Abstract:
:To date, the combination of histological sectioning, staining, and microscopic assessment of the 2D sections is still the golden standard for structural and compositional analysis of biological tissues. X-ray microfocus computed tomography (microCT) is an emerging 3D imaging technique with high potential for 3D structural analysis of biological tissues with a complex and heterogeneous 3D structure, such as the trabecular bone. However, its use has been mostly limited to mineralized tissues because of the inherently low X-ray absorption of soft tissues. To achieve sufficient X-ray attenuation, chemical compounds containing high atomic number elements that bind to soft tissues have been recently adopted as contrast agents (CAs) for contrast-enhanced microCT (CE-CT); this novel technique is very promising for quantitative "virtual" 3D anatomical pathology of both mineralized and soft biological tissues. In this paper, we provided a review of the advances in CE-CT since the very first reports on the technology to date. Perfusion CAs for in vivo imaging have not been discussed, as the focus of this review was on CAs that bind to the tissue of interest and that are, thus, used for ex vivo imaging of biological tissues. As CE-CT has mostly been applied for the characterization of musculoskeletal tissues, we have put specific emphasis on these tissues. Advantages and limitations of multiple CAs for different musculoskeletal tissues have been highlighted, and their reproducibility has been discussed. Additionally, the advantages of the "full" 3D CE-CT information have been pinpointed, and its importance for more detailed structural, spatial, and functional characterization of the tissues of interest has been shown. Finally, the remaining challenges that are still hampering a broader adoption of CE-CT have been highlighted, and suggestions have been made to move the field of CE-CT imaging one step further towards a standard accepted tool for quantitative virtual 3D anatomical pathology.
journal_name
Contrast Media Mol Imagingjournal_title
Contrast media & molecular imagingauthors
de Bournonville S,Vangrunderbeeck S,Kerckhofs Gdoi
10.1155/2019/8617406subject
Has Abstractpub_date
2019-02-28 00:00:00pages
8617406eissn
1555-4309issn
1555-4317journal_volume
2019pub_type
杂志文章,评审abstract::A new high-throughput MRI method for screening chemical exchange saturation transfer (CEST) agents is demonstrated, allowing simultaneous testing of multiple samples with minimal attention to sample configuration and shimming of the main magnetic field (B(0)). This approach, which is applicable to diamagnetic, paramag...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.383
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abstract::Various contrast agents (Magnevist, Gadovist and Multihance) loaded into polymeric nanocapsules were synthesized by the inverse miniemulsion technique. The relaxivity of the resultant contrast agents was assessed at 1.5 T magnetic field strength. The ionic relaxivity of the contrast agents could at least be maintained...
journal_title:Contrast media & molecular imaging
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abstract::For in vivo applications of magnetically labeled stem cells, biological effects of the labeling procedure have to be precluded. This study evaluates the effect of different ferucarbotran cell labeling protocols on chondrogenic differentiation of human mesenchymal stem cells (hMSC) as well as their implications for MR ...
journal_title:Contrast media & molecular imaging
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doi:10.1002/cmmi.276
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.1705
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abstract::Chemical exchange saturation transfer (CEST) MRI is sensitive to dilute proteins and peptides as well as microenvironmental properties. However, the complexity of the CEST MRI effect, which varies with the labile proton content, exchange rate and experimental conditions, underscores the need for developing quantitativ...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.1569
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journal_title:Contrast media & molecular imaging
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.1473
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1155/2019/4087895
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.289
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.376
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章,评审
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.1695
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journal_title:Contrast media & molecular imaging
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abstract::Glioblastoma multiforme is the most aggressive form of brain tumor. Early and accurate diagnosis of glioma and its borders is an important step for its successful treatment. One of the promising targets for selective visualization of glioma and its margins is connexin 43 (Cx43), which is highly expressed in reactive a...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.262
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.432
更新日期:2011-07-01 00:00:00
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.414
更新日期:2011-05-01 00:00:00
abstract::Stem cells, cancer cells and immune cells were labeled by co-incubation with a new ultra-small iron oxide nanoparticle called Molday ION Rhodamine-B (MIRB). Iron staining, fluorescence imaging, transmission electron microscopy and flow cytometry were used to assess cell viability, function and labeling efficiency. Thi...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.456
更新日期:2011-11-01 00:00:00
abstract::A novel tumor stroma targeting and membrane-penetrating cyclic peptide, named iCREKA, was designed and labeled by fluorescein isothiocyanate (FITC) and positron emitter 18F to build the tumor-targeting tracers. The FITC-iCREKA was proved to have significantly higher cellular uptake in the glioma U87 cells in the prese...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1155/2018/7929617
更新日期:2018-03-04 00:00:00
abstract::Photoacoustic imaging is an emerging imaging modality for noninvasive detection of tissue structural and functional anomalies. Multifunctional microbubbles (MBs) and nanobubbles (NBs) are contrast agents integrating multiple disease-targeting, imaging and therapeutic functions. Multifunctional MBs and NBs represent an...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章,评审
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journal_title:Contrast media & molecular imaging
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journal_title:Contrast media & molecular imaging
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
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abstract:Purpose:Several meta-analyses have reported data about the diagnostic performance of positron emission tomography or positron emission tomography/computed tomography (PET or PET/CT) with different radiotracers in patients with suspicious lung cancer (LC) or pleural tumours (PT). This review article aims at providing an...
journal_title:Contrast media & molecular imaging
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journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1155/2018/8046541
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abstract::Recent reports have claimed a superior performance of HEPES buffer in comparison to alternative buffer systems for (67/68) Ga labeling in aqueous media. In this paper we report spectroscopic ((1) H and (71) Ga NMR), radiochemical, mass spectrometry and theoretical modeling studies on the Ga(3+)/HEPES system (HEPES = N...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.1517
更新日期:2013-05-01 00:00:00
abstract::Glioblastoma (GBM) is the most common primary malignant type of brain neoplasm in adults and carries a dismal prognosis. The current standard of care for GBM is surgical excision followed by radiation therapy (RT) with concurrent and adjuvant temozolomide-based chemotherapy (TMZ) by six additional cycles. In addition,...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章,评审
doi:10.1155/2018/6828396
更新日期:2018-12-02 00:00:00
abstract:Background:To evaluate the clinical and prognostic value of PET/CT with combination of 68Ga-DOTATATE and 18F-FDG in gastroenteropancreatic neuroendocrine neoplasms (GEP-NENs). Method:83 patients of GEP-NENs who underwent 68Ga-DOTATATE and 18F-FDG PET/CT were enrolled between June 2013 and December 2016. Well-different...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1155/2018/2340389
更新日期:2018-02-26 00:00:00
abstract::MRI cell tracking is a promising technique to track various cell types (stem cells, tumor cells, etc.) in living animals. Usually, cells are incubated with iron oxides (T(2) contrast agent) in order to take up the particles before being injected in vivo. Iron oxide quantification is important in such studies for valid...
journal_title:Contrast media & molecular imaging
pub_type: 杂志文章
doi:10.1002/cmmi.497
更新日期:2012-05-01 00:00:00