mRNA Expression and DNA Methylation Analysis of Serotonin Receptor 2A (HTR2A) in the Human Schizophrenic Brain.

Abstract:

:Serotonin receptor 2A (HTR2A) is an important signalling factor implicated in cognitive functions and known to be associated with schizophrenia. The biological significance of HTR2A in schizophrenia remains unclear as molecular analyses including genetic association, mRNA expression and methylation studies have reported inconsistent results. In this study, we examine HTR2A expression and methylation and the interaction with HTR2A polymorphisms to identify their biological significance in schizophrenia. Subjects included 25 schizophrenia and 25 control post-mortem brain samples. Genotype and mRNA data was generated by transcriptome sequencing. DNA methylation profiles were generated for CpG sites within promoter-exon I region. Expression, genotype and methylation data were examined for association with schizophrenia. HTR2A mRNA levels were reduced by 14% (p = 0.006) in schizophrenia compared to controls. Three CpG sites were hypermethylated in schizophrenia (cg5 p = 0.028, cg7 p = 0.021, cg10 p = 0.017) and HTR2A polymorphisms rs6314 (p = 0.008) and rs6313 (p = 0.026) showed genetic association with schizophrenia. Differential DNA methylation was associated with rs6314 and rs6313. There was a strong correlation between HTR2A DNA methylation and mRNA expression. The results were nominally significant but did not survive the rigorous Benjamini-Hochberg correction for multiple testing. Differential HTR2A expression in schizophrenia in our study may be the result of the combined effect of multiple differentially methylated CpG sites. Epigenetic HTR2A regulation may alter brain function, which contributes to the development of schizophrenia.

journal_name

Genes (Basel)

journal_title

Genes

authors

Cheah SY,Lawford BR,Young RM,Morris CP,Voisey J

doi

10.3390/genes8010014

subject

Has Abstract

pub_date

2017-01-04 00:00:00

issue

1

issn

2073-4425

pii

genes8010014

journal_volume

8

pub_type

杂志文章

相关文献

Genes文献大全
  • Unravelling the Roles of Susceptibility Loci for Autoimmune Diseases in the Post-GWAS Era.

    abstract::Although genome-wide association studies (GWAS) have identified several hundred loci associated with autoimmune diseases, their mechanistic insights are still poorly understood. The human genome is more complex than single nucleotide polymorphisms (SNPs) that are interrogated by GWAS arrays. Apart from SNPs, it also c...

    journal_title:Genes

    pub_type: 杂志文章,评审

    doi:10.3390/genes9080377

    authors: Ye J,Gillespie KM,Rodriguez S

    更新日期:2018-07-27 00:00:00

  • TALEN-Mediated Gene Targeting for Cystic Fibrosis-Gene Therapy.

    abstract::Cystic fibrosis (CF) is an inherited monogenic disorder, amenable to gene-based therapies. Because CF lung disease is currently the major cause of mortality and morbidity, and the lung airway is readily accessible to gene delivery, the major CF gene therapy effort at present is directed to the lung. Although airway ep...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes10010039

    authors: Xia E,Zhang Y,Cao H,Li J,Duan R,Hu J

    更新日期:2019-01-11 00:00:00

  • The Genomic Makeup of Nine Horse Populations Sampled in the Netherlands.

    abstract::The spectrum of modern horse populations encompasses populations with a long history of development in isolation and relatively recently formed types. To increase our understanding of the evolutionary history and provide information on how to optimally conserve or improve these populations with varying development and...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes10060480

    authors: Schurink A,Shrestha M,Eriksson S,Bosse M,Bovenhuis H,Back W,Johansson AM,Ducro BJ

    更新日期:2019-06-25 00:00:00

  • NF-YA Overexpression in Lung Cancer: LUSC.

    abstract::The CCAAT box is recognized by the trimeric transcription factor NF-Y, whose NF-YA subunit is present in two major splicing isoforms, NF-YAl ("long") and NF-YAs ("short"). Little is known about the expression levels of NF-Y subunits in tumors, and nothing in lung cancer. By interrogating RNA-seq TCGA and GEO datasets,...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes10110937

    authors: Bezzecchi E,Ronzio M,Dolfini D,Mantovani R

    更新日期:2019-11-17 00:00:00

  • Alternative Splicing in the Hippo Pathway-Implications for Disease and Potential Therapeutic Targets.

    abstract::Alternative splicing is a well-studied gene regulatory mechanism that produces biological diversity by allowing the production of multiple protein isoforms from a single gene. An involvement of alternative splicing in the key biological signalling Hippo pathway is emerging and offers new therapeutic avenues. This revi...

    journal_title:Genes

    pub_type: 杂志文章,评审

    doi:10.3390/genes9030161

    authors: Porazinski S,Ladomery M

    更新日期:2018-03-13 00:00:00

  • Differential Enzymatic Activity of Rat ADAR2 Splicing Variants Is Due to Altered Capability to Interact with RNA in the Deaminase Domain.

    abstract::In mammals, adenosine (A) to inosine (I) RNA editing is performed by adenosine deaminases acting on RNA (ADAR), ADAR1 and ADAR2 enzymes, encoded by mRNAs that might undergo splicing process. In rat, two splicing events produce several isoforms of ADAR2, called ADAR2a, ADAR2b, ADAR2e, and ADAR2f, but only ADAR2a and AD...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes9020079

    authors: Filippini A,Bonini D,Giacopuzzi E,La Via L,Gangemi F,Colombi M,Barbon A

    更新日期:2018-02-08 00:00:00

  • Characterization of the IS200/IS605 Insertion Sequence Family in Halanaerobium Hydrogeniformans.

    abstract::Mobile DNA elements play a significant evolutionary role by promoting genome plasticity. Insertion sequences are the smallest prokaryotic transposable elements. They are highly diverse elements, and the ability to accurately identify, annotate, and infer the full genomic impact of insertion sequences is lacking. Halan...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes11050484

    authors: Sadler M,R Mormile M,L Frank R

    更新日期:2020-04-29 00:00:00

  • Evolutionary Insights of the ZW Sex Chromosomesin Snakes: A New Chapter Added by the AmazonianPuffing Snakes of the Genus Spilotes.

    abstract::Amazonian puffing snakes (Spilotes; Colubridae) are snakes widely distributed in the Neotropical region. However, chromosomal data are scarce in this group and, when available, are only limited to karyotype description using conventional staining. In this paper, we focused on the process of karyotype evolution and tre...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes10040288

    authors: Viana PF,Ezaz T,de Bello Cioffi M,Jackson Almeida B,Feldberg E

    更新日期:2019-04-09 00:00:00

  • High-Throughput Sequencing of Small RNA Transcriptomes in Maize Kernel Identifies miRNAs Involved in Embryo and Endosperm Development.

    abstract::Maize kernel development is a complex biological process that involves the temporal and spatial expression of many genes and fine gene regulation at a transcriptional and post-transcriptional level, and microRNAs (miRNAs) play vital roles during this process. To gain insight into miRNA-mediated regulation of maize ker...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes8120385

    authors: Xing L,Zhu M,Zhang M,Li W,Jiang H,Zou J,Wang L,Xu M

    更新日期:2017-12-14 00:00:00

  • Proteomics Reveals Distinct Changes Associated with Increased Gamma Radiation Resistance in the Black Yeast Exophiala dermatitidis.

    abstract::The yeast Exophiala dermatitidis exhibits high resistance to γ-radiation in comparison to many other fungi. Several aspects of this phenotype have been characterized, including its dependence on homologous recombination for the repair of radiation-induced DNA damage, and the transcriptomic response invoked by acute γ-...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes11101128

    authors: Schultzhaus ZS,Schultzhaus JN,Romsdahl J,Chen A,Hervey Iv WJ,Leary DH,Wang Z

    更新日期:2020-09-25 00:00:00

  • Genome-Wide Identification, Characterization and Expression Profiling of ADF Family Genes in Solanum lycopersicum L.

    abstract::The actin depolymerizing factor (ADF) proteins have growth, development, defense-related and growth regulatory functions in plants. The present study used genome-wide analysis to investigate ADF family genes in tomato. Eleven tomato ADF genes were identified and differential expression patterns were found in different...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes7100079

    authors: Khatun K,Robin AH,Park JI,Kim CK,Lim KB,Kim MB,Lee DJ,Nou IS,Chung MY

    更新日期:2016-09-29 00:00:00

  • The evolution of protein structures and structural ensembles under functional constraint.

    abstract::Protein sequence, structure, and function are inherently linked through evolution and population genetics. Our knowledge of protein structure comes from solved structures in the Protein Data Bank (PDB), our knowledge of sequence through sequences found in the NCBI sequence databases (http://www.ncbi.nlm.nih.gov/), and...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes2040748

    authors: Siltberg-Liberles J,Grahnen JA,Liberles DA

    更新日期:2011-10-28 00:00:00

  • Computational Strategies for Scalable Genomics Analysis.

    abstract::The revolution in next-generation DNA sequencing technologies is leading to explosive data growth in genomics, posing a significant challenge to the computing infrastructure and software algorithms for genomics analysis. Various big data technologies have been explored to scale up/out current bioinformatics solutions ...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes10121017

    authors: Shi L,Wang Z

    更新日期:2019-12-06 00:00:00

  • Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats.

    abstract::Satellite DNAs are tandemly repeated sequences preferentially assembled into large arrays within constitutive heterochromatin and their transcription is often activated by stress conditions, particularly by heat stress. Bioinformatic analyses of sequenced genomes however reveal single repeats or short arrays of satell...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes11060663

    authors: Feliciello I,Sermek A,Pezer Ž,Matulić M,Ugarković Đ

    更新日期:2020-06-18 00:00:00

  • Characterization, Expression, and Interaction Analyses of OsMORF Gene Family in Rice.

    abstract::The multiple organellar RNA editing factors (MORF) gene family plays a key role in organelle RNA editing in flowering plants. MORF genes expressions are also affected by abiotic stress. Although seven OsMORF genes have been identified in rice, few reports have been published on their expression patterns in different t...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes10090694

    authors: Zhang Q,Shen L,Ren D,Hu J,Chen G,Zhu L,Gao Z,Zhang G,Guo L,Zeng D,Qian Q

    更新日期:2019-09-10 00:00:00

  • Abnormal base excision repair at trinucleotide repeats associated with diseases: a tissue-selective mechanism.

    abstract::More than fifteen genetic diseases, including Huntington's disease, myotonic dystrophy 1, fragile X syndrome and Friedreich ataxia, are caused by the aberrant expansion of a trinucleotide repeat. The mutation is unstable and further expands in specific cells or tissues with time, which can accelerate disease progressi...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes4030375

    authors: Goula AV,Merienne K

    更新日期:2013-07-25 00:00:00

  • Environmental Change-Dependent Inherited Epigenetic Response.

    abstract::Epigenetic modifications are a mechanism conveying environmental information to subsequent generations via parental germ lines. Research on epigenetic responses to environmental changes in wild mammals has been widely neglected, as well as studies that compare responses to changes in different environmental factors. H...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes10010004

    authors: Weyrich A,Lenz D,Fickel J

    更新日期:2018-12-21 00:00:00

  • Cytogenetics in Arctica islandica (Bivalvia, Arctidae): the Longest Lived Non-Colonial Metazoan.

    abstract::Due to its extraordinary longevity and wide distribution, the ocean quahog Arctica islandica has become an important species model in both aging and environmental change research. Notwithstanding that, most genetic studies on ocean quahogs have been focused on fishery related, phylogeographic and phylogenetic aspects ...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes9060299

    authors: García-Souto D,Pasantes JJ

    更新日期:2018-06-13 00:00:00

  • BACE1 Inhibition Using 2'-OMePS Steric Blocking Antisense Oligonucleotides.

    abstract::Amyloid beta-peptide is produced by the cleavage of amyloid precursor protein by two secretases, a β-secretase, beta-site amyloid precursor protein cleaving enzyme 1 (BACE1) and a γ-secretase. It has been hypothesised that partial inhibition of BACE1 in individuals with a high risk of developing Alzheimer's disease ma...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes10090705

    authors: Chakravarthy M,Veedu RN

    更新日期:2019-09-12 00:00:00

  • Transcriptomic Analysis Reveals Involvement of the Macrophage Migration Inhibitory Factor Gene Network in Duchenne Muscular Dystrophy.

    abstract::Duchenne muscular dystrophy (DMD) is a progressive hereditary muscular disease with X-linked recessive inheritance, that leads patients to premature death. The loss of dystrophin determines membrane instability, causing cell damage and inflammatory response. Macrophage migration inhibitory factor (MIF) is a cytokine t...

    journal_title:Genes

    pub_type: 杂志文章,meta分析

    doi:10.3390/genes10110939

    authors: Lombardo SD,Mazzon E,Mangano K,Basile MS,Cavalli E,Mammana S,Fagone P,Nicoletti F,Petralia MC

    更新日期:2019-11-18 00:00:00

  • From the Outside to the Inside: New Insights on the Main Factors That Guide Seed Dormancy and Germination.

    abstract::The transition from a dormant to a germinating seed represents a crucial developmental switch in the life cycle of a plant. Subsequent transition from a germinating seed to an autotrophic organism also requires a robust and multi-layered control. Seed germination and seedling growth are multistep processes, involving ...

    journal_title:Genes

    pub_type: 杂志文章,评审

    doi:10.3390/genes12010052

    authors: Longo C,Holness S,De Angelis V,Lepri A,Occhigrossi S,Ruta V,Vittorioso P

    更新日期:2020-12-31 00:00:00

  • The Chloroplast Genome Sequence of Scutellaria baicalensis Provides Insight into Intraspecific and Interspecific Chloroplast Genome Diversity in Scutellaria.

    abstract::Scutellaria baicalensis Georgi (Lamiaceae) is the source of the well-known traditional Chinese medicine "HuangQin" (Radix Scutellariae). Natural sources of S. baicalensis are rapidly declining due to high market demand and overexploitation. Moreover, the commercial products of Radix Scutellariae have often been found ...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes8090227

    authors: Jiang D,Zhao Z,Zhang T,Zhong W,Liu C,Yuan Q,Huang L

    更新日期:2017-09-13 00:00:00

  • Predominance of Dystrophinopathy Genotypes in Mexican Male Patients Presenting as Muscular Dystrophy with A Normal Multiplex Polymerase Chain Reaction DMD Gene Result: A Study Including Targeted Next-Generation Sequencing.

    abstract::The complete mutational spectrum of dystrophinopathies and limb-girdle muscular dystrophy (LGMD) remains unknown in Mexican population. Seventy-two unrelated Mexican male patients (73% of pediatric age) with clinical suspicion of muscular dystrophy and no evidence of DMD gene deletion on multiplex polymerase chain rea...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes10110856

    authors: Alcántara-Ortigoza MA,Reyna-Fabián ME,González-Del Angel A,Estandia-Ortega B,Bermúdez-López C,Cruz-Miranda GM,Ruíz-García M

    更新日期:2019-10-29 00:00:00

  • Plant TDP1 (Tyrosyl-DNA Phosphodiesterase 1): A Phylogenetic Perspective and Gene Expression Data Mining.

    abstract::The TDP1 (tyrosyl-DNA phosphodiesterase 1) enzyme removes the non-specific covalent intermediates between topoisomerase I and DNA, thus playing a crucial role in preventing DNA damage. While mammals possess only one TDP1 gene, in plants two genes (TDP1α and TDP1β) are present constituting a small gene subfamily. These...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes11121465

    authors: Mutti G,Raveane A,Pagano A,Bertolini F,Semino O,Balestrazzi A,Macovei A

    更新日期:2020-12-07 00:00:00

  • Allelic imbalances in radiation-associated acute myeloid leukemia.

    abstract::Acute myeloid leukemia (AML) can develop as a secondary malignancy following radiotherapy, but also following low-dose environmental or occupational radiation exposure. Therapy-related AML frequently carries deletions of chromosome 5q and/or 7, but for low-dose exposure associated AML this has not been described. For ...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes2020384

    authors: Klymenko SV,Smida J,Atkinson MJ,Bebeshko VG,Nathrath M,Rosemann M

    更新日期:2011-05-31 00:00:00

  • miR-101b Regulates Lipid Deposition and Metabolism of Primary Hepatocytes in Teleost Yellow Catfish Pelteobagrus fulvidraco.

    abstract::Excessive fat deposition in the hepatocytes, associated with excess dietary fat intake, was related to the occurrence of fatty livers in fish. miR-101b plays the important roles in controlling lipid metabolism, but the underlying mechanism at the post-transcriptional level remains unclear. The purpose of this study is...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes11080861

    authors: Chen GH,Zhao T,Wei XL,Zhang DG,Zhuo MQ,Luo Z

    更新日期:2020-07-29 00:00:00

  • Transcriptome Analysis of Landrace Pig Subcutaneous Preadipocytes during Adipogenic Differentiation.

    abstract::Fat deposition in pigs, which significantly contributes to meat quality, fattening efficiency, reproductive performance, and immunity, is critically affected by preadipocyte adipogenic differentiation. We elucidated adipogenesis in pigs using transcriptome analysis. Preadipocytes from subcutaneous adipose tissue (SAT)...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes10070552

    authors: Zhao X,Chen S,Tan Z,Wang Y,Zhang F,Yang T,Liu Y,Ao H,Xing K,Wang C

    更新日期:2019-07-19 00:00:00

  • Sustentacular Cell Enwrapment of Olfactory Receptor Neuronal Dendrites: An Update.

    abstract::The pseudostratified olfactory epithelium (OE) may histologically appear relatively simple, but the cytological relations among its cell types, especially those between olfactory receptor neurons (ORNs) and olfactory sustentacular cells (OSCs), prove more complex and variable than previously believed. Adding to the co...

    journal_title:Genes

    pub_type: 杂志文章,评审

    doi:10.3390/genes11050493

    authors: Liang F

    更新日期:2020-04-30 00:00:00

  • A Comparative Analysis of CD32A and CD16A Polymorphisms in Relation to Autoimmune Responses in Pemphigus Diseases and Subepithelial Autoimmune Blistering Disorders.

    abstract::Autoimmune blistering dermatoses (ABDs) are characterized by autoantibodies to keratinocyte surface antigens and molecules within the dermal-epidermal junction causing disruption of skin integrity. The affinity of Fc receptors (FcRs) causing an autoimmune response in ABDs may vary based on single-nucleotide polymorphi...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes11040371

    authors: Gornowicz-Porowska J,Kowalczyk MJ,Seraszek-Jaros A,Bowszyc-Dmochowska M,Kaczmarek E,Żaba R,Dmochowski M

    更新日期:2020-03-30 00:00:00

  • Germination-Arrest Bacillus subtilis Spores as An Oral Delivery Vehicle of Grass Carp Reovirus (GCRV) Vp7 Antigen Augment Protective Immunity in Grass Carp (Ctenopharyngodon idella).

    abstract::Oral vaccination is a practical method for the active immunization of farmed fish in the matter of animal welfare and handling costs. However, it always shows insufficient protective immunity, mainly due to antigen degradation in the gastrointestinal tract (GIT). Bacillus subtilis spores have been shown to be able to ...

    journal_title:Genes

    pub_type: 杂志文章

    doi:10.3390/genes11111351

    authors: Sun R,Zhang M,Chen H,Wei Y,Ning D

    更新日期:2020-11-14 00:00:00