Abstract：
:An immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) for ultrasensitive determination of Sudan I in food samples was reported. Gold-silver core-shell bimetallic nanorods (referred to as Au@Ag NRs) were synthesized, characterized and used as the substrate for preparation of the ICA. Polyclonal antibody against Sudan I was immobilized on the surface of the Au@Ag NRs carrying the Raman reporter 5,5'-dithiobis (2-nitrobenzoic acid). The Raman scattering intensity on the test line was used for quantitation of Sudan I. The assay was completed in 15 min. IC50 and limit of detection (LOD) were 30 pg mL-1 and 0.2 pg mL-1, respectively. There was no cross-reactivity (CR) of the assay with Sunset Yellow, Lemon Yellow and Brilliant blue FCF, but only 3.53%-9.74% CR with Sudan II, III and IV. The recoveries of Sudan I from spiked food samples were in the range of 88.9-107.6% with relative standard deviation of 3.7-8.7% (n = 3).
journal_name
Food Chemjournal_title
Food chemistryauthors
Deng D,Yang H,Liu C,Zhao K,Li J,Deng Adoi
10.1016/j.foodchem.2018.10.129subject
Has Abstractpub_date
2019-03-30 00:00:00pages
595-603eissn
0308-8146issn
1873-7072pii
S0308-8146(18)31914-9journal_volume
277pub_type
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