Abstract:
:Ovule-derived haploid culture is an effective and important method for genetic study and plant breeding. Gerbera hybrida is a highly heterozygous species, and the lack of homozygous lines presents a challenge for molecular genetic research. Therefore, we performed haploid induction through unpollinated ovule culture and evaluated the effects of several important factors on this culturing procedure in G. hybrida, including genotype, low temperature, and the development seasons of the ovules. Among 45 G. hybrida cultivars analyzed, 29 cultivars exhibited adventitious bud induction via in vitro unpollinated ovule culture with significant different responses, indicating that the genotype of donor plants was a vital factor for inducibility. Four cultivars with significantly different induction rates, including one non-induced cultivar, were selected to analyze seasonal effects. Ovules extracted in the summer consistently had the highest induction rates, and even the non-induced cultivar included in the analysis could be induced at low levels when ovules from summer were used. Low temperature treatment could also promote adventitious bud induction, and in particular, a strong and significant effect was detected after 7 days of cold treatment. Ploidy level measurements by flow cytometry revealed that 288 ovule-derived regenerants were haploid (55.17%) and 218 lines were diploid (41.76%). Moreover, genetic stability analysis of the regenerants indicated 100% similarity to the marker profile of the mother plant. This is the first report of ovule-derived haploids in G. hybrida, which may facilitate the development of homozygous lines for molecular research and plant breeding.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Li F,Cheng Y,Zhao X,Yu R,Li H,Wang L,Li S,Shan Qdoi
10.1038/s41598-020-58552-zsubject
Has Abstractpub_date
2020-02-03 00:00:00pages
1702issue
1issn
2045-2322pii
10.1038/s41598-020-58552-zjournal_volume
10pub_type
杂志文章abstract::We demonstrate a comprehensive quantitative analysis of vector beam states (VBSs) by using a vector field reconstruction (VFR) technique integrating interferometry and imaging polarimetry, where the analysis is given by a cylindrically polarized Laguerre-Gaussian (LG) mode expansion of VBSs. From test examples of cyli...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46390-7
更新日期:2019-07-10 00:00:00
abstract::Zirconium compounds has been widely attention over the last decades due to its excellent physical and chemical properties. Zirconium nitride nanopowders were synthesized via a simple direct carbothermic nitridation process of internal gel derived zirconia in the presence of nano-sized carbon black. The effects of reac...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55450-x
更新日期:2019-12-16 00:00:00
abstract::Bovine TB (bTB) is endemic in Irish cattle and has eluded eradication despite considerable expenditure, amid debate over the relative roles of badgers and cattle in disease transmission. Using a comprehensive dataset from Northern Ireland (>10,000 km(2); 29,513 cattle herds), we investigated interactions between host ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13062
更新日期:2015-08-17 00:00:00
abstract::CIC-DUX4 sarcoma (CDS) is a group of rare, mesenchymal, small round cell tumours that harbour the unique CIC-DUX4 translocation, which causes aberrant gene expression. CDS exhibits an aggressive course and poor clinical outcome, thus novel therapeutic approaches are needed for CDS treatment. Although patient-derived c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04967-0
更新日期:2017-07-05 00:00:00
abstract::We introduce a new chemical space for drugs and drug-like molecules, exclusively based on their in silico ADME behaviour. This ADME-Space is based on self-organizing map (SOM) applied to 26,000 molecules. Twenty accurate QSPR models, describing important ADME properties, were developed and, successively, used as new m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06692-0
更新日期:2017-07-25 00:00:00
abstract::Ricco's area (the largest area of visual space in which stimulus area and intensity are inversely proportional at threshold) has previously been hypothesised to be a result of centre/surround antagonism in retinal ganglion cell receptive fields, but recent evidence suggests a sizeable cortical contribution. Here, Ricc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21620-6
更新日期:2018-03-01 00:00:00
abstract::Fluorescence-activated cell sorting (FACS) applying flow cytometry to separate cells on a molecular basis is a widespread method. We demonstrate that both fluorescent and unlabeled live cells in a Petri dish observed with a microscope can be automatically recognized by computer vision and picked up by a computer-contr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01088
更新日期:2013-01-01 00:00:00
abstract::The development of new materials with reduced noise and vibration levels is an active area of research due to concerns in various aspects of environmental noise pollution and its effects on health. Excessive vibrations also reduce the service live of the structures and limit the fields of their utilization. In oscilla...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20670-0
更新日期:2018-02-06 00:00:00
abstract::Despite extensive research on various types of meditation, research on the neural correlates of religious chanting is in a nascent stage. Using multi-modal electrophysiological and neuroimaging methods, we illustrate that during religious chanting, the posterior cingulate cortex shows the largest decrease in eigenvect...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40200-w
更新日期:2019-03-12 00:00:00
abstract::Many studies speak in favor of a rhythmic mode of listening, by which the encoding of acoustic information is structured by rhythmic neural processes at the time scale of about 1 to 4 Hz. Indeed, psychophysical data suggest that humans sample acoustic information in extended soundscapes not uniformly, but weigh the ev...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-82008-7
更新日期:2021-01-27 00:00:00
abstract::To determine the incidence of acute cardiac injury (ACI), the factors associated with ACI and the in-hospital mortality in patients with COVID-19, especially in severe patients. All consecutive in-patients with laboratory-confirmed COVID-19 from Tongji Hospital in Wuhan during February 1 and March 29, 2020 were includ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77172-1
更新日期:2020-11-24 00:00:00
abstract::Photodynamic therapy (PDT) is a technique developed to treat the ever-increasing global incidence of cancer. This technique utilises singlet oxygen ((1)O2) generation via a laser excited photosensitiser (PS) to kill cancer cells. However, prolonged sensitivity to intensive light (6-8 weeks for lung cancer), relatively...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03484
更新日期:2013-12-12 00:00:00
abstract::Fluorescent proteins (FP) are used to study various biological processes. Recently, a series of near-infrared (NIR) FPs based on bacterial phytochromes was developed. Finding ways to improve NIR FPs is becoming progressively important. By applying rational design and molecular evolution we have engineered R. palustris...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18348
更新日期:2015-12-18 00:00:00
abstract::Combined treatment with 5-aminolevulinic acid (5-ALA) and X-rays improves tumor suppression in vivo. This is because the accumulated protoporphyrin IX from 5-ALA enhances the generation of ROS by the X-ray irradiation. In the present study, a high-energy medical linear accelerator was used instead of a non-medical low...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21152-z
更新日期:2018-02-09 00:00:00
abstract::MicroRNAs (miRNAs) are small, non-coding RNAs that regulate a variety of biological processes. Recently, human liver-specific miRNA miR-122 has been reported to facilitate hepatitis C virus (HCV) replication in liver cells. HCV is one of the leading causes of liver diseases worldwide. In Pakistan, the estimated preval...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21510
更新日期:2016-02-22 00:00:00
abstract::This paper reports the application of anthracite particles of different sizes and coated with nine kinds of layered double hydroxides (LDHs) varying in M(II)-M(III) cations, as alternative substrates in the simulated vertical-flow constructed wetland columns. Effects of LDHs-coating and particle size of modified anthr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15146
更新日期:2015-10-12 00:00:00
abstract::Telomere DNA ends with a single-stranded 3' overhang. Long 3' overhangs may cause aberrant DNA damage responses and accelerate telomere attrition, which is associated with cancer and aging, respectively. Genetic studies have indicated several important players in preventing 5' end hyper-resection, yet detailed knowled...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08663-x
更新日期:2017-08-18 00:00:00
abstract::We study the tight-binding model for a graphene tube with perimeter N threaded by a magnetic field. We show exactly that this model has different nontrivial topological phases as the flux changes. The winding number, as an indicator of topological quantum phase transition (QPT) fixes at N/3 if N/3 equals to its intege...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep31953
更新日期:2016-08-24 00:00:00
abstract::Neuromuscular impairment requires adherence to a rehabilitation regimen for maximum recovery of motor function. Consumer-grade game controllers have emerged as a viable means to relay supervised physical therapy to patients' homes, thereby increasing their accessibility to healthcare. These controllers allow patients ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59927-y
更新日期:2020-02-28 00:00:00
abstract::Structural cardiac remodeling, accompanying cytoskeletal reorganization of cardiac cells, is a major clinical outcome of diastolic heart failure. A highly local Ca2+ influx across the plasma membrane has been suggested to code signals to induce Rho GTPase-mediated fibrosis, but it is obscure how the heart specifically...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39383
更新日期:2016-12-19 00:00:00
abstract::Landscape's spatial structure has vast implications for the dynamics and distribution of species populations and ecological communities. However, the characterization of the structure of spatial networks has not received nearly as much attention as networks of species interactions counterparts. Recent experiments show...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65669-8
更新日期:2020-05-26 00:00:00
abstract::Contact electrification (CE)-charging of surfaces that are contacted and separated, is a common phenomenon, however it is not completely understood yet. Recent studies using surface imaging techniques and chemical analysis revealed a 'spatial' bipolar distribution of charges at the nano dimension, which made a paradig...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20413-1
更新日期:2018-02-06 00:00:00
abstract::Shewanella species are facultative anaerobic bacteria that colonize redox-stratified habitats where O2 and nutrient concentrations fluctuate. The model species Shewanella oneidensis MR-1 possesses genes coding for three terminal oxidases that can perform O2 respiration: a bd-type quinol oxidase and cytochrome c oxidas...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19726
更新日期:2016-01-27 00:00:00
abstract::In our experiment, it was observed that the emission of direct-gap band in germanium with Ge-GeSn layers on one-dimensional (1D) structure. The results of experiment and calculation demonstrate that the uniaxial tensile strain in the (111) and (110) direction can efficiently transform Ge to a direct bandgap material w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24802
更新日期:2016-04-21 00:00:00
abstract::Type 2 diabetes mellitus (T2DM) is a chronic disease, and dietary modification is a crucial part of disease management. Okara is a sustainable source of fibre-rich food. Most of the valorization research on okara focused more on the physical attributes instead of the possible health attributes. The fermentation of oka...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54021-4
更新日期:2019-11-26 00:00:00
abstract::Mycoplasma pneumoniae (MP) is considered a common cause of pneumonia, causing about 15-20% of adult community-acquired pneumonia (CAP) and up to 40% of cases in children. It has often been observed that MP epidemics last approximately 1-2 years and occur every 3-7 years, with the dominant strains alternating between e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36325-z
更新日期:2019-01-24 00:00:00
abstract::We propose a deep learning model - Probabilistic Prognostic Estimates of Survival in Metastatic Cancer Patients (PPES-Met) for estimating short-term life expectancy (>3 months) of the patients by analyzing free-text clinical notes in the electronic medical record, while maintaining the temporal visit sequence. In a si...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27946-5
更新日期:2018-07-03 00:00:00
abstract::Severe sepsis and septic shock are life-threatening conditions, with Gram-negative organisms responsible for most sepsis mortality. Systemic administration of compounds that block the action of lipopolysaccharide (LPS), a constituent of the Gram-negative outer cell membrane, is hampered by their hydrophobicity and cat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29739
更新日期:2016-07-14 00:00:00
abstract::Clinical practice guides recommend delayed clamping of the umbilical cord. If this is not possible, some authors suggest milking as an alternative. The objective of this study was to determine the variability in professional practice in the management of umbilical cord clamping and milking and to identify factors or c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58641-z
更新日期:2020-02-03 00:00:00
abstract::Untreatable bacterial infections caused by a perpetual increase of antibiotic resistant strains represent a serious threat to human healthcare in the 21(st) century. Conjugative DNA transfer is the most important mechanism for antibiotic resistance and virulence gene dissemination among bacteria and is mediated by a p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24643
更新日期:2016-04-22 00:00:00