当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Dynamics of living cells in a cytomorphological state space.

Dynamics of living cells in a cytomorphological state space.

Abstract：

:Cells are nonequilibrium systems that exchange matter and energy with the environment to sustain their metabolic needs. The nonequilibrium nature of this system presents considerable challenges to developing a general theory describing its behavior; however, when studied at appropriate spatiotemporal scales, the behavior of ensembles of nonequilibrium systems can resemble that of a system at equilibrium. Here we apply this principle to a population of cells within a cytomorphological state space and demonstrate that cellular transition dynamics within this space can be described using equilibrium formalisms. We use this framework to map the effective energy landscape underlying the cytomorphological state space of a population of mouse embryonic fibroblasts (MEFs) and identify topographical nonuniformity in this space, indicating nonuniform occupation of cytomorphological states within an isogenic population. The introduction of exogenous apoptotic agents fundamentally altered this energy landscape, inducing formation of additional energy minima that correlated directly with changes in sensitivity to apoptosis induction. An equilibrium framework allows us to describe the behavior of an ensemble of single cells, suggesting that although cells are complex nonequilibrium systems, the application of formalisms derived from equilibrium thermodynamics can provide insight into the basis of nongenetic heterogeneities within cell populations, as well as the relationship between cytomorphological and functional heterogeneity.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Chang AY,Marshall WF

doi

10.1073/pnas.1902849116

subject

Has Abstract

pub_date

2019-10-22 00:00:00

pages

21556-21562

issue

43

eissn

0027-8424

issn

1091-6490

pii

1902849116

journal_volume

116

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Distinguishing surface effects of calcium ion from pore-occupancy effects in Na+ channels.

    abstract：:The effects of calcium ion on the Na+ activation gate were studied in squid giant axons. Saxitoxin (STX) was used to block ion entry into Na+ channels without hindering access to the membrane surface, making it possible to distinguish surface effects of calcium from pore-occupancy effects. In the presence of STX, gati...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.7.4158

    authors： Armstrong CM

    更新日期：1999-03-30 00:00:00

  • Early effect of interferon on mouse leukemia cells cultivated in a chemostat.

    abstract：:Mouse interferon preparations inhibited the multiplication of mouse leukemia L 1210 cells cultivated under steady-state conditions in a chemostat. The use of this sensitive and controlled system led to the detection of a rapid inhibition in the incorporation of (3-H)thymidine into cellular acid-precipitable material 2...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.6.2265

    authors： Tovey M,Brouty-Boyé D,Gresser I

    更新日期：1975-06-01 00:00:00

  • The active site dynamics of 4-chlorobenzoyl-CoA dehalogenase.

    abstract：:A molecular dynamics study was performed to compare the differences in the active-site dynamics of the wild-type and W137F mutant enzymes of 4-chlorobenzoyl-CoA dehalogenase. Only in the wild-type simulation are conformations formed between the catalytic Asp-145 and 4-chlorobenzoyl-CoA, which resemble the ab initio ca...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.161282698

    authors： Lau EY,Bruice TC

    更新日期：2001-08-14 00:00:00

  • FMS mutations in myelodysplastic, leukemic, and normal subjects.

    abstract：:The FMS gene encodes the functional cell surface receptor for colony-stimulating factor 1, the macrophage- and monocyte-specific growth factor. Codons 969 and 301 have been identified as potentially involved in promoting the transforming activity of FMS. Mutations at codon 301 are believed to lead to neoplastic transf...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.4.1377

    authors： Ridge SA,Worwood M,Oscier D,Jacobs A,Padua RA

    更新日期：1990-02-01 00:00:00

  • Characterization of cultured rat oligodendrocytes proliferating in a serum-free, chemically defined medium.

    abstract：:A serumless, chemically defined medium has been developed for the culture of oligodendrocytes isolated from primary neonatal rat cerebral cultures. Combined together, insulin, transferrin, and fibroblast growth factor synergistically induced an essentially homogenous population (95-98%) of cells expressing glycerol-3-...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.10.3509

    authors： Saneto RP,de Vellis J

    更新日期：1985-05-01 00:00:00

  • Adaptive evolution of cytochrome c oxidase subunit VIII in anthropoid primates.

    abstract：:Cytochrome c oxidase (COX) is a 13-subunit protein complex that catalyzes the last step in mitochondrial electron transfer in mammals. Of the 10 subunits encoded by nuclear DNA (three are mtDNA products), some are expressed as tissue- and/or development-specific isoforms. For COX subunit VIII, previous work showed tha...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0931463100

    authors： Goldberg A,Wildman DE,Schmidt TR,Huttemann M,Goodman M,Weiss ML,Grossman LI

    更新日期：2003-05-13 00:00:00

  • Dual activation of a sex pheromone-dependent ion channel from insect olfactory dendrites by protein kinase C activators and cyclic GMP.

    abstract：:Olfactory transduction is thought to take place in the outer dendritic membrane of insect olfactory receptor neurons. Here we show that the outer dendritic plasma membrane of silkmoth olfactory receptor neurons seems to be exclusively equipped with a specific ion channel activated by low concentrations of the species-...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.19.8520

    authors： Zufall F,Hatt H

    更新日期：1991-10-01 00:00:00

  • Model for specific rescue of normal hepatocytes during methotrexate treatment of hepatic malignancy.

    abstract：:We utilized the presence of hepatic receptors for galactose-terminal glycoproteins to achieve specific rescue of differentiated hepatocytes from methotrexate toxicity. This was accomplished by administration of a conjugate formed by covalent coupling of the methotrexate antagonist, folinic acid (leucovorin), to galact...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.80.10.3078

    authors： Wu GY,Wu CH,Stockert RJ

    更新日期：1983-05-01 00:00:00

  • Pressure-induced phase transitions and superconductivity in a black phosphorus single crystal.

    abstract：:We report a thorough study of the transport properties of the normal and superconducting states of black phosphorus (BP) under magnetic field and high pressure with a large-volume apparatus that provides hydrostatic pressure to induce transitions from the layered A17 phase to the layered A7 phase and to the cubic phas...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1810726115

    authors： Li X,Sun J,Shahi P,Gao M,MacDonald AH,Uwatoko Y,Xiang T,Goodenough JB,Cheng J,Zhou J

    更新日期：2018-10-02 00:00:00

  • Inverted repeat structure of the Sry locus in mice.

    abstract：:The testis-determining gene Sry is located on the short arm of the mouse Y chromosome in a region known to have undergone duplications and rearrangements in comparison with the equivalent portion of the human Y chromosome. Detailed analysis of the Sry genomic locus reveals a further difference in that the mouse Sry op...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.17.7953

    authors： Gubbay J,Vivian N,Economou A,Jackson D,Goodfellow P,Lovell-Badge R

    更新日期：1992-09-01 00:00:00

  • Ca2+ signaling by plant Arabidopsis thaliana Pep peptides depends on AtPepR1, a receptor with guanylyl cyclase activity, and cGMP-activated Ca2+ channels.

    abstract：:A family of peptide signaling molecules (AtPeps) and their plasma membrane receptor AtPepR1 are known to act in pathogen-defense signaling cascades in plants. Little is currently known about the molecular mechanisms that link these signaling peptides and their receptor, a leucine-rich repeat receptor-like kinase, to d...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1000191107

    authors： Qi Z,Verma R,Gehring C,Yamaguchi Y,Zhao Y,Ryan CA,Berkowitz GA

    更新日期：2010-12-07 00:00:00

  • Accurately estimating neuronal correlation requires a new spike-sorting paradigm.

    abstract：:Neurophysiology is increasingly focused on identifying coincident activity among neurons. Strong inferences about neural computation are made from the results of such studies, so it is important that these results be accurate. However, the preliminary step in the analysis of such data, the assignment of spike waveform...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1115236109

    authors： Ventura V,Gerkin RC

    更新日期：2012-05-08 00:00:00

  • As4S4 targets RING-type E3 ligase c-CBL to induce degradation of BCR-ABL in chronic myelogenous leukemia.

    abstract：:Arsenic, a curative agent for acute promyelocytic leukemia, induces cell apoptosis and degradation of BCR-ABL in chronic myelogenous leukemia (CML). We demonstrated that ubiquitination and degradation of BCR-ABL was mediated by c-CBL, a RING-type E3 ligase that was also shown to be involved in ubiquitination for many ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1016311108

    authors： Mao JH,Sun XY,Liu JX,Zhang QY,Liu P,Huang QH,Li KK,Chen Q,Chen Z,Chen SJ

    更新日期：2010-12-14 00:00:00

  • Potent inhibition of huntingtin aggregation and cytotoxicity by a disulfide bond-free single-domain intracellular antibody.

    abstract：:Huntington's disease (HD) is a progressive neurodegenerative disorder caused by an expansion in the number of polyglutamine-encoding CAG repeats in the gene that encodes the huntingtin (htt) protein. A property of the mutant protein that is intimately involved in the development of the disease is the propensity of the...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0408134101

    authors： Colby DW,Chu Y,Cassady JP,Duennwald M,Zazulak H,Webster JM,Messer A,Lindquist S,Ingram VM,Wittrup KD

    更新日期：2004-12-21 00:00:00

  • Immunohistochemistry of HLA-H, the protein defective in patients with hereditary hemochromatosis, reveals unique pattern of expression in gastrointestinal tract.

    abstract：:Hereditary hemochromatosis (HH) is a common autosomal recessive disorder of iron metabolism that leads to excessive iron storage in the liver and other organs. Recently, between 83 and 100% of HH patients have been found to be homozygous for the same mutation in a novel major histocompatibility complex class I-like ge...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.6.2534

    authors： Parkkila S,Waheed A,Britton RS,Feder JN,Tsuchihashi Z,Schatzman RC,Bacon BR,Sly WS

    更新日期：1997-03-18 00:00:00

  • Increased cell proliferation, but not reduced cell death, induces lymphocytosis in bovine leukemia virus-infected sheep.

    abstract：:Lymphocyte homeostasis is the result of a critical balance between cell proliferation and death. Disruption of this subtle equilibrium can lead to the onset of leukemia, an increase in the number of lymphocytes being potentially due to both of these parameters. The relative importance of cell proliferation vs. apoptos...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.142100999

    authors： Debacq C,Asquith B,Kerkhofs P,Portetelle D,Burny A,Kettmann R,Willems L

    更新日期：2002-07-23 00:00:00

  • Optimal prediction of stiff oscillatory mechanics.

    abstract：:We consider many-body problems in classical mechanics where a wide range of time scales limits what can be computed. We apply the method of optimal prediction to obtain equations that are easier to solve numerically. We demonstrate by examples that optimal prediction can reduce the amount of computation needed to obta...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.97.12.6253

    authors： Kast AP

    更新日期：2000-06-06 00:00:00

  • Spiral structure of Escherichia coli HUalphabeta provides foundation for DNA supercoiling.

    abstract：:We determined the crystal structure of the Escherichia coli nucleoid-associated HUalphabeta protein by x-ray diffraction and observed that the heterodimers form multimers with octameric units in three potential arrangements, which may serve specialized roles in different DNA transaction reactions. It is of special imp...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0611686104

    authors： Guo F,Adhya S

    更新日期：2007-03-13 00:00:00

  • Cerebellar GABAergic processes: evidence for critical involvement in a form of simple associative learning in the rabbit.

    abstract：:Converging evidence from electrophysiological recording and lesion studies suggests an essential role for the cerebellum in classical conditioning of the nictitating membrane response in the rabbit. The present study begins to delineate within this structure neurotransmitter systems that appear critical for the expres...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.7.2101

    authors： Mamounas LA,Thompson RF,Madden J 4th

    更新日期：1987-04-01 00:00:00

  • Combined deficiency for MAP kinase-interacting kinase 1 and 2 (Mnk1 and Mnk2) delays tumor development.

    abstract：:MAP kinase-interacting kinase 1 and 2 (Mnk1 and Mnk2) are protein-serine/threonine kinases that are activated by ERK or p38 and phosphorylate eIF4E, which is involved in cap-dependent translation initiation. However, Mnk1/2 double knockout (Mnk-DKO) mice show normal cell growth and development despite an absence of eI...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1008136107

    authors： Ueda T,Sasaki M,Elia AJ,Chio II,Hamada K,Fukunaga R,Mak TW

    更新日期：2010-08-10 00:00:00

  • Massive targeting of liposomes, surface-modified with anionized albumins, to hepatic endothelial cells.

    abstract：:Human serum albumin (HSA) derivatized with cis-aconitic anhydride was covalently coupled to liposomes with a size of approximately 100 nm [polyaconitylated HSA (Aco-HSA) liposomes]. Within 30 min after injection into a rat, Aco-HSA liposomes were completely cleared from the blood and almost exclusively taken up by the...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.21.11681

    authors： Kamps JA,Morselt HW,Swart PJ,Meijer DK,Scherphof GL

    更新日期：1997-10-14 00:00:00

  • Translation of mRNAs with degenerate initiation triplet AUU displays high initiation factor 2 dependence and is subject to initiation factor 3 repression.

    abstract：:The influence of the rare initiation triplet AUU on mRNA translation was investigated by comparing the activity of two pairs of model mRNAs that differ in the length of Shine-Dalgarno and spacer sequences. Irrespective of the initiation triplet (AUG or AUU), all mRNAs had similar template activity in vitro, but transl...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.9.4161

    authors： La Teana A,Pon CL,Gualerzi CO

    更新日期：1993-05-01 00:00:00

  • Neoantigens of the membrane attack complex of human complement.

    abstract：:The membrane attack complex of complement is a fusion product of five complement proteins: C5b, C6, C7, C8, and C9. The complex causes complement-dependent cell membrane damage. It is assembled following complement activation both on the target cell surface and in the fluid phase. The isolated soluble complex, which h...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.5.1687

    authors： Kolb WP,Müller-Eberhard HJ

    更新日期：1975-05-01 00:00:00

  • Role of cyclophilin A in the uptake of HIV-1 by macrophages and T lymphocytes.

    abstract：:Cyclophilins are a family of proteins that bind cyclosporin A (CsA) and possess peptidyl-prolyl cis-trans isomerase activity. In addition, they are secreted by activated cells and act in a cytokine-like manner, presumably via signaling through a cell surface cyclophilin receptor. More recently, host-derived cyclophili...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.4.1758

    authors： Sherry B,Zybarth G,Alfano M,Dubrovsky L,Mitchell R,Rich D,Ulrich P,Bucala R,Cerami A,Bukrinsky M

    更新日期：1998-02-17 00:00:00

  • A library of bacteriophage-displayed antibody fragments directed against proteins of the inner ear.

    abstract：:Bacteriophage display of antibodies provides a method for the generation of immunological reagents against rare and uncharacterized antigens. To ascertain the usefulness of this approach for the characterization of inner-ear proteins, we produced a bacteriophage-displayed antibody-fragment library directed against pro...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.030535797

    authors： Cyr JL,Hudspeth AJ

    更新日期：2000-02-29 00:00:00

  • Inhibition of retinoic acid-induced activation of 3' human HOXB genes by antisense oligonucleotides affects sequential activation of genes located upstream in the four HOX clusters.

    abstract：:Most homeobox genes belonging to the Hox family are sequentially activated in embryonal carcinoma cells upon treatment with retinoic acid. Genes located at the 3' end of each one of the four Hox clusters are activated first, whereas upstream Hox genes are activated progressively later. This activation has been extensi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.12.5335

    authors： Faiella A,Zappavigna V,Mavilio F,Boncinelli E

    更新日期：1994-06-07 00:00:00

  • P-hacking in clinical trials and how incentives shape the distribution of results across phases.

    abstract：:Clinical research should conform to high standards of ethical and scientific integrity, given that human lives are at stake. However, economic incentives can generate conflicts of interest for investigators, who may be inclined to withhold unfavorable results or even tamper with data in order to achieve desired outcom...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1919906117

    authors： Adda J,Decker C,Ottaviani M

    更新日期：2020-06-16 00:00:00

  • Mg2+ mediates interaction between the voltage sensor and cytosolic domain to activate BK channels.

    abstract：:The voltage-sensor domain (VSD) of voltage-dependent ion channels and enzymes is critical for cellular responses to membrane potential. The VSD can also be regulated by interaction with intracellular proteins and ligands, but how this occurs is poorly understood. Here, we show that the VSD of the BK-type K(+) channel ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0705873104

    authors： Yang H,Hu L,Shi J,Delaloye K,Horrigan FT,Cui J

    更新日期：2007-11-13 00:00:00

  • Regulation of the galactose pathway in Saccharomyces cerevisiae: induction of uridyl transferase mRNA and dependency on GAL4 gene function.

    abstract：:In Saccharomyces cerevisiae, utilization of galactose requires four inducible enzyme activities. Three of these activities (galactose-1-phosphate uridyl transferase, EC 2.7.7.10; uridine diphosphogalactose 4-epimerase, EC 5.1.3.2; and galactokinase, EC 2.7.1.6) are specified by three tightly linked genes (GAL7, GAL10,...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.6.2878

    authors： Hopper JE,Broach JR,Rowe LB

    更新日期：1978-06-01 00:00:00

  • Essential roles of the bHLH transcription factor Hrt2 in repression of atrial gene expression and maintenance of postnatal cardiac function.

    abstract：:The basic helix-loop-helix transcriptional repressor Hairy-related transcription factor 2 (Hrt2) is expressed in ventricular, but not atrial, cardiomyocytes, and in endothelial and vascular smooth muscle cells. Mice homozygous for a null mutation of Hrt2 die perinatally from a spectrum of cardiac abnormalities, raisin...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0702447104

    authors： Xin M,Small EM,van Rooij E,Qi X,Richardson JA,Srivastava D,Nakagawa O,Olson EN

    更新日期：2007-05-08 00:00:00