Abstract：
BACKGROUND:The aim of the present in vitro study was to explore the possibility of using platelet-rich fibrin (PRF) as a local sustained released device for antibiotics. MATERIALS AND METHODS:Platelet-rich fibrin was prepared with the addition of antibiotics (5 mg/ml metronidazole; 150 mg/ml clindamycin; 1 mU/ml penicillin) or saline prior to centrifugation, while collagen sponges served as control. PRFs anti-bacterial properties were examined in an anti-biogram assay with Staphylococcus aureus or Fusobacterium nucleatum at different time intervals after PRF preparation. RESULTS:The addition of antibiotic solutions at volumes of 2 or 1 ml led to significant changes in PRF's physical properties, while the addition of 0.5 ml solution did not. PRF with saline showed minor anti-bacterial activity, while all PRFs with antibiotics showed significant anti-bacterial activity (p < 0.05). No differences were observed between raw (clot) and pressed (membrane) forms of PRF. Collagen sponges with and without antibiotics showed similar results to PRF. PRF and collagen sponges with antibiotics preserved their anti-bacterial properties 4 days after preparation. CONCLUSIONS:Platelet-rich fibrin incorporated with antibiotics showed long-term anti-bacterial effect against F. nucleatum and S. aureus. This modified PRF preparation may be used to reduce the risk of post-operative infection in addition to the beneficial healing properties of PRF.
journal_name
J Clin Periodontoljournal_title
Journal of clinical periodontologyauthors
Polak D,Clemer-Shamai N,Shapira Ldoi
10.1111/jcpe.13063subject
Has Abstractpub_date
2019-02-01 00:00:00pages
241-247issue
2eissn
0303-6979issn
1600-051Xjournal_volume
46pub_type
杂志文章abstract：OBJECTIVES:This study aimed to investigate the levels of 11 oral species in plaque samples and cytokine levels in biofluid samples of patients with idiopathic uveitis (IU) and systemically healthy individuals (H) with or without gingival inflammation. MATERIAL & METHODS:Twenty-one patients with IU (n = 21), and 22 sys...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/jcpe.12555
更新日期：2016-08-01 00:00:00
abstract：BACKGROUND:The non-communicable diseases dental caries and periodontal diseases pose an enormous burden on mankind. The dental biofilm is a major biological determinant common to the development of both diseases, and they share common risk factors and social determinants, important for their prevention and control. The...
journal_title：Journal of clinical periodontology
pub_type： 共识发展会议,杂志文章,实务指引
doi：10.1111/jcpe.12687
更新日期：2017-03-01 00:00:00
abstract：BACKGROUND:Quaternary ammonium compounds constitute a large group of antibacterial chemicals with a potential for inhibiting plaque and gingivitis. One compound, benzalkonium chloride (BC), may be of value, although there is a dearth of evidence to support efficacy. The aim of this study was to measure the ability of 2...
journal_title：Journal of clinical periodontology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1034/j.1600-051x.2000.027001037.x
更新日期：2000-01-01 00:00:00
abstract：BACKGROUND:Current interest in the assessment of probing attachment level measurements has been stimulated by recent introduction of novel periodontal probes as well as by the reclassification of periodontal diseases. Clinical attachment level (CAL) is currently the "gold standard" for diagnosing and monitoring periodo...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.0303-6979.2004.00464.x
更新日期：2004-03-01 00:00:00
abstract：OBJECTIVE:Investigate the association between the frequency of alcohol consumption and periodontitis. Moreover, evaluate the influence of biological, behavioural, and social risk variables in this association. METHODS:Sample was comprised by 542 subjects of both genders, 35-55 years of age, who underwent a complete pe...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051X.2011.01809.x
更新日期：2012-02-01 00:00:00
abstract：AIMS:In this review, we summarize data on the association between specific periodontal bacterial profiles and tissue gene/protein expression, generated from cell culture models and in vivo studies. MATERIAL AND METHODS:A PubMed search was conducted to identify publications related to the effects of periodontal microbi...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章,评审
doi：10.1111/j.1600-051X.2010.01668.x
更新日期：2011-03-01 00:00:00
abstract：OBJECTIVES:This study examined differences in the efficacy of antibiotics against a single strain of three periodontal pathogens grown in an artificial biofilm. METHODS:Single species biofilms were established with artificial saliva and one of the following bacterial strains: Actinobacillus actinomycetemcomitans Y4, S...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.0303-6979.2004.00490.x
更新日期：2004-05-01 00:00:00
abstract：OBJECTIVES:Several forms of periodontal diseases (PD) are often associated with activated phagocytosing leukocytes and contemporary free radical production. Host antioxidant defenses could benefit from mouthrinses used as adjuncts to counteract plaque-associated bacteria. The aim of the present study was to determine p...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1034/j.1600-051x.2002.290512.x
更新日期：2002-05-01 00:00:00
abstract：AIM:To evaluate the effect of the prebiotic (PREB) mannan oligosaccharide (MOS) on the progression of the experimental periodontitis (EP) and intestinal morphology in rats. MATERIALS AND METHODS:Forty rats were randomly allocated into groups (n = 10): C (control), PREB, EP and EP-PREB. Groups PREB and EP-PREB received...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/jcpe.12987
更新日期：2018-09-01 00:00:00
abstract：AIM:In this study, we investigated the effect of differences in periodontal status in the masticatory performance of dentate subjects with the same occlusal supporting area. MATERIALS AND METHODS:The subjects of the analysis were classified into those of Eichner A1-3 (n = 1094) and Eichner B1-4 (n = 529). Subjects' pe...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/jcpe.12241
更新日期：2014-05-01 00:00:00
abstract：:There is a need for information on the validity of probing depth and attachment level measurements made with automated probes. In this study, 34 teeth in 9 patients were measured with the Florida probe from points marked with a bur prior to extraction. After extraction, connective tissue attachment levels were measure...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1996.tb00573.x
更新日期：1996-05-01 00:00:00
abstract：:Little is known about the relationship of aging to periodontal disease. The immune response undergoes aging-related changes resulting in loss of functional capacity. The aim of this study was to investigate the relationship between levels of serum IgG antibodies against suspected periodontal pathogenic microorganisms ...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1995.tb01782.x
更新日期：1995-11-01 00:00:00
abstract：AIM:The current investigation evaluated changes in levels and proportions of 40 bacterial species in subgingival plaque samples during, immediately after and up to 1 year after metronidazole or amoxicillin therapy combined with SRP. METHOD:After baseline clinical and microbiological monitoring, 17 adult periodontitis ...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1034/j.1600-051x.2001.028007597.x
更新日期：2001-07-01 00:00:00
abstract：AIM:To study transmission of Porphyromonas gingivalis in a population living in a remote area in Southern Java, Indonesia. MATERIAL AND METHODS:Subgingival plaque samples from 167 subjects with varying degrees of periodontal breakdown were obtained and cultured for the presence of P. gingivalis. After extraction and p...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051X.2007.01081.x
更新日期：2007-06-01 00:00:00
abstract：BACKGROUND, AIMS:Zinc-citrate-containing toothpastes have previously been suggested to be of value at inhibiting plaque and gingival inflammation. Present formulations have included triclosan which is thought to contribute major antibacterial/antiplaque effects. Alternative antimicrobial agents and other ingredients su...
journal_title：Journal of clinical periodontology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1034/j.1600-051x.2001.028002157.x
更新日期：2001-02-01 00:00:00
abstract：OBJECTIVES:Safety and efficacy of glycine powder air-polishing (GPAP) in removing subgingival biofilm have been previously demonstrated. The hypothesis that GPAP results in less gingival erosion than sodium bicarbonate air-polishing (SBAP) or hand-instrumentation was assessed. MATERIAL AND METHODS:In each of 10 patien...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章,随机对照试验
doi：10.1111/j.1600-051X.2007.01195.x
更新日期：2008-04-01 00:00:00
abstract：AIM:There is a paucity of up-to-date data regarding prevalence and risk indicators of periodontitis in Italy. Therefore, the aim of this study was to evaluate the prevalence of periodontitis and its risk indicators among adults from an urban area in North Italy. MATERIAL AND METHODS:This cross-sectional survey used a ...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/jcpe.12420
更新日期：2015-07-01 00:00:00
abstract：:The main object of this study was to determine if there was a difference between patients with adult periodontitis and healthy controls in the release of elastase. We also wanted to test the release of alpha-1-antitrypsin and lactoferrin from in vitro-activated peripheral neutrophils. A leukocyte-rich preparation from...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1034/j.1600-051x.1999.260402.x
更新日期：1999-04-01 00:00:00
abstract：:The polymorphonuclear neutrophil (PMN) appears to be an important cell in the protection of the host from pathogenic periodontal micro-organisms. The purpose of the present histological studies was to observe the emigration of the PMN from the gingival vessels to the periodontal pocket during gingivitis and ligature-i...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1985.tb01392.x
更新日期：1985-08-01 00:00:00
abstract：:The aims of the present study were to investigate whether the tachykinins substance P and neurokinin A were present in gingival crevicular fluid in both periodontal health and disease and to study the relationship with periodontal inflammation. Gingival crevicular fluid (GCF) was collected from a healthy, a gingivitis...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1997.tb01192.x
更新日期：1997-11-01 00:00:00
abstract：AIM:the objective of the study was to evaluate the safety and efficacy of a formulation containing recombinant human platelet-derived growth factor (rhPDGF-BB) and β-tricalcium phosphate (β-TCP) in patients with periodontal defects and to compare it with those of β-TCP alone. MATERIALS AND METHODS:in this double-blind...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1111/j.1600-051X.2010.01639.x
更新日期：2011-02-01 00:00:00
abstract：AIM:To identify the molecular mechanisms mediating the persistent defensive functions of the self-renewing junctional epithelium (JE). MATERIALS AND METHODS:Two strains of Wnt reporter mice, Axin2CreErt2/+ ;R26RmTmG/+ and Axin2LacZ/+ , were employed, along with three clinically relevant experimental scenarios where th...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/jcpe.13371
更新日期：2020-12-01 00:00:00
abstract：:One child and one adult with severe periodontitis were investigated for relevant systemic factors and predominant periodontal pocket bacteria. The child had a chronic neutropenia, the adult late yaws, a chronic iron deficiency and possibly rheumatoid arthritis. The predominant organisms in both pocket floras were gram...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1983.tb02202.x
更新日期：1983-03-01 00:00:00
abstract：:This paper presents the combined use of decision trees and cost-effectiveness analysis to determine on which patients new diagnostic tests can be useful, and whether they can change the dentist's decision to provide different services and thus improve patient outcomes in 5 different categories of patients. A decision ...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1996.tb02089.x
更新日期：1996-03-01 00:00:00
abstract：:In four beagle dogs, periodontal tissue breakdown was experimentally induced around the upper left premolars (P1, P2 and P3). Wooden probes were inserted mesially and distally to each premolar. Six different probing forces were used in each dog. Microscopic examination showed: 1) the presence of an intact layer of epi...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1980.tb01975.x
更新日期：1980-08-01 00:00:00
abstract：OBJECTIVE:The aim of the study was to investigate the association between serum lipids and periodontal infection and the role of serum lipids in the association between body weight and periodontal infection. MATERIAL AND METHODS:The Health 2000 Health Examination Survey, which included 8028 subjects aged 30 or older l...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051X.2008.01331.x
更新日期：2008-12-01 00:00:00
abstract：:The present investigation was performed in order to study the reaction of the gingiva in the deciduous and permanent dentition of dogs to 3 weeks of plaque accumulation. The study was carried out in 10 beagles, divided into 2 groups of 5 dogs each; group I and group II. When the dogs of group I were 10 weeks old, a me...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1989.tb01675.x
更新日期：1989-08-01 00:00:00
abstract：:The purpose of the present study was to assess by radiographic means the pattern of destructive periodontal disease within the dentition of individuals of different ages. Full-mouth series of intra-oral radiographs from 531 dentate individuals aged 25-75 years were examined with respect to number and type of remaining...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/j.1600-051x.1988.tb01602.x
更新日期：1988-08-01 00:00:00
abstract：AIM:The aim of this systematic review was to evaluate whether maternal periodontal disease treatment (MPDT) can reduce the incidence of preterm birth (PB) and/or low birth weight (LBW). METHODS:The Cochrane Central Register of Controlled Trials, MEDLINE and EMBASE were searched for entries up to October 2010 without r...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章,meta分析,评审
doi：10.1111/j.1600-051X.2011.01761.x
更新日期：2011-10-01 00:00:00
abstract：AIM:To estimate the prevalence of self-reported gingival bleeding in a representative sample of 12- to 17-year-old Brazilian adolescents. MATERIALS AND METHODS:Sociodemographic and oral health information were obtained through a self-administered questionnaire of the Study of Cardiovascular Risk Factors in Adolescents...
journal_title：Journal of clinical periodontology
pub_type： 杂志文章
doi：10.1111/jcpe.12959
更新日期：2018-08-01 00:00:00
