Abstract:
BACKGROUND:Engineering apomixis in sexually reproducing plants has been long desired because of the potential to fix hybrid vigor. Validating the functionality of genes originated from apomictic species that contribute to apomixis upon transfer to sexually reproducing species is an important step. The PsASGR-BABYBOOM-like (PsASGR-BBML) gene from Pennisetum squamulatum confers parthenogenesis in this apomict, and its functionality was demonstrated in several sexually reproducing monocots but not in any dicots. METHODS:We introduced the PsASGR-BBML gene regulated by egg cell-specific promoters, either AtDD45 or AtRKD2, into tobacco, and analyzed progeny of the transgenic lines resulting from self-pollination and crossing by flow cytometry. RESULTS:We identified haploid progeny at a frequency lower than 1% in the AtDD45pro lines, while at a frequency of 9.3% for an octoploid (2n = 8x) AtRKD2pro line. Haploid production in the T2 generation, derived from the tetraploid T1 offspring of this original octoploid AtRKD2pro line, was also observed. Pollinated by homozygous transgenic tobacco carrying a DsRed marker gene, 4x progeny of the AtRKD2pro line yielded parthenogenetic embryos identified as DsRed negative. We verified that the DsRed negative seedlings recovered were haploid (2x). CONCLUSION:The PsASGR-BBML gene regulated by egg cell-specific promoters could enable parthenogenesis in tobacco, a dicotyledon species.
journal_name
Genes (Basel)journal_title
Genesauthors
Zhang Z,Conner J,Guo Y,Ozias-Akins Pdoi
10.3390/genes11091072subject
Has Abstractpub_date
2020-09-12 00:00:00issue
9issn
2073-4425pii
genes11091072journal_volume
11pub_type
杂志文章相关文献
Genes文献大全abstract::Despite the extensive research conducted in recent decades, the molecular mechanisms underlying major depressive disorder (MDD) and relative evidence-based treatments remain unclear. Various hypotheses have been successively proposed, involving different biological systems. This narrative review aims to critically ill...
journal_title:Genes
pub_type: 杂志文章,评审
doi:10.3390/genes11091089
更新日期:2020-09-18 00:00:00
abstract::Horses have been studied for exercise function rather than food production, unlike most livestock. Therefore, the role and characteristics of tissue landscapes are critically understudied, except for certain muscles used in exercise-related studies. In the present study, we compared RNA-Seq data from 18 Jeju horse ske...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11111359
更新日期:2020-11-17 00:00:00
abstract::Although genome-wide association studies (GWAS) have identified several hundred loci associated with autoimmune diseases, their mechanistic insights are still poorly understood. The human genome is more complex than single nucleotide polymorphisms (SNPs) that are interrogated by GWAS arrays. Apart from SNPs, it also c...
journal_title:Genes
pub_type: 杂志文章,评审
doi:10.3390/genes9080377
更新日期:2018-07-27 00:00:00
abstract::Biallelic PIGT variants were previously reported in seven patients from three families with Multiple Congenital Anomalies-Hypotonia Seizures Syndrome 3 (MCAHS3), characterized by epileptic encephalopathy, hypotonia, global developmental delay/intellectual disability, cerebral and cerebellar atrophy, craniofacial dysmo...
journal_title:Genes
pub_type:
doi:10.3390/genes7120108
更新日期:2016-11-29 00:00:00
abstract::The conserved Notch pathway is reported to be involved in progesterone synthesis and secretion; however, the exact effects remain controversial. To determine the role and potential mechanisms of the Notch signaling pathway in progesterone biosynthesis in porcine granulosa cells (pGCs), we first used a pharmacological ...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11020120
更新日期:2020-01-22 00:00:00
abstract::Satellite DNAs are tandemly repeated sequences preferentially assembled into large arrays within constitutive heterochromatin and their transcription is often activated by stress conditions, particularly by heat stress. Bioinformatic analyses of sequenced genomes however reveal single repeats or short arrays of satell...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11060663
更新日期:2020-06-18 00:00:00
abstract::The sea lamprey (Petromyzon marinus) is one of few vertebrate species known to reproducibly eliminate large fractions of its genome during normal embryonic development. This germline-specific DNA is lost in the form of large fragments, including entire chromosomes, and available evidence suggests that DNA elimination ...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes10100832
更新日期:2019-10-22 00:00:00
abstract::The southern elephant seal Mirounga leonina is the largest phocid seal and one of the two species of elephant seals. They are listed as 'least concern' by the International Union for Conservation of Nature (IUCN) Red List of Threatened Species 2015. Here, we have assembled the reference genome for M. leonina using the...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11020160
更新日期:2020-02-03 00:00:00
abstract::GOLDEN2-LIKE (GLK) is a member of the myeloblastosis (MYB) family transcription factor and it plays an important role in the regulation of plastid development and stress tolerance. In this study, a gene named AhGLK1b was identified from a cultivated peanut showing down-regulation in response to low calcium with a comp...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11030343
更新日期:2020-03-24 00:00:00
abstract::Cell Division Autoantigen 1 (CDA1) was discovered following screening a human expression library with serum from a patient with Discoid Lupus Erythematosus. CDA1, encoded by TSPYL2 on the X chromosome, shares anti-proliferative and pro‑fibrotic properties with TGF-b. It inhibits cell growth through p53, pERK1/2 and p2...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes1030335
更新日期:2010-09-30 00:00:00
abstract::Inositol trisphosphate receptor (IP3R) mediated Ca+2 signaling is essential in determining the cell fate by regulating numerous cellular processes, including cell division and cell death. Despite extensive studies about the characterization of IP3R in cancer, the underlying molecular mechanism initiating the cell prol...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes12010034
更新日期:2020-12-29 00:00:00
abstract::Juvenile open angle glaucoma (JOAG), which is an uncommon form of primary open angle glaucoma, is a clinically and genetically heterogeneous disorder. We report on a family with a recessively inherited form of JOAG. The proband has a superior and an inferior never fiber layer thinning in both the eyes and the nasal vi...
journal_title:Genes
pub_type:
doi:10.3390/genes9110527
更新日期:2018-10-30 00:00:00
abstract::Bacterial fruit blotch (BFB) causes losses in melon marketable yield. However, until now, there has been no information about the genetic loci responsible for resistance to the disease or their pattern of inheritance. We determined the inheritance pattern of BFB resistance from a segregating population of 491 F2 indiv...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11020220
更新日期:2020-02-19 00:00:00
abstract::Preimplantation genetic testing (PGT) has evolved into a well-established alternative to invasive prenatal diagnosis, even though genetic testing of single or few cells is quite challenging. PGT-M is in theory available for any monogenic disorder for which the disease-causing locus has been unequivocally identified. I...
journal_title:Genes
pub_type: 杂志文章,评审
doi:10.3390/genes11080871
更新日期:2020-07-31 00:00:00
abstract::The study of DNA to predict externally visible characteristics (EVCs) and the biogeographical ancestry (BGA) from unknown samples is gaining relevance in forensic genetics. Technical developments in Massively Parallel Sequencing (MPS) enable the simultaneous analysis of hundreds of DNA markers, which improves successf...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11060708
更新日期:2020-06-26 00:00:00
abstract::Illumina and nanopore sequencing technologies are powerful tools that can be used to determine the bacterial composition of complex microbial communities. In this study, we compared nasal microbiota results at genus level using both Illumina and nanopore 16S rRNA gene sequencing. We also monitored the progression of n...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11091105
更新日期:2020-09-21 00:00:00
abstract::Divergent selection between ecologically dissimilar habitats promotes local adaptation, which can lead to reproductive isolation (RI). Populations in the Poecilia mexicana species complex have independently adapted to toxic hydrogen sulfide and show varying degrees of RI. Here, we examined the variation in the mate ch...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes9050232
更新日期:2018-05-02 00:00:00
abstract::Small RNAs play an important role in regulating plant responses to abiotic stress. Depending on the method of salt application, whether sudden or gradual, plants may experience either salt shock or salt stress, respectively. In this study, small RNA expression in response to salt shock and long-term salt stress in par...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes8120369
更新日期:2017-12-05 00:00:00
abstract::Brycon is an important group of Neotropical fish and the principal genus of the family Bryconidae, with 44 valid species that are found in some Central American rivers and practically all the major hydrographic basins of South America. These fish are medium to large in size, migratory, omnivorous, important seed dispe...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes10090639
更新日期:2019-08-23 00:00:00
abstract::Olfaction, which is mediated by olfactory receptor (OR) genes, is essential in the daily life of fish, especially in foraging. However, Chinese perch (Siniperca chuatsi) is believed to prey with reliance on vision and lateral sensation, but not on olfaction. Therefore, understanding the evolutionary dynamics of the Ch...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes10020178
更新日期:2019-02-25 00:00:00
abstract::PPARGC1A rs8192678 G/A (Gly482Ser) and NRF1 rs6949152 A/G polymorphisms have been associated with endurance athlete status, endurance performance phenotypes, and certain health-related markers of different pathologies such as metabolic syndrome, diabetes, and dyslipidemia. We hypothesized that they could be considered...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11091012
更新日期:2020-08-27 00:00:00
abstract::Trees and their seeds regulate their germination, growth, and reproduction in response to environmental stimuli. These stimuli, through signal transduction, trigger transcription factors that alter the expression of various genes leading to the unfolding of the genetic program. A regulon is conceptually defined as a s...
journal_title:Genes
pub_type: 杂志文章,评审
doi:10.3390/genes11090995
更新日期:2020-08-25 00:00:00
abstract::Salinity is one of the major environment factors that limits the growth of plants and the productivity of crops worldwide. It has been shown that Na+ transporters play a central role in salt tolerance and development of plants. The objective of this study was to identify Na+/H+ antiporter (NHX) genes and investigate t...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes10050401
更新日期:2019-05-27 00:00:00
abstract::Drosophila melanogaster is one of the most extensively used genetic model organisms for studying LTR retrotransposons that are represented by various groups in its genome. However, the phenomenon of molecular domestication of LTR retrotransposons has been insufficiently studied in Drosophila, as well as in other inver...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11040396
更新日期:2020-04-06 00:00:00
abstract::The microRNA (miR)-17 family is widely expressed in mammalian tissues and play important roles in various physiological and pathological processes. Here, the functions of miR-106a-5p, a member of miR-17 family, were explored during myogenic differentiation in C2C12 cell line. First, miR-106a-5p was found to be relativ...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes9070333
更新日期:2018-07-02 00:00:00
abstract::Pulmonary hypertension (PH) is a serious disorder with high morbidity and mortality rate. We analyzed the right-ventricular systolic pressure (RVSP), right-ventricular hypertrophy (RVH), lung histology, and transcriptomes of six-week-old male rats with PH induced by (1) hypoxia (HO), (2) administration of monocrotalin...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11020126
更新日期:2020-01-23 00:00:00
abstract:BACKGROUND:Atopic dermatitis is a common inflammatory skin disorder that affects up to 15-20% of the population and is characterized by recurrent eczematous lesions with intense itching. As a heterogeneous disease, multiple factors have been suggested to explain the nature of atopic dermatitis (AD), and its high preval...
journal_title:Genes
pub_type: 杂志文章,评审
doi:10.3390/genes11040442
更新日期:2020-04-18 00:00:00
abstract::The liver plays a key role in metabolism and affects pig production. However, the functional annotation of noncoding regions of the pig liver remains poorly understood. We revealed the landscape of cis-regulatory elements and their functional characterization in pig liver. We identified 102,373 cis-regulatory elements...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes10050348
更新日期:2019-05-07 00:00:00
abstract::Changes of telomere length with age were assessed in diploid and triploid rainbow trout (Oncorhynchus mykiss) females in the cross-sectional study using Q-FISH technique. Triploid trout as sterile do not invest an energy in gametogenesis and continue to grow, whereas fertile diploid individuals suffer from declines in...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11070786
更新日期:2020-07-13 00:00:00
abstract::The increasing availability of large-scale time series data allows the inference of microbial community dynamics by association network analysis. However, correlation-based association network analyses are noninformative of causal, mediating and time-dependent relationships between microbial community functional facto...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes10030216
更新日期:2019-03-14 00:00:00