Abstract：
:Melanoma is a lethal tumor because of its severe metastatic potential, and serine/threonine-protein kinase B-raf inhibitors (BRAFi) are used in patients harboring BRAF-mutation. Unfortunately, BRAFi induce resistance. Therefore, we tested the activity of onconase (ONC), a cytotoxic RNase variant, against BRAFi-resistant cells to re-establish the efficacy of the chemotherapy. To do so, an A375 dabrafenib-resistant (A375DR) melanoma cell subpopulation was selected and its behavior compared with that of parental (A375P) cells by crystal violet, 5-Bromo-2'-deoxyuridine incorporation, and cleaved poly(ADP-ribose) polymerase 1 (PARP1) western blot measurements. Then, nuclear p65 Nuclear Factor kappaB (NF-κB) and IκB kinases-α/β (IKK) phosphorylation levels were measured. Gelatin zymography was performed to evaluate metalloproteinase 2 (MMP2) activity. In addition, assays to measure migration, invasion and soft agar colony formation were performed to examine the tumor cell dissemination propensity. ONC affected the total viability and the proliferation rate of both A375P and A375DR cell subpopulations in a dose-dependent manner and also induced apoptotic cell death. Among its pleiotropic effects, ONC reduced nuclear p65 NF-κB amount and IKK phosphorylation level, as well as MMP2 activity in both cell subpopulations. ONC decreased cell colony formation, migration, and invasion capability. Notably, it induced apoptosis and inhibited colony formation and invasiveness more extensively in A375DR than in A375P cells. In conclusion, ONC successfully counteracts melanoma malignancy especially in BRAFi-resistant cells and could become a tool against melanoma recurrence.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Raineri A,Fasoli S,Campagnari R,Gotte G,Menegazzi Mdoi
10.3390/ijms20235980subject
Has Abstractpub_date
2019-11-27 00:00:00issue
23issn
1422-0067pii
ijms20235980journal_volume
20pub_type
杂志文章abstract：:Fluoroaromatic compounds are used as agrochemicals and released into environment as pollutants. Glycosylation of 2-, 3-, and 4-fluorophenols using plant cell cultures of Nicotiana tabacum was investigated to elucidate their potential to metabolize these compounds. Cultured N. tabacum cells converted 2-fluorophenol int...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms10051942
更新日期：2009-04-27 00:00:00
abstract：:Although the role of platelet-rich plasma (PRP) in tissue regeneration has been confirmed in many studies, the mechanism of this process is still not fully understood. Human keratinocytes (HaCaT) cells were used as an experimental model for studies on the effects of PRP on cell proliferation, migration, collagen biosy...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22020936
更新日期：2021-01-19 00:00:00
abstract：:The SGNH family esterases are highly effective biocatalysts due to their strong catalytic efficiencies, great stabilities, relatively small sizes, and ease of immobilization. Here, a novel SGNH family esterase (LaSGNH1) from Lactobacillus acidophilus NCFM, which has homologues in many Lactobacillus species, was identi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21010091
更新日期：2019-12-21 00:00:00
abstract：:Indisputably, cancer is a global crisis that requires immediate intervention. Despite the use of conventional treatments over the past decades, it is acceptable to admit that these are expensive, invasive, associated with many side effects and, therefore, a reduced quality of life. One of the most possible solutions t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20194771
更新日期：2019-09-26 00:00:00
abstract：:Awns, which are either bristles or hair-like outgrowths of lemmas in the florets, are one of the typical morphological characteristics of grass species. These stiff structures contribute to grain dispersal and burial and fend off animal predators. However, their phenotypic and genetic associations with traits deciding...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21207593
更新日期：2020-10-14 00:00:00
abstract：:The domestic ferret (Mustela putorius furo) is an important model organism for the study of avian influenza and other diseases of humans and animals, as well as a popular pet animal. In order to evaluate genetic diversity and study disease relationships in ferrets, 22 nuclear microsatellite loci (17 dinucleotide and 5...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms131216592
更新日期：2012-12-05 00:00:00
abstract：:Chitosan is an amino polysaccharide found in nature, which is biodegradable, nontoxic and biocompatible. It has versatile features and can be used in a variety of applications including films, packaging, and also in medical surgery. Recently a possibility to diversify chitosan properties has emerged by combining it wi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12031908
更新日期：2011-01-01 00:00:00
abstract：:Leukemia is the most common cancer in children, representing 30% of all childhood cancers. The disease arises from recurrent genetic insults that block differentiation of hematopoietic stem and/or progenitor cells (HSPCs) and drives uncontrolled proliferation and survival of the differentiation-blocked clone. Pediatri...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms17040461
更新日期：2016-03-29 00:00:00
abstract：:Recently, neuromediators such as substance P (SP) have been found to be important factors in tendon homeostasis. Some studies have found SP to be the cause of inflammation and tendinopathy, whereas others have determined it to be a critical component of tendon healing. As demonstrated by these conflicting findings, th...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21228633
更新日期：2020-11-16 00:00:00
abstract：:This study evaluated the genetic diversity and population structures in a novel cotton germplasm collection comprising 132 diploids, including Glossypium klotzschianum and allotetraploid cotton accessions, including Glossypium barbadense, Glossypium darwinii, Glossypium tomentosum, Glossypium ekmanianum, and Glossypiu...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19082401
更新日期：2018-08-14 00:00:00
abstract：:Hericium Erinaceus (HE) is a medicinal plant known to possess anticarcinogenic, antibiotic, and antioxidant activities. It has been shown to have a protective effect against ischemia-injury-induced neuronal cell death in rats. As an extending study, here we examined in pheochromocytoma 12 (PC12) cells, whether HE coul...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21062138
更新日期：2020-03-20 00:00:00
abstract：:Prediction of microRNA-mRNA interaction typically relies on bioinformatic methods, but these methods only suggest the possibility of microRNA binding and may miss important interactions as well as falsely predict others. A major obstacle to the miRNA research has been the lack of experimental procedures for the identi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150814753
更新日期：2014-08-22 00:00:00
abstract：BACKGROUND:Our aim was to evaluate the feasibility of a serological assessment of gastric cancer risk in patients undergoing colonoscopy in countries with low-to-moderate incidence rates. METHODS:Serum samples were prospectively collected from 453 patients (>50 years old) undergoing colonoscopies. Of these, 279 (61.6%...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19123854
更新日期：2018-12-03 00:00:00
abstract：:At present, due to the growing attention focused on the issue of tendon-bone healing, we carried out an animal study of the use of genetic intervention combined with cell transplantation for the promotion of this process. Here, the efficacy of bone marrow stromal cells infected with bone morphogenetic protein-2 (BMP-2...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms131013605
更新日期：2012-10-22 00:00:00
abstract：:In addition to its role in ribosome biogenesis, ribosomal protein S3 (RPS3), a component of the 40S ribosomal subunit, has been suggested to possess several extraribosomal functions, including an apoptotic function. In this study, we demonstrated that in the mouse brain, the protein levels of RPS3 were altered by the ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19092723
更新日期：2018-09-12 00:00:00
abstract：:Brain-derived neurothrophic factor (BDNF) is a neurotrophin expressed in different tissues and cells, including neurons, endothelial cells, leukocytes, megakaryocytes and platelets. Modifications of BDNF in plasma and/or in serum are associated with neurodegenerative and psychiatric disorders, cardiovascular diseases,...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18091987
更新日期：2017-09-15 00:00:00
abstract：:The transmembrane protein cytochrome c oxidase (CcO) is the terminal oxidase in the respiratory chain of many aerobic organisms and catalyzes the reduction of dioxygen to water. This process maintains an electrochemical proton gradient across the membrane hosting the oxidase. CcO is a well-established model enzyme in ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21196981
更新日期：2020-09-23 00:00:00
abstract：:Environmental issues pose huge threats to public health, particularly the damage caused by fine particulate matter (PM2.5). However, the mechanisms of injury require further investigation and medical materials that can protect the lungs from PM2.5 are needed. We have found that Colla corii asini, a traditional Chinese...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20010055
更新日期：2018-12-23 00:00:00
abstract：:Objectives: Premature birth, defined as less than 37 weeks gestation, affects approximately 12% of all live births around the world. Advances in neonatal care have resulted in the increased survival of infants born prematurely. Although prematurity is a known risk factor for different cardiovascular diseases, little i...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21041305
更新日期：2020-02-14 00:00:00
abstract：:The Dunkin Hartley (DH) guinea pig is a widely used naturally occurring osteoarthritis model. The aim of this study was to provide detailed evidence of age-related changes in articular cartilage, subchondral bone mineral density, and estradiol levels. We studied the female Dunkin Hartley guinea pigs at 1, 3, 6, 9, and...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150813578
更新日期：2014-08-05 00:00:00
abstract：:Na,K-ATPase is an ionic pump that regulates the osmotic equilibrium and membrane potential of cells and also functions as a signal transducer. The interaction of Na,K-ATPase with translationally controlled tumor protein (TCTP) results, among others, in the inhibition of the former's pump activity and in the initiation...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19061657
更新日期：2018-06-04 00:00:00
abstract：:Protein aggregation into insoluble amyloid fibrils is the hallmark of several neurodegenerative diseases, chief among them Alzheimer's and Parkinson's. Although caused by different proteins, these pathologies share some basic molecular mechanisms with familial amyloidotic polyneuropathy (FAP), a rare hereditary neurop...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17091428
更新日期：2016-08-31 00:00:00
abstract：OBJECTIVES:Bladder cancer is a heterogeneous malignancy. Therefore, it is difficult to find single predictive markers. Moreover, most studies focus on either the immunohistochemical or molecular assessment of tumor tissues by next-generation sequencing (NGS) or PCR, while a combination of immunohistochemistry (IHC) and...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21197420
更新日期：2020-10-08 00:00:00
abstract：:SIRT1, an NAD(+)-dependent deacetylase, has been described in the literature as a major player in the regulation of cellular stress responses. Its expression has been shown to be altered in cancer cells, and it targets both histone and non-histone proteins for deacetylation and thereby alters metabolic programs in res...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms16010950
更新日期：2015-01-05 00:00:00
abstract：:Salivary gland dysfunction induces salivary flow reduction and a dry mouth, and commonly involves oral dysfunction, tooth structure deterioration, and infection through reduced salivation. This study aimed to investigate the impact of aging on the salivary gland by a metabolomics approach in an extensive aging mouse m...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22010404
更新日期：2021-01-02 00:00:00
abstract：:Acute lung injury is characterized by the infiltration of neutrophils into lungs and the subsequent impairment of lung function. Here we explored the role of TDAG8 in lung injury induced by lipopolysaccharide (LPS) administrated intratracheally. In this model, cytokines and chemokines released from resident macrophage...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms161226145
更新日期：2015-12-04 00:00:00
abstract：:Actomyosin-mediated contractility is required for the majority of force-driven cellular events such as cell division, adhesion, and migration. Under pathological conditions, the role of actomyosin contractility in malignant phenotypes of various solid tumors has been extensively discussed, but the pathophysiological r...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21103460
更新日期：2020-05-14 00:00:00
abstract：:The interactions of chemokines with their G protein-coupled receptors promote the migration of leukocytes during normal immune function and as a key aspect of the inflammatory response to tissue injury or infection. This review summarizes the major cellular and biochemical mechanisms by which the interactions of chemo...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18020342
更新日期：2017-02-07 00:00:00
abstract：:Drought stress is a major obstacle to agriculture. Although many studies have reported on plant drought tolerance achieved via genetic modification, application of plant growth-promoting rhizobacteria (PGPR) to achieve tolerance has rarely been studied. In this study, the ability of three isolates, including Bacillus ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20246271
更新日期：2019-12-12 00:00:00
abstract：:The application of nanotechnology for the treatment of cancer is mostly based on early tumor detection and diagnosis by nanodevices capable of selective targeting and delivery of chemotherapeutic drugs to the specific tumor site. Due to the remarkable properties of gold nanoparticles, they have long been considered as...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19071979
更新日期：2018-07-06 00:00:00
