Abstract：
:Carotenoid cleavage dioxygenases (CCDs) selectively catalyze carotenoids, forming smaller apocarotenoids that are essential for the synthesis of apocarotenoid flavor, aroma volatiles, and phytohormone ABA/SLs, as well as responses to abiotic stresses. Here, 19, 11, and 10 CCD genes were identified in Nicotiana tabacum, Nicotiana tomentosiformis, and Nicotiana sylvestris, respectively. For this family, we systematically analyzed phylogeny, gene structure, conserved motifs, gene duplications, cis-elements, subcellular and chromosomal localization, miRNA-target sites, expression patterns with different treatments, and molecular evolution. CCD genes were classified into two subfamilies and nine groups. Gene structures, motifs, and tertiary structures showed similarities within the same groups. Subcellular localization analysis predicted that CCD family genes are cytoplasmic and plastid-localized, which was confirmed experimentally. Evolutionary analysis showed that purifying selection dominated the evolution of these genes. Meanwhile, seven positive sites were identified on the ancestor branch of the tobacco CCD subfamily. Cis-regulatory elements of the CCD promoters were mainly involved in light-responsiveness, hormone treatment, and physiological stress. Different CCD family genes were predominantly expressed separately in roots, flowers, seeds, and leaves and exhibited divergent expression patterns with different hormones (ABA, MeJA, IAA, SA) and abiotic (drought, cold, heat) stresses. This study provides a comprehensive overview of the NtCCD gene family and a foundation for future functional characterization of individual genes.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Zhou Q,Li Q,Li P,Zhang S,Liu C,Jin J,Cao P,Yang Ydoi
10.3390/ijms20225796subject
Has Abstractpub_date
2019-11-18 00:00:00issue
22issn
1422-0067pii
ijms20225796journal_volume
20pub_type
杂志文章abstract：:The Dunkin Hartley (DH) guinea pig is a widely used naturally occurring osteoarthritis model. The aim of this study was to provide detailed evidence of age-related changes in articular cartilage, subchondral bone mineral density, and estradiol levels. We studied the female Dunkin Hartley guinea pigs at 1, 3, 6, 9, and...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150813578
更新日期：2014-08-05 00:00:00
abstract：:Autophagy is a general protective mechanism for maintaining homeostasis in eukaryotic cells, regulating cellular metabolism, and promoting cell survival by degrading and recycling cellular components under stress conditions. The degradation pathway that is mediated by autophagy receptors is called selective autophagy,...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21062008
更新日期：2020-03-16 00:00:00
abstract：:Melanoma is a lethal tumor because of its severe metastatic potential, and serine/threonine-protein kinase B-raf inhibitors (BRAFi) are used in patients harboring BRAF-mutation. Unfortunately, BRAFi induce resistance. Therefore, we tested the activity of onconase (ONC), a cytotoxic RNase variant, against BRAFi-resista...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20235980
更新日期：2019-11-27 00:00:00
abstract：:Chemotherapy is one of the most common strategies for tumor treatment but often associated with post-therapy tumor recurrence. While chemotherapeutic drugs are known to induce tumor cell senescence, the roles and mechanisms of senescence in tumor recurrence remain unclear. In this study, we used doxorubicin to induce ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22020849
更新日期：2021-01-15 00:00:00
abstract：:Polyhydroxylated dendrimer was synthesized from poly(amidoamine) (PAMAM) dendrimer generation 3 by addition of glycidol (G3gl). G3gl megamer was further modified by binding PAMAM G0 dendrimers by activation of G3gl with p-nitrophenylchloroformate, followed by the addition of excess PAMAM G0 and purification using dial...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20204998
更新日期：2019-10-09 00:00:00
abstract：:Cerebrospinal fluid (CSF) is a readily reachable body fluid that is reflective of the underlying pathological state of the central nervous system (CNS). Hence it has been targeted for biomarker discovery for a variety of neurological disorders. CSF is also the major route for seeding metastases of CNS malignancies and...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms161226150
更新日期：2015-12-07 00:00:00
abstract：:Eggplant varieties rich in bioactive chlorogenic acid along with less browning are preferred by consumers. Therefore, genetics of fruit phenolics, fruit flesh colour, and browning related traits were studied in the genotypes of eggplant, comprising of nine cultivated varieties and one accession of eggplant's primary g...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20122990
更新日期：2019-06-19 00:00:00
abstract：:The relationship between molecularly imprinted polymer (MIP) morphology and template-rebinding over a series of warfarin-imprinted methacrylic acid co(ethylene dimethacrylate) polymers has been explored. Detailed investigations of the nature of template recognition revealed that an optimal template binding was obtain...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14011207
更新日期：2013-01-09 00:00:00
abstract：:Stable five-coordinate Pt(II) complexes have been highlighted as a promising and original platform for the development of new cytotoxic drugs. Their interaction with proteins has been scarcely studied. Here, the reactivity of the five-coordinate Pt(II) compound [Pt(I)(Me) (dmphen)(olefin)] (Me = methyl, dmphen = 2,9-d...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20030520
更新日期：2019-01-26 00:00:00
abstract：:Ricin is one of the most poisonous natural toxins from plants and is classified as a Class B biological threat pathogen by the Centers for Disease Control and Prevention (CDC) of U.S.A. Ricin exposure can occur through oral or aerosol routes. Ricin poisoning has a rapid onset and a short incubation period. There is no...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15057281
更新日期：2014-04-28 00:00:00
abstract：:The melanin-inducing properties of cirsimaritin were investigated in murine B16F10 cells. Cirsimaritin is an active flavone with methoxy groups, which is isolated from the branches of Lithocarpus dealbatus. Tyrosinase activity and melanin content in murine B16F10 melanoma cells were increased by cirsimaritin in a dose...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms16048772
更新日期：2015-04-20 00:00:00
abstract：:Electric field stimulation is known to affect various cellular processes, including cell fate specification and differentiation, particularly towards neuronal lineages. This makes it a promising therapeutic strategy to stimulate regeneration of neuronal tissues. Retinal ganglion cells (RGCs) is a type of neural cells ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20112661
更新日期：2019-05-30 00:00:00
abstract：:The abnormal deposition of calcium within renal parenchyma, termed nephrocalcinosis, frequently occurs as a result of impaired renal calcium handling. It is closely associated with renal stone formation (nephrolithiasis) as elevated urinary calcium levels (hypercalciuria) are a key common pathological feature underlyi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21010369
更新日期：2020-01-06 00:00:00
abstract：:Agarwood is the fragrant resinous material mainly formed from species of Aquilaria. 2-(2-phenylethyl)chromones, especially the highly oxidized 5,6,7,8-tetrahydro-2-(2-phenylethyl)chromones, are the main representative compounds from agarwood. It is important to determine whether agarwood in trade is from cultivated tr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17050771
更新日期：2016-05-23 00:00:00
abstract：:Polymer-based piezoelectric biomaterials have already proven their relevance for tissue engineering applications. Furthermore, the morphology of the scaffolds plays also an important role in cell proliferation and differentiation. The present work reports on poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV), a biocompat...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19082149
更新日期：2018-07-24 00:00:00
abstract：:Endocrine therapy is a key treatment strategy to control or eradicate hormone-responsive breast cancer. However, resistance to endocrine therapy leads to breast cancer relapse. The recent extension of adjuvant tamoxifen treatment up to 10 years actualizes the need for identifying biological markers that may be used to...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms161024243
更新日期：2015-10-14 00:00:00
abstract：:We have previously shown that neural precursor cell-expressed developmentally downregulated gene 4-2 (Nedd4-2) isoforms with a C2 domain are closely related to ubiquitination of epithelial sodium channel (ENaC), resulting in salt-sensitive hypertension by Nedd4-2 C2 targeting in mice. The sodium voltage-gated channel ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18061268
更新日期：2017-06-14 00:00:00
abstract：:In our ongoing research to discover natural products with neuroprotective effects, hyperoside (quercetin 3-O-galactoside) was isolated from Acer tegmentosum, which has been used in Korean traditional medicine to treat liver-related disorders. Here, we demonstrated that hyperoside protects cultured dopaminergic neurons...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20235832
更新日期：2019-11-20 00:00:00
abstract：:We built new hybrid devices consisting of chemical vapor deposition (CVD) grown carbon nanotube (CNT) transistors, decorated with TbPc(2) (Pc = phthalocyanine) rare-earth based single-molecule magnets (SMMs). The drafting was achieved by tailoring supramolecular π-π interactions between CNTs and SMMs. The magnetoresis...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12106656
更新日期：2011-01-01 00:00:00
abstract：:Non-coding RNAs are dominant in the genomic output of the higher organisms being not simply occasional transcripts with idiosyncratic functions, but constituting an extensive regulatory network. Among all the species of non-coding RNAs, small non-coding RNAs (miRNAs, siRNAs and piRNAs) have been shown to be in the cor...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms130810268
更新日期：2012-01-01 00:00:00
abstract：:Growth hormone (GH) plays major anabolic and catabolic roles in the body and is important for regulating several aspects of growth. During an inflammatory process, cells may develop a state of GH resistance during which their response to GH stimulation is limited. In this review, we will emphasize specific mechanisms ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18051019
更新日期：2017-05-09 00:00:00
abstract：:Short tandem repeat (STR) or microsatellite, expansions underlie more than 50 hereditary neurological, neuromuscular and other diseases, including myotonic dystrophy types 1 (DM1) and 2 (DM2). Current disease models for DM1 and DM2 propose a common pathomechanism, whereby the transcription of mutant DMPK (DM1) and CNB...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20133365
更新日期：2019-07-09 00:00:00
abstract：:The growth arrest-specific transcript 5 (GAS5) is a >200-nt lncRNA molecule that regulates several cellular functions, including proliferation, apoptosis, invasion and metastasis, across different types of human cancers. Here, we reviewed the current literature on the expression of GAS5 in leukemia, cervical, breast, ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21207633
更新日期：2020-10-15 00:00:00
abstract：:The pancreas is a key organ involved in digestion and endocrine functions in the body. The major diseases of the pancreas include pancreatitis, pancreatic cancer, cystic diseases, pancreatic divisum, islet cell tumors, endocrine tumors, diabetes mellitus, and pancreatic pain induced by these diseases. While various th...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19113415
更新日期：2018-10-31 00:00:00
abstract：:The increase in antibiotic resistant bacteria demands the development of new antibiotics against preferably new targets. The common approach is to test compounds for their ability to kill bacteria or to design molecules that inhibit essential protein activities in vitro. In the first case, the mode of action of the dr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160817637
更新日期：2015-07-31 00:00:00
abstract：:The unbalanced assignment problem (UAP) is to optimally resolve the problem of assigning n jobs to m individuals (m < n), such that minimum cost or maximum profit obtained. It is a vitally important Non-deterministic Polynomial (NP) complete problem in operation management and applied mathematics, having numerous real...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms161025338
更新日期：2015-10-23 00:00:00
abstract：:Prior evidence indicates that homocysteine plays a role in the development of metabolic syndrome (MetS). Methylenetetrahydrofolate reductase (MTHFR) C677T and methionine synthase reductase (MTRR) A66G polymorphisms are common genetic determinants of homocysteine levels. To investigate the associations of the MTHFR C67...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms151221687
更新日期：2014-11-25 00:00:00
abstract：:Mucin1 (MUC1) is aberrantly glycosylated and overexpressed in various cancers, and it plays a crucial role in cancerogenesis. MUC1 is a type I membranous protein composed of α and β subunits. MUC1-α can be cleaved in cancers, exposing MUC1-β (MUC1-C). MUC1-C is involved with multiple cancer cellular functions, which m...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21093258
更新日期：2020-05-05 00:00:00
abstract：:Demands for safe depigmentation compounds are constantly increasing in the pharmaceutical and cosmetic industry, since the numerous relevant compounds reported to date have shown undesirable side effects or low anti-melanogenic effects. In this study, we reported three novel inhibitors of tyrosinase, which is the key ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21093144
更新日期：2020-04-29 00:00:00
abstract：:Hybrid peptides integrating different functional domains of peptides have many advantages, such as remarkable antimicrobial activity, lower hemolysis and ideal cell selectivity, compared with natural antimicrobial peptides. FV7 (FRIRVRV-NH₂), a consensus amphiphilic sequence was identified as being analogous to host d...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18020339
更新日期：2017-02-06 00:00:00
