Abstract:
:Freezing and storage of human male gametes is associated with a reduction in the overall semen quality and establishment of pregnancy. This study was done to evaluate the integrity of sperm head ultrastructure (SHU) with computerized and vapor freezing. Comparisons were made between the effect of cryopreservatives glycerol (G) and dimethylsulfoxide (DMSO) on SHU. Twelve ejaculates from five proven fertile donors were studied with the use of routine semen analysis, zona-free hamster ova, and SHU. Both cooling processes, regardless of the preservative used, significantly reduced sperm function and the number of SH with intact plasma membranes. The staged cooling technique was substantially superior to vapor freezing in all parameters analyzed (P less than 0.01). G was less detrimental to the postthaw SHU than 1 M DMSO. A significant positive correlation (r = 0.98; P less than 0.01) was noted between the total number of intact SH and motile sperm. Computerized freezing in a G-diluted semen rendered a sperm environment that allowed the highest number of forms with intact SH membranes and with the best chances to penetrate zona-free hamster ova.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Serafini PC,Hauser D,Moyer D,Marrs RPdoi
10.1016/s0015-0282(16)49650-0subject
Has Abstractpub_date
1986-10-01 00:00:00pages
691-5issue
4eissn
0015-0282issn
1556-5653pii
S0015-0282(16)49650-0journal_volume
46pub_type
杂志文章abstract::Chronic and transient hyperprolactinemia have been associated with luteal phase dysfunction in both spontaneously cycling women and those subjected to ovarian hyperstimulation by exogenous gonadotropins. It is the objective of the in vitro and in vivo studies reported herein to examine the possibility that prolactin (...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1987-07-01 00:00:00
abstract:OBJECTIVE:To determine if short-term weight change among women with unexplained infertility (UI) and polycystic ovary syndrome (PCOS) undergoing ovulation induction is associated with live birth. DESIGN:Secondary analysis of randomized trials. SETTING:Multicenter fertility trial sites. PATIENT(S):A total of 900 wome...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2020.06.002
更新日期:2020-11-01 00:00:00
abstract::Heterologous antibodies have frequently been used to develop assays in other species. Human urinary LH kits, widely available for predicting the LH surge, offer a unique opportunity to determine the timing of ovulation and mating in nonhuman primates. We tested three commercially available ovulation predictor kits for...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)53301-9
更新日期:1990-02-01 00:00:00
abstract::Immunocytochemical, biochemical, and histologic analysis of endometriotic lesions and endometria from rhesus macaques with endometriosis revealed several distinctions between ectopic and eutopic endometrium. In lesions, unlike endometrium, neither the mean percentages of estrogen receptor positive (ER+) cells nor the ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1988-02-01 00:00:00
abstract:OBJECTIVE:To evaluate the safety profile of endometrial ablation performed with a thermal balloon as defined by serosal temperature elevation and depth of injury. DESIGN:Observational study with histopathologic correlation conducted in ex vivo and in vivo phases. SETTING:Academic medical center. PATIENT(S):Twenty pa...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(98)00245-3
更新日期:1998-10-01 00:00:00
abstract:OBJECTIVE:To examine the effect of high doses of gonadotropins on IVF outcome in normal (intermediate) responders. DESIGN:Retrospective analysis of patients undergoing IVF therapy from 1990 to 1994 at our institution. SETTING:Academic tertiary center. PATIENTS:A homogeneous group of women that consisted of intermedi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)57621-3
更新日期:1995-06-01 00:00:00
abstract::Eight premenopausal women with cancer had blood drawn for analysis of brain-derived neurotrophic factor (BDNF) and müllerian-inhibiting substance (MIS) before and 3 months after receiving chemotherapy. Unlike MIS, BDNF levels were not reduced after chemotherapy. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.10.033
更新日期:2011-04-01 00:00:00
abstract:OBJECTIVE:To document the changes in pain scores 3-12 months following ablative laparoscopic surgery. Secondary outcome measures included patient satisfaction scores. DESIGN:A prospective, cohort study. SETTING:A tertiary referral center for the treatment of endometriosis. PATIENT(S):Seventy-three consecutive women ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(02)04957-9
更新日期:2003-05-01 00:00:00
abstract:OBJECTIVE:To determine the hormonal characteristics of a fecund menstrual cycle. DESIGN:Prospective observational study. SETTING:Clinical research center. PATIENT(S):Fourteen patients having artificial insemination with donor semen provided daily blood and urine samples in a nonconceptive cycle and the consecutive c...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(00)01765-9
更新日期:2001-03-01 00:00:00
abstract::Most oocyte donation programs have experienced higher pregnancy rates than usually seen in regular in vitro fertilization (IVF), suggesting that the quality of either the oocytes or the endometrium is superior. To clarify this issue we analyzed the results of transfers of 136 cryopreserved embryos originating either f...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)53830-8
更新日期:1990-10-01 00:00:00
abstract:OBJECTIVE:To evaluate the effects of a pill with drospirenone (3 mg) plus ethinyl E(2) (20 μg) (DRP/20EE) alone or associated with metformin or cyproterone acetate (CPA) on some metabolic cardiovascular risk factors in women with polycystic ovary syndrome (PCOS). DESIGN:Randomized, open-label clinical trial. SETTING:...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2009.10.016
更新日期:2010-10-01 00:00:00
abstract::Pulsatile administration of human menopausal gonadotropin (hMG) via the subcutaneous route was evaluated in 15 patients with various ovulatory disorders. Administration of hMG was started at a dose of 4.6875 IU (75 IU/day) or 9.375 IU (150 IU/day) per pulse every 90 minutes. Ovulation was observed in 26 (92.9%) of 28 ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)49456-2
更新日期:1986-07-01 00:00:00
abstract:OBJECTIVE:To establish an intracytoplasmic sperm injection treatment program for couples with male infertility and to determine those factors important for success. DESIGN:A retrospective analysis of 171 consecutive cycles of intracytoplasmic sperm injection concerning 145 infertile couples. SETTING:Infertility clini...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)57489-5
更新日期:1995-04-01 00:00:00
abstract:OBJECTIVE:To investigate whether interleukin-1beta (IL-1beta) and interleukin-1alpha (IL-1alpha) affect the implantation rate of patients undergoing IVF-ET. DESIGN:Follicular fluid and serum were obtained on the day of hCG administration, the day of oocyte retrieval, and the day of embryo transfer. SETTING:Cellular i...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/s0015-0282(98)00243-x
更新日期:1998-09-01 00:00:00
abstract::In the United States, approximately 650,000 women of reproductive age undergo tubal sterilization each year and some of these women later have hysterectomies. Little is known about risk factors for having hysterectomy after tubal sterilization. For examination of this issue, we analyzed data from the Collaborative Rev...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)48974-0
更新日期:1985-11-01 00:00:00
abstract:OBJECTIVE:To examine the relationship of ovulation-inducing drugs and ovarian cancer. DESIGN:Retrospective cohort study, with additional follow-up since initial report. SETTING:Five large reproductive endocrinology practices. PATIENT(S):In a retrospective cohort of 9,825 women evaluated for infertility at five clini...
journal_title:Fertility and sterility
pub_type: 杂志文章,多中心研究
doi:10.1016/j.fertnstert.2013.08.008
更新日期:2013-12-01 00:00:00
abstract:OBJECTIVE:To determine whether the use of GnRH antagonist in cycles converted from ovulation induction-IUI to IVF affects cycle outcome and pregnancy rates. DESIGN:Retrospective cohort study. SETTING:Academic research institution. PATIENT(S):One hundred eighty-two consecutive patients with ovulation induction-IUI to...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2009.01.109
更新日期:2010-02-01 00:00:00
abstract::It is the responsibility of the clinician to assess for the presence of erectile dysfunction, ejaculatory dysfunction, or diminished libido related to hypoandrogenism among men presenting with a primary complaint of infertility. Referral to a reproductive urologist or other appropriate specialist with requisite expert...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2018.07.010
更新日期:2018-10-01 00:00:00
abstract:OBJECTIVE:To study the distribution and progression of terminal hair growth in pregnant women and to determine the feasibility of a simplified scoring system for assessing hirsutism. DESIGN:Prospective follow-up observational study. SETTING:Academic hospital. PATIENT(S):A total of 115 pregnant women (discovery cohor...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2015.10.036
更新日期:2016-02-01 00:00:00
abstract::Testosterone and 19-nortestosterone derivatives were evaluated in a developmental scheme designed to identify competitive progesterone antagonists having abortifacient activity. Compounds that displayed significant binding to the rabbit uterine progesterone receptor were followed in biologic tests for progestational, ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)44003-3
更新日期:1979-05-01 00:00:00
abstract:OBJECTIVE:To assess the effect of on-demand intranasal oxytocin administration on female sexual function and activity. DESIGN:Randomized, prospective, double-blind, placebo-controlled, crossover trial with duration of 22 weeks. SETTING:Academic medical center. PATIENT(S):Thirty pre-and postmenopausal women with sexu...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2015.06.010
更新日期:2015-09-01 00:00:00
abstract:OBJECTIVE:To investigate methods of fertility preservation in younger women exposed to adjuvant chemotherapy for breast cancer. DESIGN:Systematic review of literature. SETTING:Academic Department of Medical Oncology. PATIENT(S):Premenopausal women exposed to adjuvant chemotherapy for breast cancer. MAIN OUTCOME MEA...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2009.02.055
更新日期:2010-06-01 00:00:00
abstract:OBJECTIVE:To report a rare complication of GnRH agonist therapy for intestinal endometriosis. DESIGN:Case report. SETTING:University hospital. PATIENT(S):A 45-year-old nulliparous Japanese woman with catamenial digestive symptoms. INTERVENTION(S):GnRH agonist therapy. MAIN OUTCOME MEASURE(S):Acute abdomenal crisis...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.12.082
更新日期:2007-10-01 00:00:00
abstract:OBJECTIVE:To evaluate whether the developmental potential of the blastocyst is affected by the number of trophectoderm (TE) cells biopsied in preimplantation genetic diagnosis (PGD) cycles. DESIGN:Retrospective study. SETTING:University-affiliated center. PATIENT(S):Women underwent PGD cycles of blastocyst biopsy an...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2016.01.011
更新日期:2016-05-01 00:00:00
abstract:OBJECTIVE:To investigate the relationship between endogenous serum levels of human growth hormone (hGH) and ovarian response to human menopausal gonadotropins (hMG). DESIGN:Retrospective analysis of patient response to hMG. SETTING:Center for assisted reproductive technology. PATIENTS:Eighty women who had undergone ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)55133-4
更新日期:1992-07-01 00:00:00
abstract::Human menopausal gonadotropins (hMG) and clomiphene citrate (CC), either alone or in combination, are frequently used for in vitro fertilization (IVF) in an attempt to maximize the number of oocytes recovered and the number of embryos transferred. However, direct comparison of the relative efficacy of these protocols ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)49889-4
更新日期:1986-12-01 00:00:00
abstract::We tested the hypothesis that prenatal exposure to cigarette smoke is associated with impaired fertility in adulthood. Fertility for nearly 600 couples was quantified by counting the number of noncontraception cycles required to conceive. Exposure in utero was assessed by asking couples about their parents' cigarette ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1986-09-01 00:00:00
abstract:OBJECTIVE:To develop and validate a simple and reliable single-cell analysis protocol for the preimplantation genetic diagnosis (PGD) of spinal muscular atrophy (SMA). DESIGN:Molecular tests based on specific enzymatic digestion have already been described for SMA diagnosis. We modified the amplified DNA fragments so ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2005.03.054
更新日期:2005-09-01 00:00:00
abstract:OBJECTIVE:To examine the relation between blood found on the transfer catheter after ET and the rates of embryo implantation and clinical pregnancy with the use of IVF-ET. DESIGN:Retrospective cohort study. SETTING:A tertiary care center for assisted reproductive technology. PATIENT(S):Three hundred seven couples wh...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(98)00315-x
更新日期:1998-11-01 00:00:00
abstract:OBJECTIVE:To define the hypersensitive status of PRL secretion in normoprolactinemic infertile women and determine the incidence of occult hyperprolactinemia among them. DESIGN:The potential for PRL secretion was examined in 463 women. SETTING:Outpatient clinic in a university hospital. PATIENTS:Three hundred sixty-...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)56154-8
更新日期:1993-09-01 00:00:00