Abstract：
:Hepatic oval cells (HOCs) are considered the progeny of the intrahepatic stem cells that are found in a small population in the liver after hepatocyte proliferation is inhibited. Due to their small number, isolation and capture of these cells constitute a challenging task for immunosensor technology. This work describes the development of a 3D-printed continuous flow system and exploits disposable screen-printed electrodes for the rapid detection of HOCs that over-express the OV6 marker on their membrane. Multiwall carbon nanotube (MWCNT) electrodes have a chitosan film that serves as a scaffold for the immobilization of oval cell marker antibodies (anti-OV6-Ab), which enhance the sensitivity of the biomarker and makes the designed sensor specific for oval cells. The developed sensor can be easily embedded into the 3D-printed flow cell to allow cells to be exposed continuously to the functionalized surface. The continuous flow is intended to increase capture of most of the target cells in the specimen. Contact angle measurements were performed to characterize the nature and quality of the modified sensor surface, and electrochemical measurements (cyclic voltammetry (CV) and square wave voltammetry (SWV)) were performed to confirm the efficiency and selectivity of the fabricated sensor to detect HOCs. The proposed method is valuable for capturing rare cells and could provide an effective tool for cancer diagnosis and detection.
journal_name
Genes (Basel)journal_title
Genesauthors
Damiati S,Peacock M,Leonhardt S,Damiati L,Baghdadi MA,Becker H,Kodzius R,Schuster Bdoi
10.3390/genes9020089subject
Has Abstractpub_date
2018-02-14 00:00:00issue
2issn
2073-4425pii
genes9020089journal_volume
9pub_type
杂志文章相关文献
Genes文献大全abstract：:DNA methylation plays several roles in regulating neuronal proliferation, differentiation, and physiological functions. The major de novo methyltransferase, DNMT3, controls the DNA methylation pattern in neurons according to environmental stimulations and behavioral regulations. Previous studies demonstrated that knoc...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11111322
更新日期：2020-11-07 00:00:00
abstract：:The recently discovered Methanonatronarchaeia are extremely halophilic and moderately thermophilic methyl-reducing methanogens representing a novel class-level lineage in the phylum Euryarchaeota related to the class Halobacteria. Here we present a detailed analysis of 1D-nano liquid chromatography-electrospray ioniza...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes9020028
更新日期：2018-01-23 00:00:00
abstract：:Neurofibromatosis type 1 (NF1) is one of the most common autosomal dominant genetic diseases. It is caused by mutations in the NF1 gene encoding for the large protein, neurofibromin. Genetic testing of NF1 is cumbersome because 50% of cases are sporadic, and there are no mutation hot spots. In addition, the most recog...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes9040216
更新日期：2018-04-17 00:00:00
abstract：:Increasing evidence suggests that dysregulation of microRNAs (miRNAs) may lead to a variety of diseases. Therefore, identifying disease-related miRNAs is a crucial problem. Currently, many computational approaches have been proposed to predict binary miRNA-disease associations. In this study, in order to predict under...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes9030139
更新日期：2018-03-02 00:00:00
abstract：:Mitogen-activated protein kinase (MAPK) signaling pathway plays key roles in sensing extracellular signals and transmitting them from the cell membrane to the nucleus in response to various environmental stimuli. A MAPKKK protein CgMck1 in Colletotrichum gloeosporioides was characterized. Phenotypic analyses of the ∆C...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes9110543
更新日期：2018-11-08 00:00:00
abstract：:The prevalence of the so-called diseases of affluence, such as type 2 diabetes or hypertension, has increased dramatically in the last two generations. Although genome-wide association studies (GWAS) have discovered hundreds of genes involved in disease etiology, the sudden increase in disease incidence suggests a maj...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes9080411
更新日期：2018-08-14 00:00:00
abstract：:Xeroderma pigmentosum group C (XPC) is a key component of the nucleotide excision repair (NER) pathway. Dysfunctional XPC protein may impair NER-mediated DNA repair capacity and further lead to genomic instability and carcinogenesis. Two common nonsynonymous polymorphisms in the XPC gene, Lys939Gln (rs2228001 A > C) a...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes7100073
更新日期：2016-09-24 00:00:00
abstract：:Bupleurum falcatum, which belongs to the family Apiaceae, has long been applied for curative treatments, especially as a liver tonic, in herbal medicine. The chloroplast (cp) genome has been an ideal model to perform the evolutionary and comparative studies because of its highly conserved features and simple structure...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes7050020
更新日期：2016-05-13 00:00:00
abstract：:Genetic dissection of yield components and seed mineral-nutrient is crucial for understanding plant physiological and biochemical processes and alleviate nutrient malnutrition. Sesame (Sesamum indicum L.) is an orphan crop that harbors rich allelic repertoire for seed mineral-nutrients. Here, we harness this wide dive...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11101221
更新日期：2020-10-18 00:00:00
abstract：:Transfer RNAs (tRNAs) are abundant small non-coding RNAs that are crucially important for decoding genetic information. Besides fulfilling canonical roles as adaptor molecules during protein synthesis, tRNAs are also the source of a heterogeneous class of small RNAs, tRNA-derived small RNAs (tsRNAs). Occurrence and th...
journal_title：Genes
pub_type： 杂志文章,评审
doi：10.3390/genes9120607
更新日期：2018-12-05 00:00:00
abstract：:Serotonin receptor 2A (HTR2A) is an important signalling factor implicated in cognitive functions and known to be associated with schizophrenia. The biological significance of HTR2A in schizophrenia remains unclear as molecular analyses including genetic association, mRNA expression and methylation studies have report...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes8010014
更新日期：2017-01-04 00:00:00
abstract：:Feed cost accounts for approximately 65-75% of overall commercial pork production costs. Therefore, improving the feed efficiency of pig production is important. In this study, 12 individuals with either extremely high (HE) or low (LE) feed efficiency were selected from 225 Duroc × (Landrace × Yorkshire) (DLY) pigs. A...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes10090725
更新日期：2019-09-18 00:00:00
abstract：:In human pre-mRNA splicing, infrequent errors occur resulting in erroneous splice products as shown in a genome-wide approach. One characteristic subgroup consists of products lacking one cassette exon. The noise in the splicing process, represented by those misspliced products, can be increased by cold shock treatmen...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes2030562
更新日期：2011-08-02 00:00:00
abstract：:In addition to its wide geographical distribution, osteoglossiform fishes represent one of the most ancient freshwater teleost lineages; making it an important group for systematic and evolutionary studies. These fishes had a Gondwanan origin and their past distribution may have contributed to the diversity present in...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes9060306
更新日期：2018-06-19 00:00:00
abstract：:This review discusses a set of experimental results that support the existence of extended strand displacement events during budding yeast lagging strand DNA synthesis. Starting from introducing the mechanisms and factors involved in leading and lagging strand DNA synthesis and some aspects of the architecture of the ...
journal_title：Genes
pub_type： 杂志文章,评审
doi：10.3390/genes10020167
更新日期：2019-02-21 00:00:00
abstract：:Hepatitis B virus (HBV) is divided into nine genotypes, A to I. Currently, it remains unclear how the individual genotypes contribute to the estimated 250 million chronic HBV infections. We performed a literature search on HBV genotyping data throughout the world. Over 900 publications were assessed and data were extr...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes9100495
更新日期：2018-10-15 00:00:00
abstract：:Variants of the LOXHD1 gene, which are expressed in hair cells of the cochlea and vestibule, have been reported to cause a progressive form of autosomal recessive non-syndromic hereditary hearing loss, DFNB77. In this study, genetic screening was conducted on 8074 Japanese hearing loss patients utilizing massively par...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes10100735
更新日期：2019-09-23 00:00:00
abstract：:We analysed nine microsatellite markers for 626 individuals representing the geographic range of eight closely related endemic New Zealand species of Sophora. Structure analysis identified the optimal K value as seven, with samples identified as Sophorachathamica, Sophorafulvida, Sophoralongicarinata, and Sophoraprost...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes9020111
更新日期：2018-02-20 00:00:00
abstract：:Acute myeloid leukemia (AML) can develop as a secondary malignancy following radiotherapy, but also following low-dose environmental or occupational radiation exposure. Therapy-related AML frequently carries deletions of chromosome 5q and/or 7, but for low-dose exposure associated AML this has not been described. For ...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes2020384
更新日期：2011-05-31 00:00:00
abstract：:In genome analysis, k-mer-based comparison methods have become standard tools. However, even though they are able to deliver reliable results, other algorithms seem to work better in some cases. To improve k-mer-based DNA sequence analysis and comparison, we successfully checked whether adding positional resolution is...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes8040122
更新日期：2017-04-19 00:00:00
abstract：:Recent advances in next-generation sequencing technology have led to the production of an unprecedented volume of genomic data, thus further advancing our understanding of the role of genetic variation in clinical pharmacogenomics. In the present study, we used whole exome sequencing data from 50,726 participants, as ...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11050561
更新日期：2020-05-18 00:00:00
abstract：:Circadian clocks in eukaryotes involve both transcriptional-translational feedback loops, post-translational regulation, and metabolic, non-transcriptional oscillations. We recently identified the involvement of circadian oscillations in the intracellular concentrations of magnesium ions (Mg2+i) that were conserved in...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes10020158
更新日期：2019-02-19 00:00:00
abstract：:Pancreatic ductal adenocarcinoma is an extremely aggressive disease with a high metastatic potential. Most patients are diagnosed with metastatic disease, at which the five-year survival rate is only 3%. A better understanding of the mechanisms that drive metastasis is imperative for the development of better therapeu...
journal_title：Genes
pub_type： 杂志文章,评审
doi：10.3390/genes11010006
更新日期：2019-12-19 00:00:00
abstract：:The study of DNA to predict externally visible characteristics (EVCs) and the biogeographical ancestry (BGA) from unknown samples is gaining relevance in forensic genetics. Technical developments in Massively Parallel Sequencing (MPS) enable the simultaneous analysis of hundreds of DNA markers, which improves successf...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11060708
更新日期：2020-06-26 00:00:00
abstract：:Limb-girdle muscular dystrophies (LGMD) are clinically and genetically heterogeneous conditions, presenting with a wide clinical spectrum, leading to progressive proximal weakness caused by loss of muscle fibers. MiR-206 is a member of myomiRNAs, a group of miRNAs with important function in skeletal muscle. Our aim is...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes12010085
更新日期：2021-01-12 00:00:00
abstract：:The homeodomain-leucine zipper (HD-Zip) gene family, as plant-specific transcription factors, plays an important role in plant development and growth as well as in the response to diverse stresses. Although HD-Zip genes have been extensively studied in many plants, they had not yet been studied in wheat, especially th...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes9020070
更新日期：2018-02-01 00:00:00
abstract：:Cyclocarya paliurus (Batal.) Iljinskaja, a unique species growing in southern China, is a multi-function tree species with medicinal, healthcare, material, and ornamental values. So far, sexual reproduction is the main method for extensive cultivation of C. paliurus plantations, but this is limited by low seed plumpne...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes10100818
更新日期：2019-10-17 00:00:00
abstract：:Genetic adaptation to extremes is a fascinating topic. Nevertheless, few studies have explored the genetic adaptation of closely related species respectively inhabiting distinct extremes. With deep transcriptome sequencing, we attempt to detect the genetic architectures of tadpoles of five closely related toad species...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11020123
更新日期：2020-01-22 00:00:00
abstract：:Propionibacteria have been studied extensively since the early 1930s due to their relevance to industry and importance as human pathogens. Still, their unique metabolism is far from fully understood. This is partly due to their signature high GC content, which has previously hampered the acquisition of quality sequenc...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11101115
更新日期：2020-09-23 00:00:00
abstract：:Autoimmune blistering dermatoses (ABDs) are characterized by autoantibodies to keratinocyte surface antigens and molecules within the dermal-epidermal junction causing disruption of skin integrity. The affinity of Fc receptors (FcRs) causing an autoimmune response in ABDs may vary based on single-nucleotide polymorphi...
journal_title：Genes
pub_type： 杂志文章
doi：10.3390/genes11040371
更新日期：2020-03-30 00:00:00
