Transcriptome Analysis of Watermelon Leaves Reveals Candidate Genes Responsive to Cucumber green mottle mosaic virus Infection.

Abstract:

:Cucumber green mottle mosaic virus (CGMMV) is a member of the genus Tobamovirus, which cause diseases in cucurbits, especially watermelon. In watermelon, symptoms develop on the whole plant, including leaves, stems, peduncles, and fruit. To better understand the molecular mechanisms of watermelon early responses to CGMMV infection, a comparative transcriptome analysis of 24 h CGMMV-infected and mock-inoculated watermelon leaves was performed. A total of 1641 differently expressed genes (DEGs) were identified, with 886 DEGs upregulated and 755 DEGs downregulated after CGMMV infection. A functional analysis indicated that the DEGs were involved in photosynthesis, plant⁻pathogen interactions, secondary metabolism, and plant hormone signal transduction. In addition, a few transcription factor families, including WRKY, MYB, HLH, bZIP and NAC, were responsive to the CGMMV-induced stress. To confirm the high-throughput sequencing results, 15 DEGs were validated by qRT-PCR analysis. The results provide insights into the identification of candidate genes or pathways involved in the responses of watermelon leaves to CGMMV infection.

journal_name

Int J Mol Sci

authors

Sun Y,Fan M,He Y

doi

10.3390/ijms20030610

subject

Has Abstract

pub_date

2019-01-31 00:00:00

issue

3

issn

1422-0067

pii

ijms20030610

journal_volume

20

pub_type

杂志文章
  • Application of an Inter-Species Extrapolation Method for the Prediction of Drug Interactions between Propolis and Duloxetine in Humans.

    abstract::Duloxetine (DLX) is a potent drug investigated for the treatment of depression and urinary incontinence. DLX is extensively metabolized in the liver by two P450 isozymes, CYP2D6 and CYP1A2. Propolis (PPL) is one of the popular functional foods known to have effects on activities of CYPs, including CYP1A2. Due to the h...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21051862

    authors: Ngo TL,Lee CH,Han N,Back HM,Rhee SJ,Noh K,Yun HY,Kang W,Chae JW

    更新日期:2020-03-09 00:00:00

  • Hair Growth Promoting Effect of 4HGF Encapsulated with PGA Nanoparticles (PGA-4HGF) by β-Catenin Activation and Its Related Cell Cycle Molecules.

    abstract::Poly-γ-glutamic acid (γ-PGA)-based nanoparticles draw remarkable attention as drug delivery agents due to their controlled release characteristics, low toxicity, and biocompatibility. 4HGF is an herbal mixture of Phellinus linteus grown on germinated brown rice, Cordyceps militaris grown on germinated soybeans, Polygo...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20143447

    authors: Lee HJ,Kwon HK,Kim HS,Kim MI,Park HJ

    更新日期:2019-07-13 00:00:00

  • Epigenetics in Lewy Body Diseases: Impact on Gene Expression, Utility as a Biomarker, and Possibilities for Therapy.

    abstract::Lewy body disorders (LBD) include Parkinson's disease (PD) and dementia with Lewy bodies (DLB). They are synucleinopathies with a heterogeneous clinical manifestation. As a cause of neuropathological overlap with other neurodegenerative diseases, the establishment of a correct clinical diagnosis is still challenging, ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21134718

    authors: Urbizu A,Beyer K

    更新日期:2020-07-02 00:00:00

  • Treatment of Men with Central Hypogonadism: Alternatives for Testosterone Replacement Therapy.

    abstract::Central hypogonadism is a clinical condition, characterized by sexual symptoms and low serum testosterone levels, due to an impaired function of the hypothalamus or pituitary gland. Testosterone replacement therapy (TRT) is the standard treatment for hypogonadism, but it has some disadvantages. TRT is not a good optio...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms22010021

    authors: Ide V,Vanderschueren D,Antonio L

    更新日期:2020-12-22 00:00:00

  • Fractalkine/CX3CL1 in Neoplastic Processes.

    abstract::Fractalkine/CX3C chemokine ligand 1 (CX3CL1) is a chemokine involved in the anticancer function of lymphocytes-mainly NK cells, T cells and dendritic cells. Its increased levels in tumors improve the prognosis for cancer patients, although it is also associated with a poorer prognosis in some types of cancers, such as...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21103723

    authors: Korbecki J,Simińska D,Kojder K,Grochans S,Gutowska I,Chlubek D,Baranowska-Bosiacka I

    更新日期:2020-05-25 00:00:00

  • A new approach on estimation of solubility and n-octanol/water partition coefficient for organohalogen compounds.

    abstract::The aqueous solubility (logW) and n-octanol/water partition coefficient (logP(OW)) are important properties for pharmacology, toxicology and medicinal chemistry. Based on an understanding of the dissolution process, the frontier orbital interaction model was suggested in the present paper to describe the solvent-solut...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms9060962

    authors: Gao S,Cao C

    更新日期:2008-06-01 00:00:00

  • Binding of ochratoxin A to a urinary globulin: a new concept to account for gender difference in rat nephrocarcinogenic responses.

    abstract::SDS-gradient mini-gel electrophoresis and immunoblotting of urine of rats given ochratoxin A (OTA), showed OTA binding to an alpha2u-globulin. Perceived potential internalised delivery of OTA to proximal tubule epithelia by the carrier, specific only to adult male rats and augmenting other uptake mechanisms, suggests ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms9050719

    authors: Mantle PG,Nagy JM

    更新日期:2008-05-01 00:00:00

  • Functional analysis of familial Asp67Glu and Thr1051Ser BRCA1 mutations in breast/ovarian carcinogenesis.

    abstract::Estrogen is believed to be pre-initiator in the risk of breast cancer. The BRCA1 is a tumor suppressor gene associated with breast and ovarian cancer risk. This report describes functional analysis of two BRCA1 missense mutations (Asp67Glu and Thr1051Ser) observed in the familial breast/ovarian cancer patients in Thai...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms10094187

    authors: Pongsavee M,Patmasiriwat P,Saunders GF

    更新日期:2009-09-24 00:00:00

  • A Fish Galectin-8 Possesses Direct Bactericidal Activity.

    abstract::Galectins are a family of animal lectins with high affinity for β-galactosides. Galectins are able to bind to bacteria, and a few mammalian galectins are known to kill the bound bacteria. In fish, no galectins with direct bactericidal effect have been reported. In the present study, we identified and characterized a t...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms22010376

    authors: Zhang T,Jiang S,Sun L

    更新日期:2020-12-31 00:00:00

  • Functional Heterologous Expression of Mature Lipase LipA from Pseudomonas aeruginosa PSA01 in Escherichia coli SHuffle and BL21 (DE3): Effect of the Expression Host on Thermal Stability and Solvent Tolerance of the Enzyme Produced.

    abstract::This study aimed to express heterologously the lipase LipA from Pseudomonas aeruginosa PSA01 obtained from palm fruit residues. In previous approaches, LipA was expressed in Escherichia coli fused with its signal peptide and without its disulfide bond, displaying low activity. We cloned the mature LipA with its trunca...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21113925

    authors: Pulido IY,Prieto E,Pieffet GP,Méndez L,Jiménez-Junca CA

    更新日期:2020-05-30 00:00:00

  • Chrysin Attenuates VCAM-1 Expression and Monocyte Adhesion in Lipopolysaccharide-Stimulated Brain Endothelial Cells by Preventing NF-κB Signaling.

    abstract::Adhesion of leukocytes to endothelial cells plays an important role in neuroinflammation. Therefore, suppression of the expression of adhesion molecules in brain endothelial cells may inhibit neuroinflammation. Chrysin (5,7-dihydroxyflavone) is a flavonoid component of propolis, blue passion flowers, and fruits. In th...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms18071424

    authors: Lee BK,Lee WJ,Jung YS

    更新日期:2017-07-03 00:00:00

  • Chiral Ionic Liquids: Structural Diversity, Properties and Applications in Selected Separation Techniques.

    abstract::Ionic liquids (ILs) are chemical compounds composed of ions with melting points below 100 °C exhibiting a design feature. ILs are commonly used as the so-called green solvents, reagents or highly efficient catalysts in varied chemical processes. The huge application potential of ionic liquids (IL) justifies the growin...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21124253

    authors: Flieger J,Feder-Kubis J,Tatarczak-Michalewska M

    更新日期:2020-06-15 00:00:00

  • HER-2 Expression in Brain Metastases from Colorectal Cancer and Corresponding Primary Tumors: A Case Cohort Series.

    abstract::Brain metastases (BM) from colorectal cancer (CRC) are a rare but increasing event. Surgical resection of oligometastatic disease, including BM, may produce a survival benefit in selected patients. Previous studies described the HER-2 expression patterns in CRC patients, but its prognostic role still remains controver...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms14022370

    authors: Aprile G,De Maglio G,Menis J,Casagrande M,Tuniz F,Pisa EF,Fontanella C,Skrap M,Beltrami AC,Fasola G,Pizzolitto S

    更新日期:2013-01-24 00:00:00

  • Age, Diet and Epidermal Signaling Modulate Dermal Fibroblasts' Adipogenic Potential.

    abstract::The recognition of a distinct fat depot, the dermal white adipose tissue (dWAT), points out the complexity of the interaction among skin resident cells: keratinocytes, dermal fibroblasts (DFs) and adipocytes in response to physiological (diet, age) and pathological (injury) stimulations. dWAT has been recognized as a ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21238955

    authors: Walendzik K,Bukowska J,Kopcewicz M,Machcinska S,Gimble JM,Gawronska-Kozak B

    更新日期:2020-11-25 00:00:00

  • Cyclin-Dependent Kinase Inhibitor Gene TaICK1 acts as a Potential Contributor to Wheat Male Sterility induced by a Chemical Hybridizing Agent.

    abstract::Heterosis has been widely accepted as an effective strategy to increase yields in plant breeding. Notably, the chemical hybridization agent SQ-1 induces male sterility in wheat, representing a critical potential tool in hybrid seed production. However, the mechanisms underlying the male sterility induced by SQ-1 still...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21072468

    authors: Zhang L,Wang C,Yu Y,Zhang Y,Song Y,Li Z,Wang S,Zhang Y,Guo X,Liu D,Li Z,Ma S,Zheng J,Zhao H,Zhang G

    更新日期:2020-04-02 00:00:00

  • Translational Control in p53 Expression: The Role of 5'-Terminal Region of p53 mRNA.

    abstract::In this review, the latest research concerning the structure and function of the 5'-terminal region of p53 mRNA was discussed. Special attention was focused on defined structural motifs which are present in this region, as well as their conservation and plausible functional role in translation. It is known that the le...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20215382

    authors: Swiatkowska A,Dutkiewicz M,Zydowicz-Machtel P,Szpotkowska J,Janecki DM,Ciesiołka J

    更新日期:2019-10-29 00:00:00

  • Meshless method with operator splitting technique for transient nonlinear bioheat transfer in two-dimensional skin tissues.

    abstract::A meshless numerical scheme combining the operator splitting method (OSM), the radial basis function (RBF) interpolation, and the method of fundamental solutions (MFS) is developed for solving transient nonlinear bioheat problems in two-dimensional (2D) skin tissues. In the numerical scheme, the nonlinearity caused by...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms16012001

    authors: Zhang ZW,Wang H,Qin QH

    更新日期:2015-01-16 00:00:00

  • p38 MAPK in Glucose Metabolism of Skeletal Muscle: Beneficial or Harmful?

    abstract::Skeletal muscles respond to environmental and physiological changes by varying their size, fiber type, and metabolic properties. P38 mitogen-activated protein kinase (MAPK) is one of several signaling pathways that drive the metabolic adaptation of skeletal muscle to exercise. p38 MAPK also participates in the develop...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21186480

    authors: Bengal E,Aviram S,Hayek T

    更新日期:2020-09-04 00:00:00

  • The Application of Curve Fitting on the Voltammograms of Various Isoforms of Metallothioneins-Metal Complexes.

    abstract::The translation of metallothioneins (MTs) is one of the defense strategies by which organisms protect themselves from metal-induced toxicity. MTs belong to a family of proteins comprising MT-1, MT-2, MT-3, and MT-4 classes, with multiple isoforms within each class. The main aim of this study was to determine the behav...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms18030610

    authors: Merlos Rodrigo MA,Molina-López J,Jimenez Jimenez AM,Planells Del Pozo E,Adam P,Eckschlager T,Zitka O,Richtera L,Adam V

    更新日期:2017-03-11 00:00:00

  • Clonal expansion of T cells in abdominal aortic aneurysm: a role for doxycycline as drug of choice?

    abstract::Most reported studies with animal models of abdominal aortic aneurysm (AAA) and several studies with patients have suggested that doxycycline favourably modifies AAA; however, a recent large long-term clinical trial found that doxycycline did not limit aneurysm growth. Thus, there is currently no convincing evidence t...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms160511178

    authors: Kroon AM,Taanman JW

    更新日期:2015-05-18 00:00:00

  • Markers of Angiogenesis, Lymphangiogenesis, and Epithelial-Mesenchymal Transition (Plasticity) in CIN and Early Invasive Carcinoma of the Cervix: Exploring Putative Molecular Mechanisms Involved in Early Tumor Invasion.

    abstract::The establishment of a proangiogenic phenotype and epithelial-to-mesenchymal transition (EMT) are considered as critical events that promote the induction of invasive growth in epithelial tumors, and stimulation of lymphangiogenesis is believed to confer the capacity for early dissemination to cancer cells. Recent res...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21186515

    authors: Kurmyshkina O,Kovchur P,Schegoleva L,Volkova T

    更新日期:2020-09-06 00:00:00

  • Coexpression and secretion of endoglucanase and phytase genes in Lactobacillus reuteri.

    abstract::A multifunctional transgenic Lactobacillus with probiotic characteristics and an ability to degrade β-glucan and phytic acid (phytate) was engineered to improve nutrient utilization, increase production performance and decrease digestive diseases in broiler chickens. The Bacillus subtilis WL001 endoglucanase gene (cel...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms150712842

    authors: Wang L,Yang Y,Cai B,Cao P,Yang M,Chen Y

    更新日期:2014-07-21 00:00:00

  • A Microtube Array Membrane (MTAM) Encapsulated Live Fermenting Staphylococcus epidermidis as a Skin Probiotic Patch against Cutibacterium acnes.

    abstract::Antibiotics without selectivity for acne treatment may destroy the beneficial microbes in the human microbiome that helps to fight Cutibacterium acnes (C. acnes), a bacterium associated with inflammatory acne vulgaris. Probiotic treatment by direct application of live Staphylococcus epidermidis (S. epidermidis) onto t...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20010014

    authors: Yang AJ,Marito S,Yang JJ,Keshari S,Chew CH,Chen CC,Huang CM

    更新日期:2018-12-20 00:00:00

  • Small engine, big power: microRNAs as regulators of cardiac diseases and regeneration.

    abstract::Cardiac diseases are the predominant cause of human mortality in the United States and around the world. MicroRNAs (miRNAs) are small non-coding RNAs that have been shown to modulate a wide range of biological functions under various pathophysiological conditions. miRNAs alter target expression by post-transcriptional...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms150915891

    authors: Joladarashi D,Thandavarayan RA,Babu SS,Krishnamurthy P

    更新日期:2014-09-09 00:00:00

  • Carotenoid Cleavage Dioxygenases: Identification, Expression, and Evolutionary Analysis of This Gene Family in Tobacco.

    abstract::Carotenoid cleavage dioxygenases (CCDs) selectively catalyze carotenoids, forming smaller apocarotenoids that are essential for the synthesis of apocarotenoid flavor, aroma volatiles, and phytohormone ABA/SLs, as well as responses to abiotic stresses. Here, 19, 11, and 10 CCD genes were identified in Nicotiana tabacum...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20225796

    authors: Zhou Q,Li Q,Li P,Zhang S,Liu C,Jin J,Cao P,Yang Y

    更新日期:2019-11-18 00:00:00

  • The Mechanism of Melatonin and Its Receptor MT2 Involved in the Development of Bovine Granulosa Cells.

    abstract::Ovarian granulosa cells (GCs) are a critical approach to investigate the mechanism of gene regulation during folliculogenesis. The objective of this study was to investigate the role of MT2 in bovine GCs, and assess whether MT2 silencing affected GCs response to melatonin. We found that MT2 silencing significantly dec...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms19072028

    authors: Wang S,Liu W,Pang X,Dai S,Liu G

    更新日期:2018-07-12 00:00:00

  • Impact and Intricacies of Bone Marrow Microenvironment in B-cell Lymphomas: From Biology to Therapy.

    abstract::Lymphoma, a group of widely prevalent hematological malignancies of lymphocyte origin, has become the focus of significant clinical research due to their high propensity for refractory/relapsed (R/R) disease, leading to poor prognostic outcomes. The complex molecular circuitry in lymphomas, especially in the aggressiv...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21030904

    authors: Sircar A,Chowdhury SM,Hart A,Bell WC,Singh S,Sehgal L,Epperla N

    更新日期:2020-01-30 00:00:00

  • Chromosomal Density of Cancer Up-Regulated Genes, Aberrant Enhancer Activity and Cancer Fitness Genes Are Associated with Transcriptional Cis-Effects of Broad Copy Number Gains in Colorectal Cancer.

    abstract::Broad Copy Number Gains (BCNGs) are copy-number increases of chromosomes or large segments of chromosomal arms. Publicly-available single-nucleotide polymorphism (SNP) array and RNA-Seq data of colon adenocarcinoma (COAD) samples from The Cancer Genome Atlas (TCGA) consortium allowed us to design better control groups...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20184652

    authors: Condorelli DF,Privitera AP,Barresi V

    更新日期:2019-09-19 00:00:00

  • Reevaluation of Pluripotent Cytokine TGF-β3 in Immunity.

    abstract::Transforming growth factor (TGF)-βs are pluripotent cytokines with stimulatory and inhibitory properties for multiple types of immune cells. Analyses of genetic knockouts of each isoform of TGF-β have revealed differing expression patterns and distinct roles for the three mammalian isoforms of TGF-β. Considerable effo...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms19082261

    authors: Komai T,Okamura T,Inoue M,Yamamoto K,Fujio K

    更新日期:2018-08-01 00:00:00

  • The Triple Amino Acid Substitution TAP-IVS in the EPSPS Gene Confers High Glyphosate Resistance to the Superweed Amaranthus hybridus.

    abstract::The introduction of glyphosate-resistant (GR) crops revolutionized weed management; however, the improper use of this technology has selected for a wide range of weeds resistant to glyphosate, referred to as superweeds. We characterized the high glyphosate resistance level of an Amaranthus hybridus population (GRH)-a ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20102396

    authors: García MJ,Palma-Bautista C,Rojano-Delgado AM,Bracamonte E,Portugal J,Alcántara-de la Cruz R,De Prado R

    更新日期:2019-05-15 00:00:00