Suppressing the dark side of autophagy.

abstract：:It has been a longstanding problem to identify specific and efficient pharmacological modulators of autophagy. Recently, we found that depletion of acetyl-coenzyme A (AcCoA) induced autophagic flux, while manipulations designed to increase cytosolic AcCoA efficiently inhibited autophagy. Thus, the cell permeant ester ...

journal_title：Autophagy

authors： Mariño G,Pietrocola F,Kong Y,Eisenberg T,Hill JA,Madeo F,Kroemer G

更新日期：2014-05-01 00:00:00

abstract：:Although alterations of the macroautophagy/autophagy-lysosome pathway have been observed in cancer for many years, the mechanisms underlying these changes and the importance of autophagic and lysosomal reprogramming by cancer have yet to be well identified. Our recent study demonstrates that oncogenic BRAF signaling p...

journal_title：Autophagy

authors： Li S,Song Y,Quach C,Nemecio D,Liang C

更新日期：2019-10-01 00:00:00

abstract：:We detail here a protocol using tandem-tagged mCherry-EGFP-LC3 (C-G-LC3) to quantify autophagic flux in single cells by ratiometric flow cytometry and to isolate subpopulations of cells based on their relative levels of autophagic flux. This robust and sensitive method measures autophagic flux rather than autophagosom...

journal_title：Autophagy

authors： Gump JM,Thorburn A

更新日期：2014-07-01 00:00:00

abstract：:Chronic drinking leads to myocardial contractile dysfunction where ethanol metabolism plays an essential role. Acetaldehyde, the main ethanol metabolite, mediates alcohol-induced cell injury although the underlying mechanism is still elusive. This study was designed to examine the mechanism involved in accelerated eth...

journal_title：Autophagy

authors： Guo R,Hu N,Kandadi MR,Ren J

更新日期：2012-04-01 00:00:00

abstract：:In the fungal pathogen Fusarium oxysporum, vegetative hyphal fusion triggers nuclear mitotic division in the invading hypha followed by migration of a nucleus into the receptor hypha and degradation of the resident nucleus. Here we examined the role of autophagy in fusion-induced nuclear degradation. A search of the F...

journal_title：Autophagy

authors： Corral-Ramos C,Roca MG,Di Pietro A,Roncero MI,Ruiz-Roldán C

更新日期：2015-01-01 00:00:00

abstract：:Protein synthesis and autophagy work as two opposing processes to control cell growth in response to nutrient supply. The mammalian/mechanistic target of rapamycin complex 1 (mTORC1) pathway, which acts as a master regulator to control protein synthesis, has recently been shown to inhibit autophagy by phosphorylating ...

journal_title：Autophagy

authors： Dunlop EA,Hunt DK,Acosta-Jaquez HA,Fingar DC,Tee AR

更新日期：2011-07-01 00:00:00

abstract：:Cytotoxic necrotizing factor 1 is a bacterial protein toxin from Escherichia coli that is able to activate the Rho GTPases and to hinder apoptosis and mitotic catastrophe. Upon exposure to toxin, cells undergo a complex framework of changes, including cytoskeleton remodeling and multinucleation. These cells also show ...

journal_title：Autophagy

authors： Fiorentini C,Malorni W

更新日期：2006-10-01 00:00:00

abstract：:Autophagy is a highly conserved homeostatic pathway that plays an important role in tumor development and progression by acting on cancer cells in a cell-autonomous mechanism. However, the solid tumor is not an island, but rather an ensemble performance that includes nonmalignant stromal cells, such as macrophages. A ...

journal_title：Autophagy

authors： Chen P,Cescon M,Bonaldo P

更新日期：2014-02-01 00:00:00

abstract：:Autophagy and apoptosis are 2 fundamental biological mechanisms that may cooperate or be antagonistic, although both are involved in deciding the fate of cells in physiological or pathological conditions. These 2 mechanisms coexist simultaneously in cells and share common upstream signals and stimuli. Autophagy and ap...

journal_title：Autophagy

authors： Ravegnini G,Sammarini G,Nannini M,Pantaleo MA,Biasco G,Hrelia P,Angelini S

更新日期：2017-03-04 00:00:00

abstract：:Autophagy, a catabolic pathway of lysosomal degradation, acts not only as an efficient recycle and survival mechanism during cellular stress, but also as an anti-infective machinery. The human pathogen Staphylococcus aureus (S. aureus) was originally considered solely as an extracellular bacterium, but is now recogniz...

journal_title：Autophagy

authors： Neumann Y,Bruns SA,Rohde M,Prajsnar TK,Foster SJ,Schmitz I

更新日期：2016-11-01 00:00:00

abstract：:We investigated the cell-death mechanisms induced in esophageal cancer cells in response to the chemotherapeutic drugs, 5-fluorouracil (5-FU) and cisplatin. Chemosensitive cell lines exhibited apoptosis whereas chemoresistant populations exhibited autophagy and a morphology resembling type II programmed cell death (PC...

journal_title：Autophagy

authors： O'Donovan TR,O'Sullivan GC,McKenna SL

更新日期：2011-05-01 00:00:00

abstract：:Ankylosing spondylitis (AS) is a chronic autoimmune inflammatory disease with severe inflammatory symptoms in the axial skeleton. The cause of ankylosing spondylitis is unknown. TNFAIP3, also named A20, uses ubiquitin-related functions to regulate immune activation, deficiency of which is highly related to autoimmune ...

journal_title：Autophagy

authors： Zhai Y,Lin P,Feng Z,Lu H,Han Q,Chen J,Zhang Y,He Q,Nan G,Luo X,Wang B,Feng F,Liu F,Chen Z,Zhu P

更新日期：2018-01-01 00:00:00

abstract：:Minocycline has been shown to alleviate several neurological disorders. Unexpectedly, we found that minocycline had opposite effects on glioma cells: minocycline induced nonapoptotic cell death in glioma cells. The glioma cell death was associated with the presence of autophagic vacuoles in the cytoplasm. Minocycline ...

journal_title：Autophagy

authors： Liu WT,Lin CH,Hsiao M,Gean PW

更新日期：2011-02-01 00:00:00

abstract：:Apoptosis (programmed cell death type I) and autophagy (type II) are crucial mechanisms regulating cell death and homeostasis. The Bcl-2 proto-oncogene is overexpressed in 50-70% of breast cancers, potentially leading to resistance to chemotherapy, radiation and hormone therapy-induced apoptosis. Here, we investigated...

journal_title：Autophagy

authors： Akar U,Chaves-Reyez A,Barria M,Tari A,Sanguino A,Kondo Y,Kondo S,Arun B,Lopez-Berestein G,Ozpolat B

更新日期：2008-07-01 00:00:00

abstract：:KRAS is the most frequently mutated oncogene in human neoplasia. Despite a large investment to understand the effects of KRAS mutation in cancer cells, the direct effects of the oncogenetic KRAS activation on immune cells remain elusive. Here, we report that extracellular KRASG12D is essential for pancreatic tumor-ass...

journal_title：Autophagy

authors： Dai E,Han L,Liu J,Xie Y,Kroemer G,Klionsky DJ,Zeh HJ,Kang R,Wang J,Tang D

更新日期：2020-11-01 00:00:00

abstract：:Proteome profiling and global protein-interaction approaches have significantly improved our knowledge of the protein interactomes of autophagy and other cellular stress-response pathways. New discoveries regarding protein complexes, interaction partners, interaction domains, and biological roles of players that are p...

journal_title：Autophagy

authors： Ho CJ,Samarasekera G,Rothe K,Xu J,Yang KC,Leung E,Chan M,Jiang X,Gorski SM

更新日期：2020-06-07 00:00:00

abstract：:Oxidative stress-induced granulosa cell (GCs) death represents a common reason for follicular atresia. Follicle-stimulating hormone (FSH) has been shown to prevent GCs from oxidative injury, although the underlying mechanism remains to be elucidated. Here we first report that the suppression of autophagic cell death v...

journal_title：Autophagy

authors： Shen M,Jiang Y,Guan Z,Cao Y,Li L,Liu H,Sun SC

更新日期：2017-08-03 00:00:00

abstract：:In yeast, Tom22, the central component of the TOMM (translocase of outer mitochondrial membrane) receptor complex, is responsible for the recognition and translocation of synthesized mitochondrial precursor proteins, and its protein kinase CK2-dependent phosphorylation is mandatory for TOMM complex biogenesis and prop...

journal_title：Autophagy

authors： Kravic B,Harbauer AB,Romanello V,Simeone L,Vögtle FN,Kaiser T,Straubinger M,Huraskin D,Böttcher M,Cerqua C,Martin ED,Poveda-Huertes D,Buttgereit A,Rabalski AJ,Heuss D,Rudolf R,Friedrich O,Litchfield D,Marber M,Salvi

更新日期：2018-01-01 00:00:00

abstract：:Cocaine abuse leads to neuroinflammation, which, in turn, contributes to the pathogenesis of neurodegeneration associated with advanced HIV-1 infection. Autophagy plays important roles in both innate and adaptive immune responses. However, the possible functional link between cocaine and autophagy has not been explore...

journal_title：Autophagy

authors： Guo ML,Liao K,Periyasamy P,Yang L,Cai Y,Callen SE,Buch S

更新日期：2015-01-01 00:00:00

abstract：:Formation of the autophagosome is likely the most complex step of macroautophagy, and indeed it is the morphological and functional hallmark of this process; accordingly, it is critical to understand the corresponding molecular mechanism. Atg8 is the only known autophagy-related (Atg) protein required for autophagosom...

journal_title：Autophagy

authors： Nair U,Yen WL,Mari M,Cao Y,Xie Z,Baba M,Reggiori F,Klionsky DJ

更新日期：2012-05-01 00:00:00

abstract：:Bulk degradation and nutrient recycling are events associated with autophagy. The core components of the autophagy machinery have been elucidated recently using molecular and genetic approaches. In particular, two ubiquitin-like proteins, ATG8 and ATG12, which conjugate with phosphatidylethanolamine (PE) and ATG5, res...

journal_title：Autophagy

authors： Xiao S,Chye ML

更新日期：2010-08-01 00:00:00

abstract：:Oxidative stress (OS) stimulates autophagy in different cellular systems, but it remains controversial if this rule can be generalized. We have analyzed the effect of chronic OS induced by the parkinsonian toxin paraquat (PQ) on autophagy in astrocytoma cells and primary astrocytes, which represent the first cellular ...

journal_title：Autophagy

authors： Janda E,Lascala A,Carresi C,Parafati M,Aprigliano S,Russo V,Savoia C,Ziviani E,Musolino V,Morani F,Isidoro C,Mollace V

更新日期：2015-01-01 00:00:00

abstract：:Autophagy is one of the survival processes of cancer cells, especially in stressful conditions such as starvation, hypoxia and chemotherapeutic agents. However, its roles in tumor survival have not yet been fully elucidated. Here, we found for the first time that JAK2/STAT3 was activated in HeLa cells when they were s...

journal_title：Autophagy

authors： Yoon S,Woo SU,Kang JH,Kim K,Kwon MH,Park S,Shin HJ,Gwak HS,Chwae YJ

更新日期：2010-11-01 00:00:00

abstract：:One of the major side effects of cisplatin chemotherapy is toxic acute kidney injury due to preferential accumulation of cisplatin in renal proximal tubule epithelial cells and the subsequent injury to these cells. Apoptosis is known as a major mechanism of cisplatin-induced cell death in renal tubular cells. We have ...

journal_title：Autophagy

authors： Kaushal GP,Kaushal V,Herzog C,Yang C

更新日期：2008-07-01 00:00:00

abstract：:Cargo-based assays have proven invaluable in the study of macroautophagy in yeast and mammalian cells. Proteomic analysis of autolysosomes identified the metabolic enzyme, betaine homocysteine methyltransferase (BHMT), as a potential cargo-based, end-point marker for mammalian macroautophagy. To test whether degradati...

journal_title：Autophagy

authors： Mercer CA,Kaliappan A,Dennis PB

更新日期：2008-02-01 00:00:00

abstract：:Recent evidence has indicated that the lysosome is able to act as a signaling organelle that senses nutrient availability and generates an adaptive response that is important for cellular homeostasis. We recently discovered another example of lysosomal signaling where lysosomal calcium release activates the master aut...

journal_title：Autophagy

authors： Medina DL,Ballabio A

更新日期：2015-01-01 00:00:00

abstract：:Thioglycolate-elicited macrophages exhibit abundant conjugation of LC3 with PE (LC3-II). Among other autophagy-related (ATG) proteins, it is proposed that, like in yeast, both ATG5 and ATG7 are essential for LC3 conjugation. Using atg5-deficient ( -/- ) and atg7-/- macroph...

journal_title：Autophagy

authors： Vujić N,Bradić I,Goeritzer M,Kuentzel KB,Rainer S,Kratky D,Radović B

更新日期：2021-01-18 00:00:00

abstract：:Numerous studies have reported that inhibition of MTOR (mechanistic target of rapamycin kinase) clearly reduces Alzheimer disease neuropathological hallmarks in mouse models. This has resulted in calls for the use of the MTOR inhibitor rapamycin for the treatment of dementia in humans. Unfortunately, intervention with...

journal_title：Autophagy

authors： Carosi JM,Sargeant TJ

更新日期：2019-08-01 00:00:00

abstract：:Recent studies have suggested that autophagy is involved in a neural death pathway following cerebral ischemia. In vivo detection of autophagy could be important for evaluating ischemic neural cell damage for human stroke patients. Using novel green fluorescent protein (GFP)-fused microtubule-associated protein 1 ligh...

journal_title：Autophagy

authors： Tian F,Deguchi K,Yamashita T,Ohta Y,Morimoto N,Shang J,Zhang X,Liu N,Ikeda Y,Matsuura T,Abe K

更新日期：2010-11-01 00:00:00

abstract：:Inhibition of the autophagic pathway has recently revealed promising results in increasing pro-death activity of multiple cancer therapeutics. Here, we discuss our findings regarding the autophagy-blocking and anti-neoplastic effects of a synthetic sphingosine analog, FTY720, in mantle cell lymphoma (MCL). We also emp...

journal_title：Autophagy

authors： Alinari L,Baiocchi RA,Praetorius-Ibba M

更新日期：2012-03-01 00:00:00