Abstract:
:Characterizing the long-term nanometer-scale interactions between lysosomes and mitochondria in live cells is essential for understanding their functions but remains challenging due to limitations of the existing fluorescent probes. Here, we develop cell-permeable organic fluorescent probes for lysosomes with excellent specificity and high photostability. We also use an existing Atto 647N dye with high brightness and excellent photostability to achieve specific labeling of mitochondria in live cells. Using these probes, we obtain dual-color structured illumination microscopy (SIM) images of dynamic physical lysosome-mitochondrion interactions in live cells at an ~90-nm resolution over a long time course of ~13 min. We successfully record the consecutive dynamic processes of lysosomal fusion and fission, as well as four types of physical lysosome-mitochondrion interactions by super-resolution imaging. Our probes provide an avenue for understanding the functions and the dynamic interplay of lysosomes and mitochondria in live cells.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Han Y,Li M,Qiu F,Zhang M,Zhang YHdoi
10.1038/s41467-017-01503-6subject
Has Abstractpub_date
2017-11-03 00:00:00pages
1307issue
1issn
2041-1723pii
10.1038/s41467-017-01503-6journal_volume
8pub_type
杂志文章abstract::Engineering strong metal-support interactions (SMSI) is an effective strategy for tuning structures and performances of supported metal catalysts but induces poor exposure of active sites. Here, we demonstrate a strong metal-support interaction via a reverse route (SMSIR) by starting from the final morphology of SMSI ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-16674-y
更新日期:2020-06-16 00:00:00
abstract::The metazoan TREX complex is recruited to mRNA during nuclear RNA processing and functions in exporting mRNA to the cytoplasm. Nxf1 is an mRNA export receptor, which binds processed mRNA and transports it through the nuclear pore complex. At present, the relationship between TREX and Nxf1 is not understood. Here we sh...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms2005
更新日期:2012-01-01 00:00:00
abstract::Increasing aridity and drought severity forecast for many land areas could reduce the land carbon (C) sink. However, with limited long-term direct measures, it is difficult to distinguish direct drying effects from counter effects of CO2 enrichment and nitrogen (N) deposition. Here, we document a >50% decline in produ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms8148
更新日期:2015-05-14 00:00:00
abstract::Resident tissue macrophages (RTM) can fulfill various tasks during development, homeostasis, inflammation and repair. In the lung, non-alveolar RTM, called interstitial macrophages (IM), importantly contribute to tissue homeostasis but remain little characterized. Here we show, using single-cell RNA-sequencing (scRNA-...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-11843-0
更新日期:2019-09-03 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature communications
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41467-020-17694-4
更新日期:2020-07-23 00:00:00
abstract::Diffuse large B-cell lymphoma (DLBCL) is an aggressive cancer originating from mature B-cells. Prognosis is strongly associated with molecular subgroup, although the driver mutations that distinguish the two main subgroups remain poorly defined. Through an integrative analysis of whole genomes, exomes, and transcripto...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-06354-3
更新日期:2018-10-01 00:00:00
abstract::Plasma and tumor caveolin-1 (Cav-1) are linked with disease progression in prostate cancer. Here we report that metabolomic profiling of longitudinal plasmas from a prospective cohort of 491 active surveillance (AS) participants indicates prominent elevations in plasma sphingolipids in AS progressors that, together wi...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17645-z
更新日期:2020-08-27 00:00:00
abstract::The core of a ferromagnetic vortex domain creates a strong, localized magnetic field, which can be manipulated on nanosecond timescales, providing a platform for addressing and controlling individual nitrogen-vacancy centre spins in diamond at room temperature, with nanometre-scale resolution. Here, we show that the f...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11584
更新日期:2016-06-14 00:00:00
abstract::Simultaneous, enantiomer-specific identification of chiral molecules in multi-component mixtures is extremely challenging. Many established techniques for single-component analysis fail to provide selectivity in multi-component mixtures and lack sensitivity for dilute samples. Here we show how enantiomers may be diffe...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms8511
更新日期:2015-06-24 00:00:00
abstract::The recently discovered histone post-translational modification crotonylation connects cellular metabolism to gene regulation. Its regulation and tissue-specific functions are poorly understood. We characterize histone crotonylation in intestinal epithelia and find that histone H3 crotonylation at lysine 18 is a surpr...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-02651-5
更新日期:2018-01-09 00:00:00
abstract::Rather than targeting tumour cells directly, elements of the tumour microenvironment can be modulated to sensitize tumours to the effects of therapy. Here we report a unique mechanism by which ectopic microRNA-103 can manipulate tumour-associated endothelial cells to enhance tumour cell death. Using gain-and-loss of f...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms13597
更新日期:2016-11-25 00:00:00
abstract::Nuclear magnetic resonance (NMR) spectroscopy is a powerful method for analyzing the chemical composition and molecular structure of materials. At the nanometer scale, NMR has the prospect of mapping the atomic-scale structure of individual molecules, provided a method that can sensitively detect single nuclei and mea...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-07121-0
更新日期:2018-11-08 00:00:00
abstract::To date, the vast preponderance of somatic variants observed in the cancer genome have been rare variants, and it is common in practice to encounter in a new tumor variants that have not been observed previously. Here we focus on probability estimation for encountering such hitherto unseen variants. We draw upon stati...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-13402-z
更新日期:2019-12-03 00:00:00
abstract::Mangrove forests hold some of the highest densities of carbon recorded in any ecosystem, but have experienced widespread deforestation through conversion to aquaculture and agriculture. Alongside deforestation, mangroves have shown simultaneous natural expansion in some parts of the world, and considerable investments...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18118-z
更新日期:2020-08-26 00:00:00
abstract::Members of the transient receptor potential (TRP) ion channels conduct cations into cells. They mediate functions ranging from neuronally mediated hot and cold sensation to intracellular organellar and primary ciliary signaling. Here we report a cryo-electron microscopy (cryo-EM) structure of TRPC4 in its unliganded (...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-05247-9
更新日期:2018-08-06 00:00:00
abstract::Liquid crystalline phases of matter permeate nature and technology, with examples ranging from cell membranes to liquid-crystal displays. Remarkably, electronic liquid-crystal phases can exist in two-dimensional electron systems (2DES) at half Landau-level filling in the quantum Hall regime. Theory has predicted the e...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-01810-y
更新日期:2017-11-16 00:00:00
abstract::A fundamental puzzle of human evolution is how we evolved to cooperate with genetically unrelated strangers in transient interactions. Group-level selection on culturally differentiated populations is one proposed explanation. We evaluate a central untested prediction of Cultural Group Selection theory, by assessing w...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-14416-8
更新日期:2020-02-04 00:00:00
abstract::Normal epithelial cells are stably connected to each other via the apical junctional complex (AJC). AJCs, however, tend to be disrupted during tumor progression, and this process is implicated in cancer dissemination. Here, using colon carcinoma cells that fail to form AJCs, we investigated molecular defects behind th...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-01945-y
更新日期:2017-11-28 00:00:00
abstract::Sensory perception modulates health and aging across taxa. Understanding the nature of relevant cues and the mechanisms underlying their action may lead to novel interventions that improve the length and quality of life. We found that in the vinegar fly, Drosophila melanogaster, exposure to dead conspecifics in the en...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-10285-y
更新日期:2019-05-30 00:00:00
abstract::The reconstruction of human-driven, Earth-shaping dynamics is important for understanding past human/environment interactions and for helping human societies that currently face global changes. However, it is often challenging to distinguish the effects of the climate from human activities on environmental changes. He...
journal_title:Nature communications
pub_type: 历史文章,杂志文章
doi:10.1038/ncomms4211
更新日期:2014-01-01 00:00:00
abstract::Crosstalk between inflammatory signalling pathways and receptor tyrosine kinases has been revealed as an indicator of cancer malignant progression. In the present study, we focus on EphA2 receptor tyrosine kinase, which is overexpressed in many human cancers. It has been reported that ligand-independent phosphorylatio...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms8679
更新日期:2015-07-09 00:00:00
abstract::Electric eels (Electrophorus electricus) are legendary for their ability to incapacitate fish, humans, and horses with hundreds of volts of electricity. The function of this output as a weapon has been obvious for centuries but its potential role for electroreception has been overlooked. Here it is shown that electric...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms9638
更新日期:2015-10-20 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature communications
pub_type: 已发布勘误
doi:10.1038/s41467-019-13141-1
更新日期:2019-11-08 00:00:00
abstract::Cryo-electron microscopy can determine the structure of biological matter in vitrified liquids. However, structure alone is insufficient to understand the function of native and engineered biomolecules. So far, their mechanical properties have mainly been probed at room temperature using tens of pico-newton forces wit...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-08531-4
更新日期:2019-02-08 00:00:00
abstract::Acenes are an important class of polycyclic aromatic hydrocarbons which have recently gained exceptional attention due to their potential as functional organic semiconductors. Fundamentally, they are important systems to study the convergence of physico-chemical properties of all-carbon sp2-frameworks in the one-dimen...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-08650-y
更新日期:2019-02-20 00:00:00
abstract::A challenge for neuroscience is to understand the conscious and unconscious processes underlying construction of willed actions. We investigated the neural substrate of human motor awareness during awake brain surgery. In a first experiment, awake patients performed a voluntary hand motor task and verbally monitored t...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-14517-4
更新日期:2020-02-04 00:00:00
abstract::Hundreds of clinical studies have demonstrated associations between the human microbiome and disease, yet fundamental questions remain on how we can generalize this knowledge. Results from individual studies can be inconsistent, and comparing published data is further complicated by a lack of standard processing and a...
journal_title:Nature communications
pub_type: 杂志文章,meta分析
doi:10.1038/s41467-017-01973-8
更新日期:2017-12-05 00:00:00
abstract::Liquids cooled towards the glass transition temperature transform into amorphous solids that have a wide range of applications. While the nature of this transformation is understood rigorously in the mean-field limit of infinite spatial dimensions, the problem remains wide open in physical dimensions. Nontrivial finit...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-09512-3
更新日期:2019-04-03 00:00:00
abstract::Turning graphene magnetic is a promising challenge to make it an active material for spintronics. Predictions state that graphene structures with specific shapes can spontaneously develop magnetism driven by Coulomb repulsion of π-electrons, but its experimental verification is demanding. Here, we report on the observ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-08060-6
更新日期:2019-01-14 00:00:00
abstract::Circadian clock mechanisms have been extensively investigated but the main rate-limiting step that determines circadian period remains unclear. Formation of a stable complex between clock proteins and CK1 is a conserved feature in eukaryotic circadian mechanisms. Here we show that the FRQ-CK1 interaction, but not FRQ ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-12239-w
更新日期:2019-09-25 00:00:00