doi：10.3390/genes9080388
更新日期：2018-07-31 00:00:00
abstract：:As a highly nutritious crop, Tartary buckwheat (Fagopyrum tartaricum) strongly adapts and grows in adverse environments and is widely grown in Asia. However, its flour contains a large proportion of the hull that adheres to the testa layer of the groats and is difficult to be removed in industrial processing. Fortunat...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11040459
更新日期：2020-04-23 00:00:00
abstract：:Wnt is a complex signaling pathway involved in the regulation of crucial biological functions such as development, proliferation, differentiation and migration of cells, mainly stem cells, which are virtually present in all embryonic and adult tissues. Conversely, dysregulation of Wnt signal is implicated in developme...
journal_title：Genes
pub_type： 杂志文章,评审
doi：10.3390/genes9020105
更新日期：2018-02-17 00:00:00
abstract：:Gene expression in the brain is dramatically regulated by a variety of stimuli. While the role of neural activity has been extensively studied, less is known about the effects of metabolism and nutrition on transcriptional control mechanisms in the brain. Extracellular signals are integrated at the chromatin level thr...
journal_title：Genes
pub_type： 杂志文章,评审
doi：10.3390/genes11070742
更新日期：2020-07-03 00:00:00
abstract：BACKGROUND:HER2 (human epidermal growth factor 2)-positive breast cancer is an aggressive type of breast cancer characterized by the overexpression of the receptor-type protein tyrosine kinase HER2 or amplification of the HER2 gene. It is commonly treated by the drug trastuzumab (Herceptin), but resistance to its actio...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes10080568
更新日期：2019-07-26 00:00:00
abstract：:Fragile X syndrome (FXS) is the most common inherited cause of intellectual disability and autism spectrum disorder, and among those with fragile X syndrome, approximately 1/3rd meet a threshold for an autism spectrum disorder (ASD) diagnosis. Previous functional imaging studies of fragile X syndrome have typically fo...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes10121052
更新日期：2019-12-17 00:00:00
abstract：:The scale of genetic methods are presently being expanded: forensic genetic assays previously were limited to tens of loci, but now technologies allow for a transition to forensic genomic approaches that assess thousands to millions of loci. However, there are subtle distinctions between genetic assays and their genom...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes12020185
更新日期：2021-01-27 00:00:00
abstract：:In addition to its wide geographical distribution, osteoglossiform fishes represent one of the most ancient freshwater teleost lineages; making it an important group for systematic and evolutionary studies. These fishes had a Gondwanan origin and their past distribution may have contributed to the diversity present in...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes9060306
更新日期：2018-06-19 00:00:00
abstract：:Cell size affects almost all biosynthetic processes by controlling the size of organelles and disrupting the nutrient uptake process. Yeast cells must reach a critical size to be able to enter a new cell cycle stage. Abnormal changes in cell size are often observed under pathological conditions such as cancer disease....
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11040430
更新日期：2020-04-16 00:00:00
abstract：:While methods for detecting SNVs and indels in circulating tumor DNA (ctDNA) with hybridization capture-based next-generation sequencing (NGS) have been available, copy number variations (CNVs) detection is more challenging. Here, we present a method enabling CNV detection from a 150-gene panel using a very low amount...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes10110926
更新日期：2019-11-14 00:00:00
abstract：:DNA double-strand breaks (DSBs) jeopardize genome integrity and can-when repaired unfaithfully-give rise to structural rearrangements associated with cancer. Exogenous agents such as ionizing radiation or chemotherapy can invoke DSBs, but a vast amount of breakage arises during vital endogenous DNA transactions, such ...
journal_title：Genes
pub_type： 杂志文章,评审
doi：10.3390/genes9120632
更新日期：2018-12-14 00:00:00
abstract：:Copy Number Variations (CNVs) and Single Nucleotide Polymorphisms (SNPs) have been the major focus of most large-scale comparative genomics studies to date. Here, we discuss a third, largely ignored, type of genetic variation, namely changes in tandem repeat number. Historically, tandem repeats have been designated as...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes3030461
更新日期：2012-07-26 00:00:00
abstract：:The transition from a dormant to a germinating seed represents a crucial developmental switch in the life cycle of a plant. Subsequent transition from a germinating seed to an autotrophic organism also requires a robust and multi-layered control. Seed germination and seedling growth are multistep processes, involving ...
journal_title：Genes
pub_type： 杂志文章,评审
doi：10.3390/genes12010052
更新日期：2020-12-31 00:00:00
