Abstract:
:In human pre-mRNA splicing, infrequent errors occur resulting in erroneous splice products as shown in a genome-wide approach. One characteristic subgroup consists of products lacking one cassette exon. The noise in the splicing process, represented by those misspliced products, can be increased by cold shock treatment or by inhibiting the nonsense mediated decay. Here, we investigated whether the splicing noise frequency increases with age in vivo in peripheral bloods cells or in vitro in cultured and aged fibroblasts from healthy donors. Splicing noise frequency was measured for four erroneously skipped NF1 exons and one exon of RABAC1, AATF and PCGF2 by RT-qPCR. Measurements were validated in cultured fibroblasts treated with cold shock or puromycin. Intragenic but not interpersonal differences were detected in splicing noise frequencies in vivo in peripheral blood cells of 11 healthy donors (15 y-85 y) and in in vitro senescent fibroblasts from three further donors. No correlation to the age of the donors was found in the splicing noise frequencies. Our data demonstrates that splicing error frequencies are not altered by age in peripheral blood cells or in vitro aged fibroblasts in the tested exons of the four investigated genes, indicating a high importance of correct splicing in these proliferating aged cells.
journal_name
Genes (Basel)journal_title
Genesauthors
Mellert K,Uhl M,Högel J,Lamla M,Kemkemer R,Kaufmann Ddoi
10.3390/genes2030562subject
Has Abstractpub_date
2011-08-02 00:00:00pages
562-77issue
3issn
2073-4425pii
genes2030562journal_volume
2pub_type
杂志文章相关文献
Genes文献大全abstract::Amazonian puffing snakes (Spilotes; Colubridae) are snakes widely distributed in the Neotropical region. However, chromosomal data are scarce in this group and, when available, are only limited to karyotype description using conventional staining. In this paper, we focused on the process of karyotype evolution and tre...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes10040288
更新日期:2019-04-09 00:00:00
abstract::The Sichuan partridge (Arborophila rufipectus, Phasianidae, Galliformes) is distributed in south-west China, and classified as endangered grade. To examine the evolution and genomic features of Sichuan partridge, we de novo assembled the Sichuan partridge reference genome. The final draft assembly consisted of approxi...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes10090677
更新日期:2019-09-05 00:00:00
abstract::Croatian viticulture was most extensive at the beginning of the 20th century, when about 400 varieties were in use. Autochthonous varieties are the result of spontaneous hybridization from the pre-phylloxera era and are still cultivated today on about 35 % of vineyard area, while some exist only in repositories. We pr...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11070737
更新日期:2020-07-02 00:00:00
abstract::Pleurotus tuoliensis (Pt) and P. eryngii var. eryngii (Pe) are important edible mushrooms. The epigenetic and gene expression signatures characterizing major developmental transitions in these two mushrooms remain largely unknown. Here, we report global analyses of DNA methylation and gene expression in both mushrooms...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes10060465
更新日期:2019-06-17 00:00:00
abstract::Hypersialylation caused by the overexpression of sialyltransferases (STs) is a common feature in cancer that is associated with several characteristics of tumorigenesis. Thus, identifying cancer-associated STs is critical for cancer therapy. However, ST screening has been frequently conducted in cell line models. In t...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11121436
更新日期:2020-11-28 00:00:00
abstract::The grizzly bear (Ursus arctos ssp. horribilis) represents the largest population of brown bears in North America. Its genome was sequenced using a microfluidic partitioning library construction technique, and these data were supplemented with sequencing from a nanopore-based long read platform. The final assembly was...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes9120598
更新日期:2018-11-30 00:00:00
abstract::Illumina and nanopore sequencing technologies are powerful tools that can be used to determine the bacterial composition of complex microbial communities. In this study, we compared nasal microbiota results at genus level using both Illumina and nanopore 16S rRNA gene sequencing. We also monitored the progression of n...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11091105
更新日期:2020-09-21 00:00:00
abstract::Acquisition of mobile elements by horizontal gene transfer can play a major role in bacterial adaptation and genome evolution by providing traits that contribute to bacterial fitness. However, gaining foreign DNA can also impose significant fitness costs to the host bacteria and can even produce detrimental effects. T...
journal_title:Genes
pub_type: 杂志文章,评审
doi:10.3390/genes11020142
更新日期:2020-01-29 00:00:00
abstract::Continuous cultures assure the invariability of environmental conditions and the metabolic state of cultured microorganisms, whereas batch-cultured cells undergo constant changes in nutrients availability. For that reason, continuous culture is sometimes employed in the whole transcriptome, whole proteome, or whole me...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11121419
更新日期:2020-11-27 00:00:00
abstract::The presence and strength of resource competition can influence how organisms adaptively respond to environmental change. Selection may thus reflect a balance between two forces, adaptation to an environmental optimum and evolution to avoid strong competition. While this phenomenon has previously been explored in the ...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11121433
更新日期:2020-11-28 00:00:00
abstract::Candida albicans mutants deficient in homologous recombination (HR) are extremely sensitive to the alkylating agent methyl-methane-sulfonate (MMS). Here, we have investigated the role of HR genes in the protection and repair of C. albicans chromosomes by taking advantage of the heat-labile property (55 °C) of MMS-indu...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes9090447
更新日期:2018-09-07 00:00:00
abstract::It is well established that embryonic chromosomal abnormalities (both in the number of chromosomes and the structure) account for 50% of early pregnancy losses. However, little is known regarding the potential differences in the incidence and distribution of chromosomal abnormalities between patients with sporadic abo...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes12020141
更新日期:2021-01-22 00:00:00
abstract::Hepatic oval cells (HOCs) are considered the progeny of the intrahepatic stem cells that are found in a small population in the liver after hepatocyte proliferation is inhibited. Due to their small number, isolation and capture of these cells constitute a challenging task for immunosensor technology. This work describ...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes9020089
更新日期:2018-02-14 00:00:00
abstract::The conserved Notch pathway is reported to be involved in progesterone synthesis and secretion; however, the exact effects remain controversial. To determine the role and potential mechanisms of the Notch signaling pathway in progesterone biosynthesis in porcine granulosa cells (pGCs), we first used a pharmacological ...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11020120
更新日期:2020-01-22 00:00:00
abstract::Perennial agriculture has been proposed as an option to improve the sustainability of cropping systems, by increasing the efficiency of resource use, while also providing ecosystem services. Neo-domestication, the contemporary domestication of plants that have not previously been used in agriculture, can be used to ge...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes9090422
更新日期:2018-08-21 00:00:00
abstract::We studied and compared the nucleolar expression or nucleoli from specific bivalents in spermatocytes of the standard Mus musculus domesticus 2n=40, of Robertsonian (Rb) homozygotes 2n = 24 and heterozygotes 2n = 32. We analyzed 200 nuclear microspreads of each specific nucleolar chromosome and spermatocyte karyotype,...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes10020120
更新日期:2019-02-06 00:00:00
abstract::Yersinia enterocolitica is the causative agent of yersiniosis, a zoonotic disease of growing epidemiological importance with significant consequences for public health. This pathogenic species has been intensively studied for many years. Six biotypes (1A, 1B, 2, 3, 4, 5) and more than 70 serotypes of Y. enterocolitica...
journal_title:Genes
pub_type: 杂志文章,评审
doi:10.3390/genes9050235
更新日期:2018-05-03 00:00:00
abstract::Although a number of methods have been proposed for identifying differentially expressed pathways (DEPs), few efforts consider the dynamic components of pathway networks, i.e., gene links. We here propose a signaling dynamics detection method for identification of DEPs, DynSig, which detects the molecular signaling ch...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes9070323
更新日期:2018-06-27 00:00:00
abstract::The Mexican population has one of the highest prevalences of metabolic syndrome (MetS) worldwide. The aim of this study was to investigate the association of single-nucleotide polymorphisms (SNPs) with MetS and its components. First, we performed a pilot Genome-wide association study (GWAS) scan on a sub-sample derive...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11101192
更新日期:2020-10-14 00:00:00
abstract::GOLDEN2-LIKE (GLK) is a member of the myeloblastosis (MYB) family transcription factor and it plays an important role in the regulation of plastid development and stress tolerance. In this study, a gene named AhGLK1b was identified from a cultivated peanut showing down-regulation in response to low calcium with a comp...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11030343
更新日期:2020-03-24 00:00:00
abstract::Engineered yeast are an important production platform for the biosynthesis of high-value compounds with medical applications. Recent years have witnessed several new developments in this area, largely spurred by advances in the field of synthetic biology and the elucidation of natural metabolic pathways. This minirevi...
journal_title:Genes
pub_type: 杂志文章,评审
doi:10.3390/genes9070340
更新日期:2018-07-06 00:00:00
abstract::The transcription factor PAX6 is essential in ocular development in vertebrates, being considered the master regulator of the eye. During eye development, it is essential for the correct patterning and formation of the multi-layered optic cup and it is involved in the developing lens and corneal epithelium. In adultho...
journal_title:Genes
pub_type: 杂志文章,评审
doi:10.3390/genes10121050
更新日期:2019-12-17 00:00:00
abstract::Hemp is a Pb-tolerant and Pb-accumulating plant and the study of its tolerance mechanisms could facilitate the breeding of hemp with enhanced Pb tolerance and accumulation. In the present study, we took advantage of sequential window acquisition of all theoretical mass spectra (SWATH) technology to study the differenc...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes10050396
更新日期:2019-05-22 00:00:00
abstract::The central goal of medical genomics is to understand the inherited basis of sequence variation that underlies human physiology, evolution, and disease. Functional association studies currently ignore millions of bases that span each centromeric region and acrocentric short arm. These regions are enriched in long arra...
journal_title:Genes
pub_type: 杂志文章,评审
doi:10.3390/genes10050352
更新日期:2019-05-08 00:00:00
abstract::Genomic imprinting in domestic animals contributes to the variance of performance traits. However, research remains to be done on large-scale detection of epigenetic landscape of porcine imprinted loci including the GNAS complex locus. The purpose of this study was to generate porcine parthenogenetic fetuses and compr...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes11010096
更新日期:2020-01-14 00:00:00
abstract::This article explores the mechanism of miR-194 on the proliferation and apoptosis of Aβ1-42-transduced hippocampal neurons. Aβ1-42-transduced hippocampal neuron model was established by inducing hippocampal neurons with Aβ1-42. MTT assay and flow cytometry were used to detect the viability and apoptosis of hippocampal...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes10040313
更新日期:2019-04-21 00:00:00
abstract::The liver plays a key role in metabolism and affects pig production. However, the functional annotation of noncoding regions of the pig liver remains poorly understood. We revealed the landscape of cis-regulatory elements and their functional characterization in pig liver. We identified 102,373 cis-regulatory elements...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes10050348
更新日期:2019-05-07 00:00:00
abstract::Ehlers-Danlos syndrome (EDS) comprises clinically heterogeneous connective tissue disorders with diverse molecular etiologies. The 2017 International Classification for EDS recognized 13 distinct subtypes caused by pathogenic variants in 19 genes mainly encoding fibrillar collagens and collagen-modifying or processing...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes10020135
更新日期:2019-02-12 00:00:00
abstract::Trees and their seeds regulate their germination, growth, and reproduction in response to environmental stimuli. These stimuli, through signal transduction, trigger transcription factors that alter the expression of various genes leading to the unfolding of the genetic program. A regulon is conceptually defined as a s...
journal_title:Genes
pub_type: 杂志文章,评审
doi:10.3390/genes11090995
更新日期:2020-08-25 00:00:00
abstract::Long non-coding (lnc) RNAs serve a multitude of functions in cells. NEAT1 RNA is a highly abundant 4 kb lncRNA in nuclei, and coincides with paraspeckles, nuclear domains that control sequestration of paraspeckle proteins. We examined NEAT1 RNA levels and its function in 3T3-L1 cells during differentiation to adipocyt...
journal_title:Genes
pub_type: 杂志文章
doi:10.3390/genes5041050
更新日期:2014-11-27 00:00:00