High prevalence of blaCMY AmpC beta-lactamase in ESBL co-producing Escherichia coli and Klebsiella spp. clinical isolates in the northeast of Iran.

Abstract:

OBJECTIVE:The production of β-lactamase enzymes such as AmpC β-lactamases and extended-spectrum β-lactamases (ESBLs) is among the main mechanisms for resistance to expanded-spectrum cephalosporins. The present study was conducted to investigate the prevalence and molecular epidemiology of plasmid-mediated AmpC beta (β)-lactamase in ESBL co-producing Escherichia coli (E. coli) and Klebsiella spp. (Klebsiella pneumoniae and Klebsiella oxytoca) clinical isolates in the northeast of Iran. METHODS:A total of 602 E. coli and Klebsiella spp. clinical isolates were collected from three hospitals in Mashhad (northeast of Iran). A combination disk test (CDT) was performed for the phenotypic detection of ESBLs. Screening for the detection of AmpC β-lactamases was performed by a susceptibility test to a cefoxitin disc among ESBL producing isolates. A confirmatory test for AmpC β-lactamases was performed using the Mast® D68C test. Identification of plasmid-mediated AmpC cluster genes was done by multiplex polymerase chain reaction (PCR). RESULTS:Among 336 ESBL-producing strains, 230 (68.4%) isolates were resistant to cefoxitin. Results of the Mast® D68C test showed that 30% (69/230) of cefoxitin-resistant isolates simultaneously exhibited ESBL and AmpC activity and 22% (51/230) of isolates probably showed multi-drug resistant (MDR) phenotype. Results of multiplex PCR among ESBL-positive isolates showed that, 16.7% (56/336) of isolates were positive for plasmid-borneampC cluster genes, and CMY (38%) was the most frequent genotype of plasmid mediated AmpC. CONCLUSION:Findings of the study revealed that an increase in the prevalence of ESBL and AmpC co-producer in E. coli and Klebsiella spp. strains may become an important public health issue. Therefore, there is a vital need for surveillance of spread of these clinical isolates.

authors

Rizi KS,Mosavat A,Youssefi M,Jamehdar SA,Ghazvini K,Safdari H,Amini Y,Farsiani H

doi

10.1016/j.jgar.2020.03.011

subject

Has Abstract

pub_date

2020-09-01 00:00:00

pages

477-482

eissn

2213-7165

issn

2213-7173

pii

S2213-7165(20)30075-8

journal_volume

22

pub_type

杂志文章
  • Meropenem pharmacokinetics during extracorporeal membrane oxygenation and continuous haemodialysis: a case report.

    abstract:OBJECTIVES:Pharmacokinetic (PK) parameters can change significantly during extracorporeal membrane oxygenation (ECMO) and continuous haemodialysis. This case report describes the pharmacokinetics of a 3-h meropenem infusion in an infantile anuric patient on ECMO with continuous haemodialysis. CASE:A 19-month-old femal...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2020.04.029

    authors: Saito J,Shoji K,Oho Y,Aoki S,Matsumoto S,Yoshida M,Nakamura H,Kaneko Y,Hayashi T,Yamatani A,Capparelli E,Miyairi I

    更新日期:2020-09-01 00:00:00

  • New chalcone compound as a promising antileishmanial drug for an old neglected disease: Biological evaluation using radiolabelled biodistribution.

    abstract:OBJECTIVES:Treatment of leishmaniasis remains a challenge, especially due to the need for multiple painful injections, the toxicity of current drugs against the disease, their lack of efficacy and, lately, drug resistance. The aim of this study was to demonstrate the biological behaviour of 3-nitro-2'-hydroxy-4',6'-dim...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2017.11.012

    authors: Sousa-Batista AJ,Philipon CIMS,de Souza Albernaz M,Pinto SR,Rossi-Bergmann B,Santos-Oliveira R

    更新日期:2018-06-01 00:00:00

  • High burden of antimicrobial drug resistance in Asia.

    abstract::The rapid development of antimicrobial resistance among micro-organisms is a serious public health concern. Moreover, the dissemination of antibiotic-resistant bacteria makes this issue a global problem, and Asia is no exception. For example, since New Delhi metallo-β-lactamase (NDM)-producing Enterobacteriaceae were ...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章,评审

    doi:10.1016/j.jgar.2014.02.007

    authors: Lai CC,Lee K,Xiao Y,Ahmad N,Veeraraghavan B,Thamlikitkul V,Tambyah PA,Nelwan RH,Shibl AM,Wu JJ,Seto WH,Hsueh PR

    更新日期:2014-09-01 00:00:00

  • Antimicrobial susceptibility in hospitals in Hong Kong: The current status 2009-2011.

    abstract::Hospitals in Hong Kong, like many hospitals in the world, are constantly challenged by the increasing rate of non-susceptible and multidrug-resistant organisms (MDROs). Accurate and timely surveillance is essential for effective control. The Hospital Authority of Hong Kong has developed a comprehensive antimicrobial s...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章,评审

    doi:10.1016/j.jgar.2014.05.008

    authors: Lai CK,Chuang WM,Kong MY,Siu HK,Tsang DN

    更新日期:2014-12-01 00:00:00

  • Draft genome sequence of a multidrug-resistant KPC-2-producing Enterobacter aerogenes isolated from a hospitalised patient in Brazil.

    abstract:OBJECTIVES:Multidrug-resistant (MDR) Enterobacter aerogenes strains are frequently associated with nosocomial infections and high mortality rates, representing a serious public health problem. The aim of this study was to present the draft genome sequence of a MDR KPC-2-producing E. aerogenes isolated from a perineal s...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2017.07.016

    authors: Moura Q,Fernandes MR,Cerdeira L,Nhambe LF,Ienne S,Souza TA,Lincopan N

    更新日期:2017-09-01 00:00:00

  • A systematic review on the prevalence of extended-spectrum beta lactamase-producing Gram-negative bacteria in Nigeria.

    abstract:OBJECTIVE:There are numerous reported cases of extended-spectrum beta-lactamases (ESBLs) producing Enterobacteriaceae in Nigeria. Yet, there are regions in Nigeria where cases of ESBL were under-reported. The review aims to assess the prevalence of ESBL-producing Gram-negative bacteria in Nigeria and the genes involved...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章,评审

    doi:10.1016/j.jgar.2020.04.010

    authors: Tanko N,Bolaji RO,Olayinka AT,Olayinka BO

    更新日期:2020-09-01 00:00:00

  • Resistance to antimalarial drugs: An endless world war against Plasmodium that we risk losing.

    abstract::The objective of this review was to describe the 'state of the art' of Plasmodium falciparum resistance to the main antimalarial drugs. A brief note on Plasmodium vivax is also included. Resistance of P. falciparum to the various antimalarials has a long history of hits and misses. During the last 60 years, the pace a...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章,评审

    doi:10.1016/j.jgar.2015.02.002

    authors: Severini C,Menegon M

    更新日期:2015-06-01 00:00:00

  • Risk factors for carbapenem-resistant Klebsiella pneumoniae infection: A meta-analysis.

    abstract:OBJECTIVES:Rates of nosocomial infections caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) have increased. A meta-analysis was conducted to explore risk factors for CRKP infection in order to provide a theoretical basis for reducing the CRKP infection rate and actively preventing CRKP infection. METHODS:Onl...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2019.09.006

    authors: Li J,Li Y,Song N,Chen Y

    更新日期:2020-06-01 00:00:00

  • Detection of chromosomal and plasmid-mediated mechanisms of colistin resistance in Escherichia coli and Klebsiella pneumoniae from Indian food samples.

    abstract:OBJECTIVES:Numerous previous publications on the detection of bacterial isolates harbouring the mcr-1 gene from animals and humans strongly suggest an underlying route of transmission of colistin resistance via the food chain. The aim of this study was to investigate the presence of colistin-resistant (Col-R) bacteria ...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2018.09.005

    authors: Ghafur A,Shankar C,GnanaSoundari P,Venkatesan M,Mani D,Thirunarayanan MA,Veeraraghavan B

    更新日期:2019-03-01 00:00:00

  • Emergence in Japan of an isolate of Klebsiella pneumoniae co-harbouring blaKPC-2 and rmtB.

    abstract:OBJECTIVES:Here we describe a clinical isolate of Klebsiella pneumoniae ST11 harbouring both blaKPC-2 and rmtB genes in Japan. METHODS:A carbapenem- and aminoglycoside-resistant K. pneumoniae was isolated from an inpatient in Japan. Whole-genome sequencing (WGS) was performed using an Illumina next-generation sequence...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2018.11.026

    authors: Uchida H,Tada T,Tohya M,Sugahara Y,Kato A,Miyairi I,Kirikae T

    更新日期:2019-06-01 00:00:00

  • Trends and factors associated with antimicrobial resistance of Acinetobacter spp. invasive isolates in Europe: A country-level analysis.

    abstract:OBJECTIVES:This study investigated trends and factors associated with antimicrobial resistance (AMR) in Acinetobacter spp. in Europe. METHODS:Using data from EARS-Net, population-weighted multilevel logistic regression models with random intercepts for each participating country were performed to assess trends in Acin...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2018.05.024

    authors: Alvarez-Uria G,Midde M

    更新日期:2018-09-01 00:00:00

  • Carbapenem-resistant Klebsiella pneumoniae infections in a Greek intensive care unit: Molecular characterisation and treatment challenges.

    abstract::Acquisition of carbapenemase-producing Klebsiella pneumoniae (CP-Kp) strains poses a major threat to critically ill patients. The objectives of this study were to describe the epidemiology of CP-Kp isolates as well as the clinical outcome associated with the corresponding infections and to identify risk factors for mo...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2015.01.006

    authors: Katsiari M,Panagiota G,Likousi S,Roussou Z,Polemis M,Alkiviadis Vatopoulos C,Evangelia Platsouka D,Maguina A

    更新日期:2015-06-01 00:00:00

  • Comparative study of the impact of the administration of Amoxicillin and Algo-Bio® alternative substance to antibiotics, on the level of selection of resistant Enterobacteriaceae in the digestive flora of piglets.

    abstract:OBJECTIVES:The aim of study was to evaluate by comparative study the level of selection of antibiotic-resistant Enterobacteriaceae in the digestive microbiota of piglets when using amoxicillin and Algo-Bio®. METHODS:Amoxicillin and Algo-Bio® administration was carried out over a period of 5 days (D0-D4) at a dose of 1...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2018.01.008

    authors: Kouadio IK,Guessennd N,Dadié A,Koffi E,Dosso M

    更新日期:2018-06-01 00:00:00

  • Global antibacterial resistance: The never-ending story.

    abstract::Bacterial resistance is undoubtedly recognised as a major medical challenge in most healthcare systems. Resistance-determining genes, mostly in combination, and multidrug-resistant (MDR) pathogens are spreading with unprecedented speed. Well known resistance carriers with high clinical impact include the Gram-positive...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章,评审

    doi:10.1016/j.jgar.2013.03.010

    authors: Theuretzbacher U

    更新日期:2013-06-01 00:00:00

  • Antimicrobial susceptibility of Neisseria gonorrhoeae isolates in Vientiane, Lao PDR.

    abstract:OBJECTIVES:The aim of this study was to determine the antimicrobial susceptibility of Neisseria gonorrhoeae isolates in the Lao People's Democratic Republic (Laos). METHODS:A total of 165 gonococcal isolates (1.3%) were obtained from 12 281 genital samples routinely submitted to a diagnostic laboratory in Vientiane, L...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2017.12.001

    authors: Phouangsouvanh S,Mayxay M,Keoluangkhot V,Vongsouvath M,Davong V,Dance DAB

    更新日期:2018-06-01 00:00:00

  • Genotypic analysis of uropathogenic Escherichia coli to understand factors that impact the prevalence of β-lactam-resistant urinary tract infections in a community.

    abstract:OBJECTIVES:To determine the contribution of specific uropathogenic Escherichia coli (UPEC) lineages, drug resistance genes, and plasmid incompatibility/replicon (Inc) groups to the prevalence of β-lactam-resistant urinary tract infections (UTIs) in a university community. METHODS:Urine samples were consecutively colle...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2019.03.002

    authors: Tarlton NJ,Moritz C,Adams-Sapper S,Riley LW

    更新日期:2019-12-01 00:00:00

  • Detection of carbapenemase-producers: Evaluating the performance of the carbapenem inactivation method and Carba NP test versus multiplex PCR.

    abstract:OBJECTIVES:With a worrisome surge of carbapenem-resistant bacterial isolates, the diagnostic arsenal has become in dire need of affordable and timely assays to detect the rapidly transmissible carbapenemases. Employing multiplex PCR as a reference method, the purpose of the present study was to compare the performance ...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2016.12.015

    authors: Madkour LA,Soliman MS,Hassan DM,Soliman NS,ElMahdy YA

    更新日期:2017-06-01 00:00:00

  • Antimicrobial stewardship in hospitals: Does it work and can we do it?

    abstract::Selection of resistant pathogens by antimicrobial use is probably the most important cause of antimicrobial resistance. Antimicrobial stewardship (AMS) refers to a multifaceted approach to optimise prescribing. The benefits of AMS programmes have been widely demonstrated in terms of reductions in antimicrobial use, mo...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章,评审

    doi:10.1016/j.jgar.2013.08.001

    authors: Levy Hara G

    更新日期:2014-03-01 00:00:00

  • Complete genome sequencing and comparative plasmid analysis of KPC-2-producing Klebsiella pneumoniae isolated from hospital sewage water in Japan.

    abstract:OBJECTIVES:The Klebsiella pneumoniae carbapenemase (blaKPC) gene is one of the most widespread carbapenemase genes in the world. However, there are few reports on KPC-producing bacteria in Japan. The aim of this study was therefore to investigate KPC-producing K. pneumoniae in Japan. METHODS:A KPC-2-producingK. pneumo...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2020.12.007

    authors: Eda R,Maehana S,Hirabayashi A,Nakamura M,Furukawa T,Ikeda S,Sakai K,Kojima F,Sei K,Suzuki M,Kitasato H

    更新日期:2020-12-26 00:00:00

  • Emergence of an Escherichia coli strain co-harbouring mcr-1 and blaNDM-9 from a urinary tract infection in Taiwan.

    abstract:OBJECTIVES:Multidrug-resistant bacteria have become a serious threat worldwide. In particular, the coexistence of carbapenemase genes and mcr-1 leaves few available treatment options. Here we report a multidrug-resistant Escherichia coli isolate harbouring both mcr-1 and blaNDM-9 from a patient with a urinary tract inf...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2018.10.003

    authors: Lin YC,Kuroda M,Suzuki S,Mu JJ

    更新日期:2019-03-01 00:00:00

  • Genetic diversity and in vitro activity of ceftazidime/avibactam and aztreonam/avibactam against imipenem-resistant Enterobacteriaceae isolates in Southwest China: A single-centre study.

    abstract:OBJECTIVES:The aim of this study was to investigate the molecular mechanisms of imipenem resistance in Enterobacteriaceae and to assess the antimicrobial activities of ceftazidime/avibactam (CAZ/AVI) and aztreonam/avibactam (ATM/AVI) against imipenem-resistant clinical isolates in a tertiary hospital in China. METHODS...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2020.04.023

    authors: Wei J,Zou C,Wang D,Huang A,Niu S

    更新日期:2020-09-01 00:00:00

  • Analysis of isoniazid and rifampicin resistance in Mycobacterium tuberculosis isolates in Morocco using GenoType® MTBDRplus assay.

    abstract:OBJECTIVES:The number of multidrug-resistant tuberculosis (MDR-TB) cases is rising worldwide. The present investigation aimed to evaluate, using the GenoType® MTBDRplus assay, the most common mutations associated with rifampicin (RIF) and isoniazid (INH) resistance among resistant strains in Morocco. METHODS:A total o...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2017.09.017

    authors: Ennassiri W,Jaouhari S,Sabouni R,Cherki W,Charof R,Filali-Maltouf A,Lahlou O

    更新日期:2018-03-01 00:00:00

  • Long-range DHPS mutations unexpectedly increase Mycobacterium chimaera susceptibility to sulfonamides.

    abstract::The two closely related mycobacteria, Mycobacterium intracellulare and Mycobacterium chimaera, exhibit a more than two-fold difference in their in vitro susceptibility to sulfonamides. Sulfonamides are antibiotics targeting the 6-hydroxymethyl-7,8-dihydropteroate synthase (DHPS) enzyme involved in the folate synthesis...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2013.05.003

    authors: Gotthard G,Muhammed Ameen S,Drancourt M,Chabriere E

    更新日期:2013-12-01 00:00:00

  • Methicillin-resistant Staphylococcus aureus nasal colonisation amongst healthcare workers in Kurdistan Region, Iraq.

    abstract:OBJECTIVES:The aim of this study was to determine the prevalence of nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA) among healthcare workers (HCWs) compared with non-HCWs at Duhok city, Kurdistan Region, northern Iraq. METHODS:A total of 182 HCWs with different occupations and working in different...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2017.01.010

    authors: Hussein NR,Assafi MS,Ijaz T

    更新日期:2017-06-01 00:00:00

  • Pharmacokinetics of meropenem in burn patients with infections caused by Gram-negative bacteria: Are we getting close to the right treatment?

    abstract:OBJECTIVES:Infections caused by multidrug-resistant Gram-negative bacteria are associated with high mortality. A relevant concern is the efficacy of antibiotic therapy in burn patients in whom pathophysiological changes strongly influence pharmacokinetic (PK) parameters. This study aimed to describe the PK parameters o...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2019.06.011

    authors: Corcione S,D'Avolio A,Loia RC,Pensa A,Segala FV,De Nicolò A,Fatiguso G,Romeo M,Di Perri G,Stella M,De Rosa FG

    更新日期:2020-03-01 00:00:00

  • Draft genome sequence of an OXA-23, OXA-66, ADC-25 and TEM-1D co-producing Acinetobacter baumannii ST195 isolated from a patient with neonatal pneumonia in China.

    abstract:OBJECTIVES:The rapid spread of multidrug-resistant (MDR) Acinetobacter baumannii poses a substantial threat for morbidity and mortality worldwide. In particular, carbapenem-resistant A. baumannii has caused a severe challenge to human health. Here we reported the draft genome sequence of A. baumannii S131434, an OXA-23...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2018.11.008

    authors: Lv W,Zhang X,Hou M,Han D,Li Y,Xiong W

    更新日期:2019-03-01 00:00:00

  • Interaction of carbapenems and β-lactamase inhibitors towards CTX-M-15 and CTX-M-15G238C mutant.

    abstract:OBJECTIVES:The aim of this study was to evaluate the role of residue 238 in CTX-M-15 and CTX-M-15G238C mutant with respect to carbapenems and various β-lactamase inhibitors. METHODS:A CTX-M-15G238C laboratory mutant was generated by site-directed mutagenesis from CTX-M-15 enzyme by replacing glycine 238 with cysteine....

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2017.04.004

    authors: Sabatini A,Brisdelli F,Celenza G,Marcoccia F,Colapietro M,Tavío MM,Piccirilli A,Amicosante G,Perilli M

    更新日期:2017-09-01 00:00:00

  • Predictors of death among drug-resistant tuberculosis patients in Kuala Lumpur, Malaysia: A retrospective cohort study from 2009 to 2013.

    abstract::The emergence of drug-resistant tuberculosis (TB) is a major public health threat. However, little is known about the predictors of death in drug-resistant TB in Malaysia. This study aimed to determine the predictors of death in drug-resistant TB patients, including multidrug-resistant TB (MDR-TB), in Kuala Lumpur, Ma...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2016.04.005

    authors: Mohd Shariff N,Shah SA,Kamaludin F

    更新日期:2016-09-01 00:00:00

  • Complete genome arrangement revealed the emergence of a poultry origin superbug Citrobacter portucalensis strain NR-12.

    abstract:OBJECTIVES:Citrobacter spp. are part of normal human and animal intestinal flora. Citrobacter portucalensis (C. portucalensis) is closely related to Citrobacter freundii, which is an emerging opportunistic nosocomial pathogen. The aim of this study was to retrieve colistin-resistant Citrobacter spp. from poultry in Ban...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2019.05.031

    authors: Hasan MS,Sultana M,Hossain MA

    更新日期:2019-09-01 00:00:00

  • Genotypic and phylogenetic characterisation of a clinical Ralstonia pickettii strain carrying two novel OXA allelic variants, blaOXA-898 and blaOXA-899, isolated from a bloodstream infection in China.

    abstract:OBJECTIVES:Ralstonia pickettii has been increasingly recognised as an emerging opportunistic pathogen in hospital settings in recent years, especially in patients with prolonged hospital stay. Clinical manifestations associated with R. pickettii infection range from mild infections to severe invasive life-threatening i...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2020.02.020

    authors: Jiang T,Xu J,He F

    更新日期:2020-06-01 00:00:00