Abstract:
:Solid stress is a biomechanical abnormality of the tumor microenvironment that plays a crucial role in tumor progression. When it is applied to cancer cells, solid stress hinders their proliferation rate and promotes cancer cell invasion and metastatic potential. However, the underlying mechanisms of how it is implicated in cancer metastasis is not yet fully understood. Here, we used two pancreatic cancer cell lines and an established in vitro system to study the effect of solid stress-induced signal transduction on pancreatic cancer cell migration as well as the mechanism involved. Our results show that the migratory ability of cells increases as a direct response to solid stress. We also found that Growth Differentiation Factor 15 (GDF15) expression and secretion is strongly upregulated in pancreatic cancer cells in response to mechanical compression. Performing a phosphoprotein screening, we identified that solid stress activates the Akt/CREB1 pathway to transcriptionally regulate GDF15 expression, which eventually promotes pancreatic cancer cell migration. Our results suggest a novel solid stress signal transduction mechanism bringing GDF15 to the centre of pancreatic tumor biology and rendering it a potential target for future anti-metastatic therapeutic innovations.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Kalli M,Minia A,Pliaka V,Fotis C,Alexopoulos LG,Stylianopoulos Tdoi
10.1038/s41598-018-37425-6subject
Has Abstractpub_date
2019-01-30 00:00:00pages
978issue
1issn
2045-2322pii
10.1038/s41598-018-37425-6journal_volume
9pub_type
杂志文章abstract::Chronic inflammation-associated bone destruction, which is observed in rheumatoid arthritis (RA) and periodontitis, is mediated by excessive osteoclastogenesis. We showed previously that γ-glutamyltranspeptidase (GGT), an enzyme involved in glutathione metabolism, acts as an endogenous activator of such pathological o...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35930
更新日期:2016-10-24 00:00:00
abstract::Interleukin 17 (IL-17) stimulates the osteogenic differentiation of progenitor cells in vitro through a synergy with bone morphogenetic protein (BMP)-2. This study investigates whether the diverse responses mediated by IL-17 in vivo also lead to enhanced BMP-2-induced bone formation. Since IL-17 is known to induce ost...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-25564-9
更新日期:2018-05-08 00:00:00
abstract::Parvalbumin-containing projection neurons of the medial-septum-diagonal band of Broca ([Formula: see text]) are essential for hippocampal rhythms and learning operations yet are poorly understood at cellular and synaptic levels. We combined electrophysiological, optogenetic, and modeling approaches to investigate [For...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80245-w
更新日期:2021-01-22 00:00:00
abstract::The strong electronegativity of O dictates that the ground state of singlet CO has positively charged C and negatively charged O, in agreement with ab initio charge analysis, but in disagreement with the dipole direction. Though this unusual phenomenon has been fairly studied, the study of electrostatic potential (EP)...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16307
更新日期:2015-11-06 00:00:00
abstract::A bistable toggle switch is a paradigmatic model in the field of biology. The dynamics of the system induced by Gaussian noise has been intensively investigated, but Gaussian noise cannot incorporate large bursts typically occurring in real experiments. This paper aims to examine effects of variations from one protein...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep31505
更新日期:2016-08-19 00:00:00
abstract::Spliced leader dependent trans-splicing (SLTS) has been described as an important RNA regulatory process that occurs in different organisms, including the trematode Schistosoma mansoni. We identified more than seven thousand putative SLTS sites in the parasite, comprising genes with a wide spectrum of functional class...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-22093-3
更新日期:2018-03-01 00:00:00
abstract::We combine matter-wave interferometry and cavity optomechanics to propose a coherent matter-light interface based on mechanical motion at the quantum level. We demonstrate a mechanism that is able to transfer non-classical features imprinted on the state of a matter-wave system to an optomechanical device, transducing...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03378
更新日期:2013-11-29 00:00:00
abstract::Voltage-gated sodium channels (VGSCs) play a vital role in controlling neuronal excitability. Nav1.6 is the most abundantly expressed VGSCs subtype in the adult central nervous system and has been found to contribute to facilitate the hyperexcitability of neurons after electrical induction of status epilepticus (SE). ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38108
更新日期:2016-12-01 00:00:00
abstract::This present paper was focused on the manufacture of activated carbon (AC) and its application in the electrokinetic remediation (EKR) technology on removal of the heavy metals (HMs) from the municipal solid waste incineration fly ash. AC was produced from Neosinocalamus affinis (NF) by chemical activation with H3PO4 ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39312
更新日期:2016-12-21 00:00:00
abstract::Extracellular matrix (ECM) mechanical properties upregulate cancer invasion, cell contractility, and focal adhesion formation. Alteration in energy metabolism is a known characteristic of cancer cells (i.e., Warburg effect) and modulates cell invasion. There is little evidence to show if collagen density can alter can...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35381-9
更新日期:2018-11-20 00:00:00
abstract::Ring-opening graft polymerization (ROGP) of L-Lactide (L-LA) is a practical method of altering the physical and chemical properties of lignocellulose. Previous studies have mainly investigated cellulose and tin-based catalysts, particularly of tin(II) 2-ethylhexanoate (Sn(oct)2), at high temperatures and reported low ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00464-6
更新日期:2017-04-03 00:00:00
abstract::In this study, we sequenced the complete mitochondrial genome of Eligma narcissus and compared it with 18 other lepidopteran species. The mitochondrial genome (mitogenome) was a circular molecule of 15,376 bp containing 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes and an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26387
更新日期:2016-05-25 00:00:00
abstract::When a rat is on a limited fixed-time food schedule with full access to a running wheel (activity-based anorexia model, ABA), its activity level will increase hours prior to the feeding period. This activity, called food-anticipatory activity (FAA), is a hypothesized parallel to the hyperactivity symptom in human anor...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03929
更新日期:2014-01-29 00:00:00
abstract::Genome editing via CRISPR/Cas9 has become an efficient and reliable way to make precise, targeted changes to the genome of living cells. CXCR4 is a co-receptor for the human immunodeficiency virus type 1 (HIV-1) infection and has been considered as an important therapeutic target for AIDS. CXCR4 mediates viral entry i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15577
更新日期:2015-10-20 00:00:00
abstract::Stochastic differential equations (SDEs) are ubiquitous across disciplines, and uncovering SDEs driving observed time series data is a key scientific challenge. Most previous work on this topic has relied on restrictive assumptions, undermining the generality of these approaches. We present a novel technique to uncove...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-81162-2
更新日期:2021-01-29 00:00:00
abstract::Despite advances in stem cell research, cell transplantation therapy for liver failure is impeded by a shortage of human primary hepatocytes (HPH), along with current differentiation protocol limitations. Several studies have examined the concept of co-culture of human induced pluripotent cells (hiPSCs) with various t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45514-3
更新日期:2019-06-20 00:00:00
abstract::We study the spin-orbit interaction of two-dimensional electron/hole gas (2DEGs/2DHGs) on quasi-2D potassium niobates (KNs) via first-principles calculations. The strong surface polarity changes the free surface states from 2DEGs to 2DHGs. The in-plane dipole maintained on 2D models leads to giant Zeeman-type spin spl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60653-8
更新日期:2020-02-28 00:00:00
abstract::The potential for epigenetic changes in host cells following microbial infection has been widely suggested, but few examples have been reported. We assessed genome-wide patterns of DNA methylation in human macrophage-like U937 cells following infection with Burkholderia pseudomallei, an intracellular bacterial pathoge...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30861
更新日期:2016-08-03 00:00:00
abstract::In our experiment, it was observed that the emission of direct-gap band in germanium with Ge-GeSn layers on one-dimensional (1D) structure. The results of experiment and calculation demonstrate that the uniaxial tensile strain in the (111) and (110) direction can efficiently transform Ge to a direct bandgap material w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24802
更新日期:2016-04-21 00:00:00
abstract::We performed continuous recordings (May 2015 - January 2017) of hydraulic pressure and electrical conductivity of groundwater in the 190 m-long horizontal S13 borehole drilled next to the deep underground laboratories of Gran Sasso (LNGS-INFN), located in the core of the Gran Sasso carbonate aquifer (central Italy) at...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-34444-1
更新日期:2018-10-29 00:00:00
abstract::As a potential alternative to antibiotics, phages can be used to treat multi-drug resistant bacteria. As such, the biological characteristics of phages should be investigated to utilize them as effective antimicrobial agents. In this study, phage PaoP5, a lytic virus that infects Pseudomonas aeruginosa PAO1, was isola...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34067
更新日期:2016-09-23 00:00:00
abstract::The potency of cellular immune responses strongly depends on T cell avidity to antigen. Yet, functional avidity measurements are rarely performed in patients, mainly due to the technical challenges of characterizing heterogeneous T cells. The mean functional T cell avidity can be determined by the IFN-γ Elispot assay,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44320
更新日期:2017-03-13 00:00:00
abstract::The anterior chamber of the eye (ACE) has emerged as a promising clinical islet transplantation site because of its multiple advantages over the conventional intra-hepatic portal site. This includes reduced surgical invasiveness and increased islet graft survival rate. It also allows for enhanced accessibility and mon...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40404-0
更新日期:2019-03-08 00:00:00
abstract::Free carriers, polarons (P), in conjugated polymers play a key role in the performance of optoelectronic devices. Here, we present solid evidence that P can be predominantly generated from polaron pairs (PP) in a poly(3-hexylthiophene) (P3HT) film under zero electric field. P formation from PP strongly depends on temp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08436
更新日期:2015-02-13 00:00:00
abstract::The recent emergence of highly virulent human adenoviruses (HAdVs) with new tissue tropisms underscores the need to determine their ontogeny. Here we report complete high quality genome sequences and analyses for all the previously unsequenced HAdV serotypes (n = 20) within HAdV species D. Analysis of nucleotide seque...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01812
更新日期:2013-01-01 00:00:00
abstract::Glycan-protein lateral interactions have gained increased attention as important modulators of receptor function, by regulating surface residence time and endocytosis of membrane glycoproteins. The pathogen-recognition receptor DC-SIGN is highly expressed at the membrane of antigen-presenting dendritic cells, where it...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07220-w
更新日期:2017-07-27 00:00:00
abstract::Cardiac output during cardiac catheterization is often estimated using the modified Fick method (COFick). In this proof-of-concept, prospective non-randomized study carried out in a single academic healthcare centre, we examined whether replacing COFick in the Gorlin formula with Cheetah-NICOM monitor cardiac output (...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64753-3
更新日期:2020-05-12 00:00:00
abstract::Hepatic stellate cells (HSC) play a key role in the development of chronic liver disease (CLD). Liraglutide, well-established in type 2 diabetes, showed anti-inflammatory and anti-oxidant properties. We evaluated the effects of liraglutide on HSC phenotype and hepatic microvascular function using diverse pre-clinical ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02866-y
更新日期:2017-06-12 00:00:00
abstract::Gamma radiation is a commonly used adjuvant treatment for abdominally localized cancer. Since its therapeutic potential is limited due to gastrointestinal (GI) syndrome, elucidation of the regenerative response following radiation-induced gut injury is needed to develop a preventive treatment. Previously, we showed th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75171-w
更新日期:2020-10-27 00:00:00
abstract::Aberrant restoration of AR activity is linked with prostate tumor growth, therapeutic failures and development of castrate-resistant prostate cancer. Understanding the processes leading to AR-reactivation should provide the foundation for novel avenues of drug discovery. A differential gene expression study was conduc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10068-9
更新日期:2017-08-29 00:00:00