Abstract：
:The potency of cellular immune responses strongly depends on T cell avidity to antigen. Yet, functional avidity measurements are rarely performed in patients, mainly due to the technical challenges of characterizing heterogeneous T cells. The mean functional T cell avidity can be determined by the IFN-γ Elispot assay, with titrated amounts of peptide. Using this assay, we developed a method revealing the heterogeneity of functional avidity, represented by the steepness/hillslope of the peptide titration curve, documented by proof of principle experiments and mathematical modeling. Our data show that not only natural polyclonal CD8 T cell populations from cancer patients, but also monoclonal T cells differ strongly in their heterogeneity of functional avidity. Interestingly, clones and polyclonal cells displayed comparable ranges of heterogeneity. We conclude that besides the mean functional avidity, it is feasible and useful to determine its heterogeneity (hillslope) for characterizing T cell responses in basic research and patient investigation.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Ioannidou K,Baumgaertner P,Gannon PO,Speiser MF,Allard M,Hebeisen M,Rufer N,Speiser DEdoi
10.1038/srep44320subject
Has Abstractpub_date
2017-03-13 00:00:00pages
44320issn
2045-2322pii
srep44320journal_volume
7pub_type
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