Characteristic antimicrobial resistance of clinically isolated Stenotrophomonas maltophilia CYZ via complete genome sequence.

Abstract:

OBJECTIVES:Stenotrophomonas maltophilia is an important multidrug-resistant pathogen that is associated with various serious nosocomial infections. In our study, we investigated the antimicrobial resistance traits of clinical S. maltophilia strain CYZ isolated from the sputum of an immunocompromised patient. METHODS:The whole genome sequence of S. maltophilia CYZ was investigated using a PacBio RS II system. The functions of all the predicted genes were annotated by the COG, GO and KEGG databases. Several types of antibiotics were selected to test the antimicrobial susceptibility, and a phylogenetic tree was constructed based on 16S rRNA gene sequence. RESULTS:The genome of S. maltophilia CYZ has a length of 4,517,685 bp and contains 4077 predicted genes, with an average G + C content of 66.65%. Functional genomic analysis via the annotations of the COG and GO databases revealed that the isolate exhibited specific means to resist antibiotics. The annotated genes involved in flagella, pili or fimbriae, biofilm formation, polysaccharide and cyclic di-GMP may contribute to promote the ability of antimicrobial resistance. This strain showed susceptibility to levofloxacin, trimethoprim/sulfamethoxazole and minocycline according to antimicrobial susceptibility testing. The phylogenetic relationship indicated that S. maltophilia CYZ was closely related to S. maltophilia strains isolated from the nosocomial environment. CONCLUSIONS:The current results give a better understanding of the genetic characteristics of antimicrobial resistance in S. maltophilia CYZ and provide a genetic basis for further study of the phenotype.

authors

Ma J,Feng J,Shan Y,Zhao Y,Qiao H,Xie L,Lin X,Wang C,Chuai X

doi

10.1016/j.jgar.2020.09.008

subject

Has Abstract

pub_date

2020-12-01 00:00:00

pages

186-193

eissn

2213-7165

issn

2213-7173

pii

S2213-7165(20)30239-3

journal_volume

23

pub_type

杂志文章
  • Impact of ethnic disparities on the treatment outcomes of HIV-negative drug-resistant tuberculosis patients in Kuala Lumpur, Malaysia: A call for a culturally-sensitive community intervention approach.

    abstract:OBJECTIVE:Little is known about the treatment outcomes of HIV-negative drug-resistant tuberculosis (TB) patients in Malaysia. With respect to this issue, this study aimed to determine factors associated with unsuccessful treatment outcomes among drug-resistant TB patients at the Institute of Respiratory Medicine, Kuala...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2019.05.009

    authors: Shariff NM,Shah SA,Kamaludin F

    更新日期:2019-12-01 00:00:00

  • Antibiotic susceptibility and molecular epidemiology of Acinetobacter calcoaceticus-baumannii complex strains isolated from a referral hospital in northern Vietnam.

    abstract::Acinetobacter calcoaceticus-baumannii complex is a common cause of hospital-acquired infections (HAIs) globally, remarkable for its high rate of antibiotic resistance, including to carbapenems. There are few data on the resistance of A. baumannii in Vietnam, which are essential for developing evidence-based treatment ...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2014.05.003

    authors: Van TD,Dinh QD,Vu PD,Nguyen TV,Pham CV,Dao TT,Phung CD,Hoang HT,Tang NT,Do NT,Nguyen KV,Wertheim H

    更新日期:2014-12-01 00:00:00

  • Detection of carbapenemase-producers: Evaluating the performance of the carbapenem inactivation method and Carba NP test versus multiplex PCR.

    abstract:OBJECTIVES:With a worrisome surge of carbapenem-resistant bacterial isolates, the diagnostic arsenal has become in dire need of affordable and timely assays to detect the rapidly transmissible carbapenemases. Employing multiplex PCR as a reference method, the purpose of the present study was to compare the performance ...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2016.12.015

    authors: Madkour LA,Soliman MS,Hassan DM,Soliman NS,ElMahdy YA

    更新日期:2017-06-01 00:00:00

  • Draft genome sequences of two fluoroquinolone-resistant CTX-M-15-producing Escherichia coli ST90 (ST23 complex) isolated from a calf and a dairy cow in South America.

    abstract:OBJECTIVES:Farm animals have been recognised as important carriers and reservoirs of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli. The aim of this study was to report the draft genome sequences of two multidrug-resistant (MDR) CTX-M-15-producing E. coli strains (47VL and 13B) isolated from different ...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2017.10.009

    authors: Sartori L,Fernandes MR,Ienne S,de Souza TA,Gregory L,Cerdeira L,Lincopan N

    更新日期:2017-12-01 00:00:00

  • Coexistence of three blaKPC-2 genes on an IncF/IncR plasmid in ST11 Klebsiella pneumoniae.

    abstract:OBJECTIVES:Here we report the finding of three copies of the blaKPC-2 gene on a plasmid in ST11 Klebsiella pneumoniae. METHODS:Carbapenem-resistant K. pneumoniae clinical strain WCHKP2 was subjected to whole-genome sequencing (WGS) using both a short-read Illumina HiSeq X10 platform and long-read MinION sequencer. Hyb...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2018.11.017

    authors: Feng Y,Liu L,McNally A,Zong Z

    更新日期:2019-06-01 00:00:00

  • Meropenem pharmacokinetics during extracorporeal membrane oxygenation and continuous haemodialysis: a case report.

    abstract:OBJECTIVES:Pharmacokinetic (PK) parameters can change significantly during extracorporeal membrane oxygenation (ECMO) and continuous haemodialysis. This case report describes the pharmacokinetics of a 3-h meropenem infusion in an infantile anuric patient on ECMO with continuous haemodialysis. CASE:A 19-month-old femal...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2020.04.029

    authors: Saito J,Shoji K,Oho Y,Aoki S,Matsumoto S,Yoshida M,Nakamura H,Kaneko Y,Hayashi T,Yamatani A,Capparelli E,Miyairi I

    更新日期:2020-09-01 00:00:00

  • Plasmid metagenomics reveals multiple antibiotic resistance gene classes among the gut microbiomes of hospitalised patients.

    abstract::Antibiotic resistance genes are rapidly spread between pathogens and the normal flora, with plasmids playing an important role in their circulation. This study aimed to investigate antibiotic resistance plasmids in the gut microbiome of hospitalised patients. Stool samples were collected from seven inpatients at Sirir...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2016.03.001

    authors: Jitwasinkul T,Suriyaphol P,Tangphatsornruang S,Hansen MA,Hansen LH,Sørensen SJ,Permpikul C,Rongrungruang Y,Tribuddharat C

    更新日期:2016-09-01 00:00:00

  • Resistance mechanisms and clinical characteristics of linezolid-resistant Enterococcus faecium isolates: A single-centre study in South Korea.

    abstract:OBJECTIVES:This study aimed to determine the prevalence of linezolid-resistant (LR) vancomycin-resistant enterococci and to investigate the mechanisms of linezolid resistance with clinical and microbiological characterisation. METHODS:All vancomycin-resistant Enterococcus faecium (VREF) isolated from blood and rectal ...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2017.09.009

    authors: Cho SY,Kim HM,Chung DR,Kim SH,Huh HJ,Kang CI,Peck KR,Lee NY,Song JH

    更新日期:2018-03-01 00:00:00

  • Analysis of isoniazid and rifampicin resistance in Mycobacterium tuberculosis isolates in Morocco using GenoType® MTBDRplus assay.

    abstract:OBJECTIVES:The number of multidrug-resistant tuberculosis (MDR-TB) cases is rising worldwide. The present investigation aimed to evaluate, using the GenoType® MTBDRplus assay, the most common mutations associated with rifampicin (RIF) and isoniazid (INH) resistance among resistant strains in Morocco. METHODS:A total o...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2017.09.017

    authors: Ennassiri W,Jaouhari S,Sabouni R,Cherki W,Charof R,Filali-Maltouf A,Lahlou O

    更新日期:2018-03-01 00:00:00

  • Individual feedback to reduce inappropriate antimicrobial prescriptions for treating acute upper respiratory infections in an outpatient setting of a Thai university hospital.

    abstract:OBJECTIVES:Excessive use of antimicrobial agents for acute upper respiratory infections (URIs) is one of the most common problems in clinical practice. Interventions are required to reduce antimicrobial overuse in these common infections. METHODS:During a 9-month study period (June 2014-February 2015), a quasi-experim...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2017.08.015

    authors: Rattanaumpawan P,Chuenchom N,Thamlikitkul V,Siriraj Antimicrobial Stewardship Program.

    更新日期:2018-03-01 00:00:00

  • Characterisation of a novel integrative and conjugative element ICESsD9 carrying erm(B) and tet(O) resistance determinants in Streptococcus suis, and the distribution of ICESsD9-like elements in clinical isolates.

    abstract::This study identified a novel integrative and conjugative element (ICESsD9) carrying erm(B) and tet(O) resistance determinants in Streptococcus suis D9 and determined its prevalence in clinical isolates. Comparative genome analysis was performed using Mauve and Artemis Comparison Tool visualisation programs. Inverse P...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2016.05.008

    authors: Huang K,Song Y,Zhang Q,Zhang A,Jin M

    更新日期:2016-12-01 00:00:00

  • Forecasting models of infections due to carbapenem-resistant Gram-negative bacteria in an intensive care unit in an endemic area.

    abstract:OBJECTIVES:The aim of this study was to forecast the monthly incidence rates of infections [infections/1000 bed-days (IBD)] due to carbapenem-resistant Klebsiella pneumoniae (CRKP), carbapenem-resistant Pseudomonas aeruginosa (CRPA), carbapenem-resistant Acinetobacter baumannii (CRAB) and total carbapenem-resistant Gra...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2019.06.019

    authors: Karampatakis T,Tsergouli K,Iosifidis E,Antachopoulos C,Mouloudi E,Karyoti A,Tsakris A,Roilides E

    更新日期:2020-03-01 00:00:00

  • Antibiotic use in food animals worldwide, with a focus on Africa: Pluses and minuses.

    abstract::Antibiotics are frequently used in food animal production in developing countries to promote the well-being and growth of animals. This practice provides some economic benefits to producers and consumers at large. Nevertheless, this practice is also associated with a number of concerns. A major concern has been that r...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章,评审

    doi:10.1016/j.jgar.2019.07.031

    authors: Van TTH,Yidana Z,Smooker PM,Coloe PJ

    更新日期:2020-03-01 00:00:00

  • Global antibacterial resistance: The never-ending story.

    abstract::Bacterial resistance is undoubtedly recognised as a major medical challenge in most healthcare systems. Resistance-determining genes, mostly in combination, and multidrug-resistant (MDR) pathogens are spreading with unprecedented speed. Well known resistance carriers with high clinical impact include the Gram-positive...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章,评审

    doi:10.1016/j.jgar.2013.03.010

    authors: Theuretzbacher U

    更新日期:2013-06-01 00:00:00

  • Draft genome sequence of a multidrug-resistant KPC-2-producing Enterobacter aerogenes isolated from a hospitalised patient in Brazil.

    abstract:OBJECTIVES:Multidrug-resistant (MDR) Enterobacter aerogenes strains are frequently associated with nosocomial infections and high mortality rates, representing a serious public health problem. The aim of this study was to present the draft genome sequence of a MDR KPC-2-producing E. aerogenes isolated from a perineal s...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2017.07.016

    authors: Moura Q,Fernandes MR,Cerdeira L,Nhambe LF,Ienne S,Souza TA,Lincopan N

    更新日期:2017-09-01 00:00:00

  • Draft genome sequence of ST203 vanA Enterococcus faecium strain XH877 from China.

    abstract::Enterococcus faecium was considered an important nosocomial pathogen that could incur a substantial health burden owing to its high drug resistance and its ability to adapt to the hospital niche. Here we presented the draft genome sequence of multidrug-resistant E. faecium XH877. The draft genome sequence was composed...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2016.07.004

    authors: Hua X,Sun L,Shi K,Yu Y

    更新日期:2016-12-01 00:00:00

  • Draft genome sequence of an OXA-23, OXA-66, ADC-25 and TEM-1D co-producing Acinetobacter baumannii ST195 isolated from a patient with neonatal pneumonia in China.

    abstract:OBJECTIVES:The rapid spread of multidrug-resistant (MDR) Acinetobacter baumannii poses a substantial threat for morbidity and mortality worldwide. In particular, carbapenem-resistant A. baumannii has caused a severe challenge to human health. Here we reported the draft genome sequence of A. baumannii S131434, an OXA-23...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2018.11.008

    authors: Lv W,Zhang X,Hou M,Han D,Li Y,Xiong W

    更新日期:2019-03-01 00:00:00

  • Whole genome sequence of an Escherichia coli ST410 isolate co-harbouring blaNDM-5, blaOXA-1, blaCTX-M-15, blaCMY-2, aac(3)-IIa and aac(6')-Ib-cr genes isolated from a patient with bloodstream infection in China.

    abstract:OBJECTIVES:Mortality associated with carbapenemase-producing Enterobacteriaceae is high as there are few therapeutic options. Escherichia coli sequence type 410 (ST410) is currently an international high-risk clone and is responsible for a large number of clinical infections. Here we report the draft genome sequence of...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2019.10.027

    authors: Huang J,Ma S,Yu Q,Fu M,Shao L,Shan X,Li X

    更新日期:2019-12-01 00:00:00

  • Genotypic analysis of uropathogenic Escherichia coli to understand factors that impact the prevalence of β-lactam-resistant urinary tract infections in a community.

    abstract:OBJECTIVES:To determine the contribution of specific uropathogenic Escherichia coli (UPEC) lineages, drug resistance genes, and plasmid incompatibility/replicon (Inc) groups to the prevalence of β-lactam-resistant urinary tract infections (UTIs) in a university community. METHODS:Urine samples were consecutively colle...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2019.03.002

    authors: Tarlton NJ,Moritz C,Adams-Sapper S,Riley LW

    更新日期:2019-12-01 00:00:00

  • Carbapenem-resistant Klebsiella pneumoniae infections in a Greek intensive care unit: Molecular characterisation and treatment challenges.

    abstract::Acquisition of carbapenemase-producing Klebsiella pneumoniae (CP-Kp) strains poses a major threat to critically ill patients. The objectives of this study were to describe the epidemiology of CP-Kp isolates as well as the clinical outcome associated with the corresponding infections and to identify risk factors for mo...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2015.01.006

    authors: Katsiari M,Panagiota G,Likousi S,Roussou Z,Polemis M,Alkiviadis Vatopoulos C,Evangelia Platsouka D,Maguina A

    更新日期:2015-06-01 00:00:00

  • Draft genome sequence of a Kluyvera intermedia isolate from a patient with a pancreatic abscess.

    abstract::The genus Kluyvera comprises potential pathogens that can cause many infections. This study reports a Kluyvera intermedia strain (FOSA7093) from a pancreatic cyst specimen from a long-term hospitalised patient. Whole-genome sequencing (WGS) of the K. intermedia isolate was performed and the strain was reported as sens...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2017.05.007

    authors: Thele R,Gumpert H,Christensen LB,Worning P,Schønning K,Westh H,Hansen TA

    更新日期:2017-09-01 00:00:00

  • Possible application of high-dose vitamin C in the prevention and therapy of coronavirus infection.

    abstract::Coronaviruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza viruses increase oxidative stress in the body leading to cellular and tissue damage. To combat this, administration of high-dose vitamin C (ascorbic acid or ascorbate), in addition to standard conventional supportive treat...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章,评审

    doi:10.1016/j.jgar.2020.09.025

    authors: Hoang BX,Shaw G,Fang W,Han B

    更新日期:2020-12-01 00:00:00

  • Long-range DHPS mutations unexpectedly increase Mycobacterium chimaera susceptibility to sulfonamides.

    abstract::The two closely related mycobacteria, Mycobacterium intracellulare and Mycobacterium chimaera, exhibit a more than two-fold difference in their in vitro susceptibility to sulfonamides. Sulfonamides are antibiotics targeting the 6-hydroxymethyl-7,8-dihydropteroate synthase (DHPS) enzyme involved in the folate synthesis...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2013.05.003

    authors: Gotthard G,Muhammed Ameen S,Drancourt M,Chabriere E

    更新日期:2013-12-01 00:00:00

  • Interaction of carbapenems and β-lactamase inhibitors towards CTX-M-15 and CTX-M-15G238C mutant.

    abstract:OBJECTIVES:The aim of this study was to evaluate the role of residue 238 in CTX-M-15 and CTX-M-15G238C mutant with respect to carbapenems and various β-lactamase inhibitors. METHODS:A CTX-M-15G238C laboratory mutant was generated by site-directed mutagenesis from CTX-M-15 enzyme by replacing glycine 238 with cysteine....

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2017.04.004

    authors: Sabatini A,Brisdelli F,Celenza G,Marcoccia F,Colapietro M,Tavío MM,Piccirilli A,Amicosante G,Perilli M

    更新日期:2017-09-01 00:00:00

  • Epidemiology of multidrug-resistant Gram-negative pathogens isolated from ventilator-associated pneumonia in ICU patients.

    abstract:OBJECTIVES:Antibiotic resistance among Gram-negative pathogens isolated from ventilator-associated pneumonia (VAP) poses a grave threat in intensive care unit (ICU) patients. The aim of this study was to assess the prevalence of pathogens in ICU patients and their drug resistance profile. The prevalence of extended-spe...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2016.12.016

    authors: Gupta R,Malik A,Rizvi M,Ahmed M,Singh A

    更新日期:2017-06-01 00:00:00

  • Genetic characterisation of a whiB7 mutant of a Mycobacterium tuberculosis clinical strain.

    abstract::Mycobacterium tuberculosis is naturally resistant to clarithromycin (CLR). The genes Rv3197A (whiB7) and Rv1988 (ermMT) have been shown to be involved in the resistant phenotype. In this study, a CLR-susceptible M. tuberculosis clinical strain was identified, designated as DS3214, and the nucleotide sequences and expr...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2015.07.004

    authors: Warit S,Phunpruch S,Jityam C,Jaitrong S,Billamas P,Chaiprasert A,Palittapongarnpim P,Prammananan T

    更新日期:2015-12-01 00:00:00

  • New chalcone compound as a promising antileishmanial drug for an old neglected disease: Biological evaluation using radiolabelled biodistribution.

    abstract:OBJECTIVES:Treatment of leishmaniasis remains a challenge, especially due to the need for multiple painful injections, the toxicity of current drugs against the disease, their lack of efficacy and, lately, drug resistance. The aim of this study was to demonstrate the biological behaviour of 3-nitro-2'-hydroxy-4',6'-dim...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2017.11.012

    authors: Sousa-Batista AJ,Philipon CIMS,de Souza Albernaz M,Pinto SR,Rossi-Bergmann B,Santos-Oliveira R

    更新日期:2018-06-01 00:00:00

  • Update on the activity of delafloxacin against acute bacterial skin and skin-structure infection isolates from European hospitals (2014-2019).

    abstract:OBJECTIVES:Delafloxacin is a broad-spectrum anionic fluoroquinolone with activity against Gram-positive and Gram-negative organisms, including methicillin-resistant Staphylococcus aureus. Both oral and intravenous formulations were approved for use in the treatment of acute bacterial skin and skin-structure infections ...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2020.09.027

    authors: Shortridge D,Pfaller MA,Streit JM,Flamm RK

    更新日期:2020-12-01 00:00:00

  • Draft genome sequences of three multidrug-resistant Cutibacterium (formerly Propionibacterium) acnes strains isolated from acne patients, China.

    abstract:OBJECTIVES:Cutibacterium acnes (formerly Propionibacterium acnes), an anaerobic Gram-positive bacterium, is a skin commensal and is considered to be associated with acne. Here we report the draft genome sequences of three multidrug-resistant (MDR) C. acnes strains (CA17, CA39 and CA51) isolated from skin lesions of acn...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2017.10.008

    authors: Zhang N,Lu Z,Ma Y

    更新日期:2017-12-01 00:00:00

  • Antimicrobial resistance patterns of uropathogens isolated between 2012 and 2017 from a tertiary hospital in Northern Ethiopia.

    abstract:OBJECTIVES:Urinary tract infections (UTIs) are one of the most common infections in humans. Studies have shown that antibiotics for UTIs are usually prescribed empirically before the results of urine culture are available. The aim of the study was to assess the antimicrobial resistance patterns of bacteria isolated fro...

    journal_title:Journal of global antimicrobial resistance

    pub_type: 杂志文章

    doi:10.1016/j.jgar.2019.01.022

    authors: Tuem KB,Desta R,Bitew H,Ibrahim S,Hishe HZ

    更新日期:2019-09-01 00:00:00