Abstract:
OBJECTIVES:Mortality associated with carbapenemase-producing Enterobacteriaceae is high as there are few therapeutic options. Escherichia coli sequence type 410 (ST410) is currently an international high-risk clone and is responsible for a large number of clinical infections. Here we report the draft genome sequence of a ST410 clinical E. coli isolate (ECS9) co-harbouring blaNDM-5, blaOXA-1, blaCTX-M-15, blaCMY-2, aac(3)-IIa and aac(6')-Ib-cr genes, obtained from a patient with bloodstream infection in China. METHODS:The genome of E. coli ECS9 was sequenced using an Illumina HiSeqTM 4000 instrument with a 150-bp paired-end approach. Generated sequence reads were assembled using Velvet 1.2.10. Contigs were annotated using Rapid Annotation using Subsystem Technology (RAST), and further whole-genome sequence data analyses were performed. RESULTS:Escherichia coli ECS9 belongs to multilocus sequence typing (MLST) ST410. The total number of assembled bases was 4 935 145 bp, with 5077 protein-coding sequences. The presence of the blaNDM-5, blaOXA-1, blaCTX-M-15 and blaCMY-2 genes was detected in addition to other antimicrobial resistance genes conferring resistance to fluoroquinolones, aminoglycosides, trimethoprim, sulfonamides and tetracyclines. CONCLUSION:To our knowledge, this is the first report of anE. coli ST410 strain co-harbouring blaNDM-5, blaOXA-1, blaCTX-M-15, blaCMY-2, aac(3)-IIa and aac(6')-Ib-cr, obtained from a bloodstream infection in China. The presented genome sequence of carbapenemase-producing E. coli strain ST410 could provide further insight into the acquisition of multiple resistance genes by this successful lineage.
journal_name
J Glob Antimicrob Resistjournal_title
Journal of global antimicrobial resistanceauthors
Huang J,Ma S,Yu Q,Fu M,Shao L,Shan X,Li Xdoi
10.1016/j.jgar.2019.10.027subject
Has Abstractpub_date
2019-12-01 00:00:00pages
354-355eissn
2213-7165issn
2213-7173pii
S2213-7165(19)30289-9journal_volume
19pub_type
杂志文章abstract:OBJECTIVES:Staphylococcus aureus remains the most important cause of infections in hospitals and long-term care facilities. The aim of this study was to analyse the resistance, virulence, and epidemiological and genetic relationships of S. aureus from bloodstream infections (BSIs) and pneumonia from patients in Souther...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.07.009
更新日期:2017-12-01 00:00:00
abstract:OBJECTIVES:Corynebacterium urealyticum is a non-diphtherial urease-producing clinically relevant corynebacterium associated with urinary tract infections. Most clinical C. urealyticum isolates are multidrug-resistant. Whole-genome sequencing (WGS) of C. urealyticum VH4248 isolated from a clinical urine sample at Hospit...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.07.020
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVES:Here we report the finding of three copies of the blaKPC-2 gene on a plasmid in ST11 Klebsiella pneumoniae. METHODS:Carbapenem-resistant K. pneumoniae clinical strain WCHKP2 was subjected to whole-genome sequencing (WGS) using both a short-read Illumina HiSeq X10 platform and long-read MinION sequencer. Hyb...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2018.11.017
更新日期:2019-06-01 00:00:00
abstract:OBJECTIVES:We analysed the concordance of rectal swab isolates and blood culture for Gram-negative bacteria (GNB) isolates in neonates with a suspicion of neonatal sepsis admitted to a neonatal care unit in Haiti. METHODS:We matched pairs of blood and rectal samples taken on the date of suspected sepsis onset in the s...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.08.017
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVES:Infections caused by multidrug-resistant Gram-negative bacteria are associated with high mortality. A relevant concern is the efficacy of antibiotic therapy in burn patients in whom pathophysiological changes strongly influence pharmacokinetic (PK) parameters. This study aimed to describe the PK parameters o...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.06.011
更新日期:2020-03-01 00:00:00
abstract::Antibiotic resistance genes are rapidly spread between pathogens and the normal flora, with plasmids playing an important role in their circulation. This study aimed to investigate antibiotic resistance plasmids in the gut microbiome of hospitalised patients. Stool samples were collected from seven inpatients at Sirir...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2016.03.001
更新日期:2016-09-01 00:00:00
abstract:OBJECTIVE:Little is known about the treatment outcomes of HIV-negative drug-resistant tuberculosis (TB) patients in Malaysia. With respect to this issue, this study aimed to determine factors associated with unsuccessful treatment outcomes among drug-resistant TB patients at the Institute of Respiratory Medicine, Kuala...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.05.009
更新日期:2019-12-01 00:00:00
abstract::Enterococcus faecium was considered an important nosocomial pathogen that could incur a substantial health burden owing to its high drug resistance and its ability to adapt to the hospital niche. Here we presented the draft genome sequence of multidrug-resistant E. faecium XH877. The draft genome sequence was composed...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2016.07.004
更新日期:2016-12-01 00:00:00
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journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2016.07.012
更新日期:2016-12-01 00:00:00
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journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.09.008
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abstract:OBJECTIVES:The aim of this study was to determine the prevalence of nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA) among healthcare workers (HCWs) compared with non-HCWs at Duhok city, Kurdistan Region, northern Iraq. METHODS:A total of 182 HCWs with different occupations and working in different...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.01.010
更新日期:2017-06-01 00:00:00
abstract::Uropathogenic Escherichia coli (UPEC) is the most frequent cause of urinary tract infection (UTI). Virulence factors (VFs) of UPEC in children are not well known. Circulating antibiotic resistance mechanisms in the community are increasing. In this study, the aetiological agents of UTI and antibiotic resistance mechan...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2014.08.003
更新日期:2014-12-01 00:00:00
abstract::Staphylococcus pseudintermedius is a frequent pathogen in dogs. The emergence of meticillin-resistant S. pseudintermedius (MRSP), which is concomitantly resistant to nearly all veterinary licensed antibiotics used for systemic treatment in dogs, is a major problem for veterinarians. In France, 16.9% (41/243) of the S....
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2014.02.002
更新日期:2014-06-01 00:00:00
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journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.03.011
更新日期:2020-09-01 00:00:00
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journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.12.018
更新日期:2020-06-01 00:00:00
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journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.02.020
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVES:This study aimed to characterise insertional mutations disturbing themgrB gene in carbapenem-resistant Klebsiella pneumoniae (CRKp). METHODS:A total of 118 clinical CRKp isolates were surveyed for polymyxin resistance and insertion sequence (IS) elements disruptingmgrB. RESULTS:Of the 118 isolates, 78 (66....
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.11.003
更新日期:2020-11-24 00:00:00
abstract::Acinetobacter baumannii coccobacilli are dangerous to patients in intensive care units because of their multidrug resistance to antibiotics, developed mainly in the past decade. This study aimed to examine whether there is a significant correlation between the number of Pro-Ala repeats in the CAP01997 protein, the Emr...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2016.09.004
更新日期:2016-12-01 00:00:00
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journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.09.017
更新日期:2018-03-01 00:00:00
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journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.04.031
更新日期:2020-09-01 00:00:00
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journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.06.019
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVES:Recently, a growing resistance to antimalarial drugs such as chloroquine, sulfadoxine-pyrimethamine, artemisinin derivatives and mefloquine has been observed. The pharmacokinetic limitation of the current therapy and multi-drug resistance has resulted in an urgent need to study the new antimalarial combinati...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.02.018
更新日期:2020-09-01 00:00:00
abstract::According to the World Health Organization (WHO), as of today, there are 2.165.500 confirmed cases of the novel coronavirus disease (COVID-19) and 145.705 deaths in over 185 countries. Unfortunately, despite the tremendous efforts to develop a vaccine initiated by various leading health institutions all over the world...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.07.005
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to determine the antimicrobial susceptibility of Neisseria gonorrhoeae isolates in the Lao People's Democratic Republic (Laos). METHODS:A total of 165 gonococcal isolates (1.3%) were obtained from 12 281 genital samples routinely submitted to a diagnostic laboratory in Vientiane, L...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.12.001
更新日期:2018-06-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to determine the percentage of antimicrobial-resistant isolates and the associated resistance mechanisms in Gram-negative bacteria from South Western Nigeria. METHODS:A total of 306 non-duplicate unbiased Gram-negative isolates were recovered from patients admitted to three teachin...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.10.016
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVES:The rapid spread of multidrug-resistant (MDR) Acinetobacter baumannii poses a substantial threat for morbidity and mortality worldwide. In particular, carbapenem-resistant A. baumannii has caused a severe challenge to human health. Here we reported the draft genome sequence of A. baumannii S131434, an OXA-23...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2018.11.008
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to validate the use of LML antimicrobial gradient strips for quantitative determination of carbapenem susceptibility in Enterobacteriaceae. METHODS:A total of 95 non-redundant Enterobacteriaceae strains isolated during 2012-2014 were used for this validation study. Initially, LML a...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2016.10.010
更新日期:2017-03-01 00:00:00
abstract:OBJECTIVES:This study aimed to isolate and characterise extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E) isolates from animals and wastewater in Tunisia. METHODS:ESBL-E from wastewater (n=123 samples), faeces of healthy animals (poultry, sheep, goats and calves) (n=140) and raw milk from healthy cow...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.01.002
更新日期:2019-06-01 00:00:00
abstract:OBJECTIVES:Multidrug-resistant (MDR) enterococci are found extensively in food samples. This study characterized the phenotypic virulence factors and the ability of horizontal gene transfer of a streptomycin resistance gene among enterococci isolated from fermented pork. METHODS:Thirty-six MDR enterococci were subject...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.04.016
更新日期:2020-09-01 00:00:00
abstract::In this study, 26 clinical isolates of Mycobacterium abscessus complex strains were characterised using available identification algorithms for the three species (M. abscessus, M. massiliense and M. bolletii) and the genotypic characteristics of clarithromycin (CLR) resistance were determined. Strains were identified ...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2015.05.007
更新日期:2015-09-01 00:00:00