Abstract:
:Herein, we explored the interaction of Al2O3 NPs with RBCs and Hb to determine the effect of Al2O3 NPs on hemolytic activity and Hb denaturation. The percentage of hemolysis of extracts and direct contact assays triggered by Al2O3 NPs was calculated by determining supernatant Hb concentration at 540 nm. Far-UV CD and Trp/ANS/acrylamide fluorescence spectroscopic methods were used to determine the structural changes of Hb upon interaction with Al2O3 NPs. Theoretical studies were carried out to display the residues involved in the binding site of Hb with Al2O3 nanocluster as well as the structural changes of Hb after interaction. The results showed that the percentage of hemolysis of extract and direct contact assays induced by Al2O3 NPs were 1.16 and 0.46, respectively. Fluorescence spectroscopy revealed that Al2O3 NPs alter the quaternary structure of the protein; however, CD spectroscopy indicated that the secondary structure of Hb remains almost unchanged. Theoretical study displayed that Al2O3 nanocluster interacts with different residues of protein, and Hb tends to be destabilized at the binding site with nanocluster. This study may be significant in exploring the toxicity profile of Al2O3 NPs for their in vivo implementations.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Kahbasi S,Samadbin M,Attar F,Heshmati M,Danaei D,Rasti B,Salihi A,Nanakali NMQ,Aziz FM,Akhtari K,Hasan A,Falahati Mdoi
10.1016/j.ijbiomac.2019.07.154subject
Has Abstractpub_date
2019-10-01 00:00:00pages
800-809eissn
0141-8130issn
1879-0003pii
S0141-8130(19)34465-4journal_volume
138pub_type
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