Abstract:
:Poly(A)-specific ribonuclease (PARN), a multifunctional multi-domain deadenylase, is crucial to the regulation of mRNA turnover and the maturation of various non-coding RNAs. Despite extensive studies of the well-folding domains responsible for PARN catalysis, the structure and function of the C-terminal domain (CTD) remains elusive. PARN is a cytoplasm-nucleus shuttle protein with concentrated nucleolar distribution. Here, we identify the nuclear and nucleolar localization signals in the CTD of PARN. Spectroscopic studies indicated that PARN-CTD is intrinsically disordered with loosely packed local structures/tertiary structure. Phosphorylation-mimic mutation S557D disrupted the local structure and facilitated the binding of the CTD with the well-folded domains, with no impact on PARN deadenylase activity. Under normal conditions, the nucleolus-residing PARN recruited CBP80 into the nucleoli to repress its deadenylase activity, while DNA damage-induced phosphorylation of PARN-S557 expelled CBP80 from the nucleoli to discharge activity inhibition and attracted nucleoplasm-located CstF-50 into the nucleoli to activate deadenylation. The structure switch-induced function switch of PARN reshaped the profile of small nuclear non-coding RNAs to respond to DNA damage. Our findings highlight that the structure switch of the CTD induced by posttranslational modifications redefines the subset of binding partners, and thereby the RNA targets in the nucleoli.
journal_name
Cellsjournal_title
Cellsauthors
Duan TL,He GJ,Hu LD,Yan YBdoi
10.3390/cells8080836subject
Has Abstractpub_date
2019-08-05 00:00:00issue
8issn
2073-4409pii
cells8080836journal_volume
8pub_type
杂志文章相关文献
Cells文献大全abstract::Cell division cycle 42 (CDC42), a small Rho GTPase, plays a critical role in many cellular processes, including cell proliferation and survival. CDC42 interacts with the CRIB (Cdc42- and Rac-interactive binding) domain of CDC42SE1, a small effector protein of 9 kDa. We found that the expression of CDC42SE1 was reduced...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells8020117
更新日期:2019-02-02 00:00:00
abstract::Nonalcoholic fatty liver disease (NAFLD) is a leading cause of chronic liver diseases with an increasing prevalence due to rising rates of obesity, metabolic syndrome and type II diabetes. Untreated NAFLD may progress to steatohepatitis (NASH) and ultimately liver cirrhosis. NAFLD is characterized by lipid accumulatio...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9061346
更新日期:2020-05-28 00:00:00
abstract::Hepatic fibrosis is the consequence of an unresolved wound healing process in response to chronic liver injury and involves multiple cell types and molecular mechanisms. The hepatic endocannabinoid and apelin systems are two signalling pathways with a substantial role in the liver fibrosis pathophysiology-both are upr...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells8111311
更新日期:2019-10-24 00:00:00
abstract::Respiratory syncytial virus (RSV) is a key cause of severe respiratory infection in infants, immunosuppressed adults, and the elderly worldwide, but there is no licensed vaccine or effective, widely-available antiviral therapeutic. We recently reported staged redistribution of host cell mitochondria in RSV infected ce...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells8111417
更新日期:2019-11-11 00:00:00
abstract::Circular RNAs (circRNAs) belong to the ever-growing class of naturally occurring noncoding RNAs (ncRNAs) molecules. Unlike linear RNA, circRNAs are covalently closed transcripts mostly generated from precursor-mRNA by a non-canonical event called back-splicing. They are highly stable, evolutionarily conserved, and wid...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9030659
更新日期:2020-03-09 00:00:00
abstract::The mechanism of intercellular transmission of pathological agents in neurodegenerative diseases has received much recent attention. Huntington's disease (HD) is caused by a monogenic mutation in the gene encoding Huntingtin (HTT). Mutant HTT (mHTT) harbors a CAG repeat extension which encodes an abnormally long polyg...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells7060059
更新日期:2018-06-14 00:00:00
abstract::The Drosophila ovary offers a suitable model system to study the mechanisms that orchestrate diverse cellular processes. Oogenesis starts from asymmetric stem cell division, proper differentiation and the production of fully patterned oocytes equipped with all the maternal information required for embryogenesis. Spati...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9020274
更新日期:2020-01-22 00:00:00
abstract::Although del Río-Hortega originally reported that leptomeningeal cells are the source of ramified microglia in the developing brain, recent views do not seem to pay much attention to this notion. In this study, in vitro experiments were conducted to determine whether leptomeninges generate ramified microglia. The lept...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells10010024
更新日期:2020-12-25 00:00:00
abstract::The activation of the nuclear factor-κB (NF-κB) pathway plays a central role in the initiation and progression of inflammation, which contributes to the pathogenesis and progression of various human diseases including kidney, brain, and other diseases. Tissue plasminogen activator (tPA), a serine protease regulating h...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9061348
更新日期:2020-05-29 00:00:00
abstract::Fungal keratitis is a potentially blinding infection of the cornea that afflicts diverse patient populations worldwide. The development of better treatment options requires a more thorough understanding of both microbial and host determinants of pathology, and a spectrum of experimental models have been developed towa...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9071713
更新日期:2020-07-16 00:00:00
abstract::Insulin and insulin-like growth factor-1 (IGF1) have important roles in breast cancer development. The recent identification of nuclear insulin (INSR) and IGF1 (IGF1R) receptors provides a novel paradigm in the area of signal transduction. The fact that INSR and IGF1R can function as transcription factors, capable of ...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells8121499
更新日期:2019-11-23 00:00:00
abstract::Vector transmission of plant viruses is basically of two types that depend on the virus helper component proteins or the capsid proteins. A number of plant viruses belonging to disparate groups have developed unusual capsid proteins providing for interactions with the vector. Thus, cauliflower mosaic virus, a plant pa...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells10010090
更新日期:2021-01-07 00:00:00
abstract::Tissue inhibitor of metalloproteinase-3 (TIMP-3) is a component of the extracellular environment, where it mediates diverse processes including matrix regulation/turnover, inflammation and angiogenesis. Rare TIMP-3 risk alleles and mutations are directly linked with retinopathies such as age-related macular degenerati...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9010039
更新日期:2019-12-21 00:00:00
abstract::Membrane-bound proteases play a key role in biology by degrading matrix proteins or shedding adhesion receptors. MT1-MMP metalloproteinase is critical during cancer invasion, angiogenesis, and development. MT1-MMP activity is strictly regulated by internalization, recycling, autoprocessing but also through its incorpo...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9020348
更新日期:2020-02-03 00:00:00
abstract::While high levels of saturated fatty acids are associated with impairment of cardiovascular functions, n-3 polyunsaturated fatty acids (PUFAs) have been shown to exert protective effects. However the molecular mechanisms underlying this evidence are not completely understood. In the present study we have used rat H9c2...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9030537
更新日期:2020-02-26 00:00:00
abstract::Glycogen synthase kinase 3β (GSK3β), originally described as a negative regulator of glycogen synthesis, is a molecular hub linking numerous signaling pathways in a cell. Specific GSK3β inhibitors have anti-depressant effects and reduce depressive-like behavior in animal models of depression. Therefore, GSK3β is sugge...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9030727
更新日期:2020-03-16 00:00:00
abstract::Cannabidiol (CBD), as the only phytocannabinoid that has no psychoactive effect, has both antioxidant and anti-inflammatory effects, and thus might be suggested as a cytoprotective compound against UV-induced metabolic changes in skin cells. Therefore, the aim of this study was to investigate the level of protective C...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells8090995
更新日期:2019-08-28 00:00:00
abstract::Host immune responses play an important role in the outcome of infection with hepatitis C virus (HCV). They can lead to viral clearance and a positive outcome, or progression and severity of chronic disease. Extensive research in the past >25 years into understanding the immune responses against HCV have still resulte...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells8050507
更新日期:2019-05-26 00:00:00
abstract::Respiratory syncytial virus (RSV) infection affects the lives of neonates throughout the globe, causing a high rate of mortality upon hospital admission. Yet, therapeutic options to deal with this pulmonary pathogen are currently limited. Helminth therapy has been well received for its immunomodulatory role in hosts, ...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9051314
更新日期:2020-05-25 00:00:00
abstract::Autophagy is a key cellular process that involves constituent degradation and recycling during cellular development and homeostasis. Autophagy also plays key roles in antimicrobial host defense and numerous pathogenic organisms have developed strategies to take advantage of and/or modulate cellular autophagy. Several ...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9040805
更新日期:2020-03-26 00:00:00
abstract::MicroRNAs (miRNAs), a class of small non-coding RNA molecules, are responsible for RNA silencing and post-transcriptional regulation of gene expression. They can mediate a fine-tuned crosstalk among coding and non-coding RNA molecules sharing miRNA response elements (MREs). In a suitable environment, both coding and n...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9071574
更新日期:2020-06-28 00:00:00
abstract::Second-generation antipsychotics (SGAs) are the cornerstone of treatment for schizophrenia because of their high clinical efficacy. However, SGA treatment is associated with severe metabolic alterations and body weight gain, which can increase the risk of type 2 diabetes and cardiovascular disease, and greatly acceler...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells8111336
更新日期:2019-10-29 00:00:00
abstract::During embryonic development, the heart undergoes complex morphogenesis from a liner tube into the four chambers consisting of ventricles, atria and valves. At the same time, the cardiomyocytes compact into a dense, aligned, and highly vascularized myocardium. The extracellular matrix (ECM) is known to play an importa...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9020285
更新日期:2020-01-24 00:00:00
abstract::The γ-aminobutyric acid type A receptor-associated protein (GABARAP) and its close paralogs GABARAPL1 and GABARAPL2 constitute a subfamily of the autophagy-related 8 (Atg8) protein family. Being associated with a variety of dynamic membranous structures of autophagic and non-autophagic origin, Atg8 proteins functional...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9051296
更新日期:2020-05-22 00:00:00
abstract::Cyclic AMP promotes EPAC1 and EPAC2 activation through direct binding to a specific cyclic nucleotide-binding domain (CNBD) within each protein, leading to activation of Rap GTPases, which control multiple cell responses, including cell proliferation, adhesion, morphology, exocytosis, and gene expression. As a result,...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells8111425
更新日期:2019-11-12 00:00:00
abstract::Innate lymphoid cells (ILCs) are tissue-resident sentinels of the immune system that function to protect local tissue microenvironments against pathogens and maintain homeostasis. However, because ILCs are sensitively tuned to perturbations within tissues, they can also contribute to host pathology when critical activ...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells9020334
更新日期:2020-02-01 00:00:00
abstract::In contrast to the programmed nature of development, it is still a matter of debate whether aging is an adaptive and regulated process, or merely a consequence arising from a stochastic accumulation of harmful events that culminate in a global state of reduced fitness, risk for disease acquisition, and death. Similarl...
journal_title:Cells
pub_type: 杂志文章,评审
doi:10.3390/cells8111446
更新日期:2019-11-15 00:00:00
abstract::In liver transplantation (LT), organ shortage has led to the use of steatotic and non-steatotic grafts from donors after cardiocirculatory death (DCD). However, these grafts, especially those with steatosis, exhibit poor post-operative outcomes. To address this problem, we investigated the roles of gut-derived glucago...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells8121599
更新日期:2019-12-09 00:00:00
abstract::More than 50% of human cancers harbor TP53 mutations and increased expression of Mouse double minute 2 homolog(MDM2), which contribute to cancer progression and drug resistance. Renal cell carcinoma (RCC) has an unusually high incidence of wild-type p53, with a mutation rate of less than 4%. MDM2 is master regulator o...
journal_title:Cells
pub_type: 杂志文章
doi:10.3390/cells9061475
更新日期:2020-06-16 00:00:00
abstract::Macroautophagy (hereafter referred to as autophagy, a word derived from Greek meaning "auto-digestion") is a lysosome-dependent quality control process to degrade and turnover damaged or senescent organelles and proteins for cellular renewal [...]. ...
journal_title:Cells
pub_type: 社论
doi:10.3390/cells8070743
更新日期:2019-07-19 00:00:00