Abstract:
:Diabetes mellitus (DM) is a potential etiology of disc degeneration. Glucagon-like peptide-1 (GLP-1) is currently regarded as a powerful treatment option for type 2 diabetes. Apart from the beneficial effects on glycaemic control, GLP-1 has been reported to exert functions in a variety of tissues on modulation of cell proliferation, differentiation, and apoptosis. However, little is known regarding the effects of GLP-1 on nucleus pulposus cells (NPCs). In the present study, we investigated the effects of liraglutide (LIR), a long-lasting GLP-1 analogue, on apoptosis of human NPCs and the underlying mechanisms involved. We confirmed the presence of GLP-1 receptor (GLP-1R) in NPCs. Our data demonstrated that liraglutide inhibited the apoptosis of NPCs induced by high glucose (HG), as detected by Annexin V/Propidium Iodide (PI) and ELISA assays. Moreover, liraglutide down-regulated caspase-3 activity at intermediate concentration (100 nM) for maximum effect. Further analysis suggested that liraglutide suppressed reactive oxygen species (ROS) generation and stimulated the phosphorylation of Akt under HG condition. Pretreatment of cells with the Phosphoinositide 3-kinase (PI3K) inhibitor LY294002 (LY) and small interfering RNAs (siRNAs) GLP-1R abrogated the liraglutide-induced activation of Akt and the protective effects on NPCs' apoptosis. In conclusion, liraglutide could directly protect NPCs against HG-induced apoptosis by inhibiting oxidative stress and activate the PI3K/Akt/caspase-3 signaling pathway via GLP-1R.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Ming-Yan Y,Jing Z,Shu-Qin G,Xiao-Liang B,Zhi-Hong L,Xue Zdoi
10.1042/BSR20190109subject
Has Abstractpub_date
2019-08-19 00:00:00issue
8eissn
0144-8463issn
1573-4935pii
BSR20190109journal_volume
39pub_type
杂志文章abstract::Extracts from uterine cervical and body cancers, but not from benign tumor or intact tissues tested, were found to contain a growth-promoting activity which induced the proliferation of human endometrial fibroblasts. Exposure of cultured fibroblasts to the cancer extracts increased the rate of [3H]thymidine incorporat...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116850
更新日期:1990-02-01 00:00:00
abstract::This study is part of an attempt to understand the role of specific cellular activities in the bone resorptive process. Experiments were performed whereby known pharmacological agents were used to inhibit individual modes of osteoclastic activity, such as motility and secretion. The effects of such treatments on bone ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116615
更新日期:1990-12-01 00:00:00
abstract::Matrix-bound monoclonal antibodies against urokinase have been used to purify this enzyme by affinity chromatography. In a single-step procedure, urokinase can be isolated from crude preparations with high yield and high purity, and without loss of enzymatic activity. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122902
更新日期:1983-04-01 00:00:00
abstract::Long noncoding RNA (lncRNA) differentiation antagonizing nonprotein coding RNA (DANCR) plays important regulatory roles in many solid tumors. However, the effect of DANCR in glioma progression and underlying molecular mechanisms were not entirely explored. In the present study, we determined the expression of DANCR in...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20171664
更新日期:2018-02-02 00:00:00
abstract::The cellular onc-genes c-src and c-yes are expressed very differently during chicken embryonic development. The c-src mRNA and its translational product are detectable at high levels in brain extracts of chicken embryos and adult chickens, whereas muscle extracts show an age-dependent decrease in the amounts of c-src-...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01128817
更新日期:1984-09-01 00:00:00
abstract::In the course of evolution, mitochondria lost their independence, and mtDNA became "slave" of nDNA, depending on numerous nucleus-encoded factors for its integrity, replication and expression. Mutations in any of these factors may alter the cross-talk between the two genomes and cause diseases that affect mtDNA integr...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/s10540-007-9036-1
更新日期:2007-06-01 00:00:00
abstract::Cryptococcus neoformans is an opportunistic fungal pathogen that causes life-threatening meningitis primarily in immunocompromised individuals. In order to survive and proliferate during infection, C. neoformans must adapt to a variety of stresses it encounters within the host. Patient outcome depends on the interacti...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190337
更新日期:2020-10-30 00:00:00
abstract::Neuroblastoma is the most common tumor in children, with a very poor prognosis. It is urgent to identify novel biomarkers to treat neuroblastoma, together with surgery, chemotherapy, and radiation. Human tripartite motif 59 (TRIM59), a member of the TRIM family, has been reported to participate in several human tumors...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181277
更新日期:2019-01-18 00:00:00
abstract::Disc nucleus pulposus (NP) cell experiences periodic osmolarity alterations during daily activities, which has been proved to affect cell biology in vitro The present study was aimed to investigate the effects of cartilage-derived morphogenetic protein-2 (CDMP-2) on NP cell apoptosis under the hyperosmolarity culture ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181176
更新日期:2018-10-09 00:00:00
abstract::Corneal inflammation or keratitis is a significant cause of ocular morbidity around the world. Fortunately, the majority of the cases are successfully managed with medical therapy, but the failure of therapy does occur, leading to devastating consequences of either losing the vision or the eye. This review attempts to...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1023/a:1017939725776
更新日期:2001-08-01 00:00:00
abstract::The first demonstration of a method to incorporate radiolabeled methyl groups from [14C]formaldehyde into membrane proteins in human erythrocytes by reductive methylation in the presence of NaCNBH3 is described. This method is based on the work of Jentoft and Dearborn [(1979) J. Biol. Chem. 254, 4359-4365], who demons...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01136722
更新日期:1982-04-01 00:00:00
abstract::Artocarpus incisa (breadfruit) seeds contain three different lectins (Frutalin, Frutapin (FTP) and Frutackin) with distinct carbohydrate specificities. The most abundant lectin is Frutalin, an α-D-galactose-specific carbohydrate-binding glycoprotein with antitumour properties and potential for tumour biomarker discove...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20170969
更新日期:2017-07-21 00:00:00
abstract::Background: Nasopharyngeal carcinoma (NPC) is a disease highly sensitive to radiotherapy with the unclear etiology. However, the specific effects of microRNA-613 (miR-613) on NPC still remain elusive. Therefore, the present study probes into the underlying mechanism of miR-613 in NPC via AKT signaling pathway by regul...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20182196
更新日期:2019-07-10 00:00:00
abstract::Nonsmall cell lung cancer (NSCLC) is one of the most frequent malignancies worldwide. Long noncoding RNAs (LncRNAs) play critical roles in cancer initiation and progression. Previous studies have demonstrated that overexpression of cytoskeleton regulator RNA (CYTOR) predicates poor prognosis and promotes tumor progres...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181599
更新日期:2018-12-18 00:00:00
abstract::Cathepsin D occurs in two forms, a single polypeptide chain (Mr 44 000) and a non-covalent complex of two peptides of Mr 14 000 and 30 000 that is derived by proteolytic processing of the 44 000 polypeptide. The two forms from bovine spleen are closely similar in secondary structure content, in aromatic amino acid env...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119908
更新日期:1985-10-01 00:00:00
abstract::Mice and rats are animals commonly used in research and laboratory testing. Compared with other animal species, they harbor many more zoonotic agents. Hymenolepis nana (H. nana) is a common tapeworm that parasitizes both humans and rodents. Although this tapeworm is of socio-economic importance worldwide, information ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181224
更新日期:2019-02-08 00:00:00
abstract::A novel approach was used to assess the role of phosphoinositide hydrolysis in the mitogenic action of phytohemagglutinin (PHA) or concanavalin A (ConA). The treatment of human peripheral blood leukocytes (PBL) with monospecific antibodies against phospholipase C (PLC) produced a dose-dependent inhibition (up to 100%)...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116866
更新日期:1987-09-01 00:00:00
abstract::Immobilization of yeast mitochondrial RNA on nitrocellulose filters without prior alkali treatment revealed a low-MW RNA species (integral of 300 bases) which hybridizes specifically to the RNA coding strand of a DNA fragment BglII-HinfI at the 3' end of the COB-BOX gene. This RNA species (often a doublet) was found i...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116301
更新日期:1981-07-01 00:00:00
abstract::Micro-inflammation plays an important role in the pathogenesis of spontaneously hypertensive rat (SHR). In the present study, we investigated the therapeutic potential of resveratrol (RSV), a polyphenol with anti-fibrosis activity in hypertensive renal damage model. In SHR renal damage model, RSV treatment blunted the...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20160035
更新日期:2016-06-03 00:00:00
abstract::CXCL8, a member of CXC chemokines, was constitutively expressed in many types of human cancers, and its overexpression has been shown to play a critical role in promoting tumorigenesis. The purpose of the present study was to determine CXCL8 expression in a commercial human liver tissue microarray, and elucidate the e...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20201169
更新日期:2020-08-28 00:00:00
abstract::A non-ionic detergent such as Lubrol-PX extracts in soluble form the VIP-binding structures of rat liver plasma membranes. Detergent-solubilized proteins bind specifically [125I]VIP and the complex tracer-protein is identified by the use of Sepharose 6B columns. The interaction is only possible in the absence of deter...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01145177
更新日期:1986-01-01 00:00:00
abstract::The sarcolemma of smooth-muscle cells from human pregnant myometrium possesses a high-affinity Ca2+-ATPase, which has the characteristics of an active Ca2+-extrusion pump. This pump enzyme, either membrane-bound or solubilized, was strongly inhibited by oxytocin (half-maximal inhibition at about 4 microU/ml or 10 pM)....
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117437
更新日期:1985-01-01 00:00:00
abstract::Ursodeoxycholic acid (UDCA) and its taurine-conjugate, tauroursodeoxycholic acid (TUDCA), play a unique role in modulating the apoptotic threshold in cells. The mechanism is thought to involve, in part, inhibition of translocation for Bax from the cytosol to mitochondria. Here, we attempted to use the native fluoresce...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/s10540-006-9019-7
更新日期:2006-06-01 00:00:00
abstract::Bladder cancer is a common malignant tumour worldwide. Epithelial-mesenchymal transition (EMT)-related biomarkers can be used for early diagnosis and prognosis of cancer patients. To explore, accurate prediction models are essential to the diagnosis and treatment for bladder cancer. In the present study, an EMT-relate...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20203944
更新日期:2021-01-29 00:00:00
abstract::The coupling of the chemical reaction of ATP hydrolysis to the transport of calcium from the cytoplasm into the lumen of sarcoplasmic reticulum vesicles can be defined by a set of rules that define alternating changes in the specificities of the enzyme for catalysis of chemical and physical reactions. ...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/BF01788360
更新日期:1995-10-01 00:00:00
abstract:BACKGROUND:Emerging evidences have indicated that long non-coding RNAs (LncRNAs) play vital roles in cancer development and progression. Previous studies have suggested that overexpression of SPRY4 intronic transcript 1 (SPRY4-IT1) predicates poor prognosis and promotes tumor progress in cervical cancer (CC). However, ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181339
更新日期:2019-06-04 00:00:00
abstract::Pulmonary arterial hypertension (PAH) is a life-threatening chronic cardiopulmonary disorder. However, studies providing PAH-related gene expression profiles are scarce. To identify hub genes involved in PAH, we investigate two microarray data sets from gene expression omnibus (GEO). A total of 150 differentially expr...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20202346
更新日期:2020-09-30 00:00:00
abstract::MicroRNA-326 (miR-326) was reported to be dysregulated and involved in the progression of multiple cancers. However, the clinical significance, biological role and underlying mechanism of miR-326 in the carcinogenesis of breast cancer are still unclear. In the present study, we showed that miR-326 was down-regulated i...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190787
更新日期:2019-07-29 00:00:00
abstract::Hematopoietic pre-B cell leukemia transcription factor (PBX)-interacting protein (RRBP1) has been shown to participate in various aspects of malignancies. The clinical significance of RRBP1 and its involvement in the epithelial ovarian cancer have yet to be studied. The aim of the present study was to investigate the ...
journal_title:Bioscience reports
pub_type: 临床试验,杂志文章
doi:10.1042/BSR20190656
更新日期:2019-07-23 00:00:00
abstract::This review covers the recent progress in the elucidation of the structure of photosystem II (PSII). Because much of the structural information for this membrane protein complex has been revealed by electron microscopy (EM), the review will also consider the specific technical and interpretation problems that arise wi...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/BF01206204
更新日期:1996-04-01 00:00:00