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Endometrial microbiota in infertile women with and without chronic endometritis as diagnosed using a quantitative and reference range-based method.

Abstract：

OBJECTIVE:To systematically compare the endometrial microbiota in infertile women with and without chronic endometritis (CE), as diagnosed by a quantitative and reference range-based method. DESIGN:Case-control observational study. SETTING:University-affiliated hospital. PATIENT(S):One hundred and thirty infertile women. INTERVENTION(S):Endometrial biopsy and fluid (uterine lavage, UL) collected precisely 7 days after LH surge, with plasma cell density (PCD) determined based on Syndecan-1 (CD138)-positive cells in the entire biopsy section and culture-independent massively parallel sequencing of the 16S ribosomal RNA gene performed on both the CE and non-CE endometrial fluid samples. MAIN OUTCOME MEASURE(S):Relative abundance of bacterial taxa. RESULT(S):Chronic endometritis was diagnosed if the PCD was above the 95th percentile (>5.15 cells per 10 mm2) of the reference range in fertile control subjects. With this stringent diagnostic criterion, 12 women (9%) were diagnosed with CE. Sequencing was successfully performed on all endometrial samples obtained by UL) (CE, n = 12; non-CE, n = 118). The median relative abundance of Lactobacillus was 1.89% and 80.7% in the CE and non-CE microbiotas, respectively. Lactobacillus crispatus was less abundant in the CE microbiota (fold-change, range: 2.10-2.30). Eighteen non-Lactobacillus taxa including Dialister, Bifidobacterium, Prevotella, Gardnerella, and Anaerococcus were more abundant in the CE microbiota (fold-change, 2.10-18.9). Of these, Anaerococcus and Gardnerella were negatively correlated in relative abundance with Lactobacillus (SparCC correlation magnitude, range: 0.142-0.177). CONCLUSION(S):Chronic endometritis was associated with a statistically significantly higher abundance of 18 bacterial taxa in the endometrial cavity. CLINICAL TRIALS REGISTRY NUMBER:ChiCTR-IOC-16007882.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Liu Y,Ko EY,Wong KK,Chen X,Cheung WC,Law TS,Chung JP,Tsui SK,Li TC,Chim SS

doi

10.1016/j.fertnstert.2019.05.015

subject

Has Abstract

pub_date

2019-10-01 00:00:00

pages

707-717.e1

issue

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(19)30471-6

journal_volume

112

pub_type

杂志文章

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Endometrial microbiota in infertile women with and without chronic endometritis as diagnosed using a quantitative and reference range-based method.