STRUCTURAL ANALYSIS OF DNA REPAIR PROTEIN XRCC4 APPLYING CIRCULAR DICHROISM IN AN AQUEOUS SOLUTION.

Abstract:

:We applied circular dichroism (CD) spectral analysis to obtain the secondary structure of the DNA repair protein XRCC4, with a focus on its C-terminus. Using synchrotron radiation as a light source across a wide range of wavelengths, including vacuum ultraviolet (UV) light from 180 to 200 nm and UV light from 200 to 240 nm, we determined the secondary structure composition of the full-length protein, including its C-terminus, which had not yet been -the focus of crystallography studies, though it contains several phosphorylation sites. The secondary structures inferred using the obtained CD spectra indicate that the C-terminus is composed of a substantial fraction of turns with a few unordered and alpha-helix structures and almost no beta-strands. The C-terminus is likely to form a characteristic secondary structure with turns as a main component, and its DNA repair function is likely regulated by the structural change induced by phosphorylation of the terminus.

journal_name

Radiat Prot Dosimetry

authors

Nishikubo K,Izumi Y,Matsumoto Y,Fujii K,Matsuo K,Yokoya A

doi

10.1093/rpd/ncy275

subject

Has Abstract

pub_date

2019-05-01 00:00:00

pages

36-39

issue

1-2

eissn

0144-8420

issn

1742-3406

pii

5250488

journal_volume

183

pub_type

杂志文章
  • The history of radon from a Swedish perspective.

    abstract::Beginning in the 16th century, what was later found to be radon was thought to be causing sickness among miners. During the first decades of the 20th century, exposure to radon was seen as being healthy. During the 1940s, 1950s and 1960s researchers thought that the gamma radiation in residences could produce genetic ...

    journal_title:Radiation protection dosimetry

    pub_type: 历史文章,杂志文章,评审

    doi:10.1093/rpd/nch318

    authors: Swedjemark GA

    更新日期:2004-01-01 00:00:00

  • Narrow beam dosimetry for high energy hadrons and electrons.

    abstract::Organ doses and effective dose were calculated with the latest version of the Monte Carlo transport code FLUKA in the case of an anthropomorphic mathematical model exposed to monoenergetic narrow beams of protons, pions and electrons in the energy range 10-400 GeV. The target organs considered were right eye, thyroid,...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.rpd.a006556

    authors: Pelliccioni M,Silari M,Ulrici L

    更新日期:2001-01-01 00:00:00

  • Criteria and suspension levels in diagnostic radiology.

    abstract::The EC (European Council) Directive on radiation protection of patients requires that criteria for acceptability of equipment in diagnostic radiology, nuclear medicine and radiotherapy be established throughout the member states. This study reviews the background to this requirement and to its implementation in practi...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章,评审

    doi:10.1093/rpd/ncs295

    authors: Malone J,Baldelli P,Balter S,Bischof N,Bosmans H,Dowling A,Edyvean S,Gallagher A,Faulkner K,Horner K,Malone L,Mclean ID,O'Connor U,Schreiner A,Vassileva J,Vano E,Zoetelief J

    更新日期:2013-02-01 00:00:00

  • Radiation protection at Hadron therapy facilities.

    abstract::The Italian National Centre for Oncological Hadrontherapy is currently under construction in Pavia. It is designed for the treatment of deep-seated tumours (up to a depth of 27 cm of water equivalent) with proton and C-ion beams as well as for both clinical and radiobiological research. The particles will be accelerat...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/ncr242

    authors: Pelliccioni M

    更新日期:2011-07-01 00:00:00

  • W values of protons in liquid water.

    abstract::The W values of protons in liquid water were calculated for energies from 0.1 keV to 10 MeV using the continuous slowing down approximation as well as three models for the calculation of the differential ionisation cross-sections of water for protons published in recent years. The W values determined by means of the t...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/ncm019

    authors: Baek WY,Grosswendt B

    更新日期:2007-01-01 00:00:00

  • Variability of radiocaesium inventory in Fukushima soil cores from one site measured at different times.

    abstract::The Fukushima Daiichi Nuclear Power Plant (FDNPP) accident has resulted in the deposition of radioactive contamination of soils mainly by (134)Cs and (137)Cs. The contaminated areas are of a great concern since external radiation may lead to significant long-term doses to humans and animals. External dose rate above g...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/ncv276

    authors: Mishra S,Sahoo SK,Arae H,Sorimachi A,Hosoda M,Tokonami S,Ishikawa T

    更新日期:2015-11-01 00:00:00

  • Comparison of effective doses from various monoenergetic particles based on the stylised and the VIP-Man tomographic models.

    abstract::This study compares the effective doses from a MIRD-type stylised model with those derived from the scaled-down version of the tomographic VIP-Man model for photon, electron, neutron and proton beams. The effective dose results from these two models show that they differ from each other within approximately 10% for co...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/nci116

    authors: Xu XG,Chao TC,Bozkurt A

    更新日期:2005-01-01 00:00:00

  • A SIMPLIFIED METHOD FOR THE WATER-EQUIVALENT DIAMETER CALCULATION TO ESTIMATE PATIENT DOSE IN CT EXAMINATIONS.

    abstract::We proposed and evaluated a water-equivalent diameter calculation without using a region of interest (ROI), (Dw,t) and compared it with the results of using a ROI fitted to the patient border (Dw,f). Evaluations were carried out on thoracic and head CT images. We found that the difference between Dw,t and Dw,f was wit...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/ncy214

    authors: Anam C,Arif I,Haryanto F,Widita R,Lestari FP,Adi K,Dougherty G

    更新日期:2019-11-30 00:00:00

  • Patient dose in neonatal units.

    abstract::Lung disease represents one of the most life-threatening conditions in prematurely born children. In the evaluation of the neonatal chest, the primary and most important diagnostic study is therefore the chest radiograph. Since prematurely born children are very sensitive to radiation, those radiographs may lead to a ...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/ncn237

    authors: Smans K,Struelens L,Smet M,Bosmans H,Vanhavere F

    更新日期:2008-01-01 00:00:00

  • Digital dual-parameter data acquisition for SP2 hydrogen-filled proportional counters.

    abstract::Hydrogen-filled proportional counters perform well as neutron spectrometers in the energy region from a few tens of keV up to ∼1.5 MeV. Unfortunately, gamma rays also generate signals in these detectors. It is possible in principle to distinguish the two types of event via the rise time of their respective signal puls...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/nct262

    authors: Hawkes NP,Roberts NJ

    更新日期:2014-10-01 00:00:00

  • Specific features of the influence of high-energy electron beams on the luminescent properties of undoped and Nb, Fe-doped Al₂O₃ crystals.

    abstract::The influence of 10 MeV high-current electron beams accelerated by the M-30 microtron on the luminescent properties of the α-Al₂O₃, Al₂O₃:Nb and Al₂O₃:Fe crystals has been studied. The effect of the long-term phosphorescence at room temperature has been found that can be used to monitor electron and gamma accelerator ...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/ncu213

    authors: Maslyuk VT,Megela IG,Okunieva TO,Pekar JM,Pekar VJ

    更新日期:2014-11-01 00:00:00

  • Local diagnostic reference levels in standard X-ray examinations.

    abstract::The national diagnostic reference levels (NDRLs) form an efficient, concise and powerful standard for optimising radiation protection of a patient. However, in a large hospital, where many radiological departments are present, it is also possible to calculate and define lower dose values as local diagnostic reference ...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/nch432

    authors: Compagnone G,Pagan L,Bergamini C

    更新日期:2005-01-01 00:00:00

  • Minimising activity and dose with enhanced image quality by radiopharmaceutical administrations.

    abstract::Owing to the introduction of new diagnostic procedures, such as computed tomography (CT), positron emission tomography (PET) and single photon emission computed tomography (SPECT), the individual dose caused by medical exposures has grown rapidly in the last years. This is especially a subject to radiation protection ...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/ncq104

    authors: Hoeschen C,Mattsson S,Cantone MC,Mikuz M,Lacasta C,Ebel G,Clinthorne N,Giussani A

    更新日期:2010-04-01 00:00:00

  • Looking into future: challenges in radiation protection in medicine.

    abstract::Radiation protection in medicine is becoming more and more important with increasing wider use of X-rays, documentation of effects besides the potential for long-term carcinogenic effects. With computed tomography (CT) likely to become sub-mSv in coming years, positron emission tomography (PET), single photon emission...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/ncv071

    authors: Rehani MM

    更新日期:2015-07-01 00:00:00

  • Radon in private drinking water wells.

    abstract::At least 10% of inhabitants in the Czech Republic are supplied with water from private sources (private wells, boreholes). With the increasing cost of water, the number of people using their own sources of drinking water will be likely to increase. According to the Decree of the State Office for Nuclear Safety about t...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/ncu095

    authors: Otahal P,Merta J,Burian I

    更新日期:2014-07-01 00:00:00

  • CAPABILITIES OF THE ARADOS-WG03 REGIONAL NETWORK FOR LARGE-SCALE RADIOLOGICAL AND NUCLEAR EMERGENCY SITUATIONS IN ASIA.

    abstract::In 2015, the Asian Radiation Dosimetry Group established a regional network of biological dosimetry laboratories known as the ARADOS-WG03 (Working Group 03; Biological Dosimetry). A survey was conducted in 2017 to evaluate the capabilities and capacities of the participating laboratories for emergency preparedness and...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/ncy279

    authors: Jang S,Suto Y,Liu J,Liu Q,Zuo Y,Duy PN,Miura T,Abe Y,Hamasaki K,Suzuki K,Kodama S

    更新日期:2019-12-31 00:00:00

  • Correlation of phase transition with the change in TL characteristics in CaSO4:Dy phosphor--effect of thermal treatment.

    abstract::The study is aimed at optimising the glow curve structure of CaSO4:Dy phosphor for dosimetric purpose and also to understand the observed changes owing to thermal treatment in the thermoluminescence (TL) sensitivity and glow curve structure. The reversible changes in the intensities of the lower temperature satellite ...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/nci561

    authors: Bakshi AK,Pradhan AS,Tyagi AK,Kher RK,Bhatt BC

    更新日期:2006-01-01 00:00:00

  • Biodosimetry using chromosome aberrations in human lymphocytes.

    abstract::In vitro dose-response calibration curves for (60)Co gamma rays have been established for unstable chromosome aberrations in human peripheral blood lymphocytes. The observed dose-response data were fitted to a linear quadratic model. The calibration curve parameters were used to estimate the equivalent whole-body dose...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/ncl109

    authors: Senthamizhchelvan S,Pant GS,Rath GK,Julka PK,Nair O,Joshi RC,Malhotra A,Pandey RM

    更新日期:2007-01-01 00:00:00

  • A large-scale measurement, analysis and modelling of electromagnetic radiation levels in the vicinity of GSM/UMTS base stations in an urban area.

    abstract::The present study analyses the electric field radiating from the GSM/UMTS base stations located in central Malatya, a densely populated urban area in Turkey. The authors have conducted both instant and continuous measurements of high-frequency electromagnetic fields throughout their research by using non-ionising radi...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/ncv008

    authors: Karadağ T,Yüceer M,Abbasov T

    更新日期:2016-01-01 00:00:00

  • RADIATION DOSE IS OF LIMITED CLINICAL USEFULNESS IN PERSONS WITH ACUTE RADIATION SYNDROME.

    abstract::The relation of radiation exposure (dose) with acute radiation syndrome (ARS) depends on many factors. In this overview, we reconsider (1) radiation exposure characteristics (e.g. radiation quality, fractionation, dose rate, partial/total body irradiation) and (2) biological processes (e.g. radiosensitivity, cell cycl...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/ncz058

    authors: Port M,Majewski M,Abend M

    更新日期:2019-12-31 00:00:00

  • AMANDE: a new facility for monoenergetic neutron fields production between 2 keV and 20 MeV.

    abstract::The variation of the response of the instruments with the neutron energy has to be determined in well-characterized monoenergetic neutron fields. The AMANDE facility will deliver such neutron fields between 2 keV and 20 MeV in an experimental hall designed with metallic walls for neutron scattering minimisation. The n...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/nch185

    authors: Gressier V,Guerre-Chaley JF,Lacoste V,Lebreton L,Pelcot G,Pochat JL,Bolognese-Milstajn T,Champion D

    更新日期:2004-01-01 00:00:00

  • Magnetic field tracking with MCNP5.

    abstract::With the introduction of continuous-energy heavy charged particle transport in MCNP5, the need for tracking charged particles in a magnetic field becomes increasingly important. Two methods for including magnetic field effects on charged particles are included in the proton transport version of the code. The first tec...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/nci048

    authors: Bul JS,Hughes HG,Walstrom PL,Zumbro JD,Mokhov NV

    更新日期:2005-01-01 00:00:00

  • Occurrence of natural radioactivity in public water supplies in Germany: (238)U, (234)U, (235)U, (228)RA, (226)RA, (222)RN, (210)PB, (210)PO and gross alpha activity concentrations.

    abstract::The Federal Office for Radiation Protection performed a representative survey on the radiological quality of drinking water in Germany. The aim of this study was to determine regional variations of natural radionuclide concentrations and to estimate radiation exposures caused by drinking water consumption. The study i...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/ncq139

    authors: Beyermann M,Bünger T,Schmidt K,Obrikat D

    更新日期:2010-09-01 00:00:00

  • Protection from radon exposure at home and at work in the directive 2013/59/Euratom.

    abstract::In recent years, international organisations involved in radiation protection and public health have produced new guidance, recommendations and requirements aiming better protection from radon exposure. These organisations have often worked in close collaboration in order to facilitate the establishment of harmonised ...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/ncu101

    authors: Bochicchio F

    更新日期:2014-07-01 00:00:00

  • Relative implications of protective responses versus damage induction at low dose and low-dose-rate exposures, using the microdose approach.

    abstract::In reviewing the tissue effects of low-dose radiation (1) absorbed dose to tissue is replaced by the sum of energy deposited with track events in cell-equivalent tissue micromasses, i.e. with microdose hits, in the number of exposed micromasses and (2) induced cell damage and adaptive protection are related to microdo...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.rpd.a006197

    authors: Feinendegen LE

    更新日期:2003-01-01 00:00:00

  • Simultaneous Measurements of Nanoaerosols and Radioactive Aerosols Containing the Short-lived Radon Isotopes.

    abstract::The activity size distribution of the Equilibrium-Equivalent Concentration (EER) of 222Rn is one of the most important parameters for the estimation of radiation dose by inhalation of radon decay products. A series of measurements of the EER activity size distribution were performed by the screen diffusion battery in ...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/ncx157

    authors: Otahal PPS,Burian I,Ondracek J,Zdimal V,Holub RF

    更新日期:2017-11-01 00:00:00

  • THE DECADE OF THE RABiT (2005-15).

    abstract::The RABiT (Rapid Automated Biodosimetry Tool) is a dedicated Robotic platform for the automation of cytogenetics-based biodosimetry assays. The RABiT was developed to fulfill the critical requirement for triage following a mass radiological or nuclear event. Starting from well-characterized and accepted assays we deve...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/ncw172

    authors: Garty G,Turner HC,Salerno A,Bertucci A,Zhang J,Chen Y,Dutta A,Sharma P,Bian D,Taveras M,Wang H,Bhatla A,Balajee A,Bigelow AW,Repin M,Lyulko OV,Simaan N,Yao YL,Brenner DJ

    更新日期:2016-12-01 00:00:00

  • IMPORTANCE OF ENGAGING IN DIALOGUE WITH THE POPULATION AFTER A NUCLEAR ACCIDENT.

    abstract::Human behaviour is primarily driven by perceptions and this is particularly important in the aftermath of a nuclear accident. One of the main lessons we can draw from the Chernobyl and Fukushima accidents is that once the acute phase of the accident is over, it is important to engage in dialogue with the affected popu...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/ncw320

    authors: Vanmarcke H

    更新日期:2017-04-01 00:00:00

  • Radiation doses potentially received on-board aeroplanes during recent solar particle events.

    abstract::Because the doses received on-board aeroplanes are now monitored to fulfil legal requirements in some countries, including the European Community, the models to calculate doses received during solar events have left their purely academic status to become a part of operational systems as well. The present work consider...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/nci356

    authors: Lantos P

    更新日期:2006-01-01 00:00:00

  • Bayesian approach for quantifying the uncertainty of neutron doses derived from spectrometric measurements.

    abstract::Bayesian methods provide a unified framework for combining information in the presence of uncertainty. All uncertainties that enter into the description of a measurement are modelled using probability distributions, and these are handled according to the rules of probability theory, ensuring that the approach is free ...

    journal_title:Radiation protection dosimetry

    pub_type: 杂志文章

    doi:10.1093/rpd/ncl096

    authors: Reginatto M

    更新日期:2006-01-01 00:00:00