Abstract:
:Using freshly isolated single smooth muscle cells prepared by collagenase treatment, membrane currents were recorded by whole-cell voltage clamp. Intracellular constituents were modified by using an intracellular perfusion technique, i.e., pipette solutions were continuously exchanged from control to test solutions during current recording. In smooth muscle cells, intracellular application of ATP, but not cyclic AMP, enhanced the amplitude of Ca2+ currents and prevented current run-down. In addition, with this stabilization of Ca2+ current recording by ATP, introduction of various chemicals into the cell using the intracellular perfusion technique is useful for investigations of regulation of ion channels in smooth muscle cells.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Ohya Y,Sperelakis Ndoi
10.1007/BF00231006subject
Has Abstractpub_date
1988-03-01 00:00:00pages
79-86issue
1-2eissn
0300-8177issn
1573-4919journal_volume
80pub_type
杂志文章abstract::Administration of Ca2+ channel blockers in cardiac disorders and the central role of Ca2+ in modulating neutrophil functions, prompted us to investigate whether administration of nifedipine to mice would alter their natural resistance to infectious agents like Salmonella typhimurium. Neutrophil chemiluminescence (CL) ...
journal_title:Molecular and cellular biochemistry
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
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更新日期:2013-01-01 00:00:00
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更新日期:2005-08-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
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journal_title:Molecular and cellular biochemistry
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
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更新日期:2016-06-01 00:00:00
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journal_title:Molecular and cellular biochemistry
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
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更新日期:2004-08-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
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更新日期:2002-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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journal_title:Molecular and cellular biochemistry
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更新日期:2001-02-01 00:00:00
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