当前位置： SCI文献检索 > Genes期刊下所有文献 > Off Earth Identification of Bacterial Populations Using 16S rDNA Nanopore Sequencing.

Off Earth Identification of Bacterial Populations Using 16S rDNA Nanopore Sequencing.

Abstract：

:The MinION sequencer has made in situ sequencing feasible in remote locations. Following our initial demonstration of its high performance off planet with Earth-prepared samples, we developed and tested an end-to-end, sample-to-sequencer process that could be conducted entirely aboard the International Space Station (ISS). Initial experiments demonstrated the process with a microbial mock community standard. The DNA was successfully amplified, primers were degraded, and libraries prepared and sequenced. The median percent identities for both datasets were 84%, as assessed from alignment of the mock community. The ability to correctly identify the organisms in the mock community standard was comparable for the sequencing data obtained in flight and on the ground. To validate the process on microbes collected from and cultured aboard the ISS, bacterial cells were selected from a NASA Environmental Health Systems Surface Sample Kit contact slide. The locations of bacterial colonies chosen for identification were labeled, and a small number of cells were directly added as input into the sequencing workflow. Prepared DNA was sequenced, and the data were downlinked to Earth. Return of the contact slide to the ground allowed for standard laboratory processing for bacterial identification. The identifications obtained aboard the ISS, Staphylococcus hominis and Staphylococcus capitis, matched those determined on the ground down to the species level. This marks the first ever identification of microbes entirely off Earth, and this validated process could be used for in-flight microbial identification, diagnosis of infectious disease in a crewmember, and as a research platform for investigators around the world.

journal_name

Genes (Basel)

journal_title

Genes

authors

Burton AS,Stahl SE,John KK,Jain M,Juul S,Turner DJ,Harrington ED,Stoddart D,Paten B,Akeson M,Castro-Wallace SL

doi

10.3390/genes11010076

subject

Has Abstract

pub_date

2020-01-09 00:00:00

issue

1

issn

2073-4425

pii

genes11010076

journal_volume

11

pub_type

杂志文章

相关文献

Genes文献大全
  • Molecular Strategies for RPGR Gene Therapy.

    abstract：:Mutations affecting the Retinitis Pigmentosa GTPase Regulator (RPGR) gene are the commonest cause of X-linked and recessive retinitis pigmentosa (RP), accounting for 10%-20% of all cases of RP. The phenotype is one of the most severe amongst all causes of RP, characteristic for its early onset and rapid progression to...

    journal_title：Genes

    pub_type： 杂志文章,评审

    doi：10.3390/genes10090674

    authors： Cehajic Kapetanovic J,McClements ME,Martinez-Fernandez de la Camara C,MacLaren RE

    更新日期：2019-09-04 00:00:00

  • Biological Regulatory Network (BRN) Analysis and Molecular Docking Simulations to Probe the Modulation of IP3R Mediated Ca2+ Signaling in Cancer.

    abstract：:Inositol trisphosphate receptor (IP3R) mediated Ca+2 signaling is essential in determining the cell fate by regulating numerous cellular processes, including cell division and cell death. Despite extensive studies about the characterization of IP3R in cancer, the underlying molecular mechanism initiating the cell prol...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes12010034

    authors： Ismatullah H,Jabeen I,Saeed MT

    更新日期：2020-12-29 00:00:00

  • Type Three Secretion System in Pseudomonas savastanoi Pathovars: Does Timing Matter?

    abstract：:Pseudomonas savastanoi pv. savastanoi is the causal agent of Olive knot disease, relying on the Type Three Secretion System (TTSS) for its pathogenicity. In this regard, nothing was known about the two other pathovars belonging to this species, pv. nerii and pv. fraxini, characterized by a different host range. Here w...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes2040957

    authors： Tegli S,Gori A,Cerboneschi M,Cipriani MG,Sisto A

    更新日期：2011-11-25 00:00:00

  • Comprehensive History of CSP Genes: Evolution, Phylogenetic Distribution and Functions.

    abstract：:In this review we present the developmental, histological, evolutionary and functional properties of insect chemosensory proteins (CSPs) in insect species. CSPs are small globular proteins folded like a prism and notoriously known for their complex and arguably obscure function(s), particularly in pheromone olfaction....

    journal_title：Genes

    pub_type： 杂志文章,评审

    doi：10.3390/genes11040413

    authors： Liu G,Xuan N,Rajashekar B,Arnaud P,Offmann B,Picimbon JF

    更新日期：2020-04-10 00:00:00

  • Investigation of Precise Molecular Mechanistic Action of Tobacco-Associated Carcinogen `NNK´ Induced Carcinogenesis: A System Biology Approach.

    abstract：:Cancer is the second deadliest disease listed by the WHO. One of the major causes of cancer disease is tobacco and consumption possibly due to its main component, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). A plethora of studies have been conducted in the past aiming to decipher the association of NNK with o...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes10080564

    authors： Anukriti.,Dhasmana A,Uniyal S,Somvanshi P,Bhardwaj U,Gupta M,Haque S,Lohani M,Kumar D,Ruokolainen J,Kesari KK

    更新日期：2019-07-26 00:00:00

  • Pipeline for the Rapid Development of Cytogenetic Markers Using Genomic Data of Related Species.

    abstract：:Repetitive DNA including tandem repeats (TRs) is a significant part of most eukaryotic genomes. TRs include rapidly evolving satellite DNA (satDNA) that can be shared by closely related species, their abundance may be associated with evolutionary divergence, and they have been widely used for chromosome karyotyping us...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes10020113

    authors： Kroupin P,Kuznetsova V,Romanov D,Kocheshkova A,Karlov G,Dang TX,Khuat TML,Kirov I,Alexandrov O,Polkhovskiy A,Razumova O,Divashuk M

    更新日期：2019-02-01 00:00:00

  • The Genome Assembly and Annotation of the Southern Elephant Seal Mirounga leonina.

    abstract：:The southern elephant seal Mirounga leonina is the largest phocid seal and one of the two species of elephant seals. They are listed as 'least concern' by the International Union for Conservation of Nature (IUCN) Red List of Threatened Species 2015. Here, we have assembled the reference genome for M. leonina using the...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes11020160

    authors： Kim BM,Lee YJ,Kim JH,Kim JH,Kang S,Jo E,Lee SJ,Lee JH,Chi YM,Park H

    更新日期：2020-02-03 00:00:00

  • Coat Color Roan Shows Association with KIT Variants and No Evidence of Lethality in Icelandic Horses.

    abstract：:Roan (Rn) horses show a typical seasonal change of color. Their body is covered with colored and white hair. We performed a descriptive statistical analysis of breeding records of Icelandic horses to challenge the hypothesis of roan being lethal in utero under homozygous condition. The roan to non-roan ratio of foals ...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes11060680

    authors： Voß K,Tetens J,Thaller G,Becker D

    更新日期：2020-06-22 00:00:00

  • Sequence Composition Underlying Centromeric and Heterochromatic Genome Compartments of the Pacific Oyster Crassostrea gigas.

    abstract：:Segments of the genome enriched in repetitive sequences still present a challenge and are omitted in genome assemblies. For that reason, the exact composition of DNA sequences underlying the heterochromatic regions and the active centromeres are still unexplored for many organisms. The centromere is a crucial region o...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes11060695

    authors： Tunjić Cvitanić M,Vojvoda Zeljko T,Pasantes JJ,García-Souto D,Gržan T,Despot-Slade E,Plohl M,Šatović E

    更新日期：2020-06-24 00:00:00

  • Transcriptional Structure of Petunia Clock in Leaves and Petals.

    abstract：:The plant circadian clock coordinates environmental signals with internal processes including secondary metabolism, growth, flowering, and volatile emission. Plant tissues are specialized in different functions, and petals conceal the sexual organs while attracting pollinators. Here we analyzed the transcriptional str...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes10110860

    authors： Terry MI,Carrera-Alesina M,Weiss J,Egea-Cortines M

    更新日期：2019-10-30 00:00:00

  • Analyses of Hybrid Viability across a Hybrid Zone between Two Alnus Species Using Microsatellites and cpDNA Markers.

    abstract：:Diploid Alnus glutinosa s. str. and autotetraploid A. rohlenae form a narrow hybrid zone in a study area in southern Serbia, which results in triploid hybrid formation. The vast majority of previous studies have been focused on studies of maternal plants, but the offspring resulting from their crossing have not been m...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes11070770

    authors： Šmíd J,Douda J,Krak K,Mandák B

    更新日期：2020-07-09 00:00:00

  • Study of the Relationship between Polymorphisms in the IL-8 Gene Promoter Region and Coccidiosis Resistance Index in Jinghai Yellow Chickens.

    abstract：:Interleukin 8 (IL-8) participates in the immune response and has the function of inducing neutrophils to release lysosomal enzymes and eliminate pathogens. This study was to investigate the effect of single nucleotide mutations in the IL-8 gene promoter region on the coccidiosis resistance index. In this study, 180 in...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes11050476

    authors： Wang XH,Yu HL,Zou WB,Mi CH,Dai GJ,Zhang T,Zhang GX,Xie KZ,Wang JY

    更新日期：2020-04-27 00:00:00

  • Evolutionary Insights of the ZW Sex Chromosomesin Snakes: A New Chapter Added by the AmazonianPuffing Snakes of the Genus Spilotes.

    abstract：:Amazonian puffing snakes (Spilotes; Colubridae) are snakes widely distributed in the Neotropical region. However, chromosomal data are scarce in this group and, when available, are only limited to karyotype description using conventional staining. In this paper, we focused on the process of karyotype evolution and tre...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes10040288

    authors： Viana PF,Ezaz T,de Bello Cioffi M,Jackson Almeida B,Feldberg E

    更新日期：2019-04-09 00:00:00

  • Exposure to Formaldehyde Perturbs the Mouse Gut Microbiome.

    abstract：:Exposure to Formaldehyde (FA) results in many pathophysiological symptoms, however the underlying mechanisms are not well understood. Given the complicated modulatory role of intestinal microbiota on human health, we hypothesized that interactions between FA and the gut microbiome may account for FA's toxicity. Balb/c...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes9040192

    authors： Guo J,Zhao Y,Jiang X,Li R,Xie H,Ge L,Xie B,Yang X,Zhang L

    更新日期：2018-04-03 00:00:00

  • Genome-Wide Identification and Expression Profiling of Cytokinin Oxidase/Dehydrogenase (CKX) Genes Reveal Likely Roles in Pod Development and Stress Responses in Oilseed Rape (Brassica napus L.).

    abstract：:Cytokinin oxidase/dehydrogenases (CKXs) play a critical role in the irreversible degradation of cytokinins, thereby regulating plant growth and development. Brassica napus is one of the most widely cultivated oilseed crops worldwide. With the completion of whole-genome sequencing of B. napus, genome-wide identificatio...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes9030168

    authors： Liu P,Zhang C,Ma JQ,Zhang LY,Yang B,Tang XY,Huang L,Zhou XT,Lu K,Li JN

    更新日期：2018-03-16 00:00:00

  • Efficient Generation and Correction of Mutations in Human iPS Cells Utilizing mRNAs of CRISPR Base Editors and Prime Editors.

    abstract：:In contrast to CRISPR/Cas9 nucleases, CRISPR base editors (BE) and prime editors (PE) enable predefined nucleotide exchanges in genomic sequences without generating DNA double strand breaks. Here, we employed BE and PE mRNAs in conjunction with chemically synthesized sgRNAs and pegRNAs for efficient editing of human i...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes11050511

    authors： Sürün D,Schneider A,Mircetic J,Neumann K,Lansing F,Paszkowski-Rogacz M,Hänchen V,Lee-Kirsch MA,Buchholz F

    更新日期：2020-05-06 00:00:00

  • Aberrant Single Exon Skipping is not Altered by Age in Exons of NF1, RABAC1, AATF or PCGF2 in Human Blood Cells and Fibroblasts.

    abstract：:In human pre-mRNA splicing, infrequent errors occur resulting in erroneous splice products as shown in a genome-wide approach. One characteristic subgroup consists of products lacking one cassette exon. The noise in the splicing process, represented by those misspliced products, can be increased by cold shock treatmen...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes2030562

    authors： Mellert K,Uhl M,Högel J,Lamla M,Kemkemer R,Kaufmann D

    更新日期：2011-08-02 00:00:00

  • The Chloroplast Genome Sequence of Scutellaria baicalensis Provides Insight into Intraspecific and Interspecific Chloroplast Genome Diversity in Scutellaria.

    abstract：:Scutellaria baicalensis Georgi (Lamiaceae) is the source of the well-known traditional Chinese medicine "HuangQin" (Radix Scutellariae). Natural sources of S. baicalensis are rapidly declining due to high market demand and overexploitation. Moreover, the commercial products of Radix Scutellariae have often been found ...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes8090227

    authors： Jiang D,Zhao Z,Zhang T,Zhong W,Liu C,Yuan Q,Huang L

    更新日期：2017-09-13 00:00:00

  • Specific LTR-Retrotransposons Show Copy Number Variations between Wild and Cultivated Sunflowers.

    abstract：:The relationship between variation of the repetitive component of the genome and domestication in plant species is not fully understood. In previous work, variations in the abundance and proximity to genes of long terminal repeats (LTR)-retrotransposons of sunflower (Helianthus annuus L.) were investigated by Illumina...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes9090433

    authors： Mascagni F,Vangelisti A,Giordani T,Cavallini A,Natali L

    更新日期：2018-08-29 00:00:00

  • A Third MLPH Variant Causing Coat Color Dilution in Dogs.

    abstract：:Altered melanosome transport in melanocytes, resulting from variants in the melanophilin (MLPH) gene, are associated with inherited forms of coat color dilution in many species. In dogs, the MLPH gene corresponds to the D locus and two variants, c.-22G > A (d1) and c.705G > C (d2), leading to the dilution of coat colo...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes11060639

    authors： Van Buren SL,Minor KM,Grahn RA,Mickelson JR,Grahn JC,Malvick J,Colangelo JR,Mueller E,Kuehnlein P,Kehl A

    更新日期：2020-06-10 00:00:00

  • PupStruct: Prediction of Pupylated Lysine Residues Using Structural Properties of Amino Acids.

    abstract：:Post-translational modification (PTM) is a critical biological reaction which adds to the diversification of the proteome. With numerous known modifications being studied, pupylation has gained focus in the scientific community due to its significant role in regulating biological processes. The traditional experimenta...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes11121431

    authors： Singh V,Sharma A,Dehzangi A,Tsunoda T

    更新日期：2020-11-28 00:00:00

  • Plateau Grass and Greenhouse Flower? Distinct Genetic Basis of Closely Related Toad Tadpoles Respectively Adapted to High Altitude and Karst Caves.

    abstract：:Genetic adaptation to extremes is a fascinating topic. Nevertheless, few studies have explored the genetic adaptation of closely related species respectively inhabiting distinct extremes. With deep transcriptome sequencing, we attempt to detect the genetic architectures of tadpoles of five closely related toad species...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes11020123

    authors： Chang L,Zhu W,Shi S,Zhang M,Jiang J,Li C,Xie F,Wang B

    更新日期：2020-01-22 00:00:00

  • Functional Haplotype of LIPC Induces Triglyceride-Mediated Suppression of HDL-C Levels According to Genome-Wide Association Studies.

    abstract：:Hepatic lipase (encoded by LIPC) is a glycoprotein in the triacylglycerol lipase family and mainly synthesized in and secreted from the liver. Previous studies demonstrated that hepatic lipase is crucial for reverse cholesterol transport and modulating metabolism and the plasma levels of several lipoproteins. This stu...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes12020148

    authors： Liao YH,Er LK,Wu S,Ko YL,Teng MS

    更新日期：2021-01-22 00:00:00

  • CYP6P9-Driven Signatures of Selective Sweep of Metabolic Resistance to Pyrethroids in the Malaria Vector Anopheles funestus Reveal Contemporary Barriers to Gene Flow.

    abstract：:Pyrethroid resistance in major malaria vectors such as Anopheles funestus threatens malaria control efforts in Africa. Cytochrome P450-mediated metabolic resistance is best understood for CYP6P9 genes in southern Africa in An. funestus. However, we do not know if this resistance mechanism is spreading across Africa an...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes11111314

    authors： Djuicy DD,Hearn J,Tchouakui M,Wondji MJ,Irving H,Okumu FO,Wondji CS

    更新日期：2020-11-05 00:00:00

  • Whether Gametophytes are Reduced or Unreduced in Angiosperms Might Be Determined Metabolically.

    abstract：:In angiosperms, meiotic failure coupled with the formation of genetically unreduced gametophytes in ovules (apomeiosis) constitute major components of gametophytic apomixis. These aberrant developmental events are generally thought to be caused by mutation. However, efforts to locate the responsible mutations have fai...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes11121449

    authors： Mateo de Arias M,Gao L,Sherwood DA,Dwivedi KK,Price BJ,Jamison M,Kowallis BM,Carman JG

    更新日期：2020-12-02 00:00:00

  • Cryptic Diversity Hidden within the Leafminer Genus Liriomyza (Diptera: Agromyzidae).

    abstract：:Leafminer insects of the genus Liriomyza are small flies whose larvae feed on the internal tissue of some of the most important crop plants for the human diet. Several of these pest species are highly uniform from the morphological point of view, meaning molecular data represents the only reliable taxonomic tool usefu...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes9110554

    authors： Carapelli A,Soltani A,Leo C,Vitale M,Amri M,Mediouni-Ben Jemâa J

    更新日期：2018-11-15 00:00:00

  • The Complete Genome of an Endogenous Nimavirus (Nimav-1_LVa) From the Pacific Whiteleg Shrimp Penaeus (Litopenaeus) Vannamei.

    abstract：:White spot syndrome virus (WSSV), the lone virus of the genus Whispovirus under the family Nimaviridae, is one of the most devastating viruses affecting the shrimp farming industry. Knowledge about this virus, in particular, its evolution history, has been limited, partly due to its large genome and the lack of other ...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes11010094

    authors： Bao W,Tang KFJ,Alcivar-Warren A

    更新日期：2020-01-14 00:00:00

  • Transcriptome Analysis of Landrace Pig Subcutaneous Preadipocytes during Adipogenic Differentiation.

    abstract：:Fat deposition in pigs, which significantly contributes to meat quality, fattening efficiency, reproductive performance, and immunity, is critically affected by preadipocyte adipogenic differentiation. We elucidated adipogenesis in pigs using transcriptome analysis. Preadipocytes from subcutaneous adipose tissue (SAT)...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes10070552

    authors： Zhao X,Chen S,Tan Z,Wang Y,Zhang F,Yang T,Liu Y,Ao H,Xing K,Wang C

    更新日期：2019-07-19 00:00:00

  • Characterization of Differentially Expressed miRNAs and Their Predicted Target Transcripts during Smoltification and Adaptation to Seawater in Head Kidney of Atlantic Salmon.

    abstract：:Smoltification and early seawater phase are critical developmental periods with physiological and biochemical changes in Atlantic salmon that facilitates survival in saltwater. MicroRNAs (miRNAs) are known to have important roles in development, but whether any miRNAs are involved in regulation of gene expression duri...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes11091059

    authors： Shwe A,Østbye TK,Krasnov A,Ramberg S,Andreassen R

    更新日期：2020-09-08 00:00:00

  • TERribly Difficult: Searching for Telomerase RNAs in Saccharomycetes.

    abstract：:The telomerase RNA in yeasts is large, usually >1000 nt, and contains functional elements that have been extensively studied experimentally in several disparate species. Nevertheless, they are very difficult to detect by homology-based methods and so far have escaped annotation in the majority of the genomes of Saccha...

    journal_title：Genes

    pub_type： 杂志文章

    doi：10.3390/genes9080372

    authors： Waldl M,Thiel BC,Ochsenreiter R,Holzenleiter A,de Araujo Oliveira JV,Walter MEMT,Wolfinger MT,Stadler PF

    更新日期：2018-07-26 00:00:00