Abstract：
:DNA double-strand break (DSB) repair is crucial to maintain genomic stability. The fidelity of the repair depends on the complexity of the lesion, with clustered DSBs being more difficult to repair than isolated breaks. Using live cell imaging of heavy ion tracks produced at a high-energy particle accelerator we visualised simultaneously the recruitment of different proteins at individual sites of complex and simple DSBs in human cells. NBS1 and 53BP1 were recruited in a few seconds to complex DSBs, but in 40% of the isolated DSBs the recruitment was delayed approximately 5 min. Using base excision repair (BER) inhibitors we demonstrate that some simple DSBs are generated by enzymatic processing of base damage, while BER did not affect the complex DSBs. The results show that DSB processing and repair kinetics are dependent on the complexity of the breaks and can be different even for the same clastogenic agent.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Jakob B,Dubiak-Szepietowska M,Janiel E,Schmidt A,Durante M,Taucher-Scholz Gdoi
10.1038/s41598-020-58084-6subject
Has Abstractpub_date
2020-01-29 00:00:00pages
1443issue
1issn
2045-2322pii
10.1038/s41598-020-58084-6journal_volume
10pub_type
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