Abstract:
:Nanostructured materials premise to revolutionize the label-free biosensing of analytes for clinical applications, leveraging the deeper interaction between materials and analytes with comparable size. However, when the characteristic dimension of the materials reduces to the nanoscale, the surface functionalization for the binding of bioreceptors becomes a complex issue that can affect the performance of label-free biosensors. Here we report on an effective and robust route for surface biofunctionalization of nanostructured materials based on the layer-by-layer (LbL) electrostatic nano-assembly of oppositely-charged polyelectrolytes, which are engineered with bioreceptors to enable label-free detection of target analytes. LbL biofunctionalization is demonstrated using nanostructured porous silicon (PSi) interferometers for affinity detection of streptavidin in saliva, through LbL nano-assembly of a bi-layer of positively-charged poly(allylamine hydrochloride) (PAH) and negatively-charged biotinylated poly(methacrylic acid) (b-PMAA). High sensitivity in streptavidin detection is achieved, with high selectivity and stability, down to a detection limit of 600 fM.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Mariani S,Robbiano V,Strambini LM,Debrassi A,Egri G,Dähne L,Barillaro Gdoi
10.1038/s41467-018-07723-8subject
Has Abstractpub_date
2018-12-10 00:00:00pages
5256issue
1issn
2041-1723pii
10.1038/s41467-018-07723-8journal_volume
9pub_type
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