Abstract：
:Glycan-protein lateral interactions have gained increased attention as important modulators of receptor function, by regulating surface residence time and endocytosis of membrane glycoproteins. The pathogen-recognition receptor DC-SIGN is highly expressed at the membrane of antigen-presenting dendritic cells, where it is organized in nanoclusters and binds to different viruses, bacteria and fungi. We recently demonstrated that DC-SIGN N-glycans spatially restrict receptor diffusion within the plasma membrane, favoring its internalization through clathrin-coated pits. Here, we investigated the involvement of the N-glycans of DC-SIGN expressing cells on pathogen binding strengthening when interacting with Candida fungal cells by using atomic force microscope (AFM)-assisted single cell-pathogen adhesion measurements. The use of DC-SIGN mutants lacking the N-glycans as well as blocking glycan-mediated lateral interactions strongly impaired cell stiffening during pathogen binding. Our findings demonstrate for the first time the direct involvement of the cell membrane glycans in strengthening cell-pathogen interactions. This study, therefore, puts forward a possible role for the glycocalyx as extracellular cytoskeleton contributing, possibly in connection with the intracellular actin cytoskeleton, to optimize strengthening of cell-pathogen interactions in the presence of mechanical forces.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Te Riet J,Joosten B,Reinieren-Beeren I,Figdor CG,Cambi Adoi
10.1038/s41598-017-07220-wsubject
Has Abstractpub_date
2017-07-27 00:00:00pages
6713issue
1issn
2045-2322pii
10.1038/s41598-017-07220-wjournal_volume
7pub_type
杂志文章abstract：:Campylobacter hyointestinalis is a member of an emerging group of zoonotic Campylobacter spp. that are increasingly identified in both gastric and non-gastric disease in humans. Here, we discovered C. hyointestinalis in three separate classes of New Zealand ruminant livestock; cattle, sheep and deer. To investigate th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20889-x
更新日期：2018-02-05 00:00:00
abstract：:The aesthetic value of landscapes contributes to human well-being. However, studies which have investigated the link between biodiversity and ecosystem services have not taken aesthetic value into account. In this study we evaluated how the aesthetics of coralligenous reefs, a key marine ecosystem in the Mediterranean...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34229
更新日期：2016-09-28 00:00:00
abstract：:p53 and aldehyde dehydrogenase (ALDH) have been implicated in key tumorigenesis processes including cancer initiating cell (CIC) maintenance; however, the relationship between these two mediators remains poorly defined. In this study, ALDH isoform expression diversity was revealed in CICs with disparate p53 functional...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57758-5
更新日期：2020-01-23 00:00:00
abstract：:The alveolar echinococcosis of human is a severe helminthic disease caused by the larva of Echinococcus multilocularis tapeworms. Novel compounds or therapy strategies for the treatment of alveolar echinococcosis are urgently needed due to the limitation of the widely used albendazole. Magnetic microspheres as drug ca...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69484-z
更新日期：2020-07-28 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-63129-x
更新日期：2020-04-16 00:00:00
abstract：:The centrosome is the microtubule organizing center of human cells and facilitates a myriad of cellular functions including organization of the mitotic spindle to ensure faithful chromosome segregation during mitosis, cell polarization and migration, and primary cilia formation. A numerical increase in centrosomes, or...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68629-4
更新日期：2020-07-17 00:00:00
abstract：:Recent studies suggest that glypican-1 (GPC-1) is a biomarker for prostate cancer, but there are few studies elucidating the role of GPC-1 in prostate cancer progression. We observed high expression of GPC-1 in more aggressive prostate cancer cell lines such as PC-3 and DU-145. While inhibition of GPC-1 expression in ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47874-2
更新日期：2019-08-07 00:00:00
abstract：:Ovule-derived haploid culture is an effective and important method for genetic study and plant breeding. Gerbera hybrida is a highly heterozygous species, and the lack of homozygous lines presents a challenge for molecular genetic research. Therefore, we performed haploid induction through unpollinated ovule culture a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-58552-z
更新日期：2020-02-03 00:00:00
abstract：:Despite well-established sex differences for cognition, audition, and somatosensation, few studies have investigated whether there are also sex differences in visual perception. We report the results of fifteen perceptual measures (such as visual acuity, visual backward masking, contrast detection threshold or motion ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-25298-8
更新日期：2018-05-14 00:00:00
abstract：:Translesion synthesis (TLS) is the mechanism by which DNA polymerases replicate through unrepaired DNA lesions. TLS is activated by monoubiquitination of the homotrimeric proliferating cell nuclear antigen (PCNA) at lysine-164, followed by the switch from replicative to specialized polymerases at DNA damage sites. Pol...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15759
更新日期：2015-10-27 00:00:00
abstract：:The Xanthomonadaceae family consists of species of non-pathogenic and pathogenic γ-proteobacteria that infect different hosts, including humans and plants. In this study, we performed a comparative analysis using 69 fully sequenced genomes belonging to this family, with a focus on identifying proteins enriched in phyt...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16325-1
更新日期：2017-11-23 00:00:00
abstract：:Behavioral addiction (BA) is characterized by repeated, impulsive and compulsive seeking of specific behaviors, even with consequent negative outcomes. In drug addiction, alterations in biological mechanisms, such as monoamines and epigenetic processes, have been suggested, whereas whether such mechanisms are also alt...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68741-5
更新日期：2020-07-16 00:00:00
abstract：:Using time-gated fluorescence lifetime imaging microscopy, significantly more signals from 3,6-bis(1-methyl-2-vinyl-pyridinium) carbazole diiodide (o-BMVC) foci, characterized by the longer fluorescent decay time of o-BMVC, were detected in six types of cancer cells than in three types of normal cells. Accumulating ev...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34378-8
更新日期：2018-10-31 00:00:00
abstract：:High-Q microresonator is perceived as a promising platform for optical frequency comb generation, via dissipative soliton formation. In order to achieve a higher quality factor and obtain the necessary anomalous dispersion, multi-mode waveguides were previously implemented in Si3N4 microresonators. However, coupling b...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26255
更新日期：2016-05-16 00:00:00
abstract：:Recent studies have shown that L-proline is a natural osmoprotectant and an antioxidant to protect cells from injuries such as that caused by freezing and thawing in many species including plant, ram sperm and human endothelial cells. Nevertheless, this nontoxic cryoprotectant has not yet been applied to mammalian ooc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26326
更新日期：2016-07-14 00:00:00
abstract：:Developing the high-efficient and green synthetic method for chiral amino alcohols is an intriguing target. We have developed the Mg(2+)-doped Cu/ZnO/Al2O3 catalyst for hydrogenation of L-phenylalanine methyl ester to chiral L-phenylalaninol without racemization. The effect of different L-phenylalanine esters on this ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33196
更新日期：2016-09-13 00:00:00
abstract：:Many insects possess the ability to detect fine fluctuations in the environmental CO2 concentration. In herbivorous species, plant-emitted CO2, in combination with other sensory cues, affect many behaviors including foraging and oviposition. In contrast to the comprehensive knowledge obtained on the insect olfactory p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76918-1
更新日期：2020-11-16 00:00:00
abstract：:Posttraumatic growth (PTG) is defined as positive psychological change in the wake of highly challenging circumstances. Healthcare workers in particular are more vulnerable to stressors and trauma than the general population. The current study examined the use and effectiveness of a novel positive psychological interv...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39189
更新日期：2016-12-20 00:00:00
abstract：:During skin healing, periostin facilitates myofibroblast differentiation through a β1 integrin/FAK dependent mechanism and continued expression is associated with scarring. In contrast to skin, gingival tissue does not typically scar upon injury, but the role of periostin in gingival healing has never been investigate...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35805-6
更新日期：2019-02-25 00:00:00
abstract：:It is essential to understand how the loss of biodiversity impacts both ecosystem function (EF) and multifunctionality (EMF). Previous studies have mostly focused on predicting how species richness (SR) impacts EMF, while the effect of functional diversity (FD) on EMF remains unclear. Specifically, we know little abou...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43475-1
更新日期：2019-05-06 00:00:00
abstract：:The role of orbital differentiation on the emergence of superconductivity in the Fe-based superconductors remains an open question to the scientific community. In this investigation, we employ a suitable microscopic spin probe technique, namely Electron Spin Resonance (ESR), to investigate this issue on selected chemi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06543
更新日期：2014-10-08 00:00:00
abstract：:Few studies have examined the potential transcription factor (TF) simultaneously associated with cisplatin resistance and metastasis in ovarian cancer. To assess a related mechanism, a 345-channel protein/DNA array and transcriptional activity ELISA were performed to compare the TF activities in the cisplatin-sensitiv...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31030-3
更新日期：2018-09-07 00:00:00
abstract：:There is a growing interest in developing microphysiological systems that can be used to model both normal and pathological human organs in vitro. This "organs-on-chips" approach aims to capture key structural and physiological characteristics of the target tissue. Here we describe in vitro vascularized microtumors (V...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31589
更新日期：2016-08-23 00:00:00
abstract：:Genetic linkage maps are critical and indispensable tools in a wide range of genetic and genomic research. With the advancement of genotyping-by-sequencing (GBS) methods, the construction of a high-density and high-resolution linkage maps has become achievable in marine organisms lacking sufficient genomic resources, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14852
更新日期：2015-10-06 00:00:00
abstract：:Bamboo juice is a traditional Chinese drink and herbal medicine, and bamboo juice oral liquids are widely sold for the treatment of cough and phlegm in China. In this study, 26 main compounds of bamboo juice (Phyllostachys edulis) were separated, precisely identified, and qualitative analysis using NMR (nuclear magnet...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76897-3
更新日期：2020-11-12 00:00:00
abstract：:Short chain fatty acids (SCFAs) are protective against inflammatory diseases. Free fatty acid receptor 2 (FFA2), is a target of SCFAs however, their selectivity for FFA2 over other FFA receptors is limited. This study aimed to functionally characterise 2-(4-chlorophenyl)-3-methyl-N-(thiazole-2-yl)butanamide (4CMTB) an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36242-1
更新日期：2018-12-13 00:00:00
abstract：:Transgenic sexing strains (TSS) that carry conditional female lethal genes are advantageous for genetic control programs based on the sterile insect technique (SIT). It is desirable if females die early in development as larval diet is a major cost for mass production facilities. This can be achieved by using a gene p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02763-4
更新日期：2017-05-31 00:00:00
abstract：:In this paper we present a detailed theoretical investigation of the electric field induced mixing effect, in which the up and down frequency-conversion processes are obtained by inducing an effective second order susceptibility via the periodic spatial distribution of reversed biased p-i-n junctions. The possibility ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03485-3
更新日期：2017-06-13 00:00:00
abstract：:Moonlighting proteins, including metabolic enzymes acting as transcription factors (TF), are present in a variety of organisms but have not been described in higher fungi so far. In a previous genome-wide analysis of the TF repertoire of the plant-symbiotic fungus Tuber melanosporum, we identified various enzymes, inc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25165
更新日期：2016-04-28 00:00:00
abstract：:Human milk delivers an array of bioactive components that safeguard infant growth and development and maintain healthy gut microbiota. Milk fat globule membrane (MFGM) is a biologically functional fraction of milk increasingly linked to beneficial outcomes in infants through protection from pathogens, modulation of th...
journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
doi：10.1038/s41598-019-47953-4
更新日期：2019-08-12 00:00:00
