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VCP Machinery Mediates Autophagic Degradation of Empty Argonaute.

Abstract：

:The Argonaute subfamily of proteins (AGO) loads microRNAs (miRNAs) to form the effector complex that mediates target gene silencing. Empty AGO, but not miRNA-loaded AGO, is selectively degraded across species. We have reported that the degradation of empty AGO is part of a quality control pathway that eliminates dysfunctional AGO. However, how empty AGO is degraded remains unclear. Here we show that the empty state of Drosophila Ago1 is degraded by autophagy. Comprehensive LC-MS/MS analyses, together with manipulation of the Ago1 ubiquitination level, revealed that VCP, which mediates selective autophagy, recognizes empty Ago1 via the Ufd1-Npl4 heterodimer. Depletion of VCP-Ufd1-Npl4 machinery impairs degradation of empty Ago1 and miRNA-mediated target gene silencing. Our findings reveal a direct link between empty AGO degradation and selective autophagy that ensures efficient miRNA function.

journal_name

Cell Rep

journal_title

Cell reports

authors

Kobayashi H,Shoji K,Kiyokawa K,Negishi L,Tomari Y

doi

10.1016/j.celrep.2019.07.003

subject

Has Abstract

pub_date

2019-07-30 00:00:00

pages

1144-1153.e4

issue

5

issn

2211-1247

pii

S2211-1247(19)30887-3

journal_volume

28

pub_type

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