Transfer of a chromosomal locus responsible for mucoid colony morphology in Pseudomonas aeruginosa isolated from cystic fibrosis patients to P. aeruginosa PAO.

abstract：PURPOSE:In an increasing number of cases the last therapeutic option for treatment of carbapenem-resistant Enterobacteriaceae is colistin. As the detection of colistin resistance is problematic and time-consuming, it is desirable to find a rapid and reliable test. The rapid polymyxin NP test developed by Nordmann et al...

journal_title：Journal of medical microbiology

authors： Belda-Orlowski A,Pfennigwerth N,Gatermann SG,Korte-Berwanger M

更新日期：2019-08-01 00:00:00

abstract：:We report what we believe is the first reported case of Streptococcus mutans endocarditis complicated by vertebral discitis. The case is particularly interesting and topical as it occurred in a patient with pre-existing cardiac valvular disease who had recently had a dental procedure without antibiotic prophylaxis fol...

journal_title：Journal of medical microbiology

authors： Biswas S,Bowler ICJW,Bunch C,Prendergast B,Webster DP

更新日期：2010-10-01 00:00:00

abstract：:Spirochaetes were demonstrated in material from patients with Lyme disease by short-time high-concentrate silver impregnation after treatment with amylase. Removal of mucoid material was essential to visualise Borrelia burgdorferi. Lyme spirochaetes were demonstrated in material from 23 patients with Lyme disease--ery...

journal_title：Journal of medical microbiology

authors： De Koning J,Bosma RB,Hoogkamp-Korstanje JA

更新日期：1987-05-01 00:00:00

abstract：:Aim. The aim of this work was to characterize the response of Candida albicans to atorvastatin, and to assess its in vivo antifungal capability.Methodology. The effect of atorvastatin on the growth and viability of C. albicans was assessed. The ability of the statin to alter cell permeability was quantified by measuri...

journal_title：Journal of medical microbiology

authors： Ajdidi A,Sheehan G,Abu Elteen K,Kavanagh K

更新日期：2019-10-01 00:00:00

abstract：:A serial multiplex PCR approach was reformulated for pneumococcal serotyping to test 153 clinical isolates from children in Mozambique. This approach identified serotypes in 139 (90.8%) of 153 isolates; 126 (82.4%) were identified within two reactions. This approach in developing countries would require minimal traini...

journal_title：Journal of medical microbiology

authors： Morais L,Carvalho MDG,Roca A,Flannery B,Mandomando I,Soriano-Gabarró M,Sigauque B,Alonso P,Beall B

更新日期：2007-09-01 00:00:00

abstract：:The PCR was used to detect the presence of Helicobacter pylori in subgingival plaque samples from patients with adult periodontitis. Primers based upon the 16S ribosomal RNA (rRNA) gene sequence of H. pylori were used in a single round of PCR to amplify a 295-bp DNA fragment and the identity of the amplified products ...

journal_title：Journal of medical microbiology

authors： Riggio MP,Lennon A

更新日期：1999-03-01 00:00:00

abstract：:The genetic diversity between major meticillin-resistant Staphylococcus aureus (MRSA) lineages was probed using fluorescent amplified fragment length polymorphism (FAFLP) as a random genome sampling tool. Genomic DNA was digested with endonucleases BglII and Csp6I and a subset of the restricted fragments were amplifie...

journal_title：Journal of medical microbiology

authors： Desai D,Ellington MJ,Arnold C,Desai M

更新日期：2012-12-01 00:00:00

abstract：:Rickettsia rickettsii has limited adverse effects on its arthropod vector, but causes severe disease in man. To model differences in host-parasite interaction, R. rickettsii growth and protein expression were examined at temperatures reflective of host environment in the tick cell lines DALBE3 and IDE2, the human endo...

journal_title：Journal of medical microbiology

authors： Policastro PF,Munderloh UG,Fischer ER,Hackstadt T

更新日期：1997-10-01 00:00:00

abstract：:The lipopolysaccharides (LPS) of the 10 species of the genus Bacteroides (sensu stricto) were extracted by the proteinase K method and their resolution compared by several methods of polyacrylamide gel electrophoresis (PAGE). These included sodium dodecyl sulphate (SDS)-PAGE with and without urea, polyacrylamide gradi...

journal_title：Journal of medical microbiology

authors： Maskell JP

更新日期：1991-05-01 00:00:00

abstract：:Introduction.Mycoplasma genitalium is a sexually transmitted pathogen with increasing resistance to first- and second-line antimicrobials. The 'near-patient test' ResistancePlus MG FleXible (SpeeDx) detects M. genitalium plus four macrolide resistance mutations (MRMs), facilitating same-day patient follow up.Hypothesi...

journal_title：Journal of medical microbiology

authors： Murray GL,Doyle M,Bodiyabadu K,Vodstrcil LA,Garland SM,Danielewski J,Machalek DA,McGuinness C,Plummer EL,De Petra V,Williamson DA,Bradshaw CS

更新日期：2021-01-01 00:00:00

abstract：:Campylobacter jejuni is a frequent cause of infectious diarrhoea and is increasingly recognized as a trigger for late-onset complications. The poor standardization of commonly used serological tests might explain the conflicting results regarding the frequency of antecedent C. jejuni infections in defined patient grou...

journal_title：Journal of medical microbiology

authors： Schmidt-Ott R,Brass F,Scholz C,Werner C,Groß U

更新日期：2005-08-01 00:00:00

abstract：:Human gastric epithelial immortalized GES-1 cells were infected with spiral and coccoid Helicobacter pylori. Scanning electron microscopy was used to determine the ability of the two forms of H. pylori to adhere to GES-1 cells. GES-1 cell apoptosis induced by coccoid and spiral H. pylori was analysed using flow cytome...

journal_title：Journal of medical microbiology

authors： Liu ZF,Chen CY,Tang W,Zhang JY,Gong YQ,Jia JH

更新日期：2006-08-01 00:00:00

abstract：:The distribution of hepatitis C virus (HCV) genotypes among Egyptian patients positive for anti-HCV was determined and their influence, when combined with neu-oncoprotein overexpression, on the development of hepatocellular carcinoma (HCC) was examined. The study groups included asymptomatic carriers (ASC) and patient...

journal_title：Journal of medical microbiology

authors： Zekri AN,Bahnassy AA,Shaarawy SM,Mansour OA,Maduar MA,Khaled HM,El-Ahmadi O

更新日期：2000-01-01 00:00:00

abstract：:The advent of next-generation sequencing technology has revolutionized the field of prokaryotic genetics and genomics by allowing interrogation of entire genomes, transcriptomes and global transcription factor binding profiles. As more studies employing these techniques have been performed, the state of the art regard...

journal_title：Journal of medical microbiology

authors： Valli RXE,Lyng M,Kirkpatrick CL

更新日期：2020-02-01 00:00:00

abstract：:Limited digestion of staphylococcal enterotoxin B (SEB) with trypsin resulted in the generation of a 12-Kda amino-terminal fragment and a 17-Kda carboxy-terminal fragment which were isolated by preparative iso-electric focusing. The carboxy-terminal fragment exhibited significant mitogenic activity for murine splenocy...

journal_title：Journal of medical microbiology

authors： Binek M,Newcomb JR,Rogers CM,Rogers TJ

更新日期：1992-03-01 00:00:00

abstract：:A mouse enterohaemorrhagic Escherichia coli (EHEC) infection model was developed with a combination of germ-free (GF) mice and hyper-toxigenic EHEC (HT-EHEC) O157:H7 strain 6. The HT-EHEC strain 6 produced both Shiga-like toxin (SLT)-1 1.0 microg/mI and SLT-2 8.2 microg/ml in vitro. Eight-week-old germ-free mice were ...

journal_title：Journal of medical microbiology

authors： Taguchi H,Takahashi M,Yamaguchi H,Osaki T,Komatsu A,Fujioka Y,Kamiya S

更新日期：2002-04-01 00:00:00

abstract：:The growth inhibiting activity of transferrins, citrate, 2-2' dipyridyl and desferrioxamine methanesulphonate towards Legionella spp. and their serogroups was investigated. The inhibitory activity of all these compounds depended upon the iron-free state of the molecules and was abolished by saturation with iron. No ba...

journal_title：Journal of medical microbiology

authors： Goldoni P,Visca P,Pastoris MC,Valenti P,Orsi N

更新日期：1991-02-01 00:00:00

abstract：:The enzymic activity of 29 Haemophilus ducreyi strains on 28 substrates is described. The results are compared with those of seven other authors. There is agreement only about the presence of alkaline phosphatase and arginine aminopeptidase and the lack of glycosidases. Possible reasons for the contradictions in the e...

journal_title：Journal of medical microbiology

authors： Sturm AW,Zanen HC

更新日期：1984-10-01 00:00:00

abstract：:Rapid identification of isolates in positive blood cultures are of great importance to secure correct treatment of septicaemic patients. As antimicrobial resistance is increasing, rapid detection of resistance is crucial. Carbapenem resistance in Bacteroides fragilis associated with cfiA-encoded class B metallo-beta-l...

journal_title：Journal of medical microbiology

authors： Johansson Å,Nagy E,Sóki J

更新日期：2014-08-01 00:00:00

abstract：:Campylobacter-like organisms, isolated from the gastric antrum of Rhesus monkeys, were compared with Campylobacter jejuni and C. pylori. They were similar to C. pylori by light microscopy, in ultrastructural morphology, in enzymic, fatty-acid-methyl-ester, and protein-profile analysis, and in antigenic reactivity with...

journal_title：Journal of medical microbiology

authors： Newell DG,Hudson MJ,Baskerville A

更新日期：1988-09-01 00:00:00

abstract：:Campylobacter strains isolated from the same stool sample were characterised by determination of biochemical properties and both heat-labile (Lior) and heat-stable (Lauwers) serotypes. In six of 60 campylobacter-infected stools, two or three strains differing in Lior-serotype were isolated from the same stool. In four...

journal_title：Journal of medical microbiology

authors： Zöllner B,Wuthe HH

更新日期：1993-01-01 00:00:00

abstract：PURPOSE:With an increase in the numbers of bacterial isolates resistant to first-line antibiotics, there has been a revival in the use of older drugs including fosfomycin with novel mechanisms of action. We aimed to investigate the prevalence and genotypic nature of fosfomycin resistance in Escherichia coli from urinar...

journal_title：Journal of medical microbiology

authors： Cottell JL,Webber MA

更新日期：2019-02-01 00:00:00

abstract：:The opsonic requirements of Helicobacter pylori were investigated in a series of experiments with human polymorphonuclear leucocytes (PMNL). Pre-incubation of H. pylori with pooled normal human serum (NHS) in concentrations of 5-20% significantly increased the uptake of radiolabelled bacteria by PMNL. Treatment of the...

journal_title：Journal of medical microbiology

authors： McKinlay AW,Young A,Russell RI,Gemmell CG

更新日期：1993-03-01 00:00:00

abstract：:Two toxigenic Corynebacterium ulcerans isolates recovered from pharyngeal swabs of two patients from the same hospital in Japan during 2001-2002 were characterized by PFGE and ribotyping. Toxin production in different culture media was examined and serological analysis of patient sera was performed. The two isolates c...

journal_title：Journal of medical microbiology

authors： Komiya T,Seto Y,De Zoysa A,Iwaki M,Hatanaka A,Tsunoda A,Arakawa Y,Kozaki S,Takahashi M

更新日期：2010-12-01 00:00:00

abstract：:The incidence of candidaemia among immunocompromised patients in Malaysia is increasing at an alarming rate. Isolation of clinical strains that are resistant to fluconazole has also risen markedly. We report here the repeated isolation of Candida tropicalis from the blood of a neonatal patient with Hirschsprung's dise...

journal_title：Journal of medical microbiology

authors： Chong PP,Chieng DC,Low LY,Hafeez A,Shamsudin MN,Seow HF,Ng KP

更新日期：2006-04-01 00:00:00

abstract：:Introduction. In May-June 2018, an outbreak of campylobacteriosis involved students and school staff from kindergartens and primary schools in Pescara, southern Italy.Aim. We present details of the epidemiological and microbiological investigation, and the findings of the analytical study, as well as the implemented c...

journal_title：Journal of medical microbiology

authors： Sorgentone S,Busani L,Calistri P,Robuffo G,Bellino S,Acciari V,Ferri M,Graziani C,Antoci S,Lodi F,Alfonsi V,Cammà C,Fazii P,Andrianou X,Cito F,Lombardi G,Centorotola G,D'Amario M,D'Alterio N,Savini V,De Massis F,

更新日期：2021-01-21 00:00:00

abstract：:Terminal restriction fragment-length polymorphism (T-RFLP) analysis was applied to characterize oral bacterial flora in saliva from 18 healthy subjects and 18 patients with periodontitis. The 16S rRNA genes (rDNAs) of oral bacteria and spirochaetes in saliva were amplified by PCR with a 6'carboxy-fluorescein (6-FAM)-l...

journal_title：Journal of medical microbiology

authors： Sakamoto M,Takeuchi Y,Umeda M,Ishikawa I,Benno Y

更新日期：2003-01-01 00:00:00

abstract：:In order to develop novel host/pathogen real-time PCR assays for routine diagnostic use, early gene expression patterns from both Epstein-Barr virus (EBV) and Raji cells were examined after inducing the lytic life cycle using 12-O-tetradecanoyl-13-phorbol ester and sodium butyrate. Real-time PCR identified several hig...

journal_title：Journal of medical microbiology

authors： Auburn H,Zuckerman M,Smith M

更新日期：2016-11-01 00:00:00

abstract：:The opsonic and protective capacities of rabbit antisera against Escherichia coli O, K and core-glycolipid cell-wall antigens were compared with specific antibody titres as measured by agglutination and enzyme-linked immunosorbent assay. Anti-O antisera were opsonic and protective against two noncapsulate strains. Onl...

journal_title：Journal of medical microbiology

authors： van Dijk WC,Verbrugh HA,van Erne-van der Tol ME,Peters R,Verhoef J

更新日期：1981-11-01 00:00:00

abstract：:Klebsiella pneumoniae meningitis has recently become an increasingly common cause of central nervous system infection. The invasion of bacteria within the subarachnoid space stimulates the release of pro-inflammatory cytokines and chemokines, triggering a host immune response. The aim of the present study was to evalu...

journal_title：Journal of medical microbiology

authors： Barichello T,Simões LR,Valvassori SS,Generoso JS,Aveline PEDV,Dominguini D,Elias SG,Vilela MC,Quevedo J,Teixeira AL

更新日期：2014-01-01 00:00:00