Abstract：
:Fat deposition in pigs, which significantly contributes to meat quality, fattening efficiency, reproductive performance, and immunity, is critically affected by preadipocyte adipogenic differentiation. We elucidated adipogenesis in pigs using transcriptome analysis. Preadipocytes from subcutaneous adipose tissue (SAT) of Landrace piglets were differentiated into adipocytes in vitro. RNA sequencing (RNA-seq) used to screen differentially expressed genes (DEGs) during preadipocyte differentiation up to day 8 revealed 15,918 known and 586 novel genes. We detected 21, 144, and 394 DEGs, respectively, including 16 genes differentially expressed at days 2, 4 and 8 compared to day 0. Th number of DEGs increased time-dependently. Lipid metabolism, cell differentiation and proliferation, peroxisome proliferator-activated receptor (PPAR), wingless-type MMTV integration site (Wnt), tumor necrosis factor (TNF) signaling, and steroid biosynthesis were significant at days 2, 4, and 8 compared to day 0 (adjusted p < 0.05). Short time-series expression miner (STEM) analysis obtained 26 clusters of differential gene expression patterns, and nine were significant (p < 0.05). Functional analysis showed many significantly enriched lipid deposition- and cellular process-related biological processes and pathways in profiles 9, 21, 22, and 24. Glycerolipid and fatty-acid metabolism, PPAR signaling, fatty-acid degradation, phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt), and TNF signaling were observed during preadipocyte differentiation in vitro. These findings will facilitate the comprehension of preadipocyte differentiation and fat deposition in pigs.
journal_name
Genes (Basel)journal_title
Genesauthors
Zhao X,Chen S,Tan Z,Wang Y,Zhang F,Yang T,Liu Y,Ao H,Xing K,Wang Cdoi
10.3390/genes10070552subject
Has Abstractpub_date
2019-07-19 00:00:00issue
7issn
2073-4425pii
genes10070552journal_volume
10pub_type
杂志文章相关文献
Genes文献大全abstract：:B-cell acute lymphoblastic leukemia is the most commonly diagnosed childhood malignancy worldwide; more than 50% of these cases are diagnosed in Mexico. Although the five-year survival rate is >80%, 30% of patients experience relapse with poor prognosis. Cancer-associated gene expression profiles have been identified ...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes10090716
更新日期：2019-09-16 00:00:00
abstract：BACKGROUND:Engineering apomixis in sexually reproducing plants has been long desired because of the potential to fix hybrid vigor. Validating the functionality of genes originated from apomictic species that contribute to apomixis upon transfer to sexually reproducing species is an important step. The PsASGR-BABYBOOM-l...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11091072
更新日期：2020-09-12 00:00:00
abstract：:Eimeria tenella (E. tenella) is one of the most frequent and pathogenic species of protozoan parasites of the genus Eimeria that exclusively occupies the cecum, exerting a high economic impact on the poultry industry. To investigate differentially expressed genes (DEGs) in the cecal tissue of Jinghai yellow chickens i...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes10060420
更新日期：2019-05-31 00:00:00
abstract：:White coat color in mammals has been selected several times during the domestication process. Numerous dog breeds are fixed for one form of white coat color that involves darkly pigmented skin. The genetic basis of this color, due to the absence of pigment in the hairs, was suggested to correspond to extreme dilution ...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes10050386
更新日期：2019-05-21 00:00:00
abstract：:Lactobacillus ruminis is a commensal motile lactic acid bacterium living in the intestinal tract of humans and animals. Although a few genomes of L. ruminis were published, most of them were animal derived. To explore the genetic diversity and potential niche-specific adaptation changes of L. ruminis, in the current w...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11010070
更新日期：2020-01-08 00:00:00
abstract：:The availability of the genome sequence of the unisexual (male-female) Caenorhabditis nigoni offers an opportunity to compare its non-coding features with the related hermaphroditic species Caenorhabditis briggsae; to understand the evolutionary dynamics of their tandem repeat sequences (satellites), as a result of ev...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes8120351
更新日期：2017-11-28 00:00:00
abstract：:Although a number of methods have been proposed for identifying differentially expressed pathways (DEPs), few efforts consider the dynamic components of pathway networks, i.e., gene links. We here propose a signaling dynamics detection method for identification of DEPs, DynSig, which detects the molecular signaling ch...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes9070323
更新日期：2018-06-27 00:00:00
abstract：:Balanced chromosome rearrangements are one of the main etiological factors contributing to hypoprolificacy in the domestic pig. Amongst domestic animals, the pig is considered to have the highest prevalence of chromosome rearrangements. To date over 200 unique chromosome rearrangements have been identified. The factor...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes10100769
更新日期：2019-09-30 00:00:00
abstract：:Grouper is one of the favorite sea food resources in Southeast Asia. However, the outbreaks of the viral nervous necrosis (VNN) disease due to nervous necrosis virus (NNV) infection have caused mass mortality of grouper larvae. Many aqua-farms have suffered substantial financial loss due to the occurrence of VNN. To b...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes8010031
更新日期：2017-01-13 00:00:00
abstract：:Smoltification and early seawater phase are critical developmental periods with physiological and biochemical changes in Atlantic salmon that facilitates survival in saltwater. MicroRNAs (miRNAs) are known to have important roles in development, but whether any miRNAs are involved in regulation of gene expression duri...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11091059
更新日期：2020-09-08 00:00:00
abstract：:Intercellular karyotypic variability has been a focus of genetic research for more than 50 years. It has been repeatedly shown that chromosome heterogeneity manifesting as chromosomal mosaicism is associated with a variety of human diseases. Due to the ability of changing dynamically throughout the ontogeny, chromosom...
journal_title：Genes
pub_type： 杂志文章,评审
doi：10.3390/genes10050379
更新日期：2019-05-19 00:00:00
abstract：:Long non-coding (lnc) RNAs serve a multitude of functions in cells. NEAT1 RNA is a highly abundant 4 kb lncRNA in nuclei, and coincides with paraspeckles, nuclear domains that control sequestration of paraspeckle proteins. We examined NEAT1 RNA levels and its function in 3T3-L1 cells during differentiation to adipocyt...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes5041050
更新日期：2014-11-27 00:00:00
abstract：:Due to its extraordinary longevity and wide distribution, the ocean quahog Arctica islandica has become an important species model in both aging and environmental change research. Notwithstanding that, most genetic studies on ocean quahogs have been focused on fishery related, phylogeographic and phylogenetic aspects ...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes9060299
更新日期：2018-06-13 00:00:00
abstract：:The northern sea otter inhabits coastal waters of the northern Pacific Ocean and is the largest member of the Mustelidae family. DNA sequencing methods that utilize microfluidic partitioned and non-partitioned library construction were used to establish the sea otter genome. The final assembly provided 2.426 Gbp of hi...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes8120379
更新日期：2017-12-11 00:00:00
abstract：:During the evolution of heteromorphic sex chromosomes, the sex-specific Y chromosome degenerates, while the X chromosome evolves new mechanisms of regulation. Using bioinformatic and experimental approaches, we investigate the expression of the X chromosome in Drosophila melanogaster. We observe nearly complete X chro...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes9050242
更新日期：2018-05-04 00:00:00
abstract：:White mold (WM) is a devastating fungal disease affecting common bean (Phaseolus vulgaris L.). In this research, a genome-wide association study (GWAS) for WM resistance was conducted using 294 lines of the Spanish diversity panel. One single-locus method and six multi-locus methods were used in the GWAS. Response to ...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11121496
更新日期：2020-12-12 00:00:00
abstract：:Inherited retinal degenerations are the leading cause of blindness in the working population. X-linked retinitis pigmentosa (XLRP), caused by mutations in the Retinitis pigmentosa GTPase regulator (RPGR) gene is one of the more severe forms, and female carriers of RPGR mutations have a variable presentation. A retrosp...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes9120643
更新日期：2018-12-18 00:00:00
abstract：:Mitochondrial dynamics are known to have an important role in so-called age-related diseases, including cancer. Mitochondria is an organelle involved in many key cellular functions and responds to physiologic or stress stimuli by adapting its structure and function. Perhaps the most important structural changes involv...
journal_title：Genes
pub_type： 杂志文章,评审
doi：10.3390/genes9020115
更新日期：2018-02-21 00:00:00
abstract：:This review discusses a set of experimental results that support the existence of extended strand displacement events during budding yeast lagging strand DNA synthesis. Starting from introducing the mechanisms and factors involved in leading and lagging strand DNA synthesis and some aspects of the architecture of the ...
journal_title：Genes
pub_type： 杂志文章,评审
doi：10.3390/genes10020167
更新日期：2019-02-21 00:00:00
abstract：:Genetic adaptation to extremes is a fascinating topic. Nevertheless, few studies have explored the genetic adaptation of closely related species respectively inhabiting distinct extremes. With deep transcriptome sequencing, we attempt to detect the genetic architectures of tadpoles of five closely related toad species...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11020123
更新日期：2020-01-22 00:00:00
abstract：:The wall-associated kinase (WAK) and wall-associated kinase like (WAKL) is a subfamily of receptor-like kinases associated with the cell wall, which have been suggested as sensors of the extracellular environment and triggers of intracellular signals. However, these proteins have not yet been comprehensively analyzed ...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11101186
更新日期：2020-10-12 00:00:00
abstract：:High-throughput technologies have allowed researchers to obtain genome-wide data from a wide array of experimental model systems. Unfortunately, however, new data generation tends to significantly outpace data re-utilization, and most high throughput datasets are only rarely used in subsequent studies or to generate n...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes10060423
更新日期：2019-06-01 00:00:00
abstract：:The conserved Notch pathway is reported to be involved in progesterone synthesis and secretion; however, the exact effects remain controversial. To determine the role and potential mechanisms of the Notch signaling pathway in progesterone biosynthesis in porcine granulosa cells (pGCs), we first used a pharmacological ...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11020120
更新日期：2020-01-22 00:00:00
abstract：:The scale of genetic methods are presently being expanded: forensic genetic assays previously were limited to tens of loci, but now technologies allow for a transition to forensic genomic approaches that assess thousands to millions of loci. However, there are subtle distinctions between genetic assays and their genom...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes12020185
更新日期：2021-01-27 00:00:00
abstract：:Acute myeloid leukemia (AML) can develop as a secondary malignancy following radiotherapy, but also following low-dose environmental or occupational radiation exposure. Therapy-related AML frequently carries deletions of chromosome 5q and/or 7, but for low-dose exposure associated AML this has not been described. For ...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes2020384
更新日期：2011-05-31 00:00:00
abstract：:Crested wheatgrass (Agropyron cristatum L.) breeding programs aim to develop later maturing cultivars for extending early spring grazing in Western Canada. Plant maturity is a complex genetic trait, and little is known about genes associated with late maturity in this species. An attempt was made using RNA-Seq to prof...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes8110291
更新日期：2017-10-25 00:00:00
abstract：:Shiga toxin-producing Escherichia coli (STEC) are foodborne pathogens associated with outbreaks and hemolytic-uremic syndrome. Cattle and meat foods are the main reservoir and infection source, respectively. Pathogenicity islands (PAIs) play an important role in STEC pathogenicity, and non-locus of the enterocyte effa...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes9020081
更新日期：2018-02-10 00:00:00
abstract：:The relationship between variation of the repetitive component of the genome and domestication in plant species is not fully understood. In previous work, variations in the abundance and proximity to genes of long terminal repeats (LTR)-retrotransposons of sunflower (Helianthus annuus L.) were investigated by Illumina...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes9090433
更新日期：2018-08-29 00:00:00
abstract：:The nitrogen use efficiency (NUE) of crop plants is limited and enhancing it in rice, a major cereal crop, would be beneficial for farmers and the environment alike. Here we report the genome-wide transcriptome analysis of two rice genotypes, IR 64 (IR64) and Nagina 22 (N22) under optimal (+N) and chronic starvation (...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes9040206
更新日期：2018-04-11 00:00:00
abstract：:Network medicine relies on different types of networks: from the molecular level of protein⁻protein interactions to gene regulatory network and correlation studies of gene expression. Among network approaches based on the analysis of the topological properties of protein⁻protein interaction (PPI) networks, we discuss ...
journal_title：Genes
pub_type： 杂志文章,评审
doi：10.3390/genes9090437
更新日期：2018-08-31 00:00:00
