Abstract:
:Skin wound infections are a significant health problem, and antibiotic resistance is on the rise. Mast cells (MCs) have been shown to contribute to host-defense responses in certain bacterial infections, but their role in skin wound superinfection is unknown. We subjected 2 MC-deficient mouse strains to Pseudomonas aeruginosa skin wound infection and found significantly delayed wound closure in infected skin wounds. This delay was associated with impaired bacterial clearance in the absence of MCs. Engraftment of MCs restored both bacterial clearance and wound closure. Bacterial killing was dependent on IL-6 released from MCs, and engraftment with IL-6-deficient MCs failed to control wound infection. Treatment with recombinant IL-6 enhanced bacterial killing and resulted in the control of wound infection and normal wound healing in vivo. Taken together, our results demonstrate a defense mechanism for boosting host innate immune responses, namely effects of MC-derived IL-6 on antimicrobial functions of keratinocytes.
journal_name
Proc Natl Acad Sci U S Aauthors
Zimmermann C,Troeltzsch D,Giménez-Rivera VA,Galli SJ,Metz M,Maurer M,Siebenhaar Fdoi
10.1073/pnas.1908816116subject
Has Abstractpub_date
2019-10-08 00:00:00pages
20500-20504issue
41eissn
0027-8424issn
1091-6490pii
1908816116journal_volume
116pub_type
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