Localisation of the mitogenic epitope of staphylococcal enterotoxin B.

Abstract:

:Limited digestion of staphylococcal enterotoxin B (SEB) with trypsin resulted in the generation of a 12-Kda amino-terminal fragment and a 17-Kda carboxy-terminal fragment which were isolated by preparative iso-electric focusing. The carboxy-terminal fragment exhibited significant mitogenic activity for murine splenocytes, whereas the isolated amino-terminal fragment possessed little detectable mitogenic activity. Monoclonal antibodies (MAbs) specific for the carboxy-terminal fragment neutralised most of the mitogenic activity of both the intact toxin and the carboxy-terminal fragment. MAbs specific for the amino-terminal fragment had no detectable neutralising activity. These results support the hypothesis that the epitope(s) responsible for mitogenic activity is located in the carboxy-terminal region of SEB.

journal_name

J Med Microbiol

authors

Binek M,Newcomb JR,Rogers CM,Rogers TJ

doi

10.1099/00222615-36-3-155

keywords:

subject

Has Abstract

pub_date

1992-03-01 00:00:00

pages

156-63

issue

3

eissn

0022-2615

issn

1473-5644

journal_volume

36

pub_type

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