Analysis of isoniazid and rifampicin resistance in Mycobacterium tuberculosis isolates in Morocco using GenoType® MTBDRplus assay.

Abstract:

OBJECTIVES:The number of multidrug-resistant tuberculosis (MDR-TB) cases is rising worldwide. The present investigation aimed to evaluate, using the GenoType® MTBDRplus assay, the most common mutations associated with rifampicin (RIF) and isoniazid (INH) resistance among resistant strains in Morocco. METHODS:A total of 319 Mycobacterium tuberculosis isolates sent to the National Tuberculosis Reference Laboratory between 2013 and 2015 were subjected to GenoType® MTBDRplus for detecting M. tuberculosis and determination of drug susceptibility. Correlation of the minimum inhibitory concentrations (MICs) of INH with genotypic assay results was carried out for 97 MDR-TB strains. Various concentrations of INH were tested. RESULTS:The most frequent mutations observed were rpoBS531L (67.2%) and katGS315T1/2 (66.5%). Isolates with inhA gene mutation, katG gene mutation, and dual mutations in katG and inhA had MICs ranging from 0.5-1μg/mL, 2-10μg/mL and ≥12μg/mL, respectively. CONCLUSION:In Morocco, 66.5% and 76.7% of M. tuberculosis strains carried mutations causing high-level resistance to INH and RIF, respectively.

authors

Ennassiri W,Jaouhari S,Sabouni R,Cherki W,Charof R,Filali-Maltouf A,Lahlou O

doi

10.1016/j.jgar.2017.09.017

subject

Has Abstract

pub_date

2018-03-01 00:00:00

pages

197-201

eissn

2213-7165

issn

2213-7173

pii

S2213-7165(17)30183-2

journal_volume

12

pub_type

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