Abstract:
PURPOSE:L-Theanine is the major free amino acid present in tea (Camellia sinensis L.). The effects of several tea constituents on male reproduction have been investigated, but L-theanine has been overlooked. Sertoli cells (SCs) are essential for the physical and nutritional support of germ cells. In this study, we aimed to investigate the ability of L-theanine to modulate important mechanisms of human SCs (hSCs) metabolism, mitochondrial function and oxidative profile, which are essential to prevent or counteract spermatogenesis disruption in several health conditions. METHODS:We evaluated the effect of a dose of L-theanine attained by tea intake (5 μM) or a pharmacological dose (50 μM) on the metabolism (proton nuclear magnetic resonance and Western blot), mitochondrial functionality (protein expression of mitochondrial complexes and JC1 ratio) and oxidative profile (carbonyl levels, nitration and lipid peroxidation) of cultured hSCs. RESULTS:Exposure of hSCs to 50 µM of L-theanine increased cell proliferation and glucose consumption. In response to this metabolic adaptation, there was an increase in mitochondrial membrane potential, which may compromise the prooxidant-antioxidant balance. Still, no alterations were observed regarding the oxidative damages. CONCLUSIONS:A pharmacological dose of L-theanine (50 µM) prompts an increase in hSCs proliferation and a higher glucose metabolization to sustain the pool of Krebs cycle intermediates, which are crucial for cellular bioenergetics and biosynthesis. This study suggests an interplay between glycolysis and glutaminolysis in the regulation of hSCs metabolism.
journal_name
Eur J Nutrjournal_title
European journal of nutritionauthors
Dias TR,Bernardino RL,Alves MG,Silva J,Barros A,Sousa M,Casal S,Silva BM,Oliveira PFdoi
10.1007/s00394-019-01999-2subject
Has Abstractpub_date
2019-10-01 00:00:00pages
2961-2970issue
7eissn
1436-6207issn
1436-6215pii
10.1007/s00394-019-01999-2journal_volume
58pub_type
杂志文章abstract:PURPOSE:The interaction between genetics and diet may explain the present disagreement in the protective role of vitamin intake on cardiovascular disease. We cross-sectionally assessed the interaction of habitual dietary intake of β-carotene, vitamin C, folate, and vitamin E with single nucleotide polymorphisms (SNPs) ...
journal_title:European journal of nutrition
pub_type: 杂志文章,多中心研究
doi:10.1007/s00394-015-0896-z
更新日期:2016-03-01 00:00:00
abstract:PURPOSE:Inadequate iodine intake may result in iodine deficiency disorders (IDD). Thus, for more than 50 years, policies for the regulation of salt fortification with iodine have existed in Brazil. In 2003, a study on 6-14-year-old schoolchildren from regions of the state of São Paulo showed a median urinary iodine con...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-011-0239-7
更新日期:2012-08-01 00:00:00
abstract:BACKGROUND:Inulin has been suggested to have beneficial effects on lipids, especially on triglyceridemia. Few data are available about the effects of inulin on Lipoprotein(a), a low-density lipoprotein-like particle considered as an independent risk factor for atherosclerosis. Adding inulin to pasta could be a preventi...
journal_title:European journal of nutrition
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00394-008-0748-1
更新日期:2008-12-01 00:00:00
abstract:BACKGROUND:Inulin-type fructans or chains with mainly beta[2-1] linked fructose molecules escape the ingestion procedure in the small intestine and are fermented by the microflora, and are known to increase colonic absorption of minerals in animals. The fermentation rate in the large bowel into short-chain fatty acids ...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-003-0390-x
更新日期:2003-04-01 00:00:00
abstract:PURPOSE:Manifestation of allergic disease depends on genetic predisposition, diet and commensal microbiota. Genetic polymorphism of mothers determines their breast milk glycan composition. One major determinant is the fucosyltransferase 2 (FUT2, secretor gene) that was shown to be linked to commensal microbiota establi...
journal_title:European journal of nutrition
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00394-016-1180-6
更新日期:2017-04-01 00:00:00
abstract:PURPOSE:Sesamin, a polyphenolic compound found in sesame seeds, has been reported to exert a variety of beneficial health effects. We have previously reported that sesamin increases the lifespan of Caenorhabditis elegans. In this study, we investigated the molecular mechanisms underlying the longevity effect of sesamin...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-017-1396-0
更新日期:2018-04-01 00:00:00
abstract:BACKGROUND:Iodine fortification programs have been applied in many iodine deficient regions. Iodine excess is also unfavourable, and it is recommended to monitor iodine status by measuring urinary iodine concentration (UIC). The number of samples needed in such monitoring depends on the variation in UIC. However, it is...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-013-0551-5
更新日期:2014-01-01 00:00:00
abstract:PURPOSE:The effect of 40 μg (1,600 IU) per day of vitamin D(3) on serum 25-hydroxyvitamin D (25(OH)D) and markers of bone and mineral metabolism was evaluated. METHODS:This intervention study was designed as a double-blind randomised controlled trial. Forty-five community-dwelling subjects (32 females), age 55-84 year...
journal_title:European journal of nutrition
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00394-011-0271-7
更新日期:2012-12-01 00:00:00
abstract:BACKGROUND:Lutein is a carotenoid that may play a role in eye health. Human milk typically contains higher concentrations of lutein than infant formula. Preliminary data suggest there are differences in serum lutein concentrations between breastfed and formula-fed infants. AIM OF THE STUDY:To measure the serum lutein ...
journal_title:European journal of nutrition
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00394-009-0047-5
更新日期:2010-02-01 00:00:00
abstract:PURPOSE:In this study, we sought to find the effects and mechanisms of probiotic Lactobacillus casei Zhang (L. casei Zhang) on the pro-inflammatory cytokine production and hepatic inflammatory response in a rat model of acute liver failure induced by lipopolysaccharide (LPS) and d-galactosamine (GalN). METHODS:Male Wi...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-015-0904-3
更新日期:2016-03-01 00:00:00
abstract:PURPOSE:The enzymes encoded by fatty acid desaturases (FADS) genes determine the desaturation of long-chain polyunsaturated fatty acids (LCPUFA). We investigated if haplotype and single nucleotide polymorphisms (SNPs) in FADS gene cluster can influence LCPUFA status in infants who received either fish oil or placebo su...
journal_title:European journal of nutrition
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00394-017-1529-5
更新日期:2018-10-01 00:00:00
abstract:PURPOSE:It is believed that breakfast is an important meal due to its effect on appetite control and cognitive performance, yet little evidence exists to support this hypothesis. METHODS:Using a crossover design, 33 healthy undergraduates (aged 22 ± 2 years with a BMI of 23.5 ± 1.7 kg/m2) were randomized one of four b...
journal_title:European journal of nutrition
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00394-016-1254-5
更新日期:2017-09-01 00:00:00
abstract:PURPOSE:To compare the glycaemic response of whole grain oat kernels and pearled oats cooked under normal pressure or higher pressure and examine whether replacing half of the rice in a given meal with either of the two oat grains could make a difference in acute glycaemic responses. METHODS:In a randomised crossover ...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-019-02083-5
更新日期:2020-09-01 00:00:00
abstract:PURPOSE:A meal replacement may be an effective strategy in the management of obesity to increase antioxidant intake, attenuating oxidative stress and inflammation. In the present study, we investigated the efficacy of a new nutritional supplement to reduce metabolic risk parameters in obese women. METHODS:In a randomi...
journal_title:European journal of nutrition
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s00394-013-0598-3
更新日期:2014-04-01 00:00:00
abstract:PURPOSE:Several studies have demonstrated the properties of hydroxytyrosol, a phenolic compound present in olive oils and olives with a well-characterized impact on human health. Nevertheless, some knowledge gaps remain on its bioavailability and metabolism; overall concerning to the real rate per cent of absorption an...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-020-02295-0
更新日期:2020-06-10 00:00:00
abstract:PURPOSE:Adipose tissue is now recognized as a highly active metabolic and endocrine organ. Our aim was to investigate the effect of the dietary fat on the two main adipose tissue functions, endocrine and lipid store, by analyzing the adipose tissue gene expression from metabolic syndrome patients. METHODS:A randomized...
journal_title:European journal of nutrition
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00394-013-0570-2
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Dietary polyphenols have been reported to have a variety of biological actions, including anticarcinogenic and antioxidant activities. AIM OF THE STUDY:In the present study we investigated the protective effect of dietary polyphenols against N-nitrosodimethylamine (NDMA), N-nitrosopyrrolidine (NPYR) and ben...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-008-0751-6
更新日期:2008-12-01 00:00:00
abstract:BACKGROUND:Bioavailability of fat-soluble vitamins from conventional oral supplements is insufficient in some conditions in which fat digestion and absorption are chronically impaired (e. g. cystic fibrosis). AIM OF THE STUDY:We used a water-soluble form of fatsoluble vitamin E (AQUANOVA solubilisate) to create a nutr...
journal_title:European journal of nutrition
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00394-005-0556-9
更新日期:2006-02-01 00:00:00
abstract:BACKGROUND:Glutathione peroxidase 1 (GPx1) is an antioxidant selenoenzyme that protects the cells against reactive oxygen species. Its activity depends on the concentration of selenium (Se) which is present in the active centre of the enzyme. The genetic polymorphism of GPx1 encoding gene (GPx1) associated with the pro...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-009-0023-0
更新日期:2009-09-01 00:00:00
abstract:BACKGROUND:While naturally occurring inulin has anti-hyperlipidemic effects in animals and humans, health effects of synthetic inulin with different degrees of fructose polymerization remain poorly understood. AIM OF THE STUDY:Our study aimed at distinguishing health effects of synthetic inulin with different degrees ...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-008-0713-z
更新日期:2008-06-01 00:00:00
abstract:PURPOSE:This study aimed to assess the effects of folic acid (FA) combined with a docosahexaenoic acid (DHA) intervention on the cognitive function and inflammatory cytokines in elderly subjects with mild cognitive impairment (MCI). METHODS:This randomized, double-blind, placebo-controlled trial recruited 240 individu...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-020-02373-3
更新日期:2020-08-28 00:00:00
abstract:PURPOSE:Coffee is known to contain phytochemicals with antioxidant potential. The aim of this study was to investigate possible antioxidant effects of coffee in healthy human volunteers. METHODS:A placebo-controlled intervention trial was carried out on 160 healthy human subjects, randomised into three groups, receivi...
journal_title:European journal of nutrition
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00394-016-1336-4
更新日期:2018-03-01 00:00:00
abstract:PURPOSE:Concentration of carotenoids in the serum is a biomarker of mainly vegetable and fruit consumption. However, the levels of carotenoids in humans may decline with age and can also depend on body fat, alcohol consumption, and cigarette smoking. METHOD:Food intake and characteristics of 159 randomly chosen women ...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-013-0493-y
更新日期:2013-12-01 00:00:00
abstract:BACKGOUND:Theobromine, a component of cocoa, may favorably affect conventional lipid-related cardiovascular risk markers, but effects on flow-mediated dilation (FMD) and other vascular function markers are not known. OBJECTIVE:To evaluate the effects of 4-week theobromine consumption (500 mg/day) on fasting and postpr...
journal_title:European journal of nutrition
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00394-018-1612-6
更新日期:2019-04-01 00:00:00
abstract:PURPOSE:It is unclear if midlife consumption of dairy and soy food intake, and their components of calcium and isoflavones (in soy), is related to cognitive impairment in elderly. METHODS:We used baseline data on lifestyle and habitual diet of 16,948 participants collected during their recruitment into the Singapore C...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-019-02010-8
更新日期:2020-06-01 00:00:00
abstract:PURPOSE:Animal and laboratory studies suggest that long-chain omega-3 (n-3) fatty acids, a type of polyunsaturated fat found in fatty fish, may protect against carcinogenesis, but human studies on dietary intake of polyunsaturated fats and fish with endometrial cancer risk show mixed results. METHODS:We evaluated the ...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-012-0436-z
更新日期:2013-04-01 00:00:00
abstract:PURPOSE:The impact of diet on endurance performance and cognitive function has been extensively researched in controlled settings, but there are limited observational data in field situations. This study examines relationships between nutrient intake and cognitive function following endurance exercise amongst a group o...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-012-0364-y
更新日期:2013-03-01 00:00:00
abstract:PURPOSE:Glucosinolates/isothiocyanates are an established class of naturally occurring chemopreventive agents, a principal mechanism of action being to limit the generation of genotoxic metabolites of chemical carcinogens, as a result of modulation of cytochrome P450 and phase II detoxification enzymes. The objective o...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-012-0397-2
更新日期:2013-04-01 00:00:00
abstract::In the Original publication of the article Fig. 1 was published incorrectly. The correct figure is given below. The original article has been corrected. ...
journal_title:European journal of nutrition
pub_type: 杂志文章,已发布勘误
doi:10.1007/s00394-018-1800-4
更新日期:2018-10-01 00:00:00
abstract:BACKGROUND:Vegetables and fruits are rich sources of a variety of nutrients, including vitamins (E and C), trace minerals, and dietary fibers, and many other classes of biologically active compounds such as carotenoids and polyphenols, which are often assumed to protect against degenerative pathologies such as cardiova...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-006-0594-y
更新日期:2006-08-01 00:00:00